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Plant Science Today (PST)

Research Articles

Vol. 13 No. sp2 (2026): Recent Advances in Agriculture

Multi-response optimisation of gas composition in modified atmosphere packaging for postharvest quality retention of button mushrooms (Agaricus bisporus)

DOI
https://doi.org/10.14719/pst.14126
Submitted
16 February 2026
Published
12-05-2026

Abstract

Button mushrooms (Agaricus bisporus) are highly susceptible to deterioration due to their rapid respiration rate, enzymatic browning and microbial load, which limit their shelf life. This study focused on optimising the gas composition of modified atmosphere packaging (MAP) to delay the physiological deterioration of button mushrooms using a simple lattice mixture design. The effects of oxygen (O2), carbon dioxide (CO2) and nitrogen (N2) at different combinations on colour (L*, a* and b*), firmness, physiological loss in weight (PLW), polyphenol oxidase (PPO) activity, antioxidant activity and microbial load were evaluated. Modified atmosphere packaging under optimised conditions significantly (p < 0.0001) maintained the post-harvest quality of button mushrooms compared with the control. The optimised condition (69 % O2, 10 % CO2 and 21 % N2) improved lightness by 7.5 % and firmness by 14.3 %, while reducing browning (a* by 22.0 %, b* by 8.5 %) and physiological weight loss by 93.5 %. The PPO activity decreased by 15.2 % and antioxidant activity increased by 17.7 %, indicating delayed senescence and oxidative deterioration. The quadratic Scheffe model revealed that all responses exhibited a strong correlation, showing a high R2 value (> 0.90) with a non-significant lack of fit. Numerical optimisation predicted an optimal gas composition of 69 % O2, 10 % CO2 and 21 % N2, with a desirability value of 0.725. Validation experiments closely matched predicted values, confirming that optimised MAP is an effective and practical approach for enhancing postharvest quality and extending the shelf life of button mushrooms.

References

  1. 1. NeysariFam V, Golmohammadi A, Tabatabaekoloor R, Tahmasebi M, Nematollahzadeh A, Razavi MS, et al. Preservation of button mushrooms using modified atmosphere and bio-nanocomposite film: A combined approach. Appl Food Res. 2025;101043. https://doi.org/10.1016/j.afres.2025.101043
  2. 2. Arjun AD, Chakraborty SK, Mahanti NK, Kotwaliwale N. Non-destructive assessment of quality parameters of white button mushrooms (Agaricus bisporus) using image processing techniques. J Food Sci Technol. 2022;59(5):2047–59. https://doi.org/10.1007/s13197-021-05219-w
  3. 3. Srivastava P, Kumar N, Bano R, Prakash P, Bunkar DS, Singh G, et al. Comparative evaluation of storage temperatures on physiological and microbiological parameters of chemically treated button mushroom (Agaricus bisporus) stored under modified atmospheric packaging (MAP). Asian J Dairy Food Res. 2025;44(3):435–41. https://doi.org/10.18805/ajdfr.DR-2153
  4. 4. Eslamizadeh A, Jari SK, Torkashvand AM, Farsi M, Salmi MS. Improving the nutritional value of button mushroom (Agaricus bisporus) through cornmeal supplementation in compost and peat substitution in casing soil. Future Foods. 2025;100849. https://doi.org/10.1016/j.fufo.2025.100849
  5. 5. Subrahmanyam K, Gul K, Sehrawat R, Allai FM. Impact of in-package cold plasma treatment on the physicochemical properties and shelf life of button mushrooms (Agaricus bisporus). Food Biosci. 2023;52:102425. https://doi.org/10.1016/j.fbio.2023.102425
  6. 6. Mitra S, Veerana M, Choi EH, Park G. Effects of pre-treatment using plasma on the antibacterial activity of mushroom surfaces. Foods. 2021;10(8):1888. https://doi.org/10.3390/foods10081888
  7. 7. Shonte TT, Grogan H, Frias Celayeta JM, Giordano FS, Reynolds A, O’Halloran O, et al. The effects of preharvest silicon treatment and passive MAP on quality and shelf life of white button mushrooms in thermoformed recycled PET packaging system. Coatings. 2024;14(6):754. https://doi.org/10.3390/coatings14060754
  8. 8. Yang Y, Jia O, Li Y, Feng B, Chang M, Meng J, et al. Effect of high CO₂ controlled atmosphere storage on postharvest quality of button mushroom (Agaricus bisporus). Foods. 2024;13(21):3486. https://doi.org/10.3390/foods13213486
  9. 9. Pogorzelska-Nowicka E, Hanula M, Wojtasik-Kalinowska I, Stelmasiak A, Zalewska M, Półtorak A, et al. Packaging in a high O₂ or air atmospheres and in microperforated films effects on quality of button mushrooms stored at room temperature. Agriculture. 2020;10(10):479. https://doi.org/10.3390/agriculture10100479
  10. 10. Suhaimi NH, Yeong YF, Jusoh N, Chew TL, Bustam MA, Mubashir M. RSM modeling and optimization of CO₂ separation from high CO₂ feed concentration over functionalized membrane. Polymers. 2022;14(7):1371. https://doi.org/10.3390/polym14071371
  11. 11. Teli SA, Sheikh AY, Shukla RM, Richa R, Faisal S, Kumar S, et al. Numerical optimization of drying of white button mushroom (Agaricus bisporus) employing microwave and fluidized bed drying for preparing value added product. J Agric Food Res. 2024;18:101360. https://doi.org/10.1016/j.jafr.2024.101360
  12. 12. Yan X, Meng F, Van TT, Tanaka F, Tanaka F. Impact of chitosan, trans-cinnamaldehyde, poly (vinyl alcohol) and TiO₂ bio-nanocomposites on preservation and flavor of postharvest button mushroom (Agaricus bisporus). LWT. 2025;217:117377. https://doi.org/10.1016/j.lwt.2025.117377
  13. 13. Zheng Y, Zhu Y, Zheng Y, Hu J, Chen J, Deng S. The effect of dielectric barrier discharge plasma gas and plasma-activated water on the physicochemical changes in button mushrooms (Agaricus bisporus). Foods. 2022;11(21):3504. https://doi.org/10.3390/foods11213504
  14. 14. Pourbagher R, Abbaspour-Fard MH, Khomeiri M, Sohbatzadeh F, Rohani A. Effects of gas type and cold plasma treatment time on Lecanicillium fungicola spores reduction and changes in qualitative, chemical and physiological characteristics of button mushroom during postharvest storage. J Food Process Preserv. 2022;46(10):e16901. https://doi.org/10.1111/jfpp.16901
  15. 15. Andrés A, Timón M, Molina G, González N, Petrón M. Effect of MAP storage on chemical, physical and sensory characteristics of níscalos (Lactarius deliciosus). Food Packag Shelf Life. 2014;1(2):179–89. https://doi.org/10.1016/j.fpsl.2014.02.001
  16. 16. Jin J, Deng X, Zhou J, Deng Y, Pan N, Luo Y, et al. The mechanisms of inactivation of polyphenol oxidase in fresh-cut Agaricus bisporus by dual-frequency ultrasound combined with electrolytic water. Ultrason Sonochem. 2025;114:107277. https://doi.org/10.1016/j.ultsonch.2025.107277
  17. 17. Mohapatra D, Bira ZM, Kerry JP, Frías JM, Rodrigues FA. Postharvest hardness and color evolution of white button mushrooms (Agaricus bisporus). J Food Sci. 2010;75(3):E146–52. https://doi.org/10.1111/j.1750-3841.2010.01518.x
  18. 18. Gholami R, Ahmadi E, Ahmadi S. Investigating the effect of chitosan, nanopackaging and modified atmosphere packaging on physical, chemical and mechanical properties of button mushroom during storage. Food Sci Nutr. 2020;8(1):224–36. https://doi.org/10.1002/fsn3.1294
  19. 19. Koushki M, Abras SK, Mohammadi M, Hadian Z, Poorfallah NB, Sharayei P, et al. Physicochemical properties of mushrooms as affected by modified atmosphere packaging and CaCl₂ dipping. Afr J Agric Res. 2011;6(24):5414–21. https://doi.org/10.5897/AJAR11.734
  20. 20. Dorostkar M, Moradinezhad F, Ansarifar E. Influence of active modified atmosphere packaging pre-treatment on shelf life and quality attributes of cold stored apricot fruit. Int J Fruit Sci. 2022;22(1):402–13. https://doi.org/10.1080/15538362.2022.2047137
  21. 21. Attri B, Srivastava A, Kamal S, Sharma V. Influence of packaging materials and size on shelf life and quality of white button mushroom during storage. Asian J Dairy Food Res. 2025;44(6). https://doi.org/10.18805/ajdfr.DR-2069
  22. 22. Guo Y, Jiang J, Pan Y, Yang X, Li H, Li H, et al. Effect of high O₂ treatments on physiochemical, lycopene and microstructural characteristics of cherry tomatoes during storage. J Food Process Preserv. 2019;43(11):e14216. https://doi.org/10.1111/jfpp.14216
  23. 23. Van de Velde F, Méndez-Galarraga MP, Grace MH, Fenoglio C, Lila MA, Pirovani MÉ. Changes due to high oxygen and high carbon dioxide atmospheres on the general quality and the polyphenolic profile of strawberries. Postharvest Biol Technol. 2019;148:49–57. https://doi.org/10.1016/j.postharvbio.2018.10.015
  24. 24. Wang X, He X, Wu X, Fan X, Wang F, Lin Q, et al. UV-C treatment inhibits browning, inactivates Pseudomonas tolaasii and reduces associated chemical and enzymatic changes of button mushrooms. J Sci Food Agric. 2022;102(8):3259–65. https://doi.org/10.1002/jsfa.11668
  25. 25. Salamat R, Ghassemzadeh HR, Ranjbar F, Jalali A, Mahajan P, Herppich WB, et al. The effect of additional packaging barrier, air movement and cooling rate on quality parameters of button mushroom (Agaricus bisporus). Food Packag Shelf Life. 2020;23:100448. https://doi.org/10.1016/j.fpsl.2019.100448
  26. 26. Li Y, Wang Y, Liu J, Yu K, Chen X, Yuan L, et al. Effect of modified atmosphere packaging on storage quality and lignification process of postharvest asparagus (Asparagus officinalis L.). Food Packag Shelf Life. 2025;51:101576. https://doi.org/10.1016/j.fpsl.2025.101576
  27. 27. Dai Y, Zhao X, Zuo J, Zheng Y. Effect of 100 % oxygen-modified atmosphere packaging on maintaining the quality of fresh-cut broccoli during refrigerated storage. Foods. 2023;12(7):1524. https://doi.org/10.3390/foods12071524
  28. 28. Kim KM, Ko JA, Lee JS, Park HJ, Hanna MA. Effect of modified atmosphere packaging on the shelf-life of coated, whole and sliced mushrooms. LWT-Food Sci Technol. 2006;39(4):365–72. https://doi.org/10.1016/j.lwt.2005.02.015
  29. 29. Srivastava P, Prakash P, Bunkar DS. Evaluation of modified atmospheric packaging (MAP), chemical treatments and low temperature on biochemical and textural attributes of button mushroom (Agaricus bisporus). Asian J Dairy Food Res. 2020;39(3):232–8. https://doi.org/10.18805/ajdfr.DR-1517

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