Skip to main navigation menu Skip to main content Skip to site footer

Review Articles

Vol. 13 No. sp5 (2026): Recent Advances in Agriculture

Pesticide residues in leafy vegetables: From detection to detoxification

DOI
https://doi.org/10.14719/pst.13940
Submitted
1 February 2026
Published
15-09-2026

Abstract

Leafy vegetables constitute a vital component of human diets worldwide owing to their rich nutritional profile, including essential vitamins, minerals, dietary fibre and bioactive compounds. However, their cultivation often involves intensive pesticide application, which may leave harmful residues on edible tissues. The persistence of these residues poses significant public health concerns due to their potential for acute and chronic toxicity. This review compiles and synthesises current research findings on pesticide residues in major leafy vegetables, including lettuce, cabbage, spinach, amaranthus, curry leaf, celery, rocket and parsley. Special emphasis is placed on residue monitoring studies across different countries, on dissipation patterns under varying agroecological conditions and on household- or field-level decontamination strategies. This review highlights that multiple residues often exceed maximum residue limits (MRLs), raising risks for consumers. Dissipation studies show that half-lives vary widely depending on the chemical, crop and environmental conditions. At the same time, effective decontamination requires tailored interventions such as washing, cooking and using salt, vinegar or commercial washes. Overall, reducing pesticide residues in leafy vegetables requires integrated monitoring approaches, improved agricultural practices and scientifically validated decontamination methods to safeguard consumer health.

References

  1. 1. Alqahtani D, Al-Mutairi M, Alrashed N, Alajmi R, Alowaifeer A. Determination of pesticide residues in leafy vegetables and dietary risk assessment. J Toxicol Risk Assess. 2023;9:056. https://doi.org/10.23937/2572-4061.1510056
  2. 2. Deveci B, Golge O, Kabak B. Quantification of 363 pesticides in leafy vegetables (dill, rocket and parsley) in the Turkish market by using QuEChERS with LC-MS/MS and GC-MS/MS. Foods. 2023;12(5):1034. https://doi.org/10.3390/foods12051034
  3. 3. Atitsogbey P, Kyereh E, Ofori H, Johnson PNT, Steiner-Asiedu M. Heavy metal, microbial and pesticide residue contaminations are limiting the potential consumption of green leafy vegetables in Ghana: an overview. Heliyon. 2023;9(4):e15466. https://doi.org/10.1016/j.heliyon.2023.e15466
  4. 4. Elakkiya S, Suganthy M, Bhuvaneswari K, Rajasree V. Assessment of farmer's perception on pesticide usage pattern and knowledge on pest management in amaranthus in Coimbatore district. Madras Agric J. 2021;108(Special):1. https://doi.org/10.29321/MAJ.10.000537
  5. 5. ICMR-National Institute of Nutrition. Indian Council of Medical Research dietary guidelines for Indians [Internet]. 2024. https://www.nin.res.in/dietaryguidelines/pdfjs/locale/DGI07052024P.pdf
  6. 6. Losio MN, Pavoni E, Bilei S, Bertasi B, Bove D, Capuano F, et al. Microbiological survey of raw and ready-to-eat leafy green vegetables marketed in Italy. Int J Food Microbiol. 2015;210:88–91. https://doi.org/10.1016/j.ijfoodmicro.2015.05.026
  7. 7. Zhang Q, Ying Z, Tang T, Guo B, Gu S, Fu L, et al. Residual characteristics and potential integrated risk assessment of synthetic pyrethroids in leafy vegetables from Zhejiang in China-based on a 3-year investigation. Food Chem. 2021;365:130389. https://doi.org/10.1016/j.foodchem.2021.130389
  8. 8. Abah M, Olawale O, Okoli EC, Emmanuel OP, Bando DC, Shenia ZH. Determination of selected pesticide residues in leafy vegetables (Amaranthus spinosus) consumed in Donga, Taraba State. IJBBSS. 2021;6(2):9–16. https://doi.org/10.37745/ijbbbs.15
  9. 9. Sanborn M, Cole D, Kerr K, Vakil C, Sanin H, Bassil K. Systematic review of pesticide human health effects. Ontario College of Family Physicians; 2004.
  10. 10. Dalvie MA, London L. Risk assessment of pesticide residues in South African raw wheat. Crop Prot. 2009;28:864–69. https://doi.org/10.1016/j.cropro.2009.07.008
  11. 11. Alavanja MCR, Matthew KR, Matthew RB. Increased cancer burden among pesticide applicators and others due to pesticide exposure. CA Cancer J Clin. 2013;63:120–42. https://doi.org/10.3322/caac.21170
  12. 12. Anastassiades M, Lehotay SJ, Stajnbaher D, Schenck FJ. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and dispersive solid-phase extraction for the determination of pesticide residues in produce. J AOAC Int. 2003;86(2):412–31. https://doi.org/10.1093/jaoac/86.2.412
  13. 13. Satpathy G, Tyagi YK, Gupta RK. Removal of organophosphorus (OP) pesticide residues from vegetables using washing solutions and boiling. J Agric Sci. 2012;4(2):69–78. https://doi.org/10.5539/jas.v4n2p69
  14. 14. Kar A, Mandal K, Singh B. Decontamination of chlorantraniliprole residues on cabbage and cauliflower through household processing methods. Bull Environ Contam Toxicol. 2012;88(4):501–06. https://doi.org/10.1007/s00128-012-0534-x
  15. 15. Dogheim SM, Ashraf EM, Alla SG, Khorshid MA, Fahmy SM. Pesticides and heavy metals levels in Egyptian leafy vegetables and some aromatic medicinal plants. Food Addit Contam. 2004;21(4):323–30. https://doi.org/10.1080/02652030310001656361
  16. 16. Amoah P, Drechsel P, Abaidoo RC, Ntow WJ. Pesticide and pathogen contamination of vegetables in Ghana's urban markets. Arch Environ Contam Toxicol. 2006;50:1–6. https://doi.org/10.1007/s00244-004-0054-8
  17. 17. Selim MT, El-Saeid MH, Al-Dossari IM. Multi-residues analysis of pesticides using gas chromatography mass spectrometry: I-leafy vegetables. Res J Environ Sci. 2011;5(3):248–58. https://doi.org/10.3923/rjes.2011.248.258
  18. 18. Bempah CK, Buah-Kwofie A, Enimil E, Blewu B, Agyei-Martey G. Residues of organochlorine pesticides in vegetables marketed in Greater Accra region of Ghana. Food Control. 2012;25(2):537–42. https://doi.org/10.1016/j.foodcont.2011.11.035
  19. 19. Osei-Fosu P, Donkor AK, Nyarko S, Nazzah NK, Asante IK, Kingsford-Adabo R, et al. Monitoring of pesticide residues of five notable vegetables at Agbogbloshie market in Accra, Ghana. Environ Monit Assess. 2014;186:7157–63. https://doi.org/10.1007/s10661-014-3917-0
  20. 20. Ma C, Wei D, Liu P, Fan K, Nie L, Song Y, et al. Pesticide residues in commonly consumed vegetables in Henan Province of China in 2020. Front Public Health. 2022;10:901485. https://doi.org/10.3389/fpubh.2022.901485
  21. 21. Sapbamrer R, Hongsibsong S. Organophosphorus pesticide residues in vegetables from farms, markets and a supermarket around Kwan Phayao Lake of northern Thailand. Arch Environ Contam Toxicol. 2014;67:60–67. https://doi.org/10.1007/s00244-014-0014-x
  22. 22. Yu R, Liu Q, Liu J, Wang Q, Wang Y. Concentrations of organophosphorus pesticides in fresh vegetables and related human health risk assessment in Changchun, Northeast China. Food Control. 2016;60:353–60. https://doi.org/10.1016/j.foodcont.2015.08.013
  23. 23. Fosu PO, Donkor A, Ziwu C, Dubey B, Kingsford-Adaboh R, Asante I, et al. Surveillance of pesticide residues in fruits and vegetables from Accra Metropolis markets, Ghana, 2010–2012: a case study in Sub-Saharan Africa. Environ Sci Pollut Res Int. 2017;24(20):17187–205. https://doi.org/10.1007/s11356-017-9287-8
  24. 24. Micah MM, Peace Z, Usaku R, Onyebuchi PU. Assessment of heavy metals and pesticide residues in Abelmoschus esculentus (Okro), Amaranthus cruentus (Spinach), Hibiscus sabdariffa (Roselle) and cabbage vegetables from agricultural area of Boronji Jimeta, Adamawa State, Nigeria. J Appl Sci Environ Manag. 2024;28(7).
  25. 25. Charan PD, Ali SF, Kachhawa Y, Sharma KC. Monitoring of pesticide residues in farmgate vegetables of central Aravalli region of western India. Am Eurasian J Agric Environ Sci. 2010;7(3):255–8.
  26. 26. Kerala Department of Agriculture Development and Farmers Welfare. Government of Kerala [Internet]. 2013–2021.
  27. 27. Rahman M, Hoque MS, Bhowmik S, Ferdousi S, Kabiraz MP, van Brakel ML. Monitoring of pesticide residues from fish feed, fish and vegetables in Bangladesh by GC-MS using the QuEChERS method. Heliyon. 2021;7(3):e06390. https://doi.org/10.1016/j.heliyon.2021.e06390
  28. 28. Omeje KO, Ezema BO, Okonkwo F, Onyishi NC, Ozioko J, Rasaq WA, et al. Quantification of heavy metals and pesticide residues in widely consumed Nigerian food crops using atomic absorption spectroscopy (AAS) and gas chromatography (GC). Toxins. 2021;13(12):870. https://doi.org/10.3390/toxins13120870
  29. 29. Kelle HI, Ogoko EC, Abiola OO, Achem D, Udeozo IP. Monitoring and health risk assessment of organochlorine pesticide residue in some leafy and fruiting vegetables from Lagos State, Southwestern Nigeria. J Kenya Chem Soc. 2022;15(1):3–13.
  30. 30. Muralikrishna P. Management of pesticide residues in vegetable amaranth (Amaranthus tricolor L.) [MSc dissertation]. Kerala Agricultural University; 2015.
  31. 31. Suneth RF, Dadang D, Nurmansyah A. Residues of several active insecticide ingredients in spinach (Amaranthus tricolor L.) and kale (Ipomoea reptans (L.) Poir.) in Bogor, West Java. In: Bio Web of Conferences. 2024;127:04002.
  32. 32. Nair KP, Mathew TB, Beevi SN, George T, Rajith R. Monitoring and risk assessment of pesticide residues in agricultural/horticultural commodities. Entomon. 2013;38(3):119–30.
  33. 33. Rani S, Vemuri SS, Reddy VV. Determination of pesticide residues in curry leaf in local markets of Hyderabad, India. IJEAB. 2016;1(3):238543. https://doi.org/10.22161/ijeab/1.3.12
  34. 34. Kaithamalai B, Palanisamy K, Chellamuthu S, Pandi T, Samygounder I, Venkidusamy M. Dissipation kinetics and decontamination of chlorantraniliprole and thiamethoxam residues in curry leaves. Int J Environ Anal Chem. 2024:1–18. https://doi.org/10.1080/03067319.2024.2386346
  35. 35. Siddiqui A, Zanki AASA, Al Muharrami AAH, Jagadeesan P. Is the culinary effect of curry leaves hampered by pesticide residues? A case study. ChemRxiv [Preprint]. 2025. https://doi.org/10.26434/chemrxiv-2025-l6z5n
  36. 36. Fang L, Zhang S, Chen Z, Du H, Zhu Q, Dong Z, et al. Risk assessment of pesticide residues in dietary intake of celery in China. Regul Toxicol Pharmacol. 2015;73(2):578–86. https://doi.org/10.1016/j.yrtph.2015.08.009
  37. 37. Tang H, Wu Y, Cheng Y, Zhao M, Tang T. Pesticide residue levels and cumulative acute dietary intake risk assessment of celery in Zhejiang Province. Chin J Pestic Sci. 2021;23(5):947–55. https://doi.org/10.16801/j.issn.1008-7303.2021.0092
  38. 38. Fan S, Zhang F, Deng K, Yu C, Liu S, Zhao P, et al. Spinach or amaranth contains highest residue of metalaxyl, fluazifop-P-butyl, chlorpyrifos and lambda-cyhalothrin on six leaf vegetables upon open field application. J Agric Food Chem. 2013;61(9):2039–44. https://doi.org/10.1021/jf304710u
  39. 39. Van Le T, Nguyen HT. Dissipation dynamics and half-lives of cypermethrin, emamectin benzoate and indoxacarb insecticides in different parts of amaranth (Amaranthus tricolor L.) and mustard greens (Brassica juncea). Trop Agric. 2021;98(1).
  40. 40. Guan Y, Huang F, Ma C, Fan J, Hao G. Dissipation and residues of imidacloprid in amaranth under greenhouse and open field cultivations. J Environ Sci Health B. 2024;59(7):390–8. https://doi.org/10.1080/03601234.2024.2356991
  41. 41. Aung O, Soe OO, Sein NN, Nyaing K, Myint A. Degradation of dimethoate and diazinon pesticide residues in Amaranthus tricolor L. (spinach) and soil under pesticide-treated plants. In: 2nd Myanmar Korea Conference Research Journal; 2019. p. 150–6.
  42. 42. Shalikram MSS. Studies on bioefficacy and residues of insecticides in major leafy vegetables [PhD dissertation]. Mahatma Phule Krishi Vidyapeeth, Maharashtra; 2023.
  43. 43. Gopalakrishnan R, Bhuvaneswari K, Kousika J, Manivannan A, Suganthi A. Persistence and dissipation pattern of dimethoate 30 EC in/on foxtail amaranthus and spinach. Madras Agric J. 2018;105(1–3):78–83.
  44. 44. Gajbhiye VT, Gupta S, Gupta RK. Persistence of imidacloprid in/on cabbage and cauliflower. Bull Environ Contam Toxicol. 2004;72:283–8. https://doi.org/10.1007/s00128-003-9103-7
  45. 45. Sharma A, Srivastava A, Ram B, Srivastava PC. Dissipation behaviour of spinosad insecticide in soil, cabbage and cauliflower under subtropical conditions. Pest Manag Sci. 2007;63:1141–5. https://doi.org/10.1002/ps.1437
  46. 46. Zhang ZY, Liu XJ, Hong XY. Effects of home preparation on pesticide residues in cabbage. Food Control. 2007;18:1484–7. https://doi.org/10.1016/j.foodcont.2006.11.002
  47. 47. Ditya P, Sarkar SP. Degradation dynamics of chlorfenapyr residue in chilli, cabbage and soil. Bull Environ Contam Toxicol. 2010;84:602–6. https://doi.org/10.1007/s00128-010-9994-z
  48. 48. Mohapatra S, Ahuja AK, Deepa M, Sharma D, Jagadish GK, Rashmi N. Persistence and dissipation of flubendiamide and des-iodoflubendiamide in cabbage (Brassica oleracea L.) and soil. Bull Environ Contam Toxicol. 2010;85(3):352–6. https://doi.org/10.1007/s00128-010-0063-4
  49. 49. Chahil GS, Singh G, Bhardwaj U, Takkar R, Ramindeijit S. Degradation dynamics of quinalphos on cabbage under subtropical conditions of Ludhiana, Punjab. Orbital Electron J Chem. 2011;3(2):35–9. https://doi.org/10.17807/orbital.v3i2.215
  50. 50. Padmanabhan A, Paul A. Persistence and risk assessment of quinalphos 25% EC in cabbage under plains and hills. In: Abstracts, International Conference on Advances in Agriculture and Allied Science Technologies for Sustainable Development; Hyderabad. Genesis Urban and Rural Development Society; 2018. p. 421. Abstract No. PP-SPHM-13.
  51. 51. Beevi SN, Paul A, George T, Pratheeshkumar N, Xavier G, Raj VS. Dissipation of chlorpyriphos and profenophos in cabbage (Brassica oleracea var. capitata L.). Pestic Res J. 2018;30(1):121–5. https://doi.org/10.5958/2249-524X.2018.00021.3
  52. 52. Padmanabhan A. Dissipation and risk assessment of select insecticides used for pest management in cabbage and cauliflower [PhD dissertation]. Kerala Agricultural University; 2018.
  53. 53. Sharma KK, Tripathy V, Mohapatra S, Matadha NY, Pathan ARK, Sharma BN, et al. Dissipation kinetics and consumer risk assessment of novaluron + lambda-cyhalothrin co-formulation in cabbage. Ecotoxicol Environ Saf. 2021;208:111494. https://doi.org/10.1016/j.ecoenv.2020.111494
  54. 54. Gao Q, Hu J, Li Y, Liang Y, Zhang Z. Dissipation and residue distribution of imidacloprid, difenoconazole and chlorothalonil in cabbage. Chin J Pestic Sci. 2021;23(6):1213–8.
  55. 55. Sawant CG, Guru PN, Mundhe SG, Patil CS, Sadhukhan R. Method validation and dissipation behaviour of ethion 50 EC insecticide in winter cabbage. Pharma Innov J. 2022;11(12):5825–9.
  56. 56. Akoijam R, Ningombam A, Sonia C, Devi CP, Singh TR, Singh IM. Dissipation of insecticidal residues having different modes of action in cabbage. Research Square [Preprint]. 2023. https://doi.org/10.21203/rs.3.rs-3294472/v1
  57. 57. Yu J, Hou J, Xu Z, Yu R, Zhang C, Chen L, et al. Dissipation behaviour and dietary risk assessment of cyclaniliprole and its metabolite in cabbage under field conditions. Environ Sci Pollut Res. 2023;30(60):125907–14. https://doi.org/10.1007/s11356-023-31146-8
  58. 58. Liu X, Ban N, Fu Z, Gao X, Liu TX, Liang P. Persistent toxicity and dissipation dynamics of afidopyropen against the green peach aphid Myzus persicae (Sulzer) in cabbage and chilli. Ecotoxicol Environ Saf. 2023;252:114584.
  59. 59. Bhartiya S, Katna S, Dubey JK, Sharma A, Sharma S. Residue dynamics of some insecticides in cabbage and soil. Pestic Res J. 2023;35(1):80–5.
  60. 60. Cao J, Lv Y, Qi Y, Qin S, Wang X, Li J. Dissipation, terminal residue and dietary risk assessment of flonicamid in cabbage. Int J Environ Anal Chem. 2024;104(18):6886–97. https://doi.org/10.1080/03067319.2022.2155050
  61. 61. Sun C, Chen L, Liu Y, Zheng W, Hua Y, Zhang Q. Dissipation behaviour and risk assessment of three pesticide residues under combined application in greenhouse-grown cabbage. Foods. 2025;14(17):3006.
  62. 62. Majumder S, Pandey J, Kumar A, Maurya S, Srivastava K, Nath Singh A. Residue dissipation kinetics, risk assessment and decontamination of spiromesifen in tomato fruits and cabbage heads. J Agric Sci Technol. 2025;27(5):1185–98.
  63. 63. Anuradha P, Bhuvaneswari K. Dissipation and persistence of chlorpyriphos 20 EC in/on curry leaf. Madras Agric J. 2016;103. https://doi.org/10.29321/MAJ.10.001009
  64. 64. Priyadarshini G, Vemuri S, Reddy N. Dissipation pattern of carbendazim and cypermethrin on curry leaf. Int J Environ Agric Res. 2017;3(1).
  65. 65. Ratnamma R, Naik MSH, Pallavi M, Bheemanna. Dissipation and decontamination of dimethoate in curry leaf using LC-MS/MS (ESI) technique. Pestic Res J. 2021;33(1). https://doi.org/10.5958/2249-524X.2021.00016.9
  66. 66. Pasar R, Pallavi MS, Harischandra NR, Devaraj M, Nandini P, Bheemanna M, et al. Simultaneous determination of dimethoate and its metabolite omethoate in curry leaf using LC-MS/MS and risk assessment. J Sep Sci. 2022;45(11):1831–8. https://doi.org/10.1002/jssc.202100696
  67. 67. Pan L, Feng X, Zhang H. Dissipation and residues of pyrethrins in leaf lettuce under greenhouse and open field conditions. Int J Environ Res Public Health. 2017;14(7):822. https://doi.org/10.3390/ijerph14070822
  68. 68. Lu MX, Jiang WW, Wang JL, Jian Q, Shen Y, Liu XJ, et al. Persistence and dissipation of chlorpyrifos in Brassica chinensis, lettuce, celery, asparagus lettuce, eggplant and pepper in a greenhouse. PLoS One. 2014;9(6):e100556. https://doi.org/10.1371/journal.pone.0100556
  69. 69. Yang SJ, Mun S, Kim HJ, Han SJ, Kim DW, Cho BS, et al. Effectiveness of different washing strategies on pesticide residue removal: the first comparative study on leafy vegetables. Foods. 2022;11(18):2916. https://doi.org/10.3390/foods11182916
  70. 70. Kang SM, Lee MG. Fate of some pesticides during brining and cooking of Chinese cabbage and spinach. Food Sci Biotechnol. 2005;14(1):77–81.
  71. 71. Aktar MW, Sengupta D, Purkait S, Chowdhury A. Risk assessment and decontamination of quinalphos under different culinary processes in/on cabbage. Environ Monit Assess. 2010;163(1–4):369–77. https://doi.org/10.1007/s10661-009-0841-9
  72. 72. Ling Y, Wang H, Yong W, Zhang P, Sim L, Yang ML, et al. The effects of washing and cooking on chlorpyrifos and its toxic metabolites in vegetables. Food Control. 2011;22:54–8. https://doi.org/10.1016/j.foodcont.2010.06.009
  73. 73. Khagi S. Assessing washing methods for reduction of pesticide residues in green leafy vegetables. KJour. 2023;5(2):91–102. https://doi.org/10.3126/kjour.v5i2.60444
  74. 74. Nair KP, Mathew TB, Beevi SN, George T. Monitoring and decontamination of pesticide residues in okra (Abelmoschus esculentus Moench). Int J Interdiscip Multidiscip Stud. 2014;1(5):242–8.
  75. 75. Swarupa S, Vemuri S, Reddy VV, Kavitha K. Risk mitigation for removal of pesticide residues in curry leaf for food safety. IJEAB. 2016;1(4):238596.
  76. 76. Paramasivam M. Determination of synthetic pyrethroids and hexaconazole residues in curry leaves and decontamination through household techniques. J Food Sci Technol. 2022;59(4):1549–57. https://doi.org/10.1007/s13197-021-05165-7
  77. 77. Randhawa MA, Anjum FM, Asi MR, Butt MS, Ahmed A, Randhawa MS. Removal of endosulfan residues from vegetables by household processing. J Sci Ind Res. 2007;66:849–52.
  78. 78. Amir RM, Randhawa MA, Nadeem M, Ahmed A, Ahmad A, Khan MR, et al. Assessing and reporting household chemicals as a novel tool to mitigate pesticide residues in spinach (Spinacia oleracea). Sci Rep. 2019;9(1):1125. https://doi.org/10.1038/s41598-018-37936-2
  79. 79. Aaruni PS. Management of pesticide residues in select spices [MSc dissertation]. Kerala Agricultural University; 2016.
  80. 80. Muralikrishna P, Mathew TB, Sreelakshmi P, Koshy BA, Paul A, Rajith R. Evaluation of different household practices to decontaminate organophosphate insecticide residues from Amaranthus tricolor L. Entomon. 2016;41(3):195–202. https://doi.org/10.33307/entomon.v41i3.181
  81. 81. Muralikrishna P, Mathew TB, Nithya PR, Paul A. Evaluation of different household practice to decontaminate synthetic pyrethroid insecticide residues from Amaranthus tricolor L. IJCS. 2019;7(5):2049–52.
  82. 82. Balkan T, Yılmaz O. Efficacy of some washing solutions for removal of pesticide residues in lettuce. Beni-Suef Univ J Basic Appl Sci. 2022;11(1):143. https://doi.org/10.1186/s43088-022-00324-x
  83. 83. Chen Q, Wang Y, Chen F, Zhang Y, Liao X. Chlorine dioxide treatment for the removal of pesticide residues on fresh lettuce and in aqueous solution. Food Control. 2014;40:106–12. https://doi.org/10.1016/j.foodcont.2013.11.035
  84. 84. Park DW, Yang YS, Lee YU, Han SJ, Kim HJ, Kim SH, et al. Pesticide residues and risk assessment from monitoring programmes in the largest production area of leafy vegetables in South Korea: a 15-year study. Foods. 2021;10(2):425. https://doi.org/10.3390/foods10020425

Downloads

Download data is not yet available.