Review Articles
Vol. 13 No. sp5 (2026): Recent Advances in Agriculture
Pesticide residues in leafy vegetables: From detection to detoxification
Department of Agricultural Entomology, College of Agriculture, Vellayani, Thiruvananthapuram 695 522, Kerala, India
All India Network Project (AINP) on Pesticide Residues and Contaminants, Department of Agricultural Entomology, College of Agriculture, Vellayani, Thiruvananthapuram 695 522, Kerala, India
Abstract
Leafy vegetables constitute a vital component of human diets worldwide owing to their rich nutritional profile, including essential vitamins, minerals, dietary fibre and bioactive compounds. However, their cultivation often involves intensive pesticide application, which may leave harmful residues on edible tissues. The persistence of these residues poses significant public health concerns due to their potential for acute and chronic toxicity. This review compiles and synthesises current research findings on pesticide residues in major leafy vegetables, including lettuce, cabbage, spinach, amaranthus, curry leaf, celery, rocket and parsley. Special emphasis is placed on residue monitoring studies across different countries, on dissipation patterns under varying agroecological conditions and on household- or field-level decontamination strategies. This review highlights that multiple residues often exceed maximum residue limits (MRLs), raising risks for consumers. Dissipation studies show that half-lives vary widely depending on the chemical, crop and environmental conditions. At the same time, effective decontamination requires tailored interventions such as washing, cooking and using salt, vinegar or commercial washes. Overall, reducing pesticide residues in leafy vegetables requires integrated monitoring approaches, improved agricultural practices and scientifically validated decontamination methods to safeguard consumer health.
References
- 1. Alqahtani D, Al-Mutairi M, Alrashed N, Alajmi R, Alowaifeer A. Determination of pesticide residues in leafy vegetables and dietary risk assessment. J Toxicol Risk Assess. 2023;9:056. https://doi.org/10.23937/2572-4061.1510056
- 2. Deveci B, Golge O, Kabak B. Quantification of 363 pesticides in leafy vegetables (dill, rocket and parsley) in the Turkish market by using QuEChERS with LC-MS/MS and GC-MS/MS. Foods. 2023;12(5):1034. https://doi.org/10.3390/foods12051034
- 3. Atitsogbey P, Kyereh E, Ofori H, Johnson PNT, Steiner-Asiedu M. Heavy metal, microbial and pesticide residue contaminations are limiting the potential consumption of green leafy vegetables in Ghana: an overview. Heliyon. 2023;9(4):e15466. https://doi.org/10.1016/j.heliyon.2023.e15466
- 4. Elakkiya S, Suganthy M, Bhuvaneswari K, Rajasree V. Assessment of farmer's perception on pesticide usage pattern and knowledge on pest management in amaranthus in Coimbatore district. Madras Agric J. 2021;108(Special):1. https://doi.org/10.29321/MAJ.10.000537
- 5. ICMR-National Institute of Nutrition. Indian Council of Medical Research dietary guidelines for Indians [Internet]. 2024. https://www.nin.res.in/dietaryguidelines/pdfjs/locale/DGI07052024P.pdf
- 6. Losio MN, Pavoni E, Bilei S, Bertasi B, Bove D, Capuano F, et al. Microbiological survey of raw and ready-to-eat leafy green vegetables marketed in Italy. Int J Food Microbiol. 2015;210:88–91. https://doi.org/10.1016/j.ijfoodmicro.2015.05.026
- 7. Zhang Q, Ying Z, Tang T, Guo B, Gu S, Fu L, et al. Residual characteristics and potential integrated risk assessment of synthetic pyrethroids in leafy vegetables from Zhejiang in China-based on a 3-year investigation. Food Chem. 2021;365:130389. https://doi.org/10.1016/j.foodchem.2021.130389
- 8. Abah M, Olawale O, Okoli EC, Emmanuel OP, Bando DC, Shenia ZH. Determination of selected pesticide residues in leafy vegetables (Amaranthus spinosus) consumed in Donga, Taraba State. IJBBSS. 2021;6(2):9–16. https://doi.org/10.37745/ijbbbs.15
- 9. Sanborn M, Cole D, Kerr K, Vakil C, Sanin H, Bassil K. Systematic review of pesticide human health effects. Ontario College of Family Physicians; 2004.
- 10. Dalvie MA, London L. Risk assessment of pesticide residues in South African raw wheat. Crop Prot. 2009;28:864–69. https://doi.org/10.1016/j.cropro.2009.07.008
- 11. Alavanja MCR, Matthew KR, Matthew RB. Increased cancer burden among pesticide applicators and others due to pesticide exposure. CA Cancer J Clin. 2013;63:120–42. https://doi.org/10.3322/caac.21170
- 12. Anastassiades M, Lehotay SJ, Stajnbaher D, Schenck FJ. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and dispersive solid-phase extraction for the determination of pesticide residues in produce. J AOAC Int. 2003;86(2):412–31. https://doi.org/10.1093/jaoac/86.2.412
- 13. Satpathy G, Tyagi YK, Gupta RK. Removal of organophosphorus (OP) pesticide residues from vegetables using washing solutions and boiling. J Agric Sci. 2012;4(2):69–78. https://doi.org/10.5539/jas.v4n2p69
- 14. Kar A, Mandal K, Singh B. Decontamination of chlorantraniliprole residues on cabbage and cauliflower through household processing methods. Bull Environ Contam Toxicol. 2012;88(4):501–06. https://doi.org/10.1007/s00128-012-0534-x
- 15. Dogheim SM, Ashraf EM, Alla SG, Khorshid MA, Fahmy SM. Pesticides and heavy metals levels in Egyptian leafy vegetables and some aromatic medicinal plants. Food Addit Contam. 2004;21(4):323–30. https://doi.org/10.1080/02652030310001656361
- 16. Amoah P, Drechsel P, Abaidoo RC, Ntow WJ. Pesticide and pathogen contamination of vegetables in Ghana's urban markets. Arch Environ Contam Toxicol. 2006;50:1–6. https://doi.org/10.1007/s00244-004-0054-8
- 17. Selim MT, El-Saeid MH, Al-Dossari IM. Multi-residues analysis of pesticides using gas chromatography mass spectrometry: I-leafy vegetables. Res J Environ Sci. 2011;5(3):248–58. https://doi.org/10.3923/rjes.2011.248.258
- 18. Bempah CK, Buah-Kwofie A, Enimil E, Blewu B, Agyei-Martey G. Residues of organochlorine pesticides in vegetables marketed in Greater Accra region of Ghana. Food Control. 2012;25(2):537–42. https://doi.org/10.1016/j.foodcont.2011.11.035
- 19. Osei-Fosu P, Donkor AK, Nyarko S, Nazzah NK, Asante IK, Kingsford-Adabo R, et al. Monitoring of pesticide residues of five notable vegetables at Agbogbloshie market in Accra, Ghana. Environ Monit Assess. 2014;186:7157–63. https://doi.org/10.1007/s10661-014-3917-0
- 20. Ma C, Wei D, Liu P, Fan K, Nie L, Song Y, et al. Pesticide residues in commonly consumed vegetables in Henan Province of China in 2020. Front Public Health. 2022;10:901485. https://doi.org/10.3389/fpubh.2022.901485
- 21. Sapbamrer R, Hongsibsong S. Organophosphorus pesticide residues in vegetables from farms, markets and a supermarket around Kwan Phayao Lake of northern Thailand. Arch Environ Contam Toxicol. 2014;67:60–67. https://doi.org/10.1007/s00244-014-0014-x
- 22. Yu R, Liu Q, Liu J, Wang Q, Wang Y. Concentrations of organophosphorus pesticides in fresh vegetables and related human health risk assessment in Changchun, Northeast China. Food Control. 2016;60:353–60. https://doi.org/10.1016/j.foodcont.2015.08.013
- 23. Fosu PO, Donkor A, Ziwu C, Dubey B, Kingsford-Adaboh R, Asante I, et al. Surveillance of pesticide residues in fruits and vegetables from Accra Metropolis markets, Ghana, 2010–2012: a case study in Sub-Saharan Africa. Environ Sci Pollut Res Int. 2017;24(20):17187–205. https://doi.org/10.1007/s11356-017-9287-8
- 24. Micah MM, Peace Z, Usaku R, Onyebuchi PU. Assessment of heavy metals and pesticide residues in Abelmoschus esculentus (Okro), Amaranthus cruentus (Spinach), Hibiscus sabdariffa (Roselle) and cabbage vegetables from agricultural area of Boronji Jimeta, Adamawa State, Nigeria. J Appl Sci Environ Manag. 2024;28(7).
- 25. Charan PD, Ali SF, Kachhawa Y, Sharma KC. Monitoring of pesticide residues in farmgate vegetables of central Aravalli region of western India. Am Eurasian J Agric Environ Sci. 2010;7(3):255–8.
- 26. Kerala Department of Agriculture Development and Farmers Welfare. Government of Kerala [Internet]. 2013–2021.
- 27. Rahman M, Hoque MS, Bhowmik S, Ferdousi S, Kabiraz MP, van Brakel ML. Monitoring of pesticide residues from fish feed, fish and vegetables in Bangladesh by GC-MS using the QuEChERS method. Heliyon. 2021;7(3):e06390. https://doi.org/10.1016/j.heliyon.2021.e06390
- 28. Omeje KO, Ezema BO, Okonkwo F, Onyishi NC, Ozioko J, Rasaq WA, et al. Quantification of heavy metals and pesticide residues in widely consumed Nigerian food crops using atomic absorption spectroscopy (AAS) and gas chromatography (GC). Toxins. 2021;13(12):870. https://doi.org/10.3390/toxins13120870
- 29. Kelle HI, Ogoko EC, Abiola OO, Achem D, Udeozo IP. Monitoring and health risk assessment of organochlorine pesticide residue in some leafy and fruiting vegetables from Lagos State, Southwestern Nigeria. J Kenya Chem Soc. 2022;15(1):3–13.
- 30. Muralikrishna P. Management of pesticide residues in vegetable amaranth (Amaranthus tricolor L.) [MSc dissertation]. Kerala Agricultural University; 2015.
- 31. Suneth RF, Dadang D, Nurmansyah A. Residues of several active insecticide ingredients in spinach (Amaranthus tricolor L.) and kale (Ipomoea reptans (L.) Poir.) in Bogor, West Java. In: Bio Web of Conferences. 2024;127:04002.
- 32. Nair KP, Mathew TB, Beevi SN, George T, Rajith R. Monitoring and risk assessment of pesticide residues in agricultural/horticultural commodities. Entomon. 2013;38(3):119–30.
- 33. Rani S, Vemuri SS, Reddy VV. Determination of pesticide residues in curry leaf in local markets of Hyderabad, India. IJEAB. 2016;1(3):238543. https://doi.org/10.22161/ijeab/1.3.12
- 34. Kaithamalai B, Palanisamy K, Chellamuthu S, Pandi T, Samygounder I, Venkidusamy M. Dissipation kinetics and decontamination of chlorantraniliprole and thiamethoxam residues in curry leaves. Int J Environ Anal Chem. 2024:1–18. https://doi.org/10.1080/03067319.2024.2386346
- 35. Siddiqui A, Zanki AASA, Al Muharrami AAH, Jagadeesan P. Is the culinary effect of curry leaves hampered by pesticide residues? A case study. ChemRxiv [Preprint]. 2025. https://doi.org/10.26434/chemrxiv-2025-l6z5n
- 36. Fang L, Zhang S, Chen Z, Du H, Zhu Q, Dong Z, et al. Risk assessment of pesticide residues in dietary intake of celery in China. Regul Toxicol Pharmacol. 2015;73(2):578–86. https://doi.org/10.1016/j.yrtph.2015.08.009
- 37. Tang H, Wu Y, Cheng Y, Zhao M, Tang T. Pesticide residue levels and cumulative acute dietary intake risk assessment of celery in Zhejiang Province. Chin J Pestic Sci. 2021;23(5):947–55. https://doi.org/10.16801/j.issn.1008-7303.2021.0092
- 38. Fan S, Zhang F, Deng K, Yu C, Liu S, Zhao P, et al. Spinach or amaranth contains highest residue of metalaxyl, fluazifop-P-butyl, chlorpyrifos and lambda-cyhalothrin on six leaf vegetables upon open field application. J Agric Food Chem. 2013;61(9):2039–44. https://doi.org/10.1021/jf304710u
- 39. Van Le T, Nguyen HT. Dissipation dynamics and half-lives of cypermethrin, emamectin benzoate and indoxacarb insecticides in different parts of amaranth (Amaranthus tricolor L.) and mustard greens (Brassica juncea). Trop Agric. 2021;98(1).
- 40. Guan Y, Huang F, Ma C, Fan J, Hao G. Dissipation and residues of imidacloprid in amaranth under greenhouse and open field cultivations. J Environ Sci Health B. 2024;59(7):390–8. https://doi.org/10.1080/03601234.2024.2356991
- 41. Aung O, Soe OO, Sein NN, Nyaing K, Myint A. Degradation of dimethoate and diazinon pesticide residues in Amaranthus tricolor L. (spinach) and soil under pesticide-treated plants. In: 2nd Myanmar Korea Conference Research Journal; 2019. p. 150–6.
- 42. Shalikram MSS. Studies on bioefficacy and residues of insecticides in major leafy vegetables [PhD dissertation]. Mahatma Phule Krishi Vidyapeeth, Maharashtra; 2023.
- 43. Gopalakrishnan R, Bhuvaneswari K, Kousika J, Manivannan A, Suganthi A. Persistence and dissipation pattern of dimethoate 30 EC in/on foxtail amaranthus and spinach. Madras Agric J. 2018;105(1–3):78–83.
- 44. Gajbhiye VT, Gupta S, Gupta RK. Persistence of imidacloprid in/on cabbage and cauliflower. Bull Environ Contam Toxicol. 2004;72:283–8. https://doi.org/10.1007/s00128-003-9103-7
- 45. Sharma A, Srivastava A, Ram B, Srivastava PC. Dissipation behaviour of spinosad insecticide in soil, cabbage and cauliflower under subtropical conditions. Pest Manag Sci. 2007;63:1141–5. https://doi.org/10.1002/ps.1437
- 46. Zhang ZY, Liu XJ, Hong XY. Effects of home preparation on pesticide residues in cabbage. Food Control. 2007;18:1484–7. https://doi.org/10.1016/j.foodcont.2006.11.002
- 47. Ditya P, Sarkar SP. Degradation dynamics of chlorfenapyr residue in chilli, cabbage and soil. Bull Environ Contam Toxicol. 2010;84:602–6. https://doi.org/10.1007/s00128-010-9994-z
- 48. Mohapatra S, Ahuja AK, Deepa M, Sharma D, Jagadish GK, Rashmi N. Persistence and dissipation of flubendiamide and des-iodoflubendiamide in cabbage (Brassica oleracea L.) and soil. Bull Environ Contam Toxicol. 2010;85(3):352–6. https://doi.org/10.1007/s00128-010-0063-4
- 49. Chahil GS, Singh G, Bhardwaj U, Takkar R, Ramindeijit S. Degradation dynamics of quinalphos on cabbage under subtropical conditions of Ludhiana, Punjab. Orbital Electron J Chem. 2011;3(2):35–9. https://doi.org/10.17807/orbital.v3i2.215
- 50. Padmanabhan A, Paul A. Persistence and risk assessment of quinalphos 25% EC in cabbage under plains and hills. In: Abstracts, International Conference on Advances in Agriculture and Allied Science Technologies for Sustainable Development; Hyderabad. Genesis Urban and Rural Development Society; 2018. p. 421. Abstract No. PP-SPHM-13.
- 51. Beevi SN, Paul A, George T, Pratheeshkumar N, Xavier G, Raj VS. Dissipation of chlorpyriphos and profenophos in cabbage (Brassica oleracea var. capitata L.). Pestic Res J. 2018;30(1):121–5. https://doi.org/10.5958/2249-524X.2018.00021.3
- 52. Padmanabhan A. Dissipation and risk assessment of select insecticides used for pest management in cabbage and cauliflower [PhD dissertation]. Kerala Agricultural University; 2018.
- 53. Sharma KK, Tripathy V, Mohapatra S, Matadha NY, Pathan ARK, Sharma BN, et al. Dissipation kinetics and consumer risk assessment of novaluron + lambda-cyhalothrin co-formulation in cabbage. Ecotoxicol Environ Saf. 2021;208:111494. https://doi.org/10.1016/j.ecoenv.2020.111494
- 54. Gao Q, Hu J, Li Y, Liang Y, Zhang Z. Dissipation and residue distribution of imidacloprid, difenoconazole and chlorothalonil in cabbage. Chin J Pestic Sci. 2021;23(6):1213–8.
- 55. Sawant CG, Guru PN, Mundhe SG, Patil CS, Sadhukhan R. Method validation and dissipation behaviour of ethion 50 EC insecticide in winter cabbage. Pharma Innov J. 2022;11(12):5825–9.
- 56. Akoijam R, Ningombam A, Sonia C, Devi CP, Singh TR, Singh IM. Dissipation of insecticidal residues having different modes of action in cabbage. Research Square [Preprint]. 2023. https://doi.org/10.21203/rs.3.rs-3294472/v1
- 57. Yu J, Hou J, Xu Z, Yu R, Zhang C, Chen L, et al. Dissipation behaviour and dietary risk assessment of cyclaniliprole and its metabolite in cabbage under field conditions. Environ Sci Pollut Res. 2023;30(60):125907–14. https://doi.org/10.1007/s11356-023-31146-8
- 58. Liu X, Ban N, Fu Z, Gao X, Liu TX, Liang P. Persistent toxicity and dissipation dynamics of afidopyropen against the green peach aphid Myzus persicae (Sulzer) in cabbage and chilli. Ecotoxicol Environ Saf. 2023;252:114584.
- 59. Bhartiya S, Katna S, Dubey JK, Sharma A, Sharma S. Residue dynamics of some insecticides in cabbage and soil. Pestic Res J. 2023;35(1):80–5.
- 60. Cao J, Lv Y, Qi Y, Qin S, Wang X, Li J. Dissipation, terminal residue and dietary risk assessment of flonicamid in cabbage. Int J Environ Anal Chem. 2024;104(18):6886–97. https://doi.org/10.1080/03067319.2022.2155050
- 61. Sun C, Chen L, Liu Y, Zheng W, Hua Y, Zhang Q. Dissipation behaviour and risk assessment of three pesticide residues under combined application in greenhouse-grown cabbage. Foods. 2025;14(17):3006.
- 62. Majumder S, Pandey J, Kumar A, Maurya S, Srivastava K, Nath Singh A. Residue dissipation kinetics, risk assessment and decontamination of spiromesifen in tomato fruits and cabbage heads. J Agric Sci Technol. 2025;27(5):1185–98.
- 63. Anuradha P, Bhuvaneswari K. Dissipation and persistence of chlorpyriphos 20 EC in/on curry leaf. Madras Agric J. 2016;103. https://doi.org/10.29321/MAJ.10.001009
- 64. Priyadarshini G, Vemuri S, Reddy N. Dissipation pattern of carbendazim and cypermethrin on curry leaf. Int J Environ Agric Res. 2017;3(1).
- 65. Ratnamma R, Naik MSH, Pallavi M, Bheemanna. Dissipation and decontamination of dimethoate in curry leaf using LC-MS/MS (ESI) technique. Pestic Res J. 2021;33(1). https://doi.org/10.5958/2249-524X.2021.00016.9
- 66. Pasar R, Pallavi MS, Harischandra NR, Devaraj M, Nandini P, Bheemanna M, et al. Simultaneous determination of dimethoate and its metabolite omethoate in curry leaf using LC-MS/MS and risk assessment. J Sep Sci. 2022;45(11):1831–8. https://doi.org/10.1002/jssc.202100696
- 67. Pan L, Feng X, Zhang H. Dissipation and residues of pyrethrins in leaf lettuce under greenhouse and open field conditions. Int J Environ Res Public Health. 2017;14(7):822. https://doi.org/10.3390/ijerph14070822
- 68. Lu MX, Jiang WW, Wang JL, Jian Q, Shen Y, Liu XJ, et al. Persistence and dissipation of chlorpyrifos in Brassica chinensis, lettuce, celery, asparagus lettuce, eggplant and pepper in a greenhouse. PLoS One. 2014;9(6):e100556. https://doi.org/10.1371/journal.pone.0100556
- 69. Yang SJ, Mun S, Kim HJ, Han SJ, Kim DW, Cho BS, et al. Effectiveness of different washing strategies on pesticide residue removal: the first comparative study on leafy vegetables. Foods. 2022;11(18):2916. https://doi.org/10.3390/foods11182916
- 70. Kang SM, Lee MG. Fate of some pesticides during brining and cooking of Chinese cabbage and spinach. Food Sci Biotechnol. 2005;14(1):77–81.
- 71. Aktar MW, Sengupta D, Purkait S, Chowdhury A. Risk assessment and decontamination of quinalphos under different culinary processes in/on cabbage. Environ Monit Assess. 2010;163(1–4):369–77. https://doi.org/10.1007/s10661-009-0841-9
- 72. Ling Y, Wang H, Yong W, Zhang P, Sim L, Yang ML, et al. The effects of washing and cooking on chlorpyrifos and its toxic metabolites in vegetables. Food Control. 2011;22:54–8. https://doi.org/10.1016/j.foodcont.2010.06.009
- 73. Khagi S. Assessing washing methods for reduction of pesticide residues in green leafy vegetables. KJour. 2023;5(2):91–102. https://doi.org/10.3126/kjour.v5i2.60444
- 74. Nair KP, Mathew TB, Beevi SN, George T. Monitoring and decontamination of pesticide residues in okra (Abelmoschus esculentus Moench). Int J Interdiscip Multidiscip Stud. 2014;1(5):242–8.
- 75. Swarupa S, Vemuri S, Reddy VV, Kavitha K. Risk mitigation for removal of pesticide residues in curry leaf for food safety. IJEAB. 2016;1(4):238596.
- 76. Paramasivam M. Determination of synthetic pyrethroids and hexaconazole residues in curry leaves and decontamination through household techniques. J Food Sci Technol. 2022;59(4):1549–57. https://doi.org/10.1007/s13197-021-05165-7
- 77. Randhawa MA, Anjum FM, Asi MR, Butt MS, Ahmed A, Randhawa MS. Removal of endosulfan residues from vegetables by household processing. J Sci Ind Res. 2007;66:849–52.
- 78. Amir RM, Randhawa MA, Nadeem M, Ahmed A, Ahmad A, Khan MR, et al. Assessing and reporting household chemicals as a novel tool to mitigate pesticide residues in spinach (Spinacia oleracea). Sci Rep. 2019;9(1):1125. https://doi.org/10.1038/s41598-018-37936-2
- 79. Aaruni PS. Management of pesticide residues in select spices [MSc dissertation]. Kerala Agricultural University; 2016.
- 80. Muralikrishna P, Mathew TB, Sreelakshmi P, Koshy BA, Paul A, Rajith R. Evaluation of different household practices to decontaminate organophosphate insecticide residues from Amaranthus tricolor L. Entomon. 2016;41(3):195–202. https://doi.org/10.33307/entomon.v41i3.181
- 81. Muralikrishna P, Mathew TB, Nithya PR, Paul A. Evaluation of different household practice to decontaminate synthetic pyrethroid insecticide residues from Amaranthus tricolor L. IJCS. 2019;7(5):2049–52.
- 82. Balkan T, Yılmaz O. Efficacy of some washing solutions for removal of pesticide residues in lettuce. Beni-Suef Univ J Basic Appl Sci. 2022;11(1):143. https://doi.org/10.1186/s43088-022-00324-x
- 83. Chen Q, Wang Y, Chen F, Zhang Y, Liao X. Chlorine dioxide treatment for the removal of pesticide residues on fresh lettuce and in aqueous solution. Food Control. 2014;40:106–12. https://doi.org/10.1016/j.foodcont.2013.11.035
- 84. Park DW, Yang YS, Lee YU, Han SJ, Kim HJ, Kim SH, et al. Pesticide residues and risk assessment from monitoring programmes in the largest production area of leafy vegetables in South Korea: a 15-year study. Foods. 2021;10(2):425. https://doi.org/10.3390/foods10020425
Downloads
Download data is not yet available.