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Plant Science Today (PST)

Research Articles

Vol. 13 No. sp2 (2026): Recent Advances in Agriculture

Integrated use of weed-derived biochar and inorganic fertilisers enhances growth, physiology and yield of wheat under rainfed subtropical conditions

DOI
https://doi.org/10.14719/pst.14053
Submitted
9 February 2026
Published
30-04-2026

Abstract

A field experiment was conducted during the rabi season of 2023–24 at the ACRA Research Farm, Jammu, to evaluate the potential of converting invasive weeds into biochar for improving wheat (Triticum aestivum L.) productivity under rainfed subtropical conditions. A total of 16 treatments including Lantana camara L. and Adhatoda vasica Nees biochar at 2, 4 and 6 t ha-¹ combined with 50 % or 100 % recommended dose of fertilisers (RDF), farmyard manure (FYM) treatments and an absolute control were evaluated in a randomized block design (RBD). Application of L. camara biochar at 6 t ha-¹ with 100 % RDF recorded the highest grain yield (4129.81 kg ha-¹), statistically at par with A. vasica biochar at 6 t ha-¹ with 100 % RDF (4081.95 kg ha-¹) and Lantana biochar at 6 t ha-¹ with 50 % RDF (4006.63 kg ha-¹). High-dose biochar treatments significantly improved plant height, tiller count, leaf area index, dry matter accumulation and SPAD chlorophyll values. Weed-derived biochar at 6 t ha-¹ is an effective strategy to manage invasive weeds while enhancing rainfed wheat yield, enabling a 50 % reduction in inorganic fertiliser use without yield penalty.

References

  1. 1. Food and Agriculture Organization of the United Nations. FAO cereal supply and demand brief. World Food Situation.
  2. 2. UkrAgroConsult. India’s wheat production estimated to hit record high of 115.3 million metric tonnes in 2024–25. 2025.
  3. 3. CEIC Data. India agricultural production: wheat: Jammu & Kashmir. 2024.
  4. 4. Sande TJ, Tindwa HJ, Alovisi AM, Shitindi MJ, Semoka JM. Enhancing sustainable crop production through integrated nutrient management: a focus on vermicompost, bio-enriched rock phosphate and inorganic fertilisers—a systematic review. Front Agron. 2024;6:1422876. https://doi.org/10.3389/fagro.2024.1422876
  5. 5. Sheoran S, Prakash D, Raj D, Mor VS, Yadav PK, Gupta RK, et al. Long-term organic and N fertilization influence the quality and productivity of pearl millet under pearl millet–wheat sequence in north India. Sci Rep. 2024;14:19503. https://doi.org/10.1038/s41598-024-70009-1
  6. 6. Ghosh T, Das B, Chakraborty D, Singh VK, Das D, Aggarwal P, et al. Evaluation of soil quality frameworks in rice–wheat systems under integrated nutrient management in Indo-Gangetic plains. Sci Rep. 2025;15:37511. https://doi.org/10.1038/s41598-025-21630-1
  7. 7. Paramesh V, Mohan Kumar R, Rajanna GA, Gowda S, Nath AJ, Madival Y, et al. Integrated nutrient management for improving crop yields, soil properties and reducing greenhouse gas emissions. Front Sustain Food Syst. 2023;7:1173258. https://doi.org/10.3389/fsufs.2023.1173258
  8. 8. ICAR–CRIDA. Central Research Institute for Dryland Agriculture [Internet]. 2026. https://www.icar-crida.res.in/
  9. 9. Pandian K, Vijayakumar S, Mustaffa MR, Subramanian P, Chitraputhirapillai S. Biochar-a sustainable soil conditioner for improving soil health, crop production and environment under changing climate: a review. Front Soil Sci. 2024;4:1376159. https://doi.org/10.3389/fsoil.2024.1376159
  10. 10. Pokharel U, Neelgund G, Ray RL, Balan V, Kumar S. Biochar for soil amendment: applications, benefits and environmental impacts. Bioengineering. 2025;12:1137. https://doi.org/10.3390/bioengineering12111137
  11. 11. Kracmarova-Farren M, Alexova E, Kodatova A, Mercl F, Szakova J, Tlustos P, et al. Biochar-induced changes in soil microbial communities: a comparison of two feedstocks and pyrolysis temperatures. Environ Microbiome. 2024;19:87. https://doi.org/10.1186/s40793-024-00631-z
  12. 12. Khan MA, Basir A, Fahad S, Adnan M, Saleem MH, Iqbal A, et al. Biochar optimizes wheat quality, yield and nitrogen acquisition in low fertile calcareous soil treated with organic and mineral nitrogen fertilizers. Front Plant Sci. 2022;13:879788. https://doi.org/10.3389/fpls.2022.879788
  13. 13. He D, Luo Y, Zhu B. Feedstock and pyrolysis temperature influence biochar properties and its interactions with soil substances: insights from a DFT calculation. Sci Total Environ. 2024;922:171259. https://doi.org/10.1016/j.scitotenv.2024.171259
  14. 14. Shahzadi A, Noreen Z, Alamery S, Zafar F, Haroon A, Rashid M, et al. Effects of biochar on growth and yield of Triticum aestivum L. under salt stress. Sci Rep. 2024;14:20024. https://doi.org/10.1038/s41598-024-70917-2
  15. 15. Choudhary R, Sharma SK, Dudwal BL, Meena S. From weed to feed: role of Lantana biochar in chickpea productivity.
  16. 16. Gupta S. WOCAT technology database [Internet]. 2023. https://qcat.wocat.net/en/wocat/technologies/view/technologies_6690/
  17. 17. Choudhary P, Prasad M, Choudhary M, Kumar A, Kumar S, Srinivasan R, et al. Exploring invasive weed biochar as soil amendment: a study on fodder oats productivity and soil biological properties. Environ Res. 2023;216:114527. https://doi.org/10.1016/j.envres.2022.114527
  18. 18. Gee GW, Bauder JW. Particle-size analysis. In: Methods of soil analysis. Part 1. Physical and mineralogical methods. 1986;5:383–411. https://doi.org/10.2136/sssabookser5.1.2ed.c15
  19. 19. Jackson ML. Soil chemical analysis. Englewood Cliffs (NJ): Prentice-Hall; 1973.
  20. 20. Walkley A, Black IA. An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci. 1934;37:29–38. https://doi.org/10.1097/00010694-193401000-00003
  21. 21. Subbiah BV, Asija GL. A rapid procedure for the estimation of available nitrogen in soils.
  22. 22. Olsen SR. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. Washington (DC): US Department of Agriculture; 1954.
  23. 23. Knudsen D, Peterson GA, Pratt PF. Lithium, sodium and potassium. In: Methods of soil analysis. Part 2. 1982;9:225–46. https://doi.org/10.2134/agronmonogr9.2.2ed.c13
  24. 24. Arnon DI. Copper enzymes in isolated chloroplasts. Plant Physiol. 1949;24:1. https://doi.org/10.1104/pp.24.1.1
  25. 25. Barrs HD, Weatherley PE. A re-examination of the relative turgidity technique for estimating water deficits in leaves. Aust J Biol Sci. 1962;15:413–28. https://doi.org/10.1071/BI9620413
  26. 26. Jabborova D, Jabbarov Z, Abdrakhmanov T, Fayzullaev O, Saharan BS, Perveen K, et al. Assessing the synergistic effects of biochar, hydrogel and biofertilizer on growth and physiological traits of wheat in saline environments. Funct Plant Biol. 2025;52:FP24277. https://doi.org/10.1071/FP24277
  27. 27. Ali A, Jabeen N, Chachar Z, Chachar S, Ahmed S, Ahmed N, et al. The role of biochar in enhancing soil health and interactions with rhizosphere properties and enzyme activities in organic fertilizer substitution. Front Plant Sci. 2025;16:1595208. https://doi.org/10.3389/fpls.2025.1595208
  28. 28. Omara P, Singh H, Singh K, Sharma L, Otim F, Obia A. Short-term effect of field application of biochar on cation exchange capacity, pH and electrical conductivity of sandy and clay loam soils. Technol Agron. 2023;3:1. https://doi.org/10.48130/TIA-2023-0016
  29. 29. Gu K, Gao K, Guan S, Zhao J, Yang L, Liu M, et al. The impact of combined application of biochar and organic fertilizer on growth and nutrient distribution in wheat under reduced chemical fertilizer conditions. Sci Rep. 2025;15:5285. https://doi.org/10.1038/s41598-025-88879-4
  30. 30. Shah M, Nasir M, Siddique AB, Hossain MS, Wan J. Synergistic effects of biochar and potassium co-application on growth, physiological attributes and antioxidant defense mechanisms of wheat under water deficit conditions. Plant Stress. 2024;12:100452. https://doi.org/10.1016/j.stress.2024.100452
  31. 31. Wang L, Liu S, Lu S, Lv T, Ma D, Kang G, et al. Irrigation and nitrogen fertilizer increase wheat productivity by improving root–shoot population coordination. Agric Water Manag. 2025;316:109589. https://doi.org/10.1016/j.agwat.2025.109589
  32. 32. Yu Z, Islam S, She M, Diepeveen D, Zhang Y, Tang G, et al. Wheat grain protein accumulation and polymerization mechanisms driven by nitrogen fertilization. Plant J. 2018;96:1160–77. https://doi.org/10.1111/tpj.14096
  33. 33. Hasan MM, Hasan MM, Teixeira da Silva JA, Li X. Regulation of phosphorus uptake and utilization: transitioning from current knowledge to practical strategies. Cell Mol Biol Lett. 2016;21:7. https://doi.org/10.1186/s11658-016-0008-y
  34. 34. Shumaila, Ullah S, Shah W, Hafeez A, Ali B, Khan S, et al. Biochar and seed priming technique with gallic acid: improving morpho-anatomical and physiological features of Solanum melongena L. under NaCl and boron stresses. ACS Omega. 2023;8:28207–32. https://doi.org/10.1021/acsomega.3c01720
  35. 35. Kavanová M, Lattanzi FA, Grimoldi AA, Schnyder H. Phosphorus deficiency decreases cell division and elongation in grass leaves. Plant Physiol. 2006;141:766–75. https://doi.org/10.1104/pp.106.079699
  36. 36. Zulfiqar B, Raza MA, Saleem MF, Aslam MU, Iqbal R, Muhammad F, et al. Biochar enhances wheat crop productivity by mitigating drought effects. PLoS One. 2022;17:e0267819. https://doi.org/10.1371/journal.pone.0267819
  37. 37. Garcia-Rodriguez AF, Moreno-Racero FJ, Álvarez R, Colmenero-Flores JM, Knicker H, Rosales MA. Biochar enhances nitrogen use efficiency in lettuce. Plant Soil. 2026;518:299–317. https://doi.org/10.1007/s11104-025-07997-0
  38. 38. Lihong W, Jianing G, Mahmood A, Rasheed A, Hassan MU, et al. Biochar: a promising amendment to mitigate drought stress in plants: review and future perspective. Not Bot Horti Agrobo. 2023;51:13447. https://doi.org/10.15835/nbha51413447
  39. 39. Boudjabi S, Ababsa N, Chenchouni H. Enhancing soil resilience and crop physiology with biochar application for mitigating drought stress in durum wheat (Triticum durum). Heliyon. 2023;9:e22909. https://doi.org/10.1016/j.heliyon.2023.e22909
  40. 40. Haider FU, Shakoor N, Zulfiqar U, Ahmed S, Tariq S, Li X. Synergistic effects of phenylalanine and biochar to ameliorate cadmium stress in maize (Zea mays). Ecotoxicol Environ Saf. 2025;302:118673. https://doi.org/10.1016/j.ecoenv.2025.118673
  41. 41. Gaurav S, Diptanu B, Eiji N, Kazuyuki I, Shigeto S, Sachiko H, et al. Impact of fresh and residual biochar on physiological attributes and grain quality of wheat (Triticum aestivum L.). J Appl Biol Biotechnol. 2025;13:135–45.
  42. 42. Ghaedi M, Bijanzadeh E, Behpouri A, Najafi-Ghiri M. Biochar application affected biochemical properties, yield and nutrient content of safflower under water stress. Sci Rep. 2024;14:20228. https://doi.org/10.1038/s41598-024-71131-w
  43. 43. Duan S, Al-Huqail AA, Alsudays IM, Younas M, Aslam A, Shahzad AN, et al. Effects of biochar types on seed germination and wheat growth under salt stress. BMC Plant Biol. 2024;24:487. https://doi.org/10.1186/s12870-024-05188-0
  44. 44. Yang Y, Ahmed W, Ye C, Yang L, Wu L, Dai Z, et al. Effect of biochar on flue-cured tobacco growth. Front Plant Sci. 2024;15:1225031. https://doi.org/10.3389/fpls.2024.1225031
  45. 45. Omokaro GO, Kornev KP, Nafula ZS, Chikukula AA, Osayogie OG, Efeni OS. Biochar for sustainable soil management: enhancing soil fertility and climate resilience. Farming Syst. 2025:100167. https://doi.org/10.1016/j.farsys.2025.100167
  46. 46. Noor H, Sun M, Algwaiz HI, Sher A, Fiaz S, Attia KA, et al. Chlorophyll fluorescence and grain filling in wheat under nitrogen application. Mol Biol Rep. 2022;49:7157–72. https://doi.org/10.1007/s11033-022-07612-w
  47. 47. Pyne S, Singh G, Chandra S, Bhatnagar A, Raverkar KP, Chaturvedi S, Roy A. Growth and productivity of wheat under different establishment methods.
  48. 48. Prieto P, Ochagavía H, Savin R, Griffiths S, Slafer GA. Dynamics of floret initiation and spike fertility in wheat. J Exp Bot. 2018;69:2633–45. https://doi.org/10.1093/jxb/ery105
  49. 49. Xu H, Hassan MA, Sun D, Wu Z, Jiang G, Liu B, et al. Effects of low temperature stress on wheat and phosphorus mitigation strategies. Front Plant Sci. 2022;13:807844. https://doi.org/10.3389/fpls.2022.807844
  50. 50. Kumar N, Intodia SK, Kuri R. Effect of biochar on wheat nutrient uptake under water stress. Environ Ecol. 2024;42:2053–7. https://doi.org/10.60151/envec/YPNP1463
  51. 51. Wilhelm WW. Dry-matter partitioning and leaf area of winter wheat. Soil Tillage Res. 1998;49:49–56. https://doi.org/10.1016/S0167-1987(98)00154-8
  52. 52. Rashid M, Hussain Q, Khan KS, Alvi S, Abro SA, Akmal M, et al. De-ashed biochar slow-release N fertilizer increased NUE in wheat and maize. Sci Rep. 2025;15:7748. https://doi.org/10.1038/s41598-025-90651-7
  53. 53. Singh S, Singh R, Singh K, Katoch K, Zaeen AA, Birhan DA, et al. Smart fertilizer technologies for sustainable agriculture. Smart Agric Technol. 2024;8:100504. https://doi.org/10.1016/j.atech.2024.100504
  54. 54. Yuan X, Gu X, Liang R, Ban G, Ma L, He T, et al. Combined application of biochar and nitrogen fertilizer in soils. Sci Total Environ. 2024;933:173160. https://doi.org/10.1016/j.scitotenv.2024.173160
  55. 55. Liu C, Wang C, Shang S, Ma J, Shan S, Yue Q, et al. Biochar-assisted agriculture. Plants. 2025;14:3273. https://doi.org/10.3390/plants14213273
  56. 56. Bolan S, Sharma S, Mukherjee S, Kumar M, Rao CS, Nataraj KC, et al. Biochar modulating soil biological health: a review. Sci Total Environ. 2024;914:169585. https://doi.org/10.1016/j.scitotenv.2023.169585
  57. 57. Kunnen K, Ali MM, Lataf A, Van Hees M, Nauts R, Horemans N, et al. Growth-stimulating potential of biochar in wheat soilless systems. Front Plant Sci. 2024;15:1414212. https://doi.org/10.3389/fpls.2024.1414212

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