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Research Articles

Vol. 13 No. 3 (2026)

Synergistic effects of plant growth regulators and nano-nutrients on biochemical attributes of Ber cv. Apple Ber

DOI
https://doi.org/10.14719/pst.14234
Submitted
24 February 2026
Published
06-07-2026 — Updated on 15-07-2026
Versions

Abstract

Apple Ber is increasingly gaining commercial significance due to its nutritional value and the ability to cultivate in semi-arid conditions. However, limited information is available regarding the combined use of plant growth regulators (PGRs) and nanonutrients for improving the biochemical and nutraceutical quality of apple ber under semi-arid conditions. Combining PGRs and nanonutrients is a potential strategy to enhance the fruit metabolic efficiency and nutraceutical properties is the combination of PGRs and nanonutrients. The objective of the present study was to determine the potential of the combination of PGRs nanonutrients in influencing the biochemical parameters of Apple Ber. The experiment was conducted in a factorial randomised block design (RBD) with 3 replications involving five levels of growth regulators (Factor A) [Control (water spray), brassinosteroids (BRs) 30 ppm, BRs 40 ppm, MT 10 ppm and MT 15 ppm] and five levels of nanonutrients (Factor B) [Control (water spray), nano boric acid 0.08 % and 0.16 % and nano calcium nitrate 0.02 % and 0.05 %]. There were significant effects on total soluble solids, sugars, ascorbic acid, antioxidant activity, phenolics, flavonoids, carotenoids and acidity. Among the treatment combinations, MT (15 ppm) + nano boric acid (0.16 %) (A5 × B3) resulted in the highest performance by significantly enhancing carotenoids, phenolic content, antioxidant activity and total sugars. The study demonstrates the synergistic role of melatonin and nano-boron in enhancing secondary metabolites and antioxidant potential in Apple Ber fruits, which has been scarcely explored under Punjab semi-arid conditions. The findings provide a scientific basis for precise foliar nutrition enabled by nanotechnology to enhance the biochemical quality and nutraceutical value of fruits in semi-arid production systems.

References

  1. 1. Șumălan RL, Copolovici DM, Crișan M, Stănică F, Șumălan RM, Lupitu A, et al. Assessment of fruit traits and antioxidant capacity in wild and cultivated genotypes of Ziziphus sp. Plants. 2025;14(1):134. https://doi.org/10.3390/plants14010134
  2. 2. Singh L, Kaur H. Pre-harvest factors influencing the production and quality attributes in fruit crops. In: Wani WA, Rajan R, Ahmad F, editors. Temperate Nut Crops. Apple Academic Press; 2026. p. 377–406. https://doi.org/10.1201/9781779643513-11
  3. 3. Garrido-Auñón F, Puente-Moreno J, García-Pastor ME, Serrano M, Valero D. Brassinosteroids: An innovative compound family that could affect the growth, ripening, quality and postharvest storage of fleshy fruits. Plants. 2024;13(21):3082. https://doi.org/10.3390/plants13213082
  4. 4. Singh M, Goswami SP, Sachan P, Sahu DK, Beese S, Pandey SK. Nanotech for fertilizers and nutrients-improving nutrient use efficiency with nano-enabled fertilizers. J Exp Agric Int. 2024;46(5):220–47. DOI: https://doi.org/10.9734/jeai/2024/v46i52372
  5. 5. Haleema B, Shah ST, Basit A, Hikal WM, Arif M, Khan W, et al. Comparative effects of calcium, boron and zinc inhibiting physiological disorders, improving yield and quality of Solanum lycopersicum. Biology. 2024;13(10):766. https://doi.org/10.3390/biology13100766
  6. 6. Khandaker MM, Amirah FN, Majrashi A, Sajili MH, Mohd KS, Mat N. Peel colour, anthocyanin, TSS content and sensory evaluation of some common fruits: A comparative study. Aust J Crop Sci. 2018;12(11):1788–95. https://doi.org/10.3316/informit.096897155359226
  7. 7. Paul V, Singh A, Pandey R. Determination of titrable acidity (TA). In: Post-harvest Physiology of Fruits and Flowers. 2010. p. 44.
  8. 8. Horwitz W. Official and tentative methods of analysis of the Association of Official Agricultural Chemists. Washington (DC): AOAC; 1970.
  9. 9. Lane JH, Eynon L. Determination of reducing sugars by Fehling's solution with methylene blue indicator. London: N. Rodger; 1934.
  10. 10. Ismail BP. Ash content determination. In: Ismail BP, Nielsen SS, editors. Nielsen's Food Analysis Laboratory Manual. Cham: Springer International Publishing; 2024. p. 129–31. https://doi.org/10.1007/978-3-031-44970-3_14
  11. 11. Chandaka M. Review article on crude fiber. J Multidiscip Res. 2025;5(3):30–3. https://doi.org/10.37022/tjmdr.v5i3.786
  12. 12. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1):44–84. https://doi.org/10.1016/j.biocel.2006.07.001
  13. 13. Dulf FV, Vodnar DC, Dulf EH, Tosa MI. Total phenolic contents, antioxidant activities and lipid fractions from berry pomaces obtained by solid-state fermentation of two Sambucus species with Aspergillus niger. J Agric Food Chem. 2015;63(13):3489–500. https://doi.org/10.1021/acs.jafc.5b00520
  14. 14. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999;64(4):555–9. https://doi.org/10.1016/S0308-8146(98)00102-2
  15. 15. Curl AL, Bailey GF. Orange carotenoids. Part I: Comparison of carotenoids of Valencia orange peel and pulp. Part II: Carotenoids of aged canned Valencia orange juice. J Agric Food Chem. 1956;4(2):156–62. https://doi.org/10.1021/jf60060a007
  16. 16. Wang Y, Liu G, Zhang W, Li L, Yang W, Liu Y, et al. Melatonin treatment delayed fruit softening by regulating postharvest carbohydrate metabolism of Hami melon. Plant Physiol Biochem. 2025;219:109328. https://doi.org/10.1016/j.plaphy.2024.109328
  17. 17. Himanshu, Sharma S, Rana VS, Ankit, Thakur V, Kumar A, et al. Unlocking the sustainable role of melatonin in fruit production and stress tolerance: a review. CABI Agric Biosci. 2024;5(1):103. https://doi.org/10.1186/s43170-024-00309-z
  18. 18. Gaber EI, Hamed LM, El-Refaie MS, Samak RM, Habashy RN. Fostering sustainable potato production: enhancing quality and yield via potassium and boron applications. Int J Agric Nat Res. 2024;51(3):189–203. https://doi.org/10.7764/ijanr.v51i3.2581
  19. 19. Carrión-Antolí A, Martínez-Romero D, Guillén F, Zapata PJ, Serrano M, Valero D. Melatonin pre-harvest treatments leads to maintenance of sweet cherry quality during storage by increasing antioxidant systems. Front Plant Sci. 2022;13:863467. https://doi.org/10.3389/fpls.2022.863467
  20. 20. Zhao L, Yan S, Wang Y, Xu G, Zhao D. Evaluation of the effect of preharvest melatonin spraying on fruit quality of ‘Yuluxiang’ pear based on principal component analysis. Foods. 2023;12(18):3507. https://doi.org/10.3390/foods12183507
  21. 21. Liu Y, Feng Y, Chen S, Pan Y, Xu J, Yu W, et al. Revealing the significance of melatonin in postharvest quality of tomato fruit, especially in sugar metabolism and transport. Postharvest Biol Technol. 2026;231:113909. https://doi.org/10.1016/j.postharvbio.2025.113909
  22. 22. Zhou K, Cheng Q, Dai J, Liu Y, Liu Q, Li R, et al. Effects of exogenous melatonin on sugar and organic acid metabolism in early-ripening peach fruits. PLoS One. 2023;18(10):e0292959. https://doi.org/10.1371/journal.pone.0292959
  23. 23. Xiao Y, Wu Y, Huang Z, Guo M, Zhang L, Luo X, et al. Mechanism of induced soluble sugar accumulation and organic acid reduction in plum fruits by application of melatonin. BMC Plant Biol. 2024;24(1):1208. https://doi.org/10.1186/s12870-024-05949-x
  24. 24. El-Mogy MM, Ludlow RA, Roberts C, Müller CT, Rogers HJ. Postharvest exogenous melatonin treatment of strawberry reduces postharvest spoilage but affects components of the aroma profile. J Berry Res. 2019;9(2):297–307. https://doi.org/10.3233/JBR-180361
  25. 25. Madebo MP, Zheng Y, Jin P. Melatonin-mediated postharvest quality and antioxidant properties of fresh fruits: a comprehensive meta-analysis. Compr Rev Food Sci Food Saf. 2022;21(4):3205–26. https://doi.org/10.1111/1541-4337.12961
  26. 26. Medina-Santamarina J, Guillén F, Ilea MI, Ruiz-Aracil MC, Valero D, Castillo S, et al. Melatonin treatments reduce chilling injury and delay ripening, leading to maintenance of quality in cherimoya fruit. Int J Mol Sci. 2023;24(4):3787. https://doi.org/10.3390/ijms24043787
  27. 27. Himanshu, Jahirbhai KJ, Saxena D. Integrating plant growth regulators (PGRs) and nano-nutrients to alleviate fruit drop and enhance reproductive efficiency in ber cv. Apple Ber. J Appl Nat Sci. 2026;18(1):558–65. https://doi.org/10.31018/jans.v18i1.7369
  28. 28. Okatan V, Nafiye UN, Kalkan P, Urfali M, Kalkan H, Selvi T, et al. Effects of preharvest melatonin and boric acid applications on the pomological and biochemical characteristics of highbush blueberry (Vaccinium corymbosum cv. ‘Camellia’). Not Bot Horti Agrobot Cluj Napoca. 2025;53(4):14837. https://doi.org/10.15835/nbha53414837
  29. 29. Bal E. Physicochemical changes in ‘Santa Rosa’ plum fruit treated with melatonin during cold storage. J Food Meas Charact. 2019;13(3):1713–20. https://doi.org/10.1007/s11694-019-00088-6
  30. 30. El-Beltagi HS, Eshak NS, Mohamed HI, Bendary ES, Danial AW. Physical characteristics, mineral content and antioxidant and antibacterial activities of Punica granatum or Citrus sinensis peel extracts and their applications to improve cake quality. Plants. 2022;11(13):1740. https://doi.org/10.3390/plants11131740
  31. 31. Gurjar PS, Berwal MK, Kumar R, Sarolia DK, Choudhary MK. Post-harvest melatonin treatment maintains freshness and delayed peel browning by sustaining higher antioxidants in Indian jujube (Ziziphus mauritiana Lamk.) fruits. Erwerbs-Obstbau. 2025;67(1):21. https://doi.org/10.1007/s10341-024-01244-z
  32. 32. Bantounou M, Plascevic J, Galley HF. Melatonin and related compounds: antioxidant and anti-inflammatory actions. Antioxidants. 2022;11(3):532. https://doi.org/10.3390/antiox11030532
  33. 33. Zhang L, Yu Y, Chang L, Wang X, Zhang S. Melatonin enhanced the disease resistance by regulating reactive oxygen species metabolism in postharvest jujube fruit. J Food Process Preserv. 2022;46(3):e16363. https://doi.org/10.1111/jfpp.16363
  34. 34. Priyaxee B, Kavitha C, Sivakumar V, Senthil A, Johnson I, Padmadevi K. Preharvest melatonin application on enhancing quality and extending shelf life in papaya. Plant Sci Today. 2025;12(2). https://doi.org/10.14719/pst.6192
  35. 35. Yang C, Xu Y, Xie X, Wu Y, Gao Z, Li K, et al. Post-harvest physiology of vegetable crops and its regulation. In: Ahammed GJ, Zhou J, editors. Growth Regulation and Quality Improvement of Vegetable Crops: Physiological and Molecular Features. Singapore: Springer Nature Singapore; 2025. p. 495–557. https://doi.org/10.1007/978-981-96-0169-1_17
  36. 36. Padilla-González PA, Garrido-Auñón F, García-Pastor ME, Guillén F, Serrano M, Valero D, et al. Melatonin as a pre- and postharvest tool for enhancing fruit quality. Plants. 2026;15(2):331. https://doi.org/10.3390/plants15020331
  37. 37. Magri A, Petriccione M. Melatonin treatment reduces qualitative decay and improves antioxidant system in highbush blueberry fruit during cold storage. J Sci Food Agric. 2022;102(10):4229–37.https://doi.org/10.1002/jsfa.11774
  38. 38. Molla SM, Rastegar S, Omran VG, Khademi O. Ameliorative effect of melatonin against storage chilling injury in pomegranate husk and arils through promoting the antioxidant system. Sci Hortic. 2022;295:110889. https://doi.org/10.1016/j.scienta.2022.110889
  39. 39. Zhang H, Wang Y, Cheng J, Lei W, Xu K, Yin L, et al. Exogenous melatonin regulates hormone metabolism involved in delaying the ripening process in cherry tomato fruit during storage. Postharvest Biol Technol. 2025;230:113805. https://doi.org/10.1016/j.postharvbio.2025.113805
  40. 40. Kapoor L, Simkin AJ, George Priya Doss C, Siva R. Fruit ripening: dynamics and integrated analysis of carotenoids and anthocyanins. BMC Plant Biol. 2022;22(1):27. https://doi.org/10.1186/s12870-021-03411-w
  41. 41. Bhardwaj R, Pareek S, Mani S, Domínguez-Avila JA, González-Aguilar GA. A melatonin treatment delays postharvest senescence, maintains quality, reduces chilling injury and regulates antioxidant metabolism in mango fruit. J Food Qual. 2022;2022:2379556. https://doi.org/10.1155/2022/2379556
  42. 42. Singh Y, Thakur N, Meena NK. Studies on the effect of foliar spray of Zn, Cu and B on growth, yield and fruit quality of sweet orange (Citrus sinensis L.) cv. Mosambi. Int J Chem Stud. 2018;6(5):3260–64.
  43. 43. Abd El AE, Khalifa SM, Alqahtani MD, Abd-Alrazik AM, Abdel-Aziz H, Mancy A, et al. Nano-enhanced growth and resilience strategies for pomegranate cv. Wonderful: unveiling the impact of zinc and boron nanoparticles on fruit quality and abiotic stress management. J Agric Food Res. 2024;15:100908. https://doi.org/10.1016/j.jafr.2023.100908
  44. 44. Bons HK, Sharma A. Impact of foliar sprays of potassium, calcium and boron on fruit setting behavior, yield and quality attributes in fruit crops: a review. J Plant Nutr. 2023;46(13):3232–46. https://doi.org/10.1080/01904167.2023.2192242
  45. 45. Kaur H, Devi KS, Yadav R. Physiological significance of zinc for plants, animals and humans. In: Srivastava S, Kaur H, editors. Zinc in Soil-Plant Continuum. Singapore: Springer Nature Singapore; 2025. p. 33–62. https://doi.org/10.1007/978-981-96-4253-3_2
  46. 46. Raliya R, Saharan V, Dimkpa C, Biswas P. Nanofertilizer for precision and sustainable agriculture: current state and future perspectives. J Agric Food Chem. 2018;66(26):6487–503. https://doi.org/10.1021/acs.jafc.7b02178
  47. 47. Dimkpa CO, Bindraban PS. Nanofertilizers: new products for the industry? J Agric Food Chem. 2018;66(26):6462–73. https://doi.org/10.1021/acs.jafc.7b02150

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