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Plant Science Today (PST)

Research Articles

Vol. 13 No. sp5 (2026): Recent Advances in Agriculture

Deciphering the antifungal potential of giloy (Tinospora cordifolia (Willd.) Hook. f. & Thomson): GC-MS profiling of stem extract against Macrophomina phaseolina

DOI
https://doi.org/10.14719/pst.14267
Submitted
27 February 2026
Published
31-08-2026

Abstract

Macrophomina phaseolina, a necrotrophic fungus, causes multiple diseases in mungbean and other economically important crops worldwide. The pathogen remains in soil or crop residues for up to 3 years as microsclerotia. This study aims to identify alternatives to current conventional practices, which are limited and are associated with toxicity and resistance. Therefore, there is an urgent need to analyse the other eco-friendly alternatives also. The application of medicinal plant extracts has shown enormous antifungal potential against many sclerotial-forming phytopathogens. The present study tested five concentrations (10, 20, 30, 40 and 50 %) of nine different medicinal plant extracts against the per cent mycelial inhibition of M. phaseolina under invitro conditions. The results revealed that all the plant extracts showed significant mycelial inhibition at all concentrations compared to the control. The maximum per cent mycelial inhibition was recorded in giloy (70.5 %), followed by curry leaf (60.7 %), which was at par with eucalyptus (56.0 %), followed by lemon grass (50.8 %) and bhang (46.5 %) at 50 % concentration. The maximum level of total phenol (291 mg GAE/g) and flavonoid (179 mg QE/g) content was observed in giloy. The qualitative analysis of plant extracts indicates the presence of flavonoids, alkaloids, phenols and proteins. Gas chromatography–mass spectrometry (GC-MS) analysis of the giloy (Tinospora cordifolia (Willd.) Hook.f. & Thomson ) showed the presence of 32 phytochemical compounds, among which cyclopentadecanone was the predominant one with 28.45 % peak area followed by 2- bromododecane (25.93 %), palmitic acid, TMS derivative (10.78 %), 2-hexadeccen-1-ol,3,7,11,15-tetramethyl (5.04 %), 2-hexadecen-1-ol, 3,7,11,15-tetramethyl (5.04 %), tetracosane (4.88 %), hexanoic acid, 4-hexadecyl ester (4.12 %) and butylated hydroxytoluene (0.79 %). Some of these major compounds might be responsible for the antifungal properties of Tinospora cordifolia against M. phaseolina.  Under invivo conditions, all plant extracts significantly promote crop growth. The results depicted that the shoot and root length of mungbean varied from 45.6 to 51.1 cm and 20.6 to 24.7 cm, respectively, but the highest shoot and root length was recorded in giloy (51.1 cm and 24.7 cm), followed by curry leaf (50.2 cm and 24.1 cm). The plant extract also promotes the nodules formation, which ranges from 16.5 to 19.9 nodules/plant. Simultaneously, pod formation was greatly influenced and varied from 7.6 to 14.8 pods/plant. Both nodulations (19.9 nodules/plant) and pod formation (14.8 pods/plant) were greatly enhanced by giloy, followed by curry leaf. All the plant extracts showed a significant reduction in the disease incidence. Overall, the study signifies that the minimum per cent disease incidence (PDI %) was recorded in giloy (50.1 %), followed by curry leaf (52.5 %).

References

  1. 1. Pandey AK, Burlakoti RR, Rathore A, Nair RM. Morphological and molecular characterization of Macrophomina phaseolina isolated from three legume crops and evaluation of mungbean genotypes for resistance to dry root rot. Crop Prot. 2020;127:104962. https://doi.org/10.1016/j.cropro.2019.104962
  2. 2. Kaushik CD, Chand JN. Seedborne nature of Rhizoctonia bataticola causing leaf blight of mungbean. Indian J Microbiol Res. 1987;17(2):154–57.
  3. 3. Bashir M, Malik BA. Diseases of major pulse crops in Pakistan-a review. Int J Pest Manag. 1988;34(3):309–14. https://doi.org/10.1080/09670878809371262
  4. 4. Pandey AK, Yee M, Win M, Lwin HMM, Adapala G, Rathore A, et al. Identification of new sources of resistance to dry root rot caused by Macrophomina phaseolina isolates from India and Myanmar in a mungbean mini-core collection. Crop Prot. 2021;143:105569. https://doi.org/10.1016/j.cropro.2021.105569
  5. 5. Saleh AA, Ahmed HU, Todd TC, Travers SE, Zeller KA, Leslie JF, et al. Relatedness of Macrophomina phaseolina isolates from tallgrass prairie, maize, soybean and sorghum. Mol Ecol. 2010;19(1):79–91. https://doi.org/10.1111/j.1365-294X.2009.04433.x
  6. 6. Basandrai AK, Pandey AK, Somta P, Basandrai D. Macrophomina phaseolina–host interface: Insights into an emerging dry root rot pathogen of mungbean and urdbean and its mitigation strategies. Plant Pathol. 2021;70(6):1263–75. https://doi.org/10.1111/ppa.13378
  7. 7. Khan AN, Shair F, Malik K, Hayat Z, Khan MA, Hafeez FY, et al. Molecular identification and genetic characterization of Macrophomina phaseolina strains causing pathogenicity on sunflower and chickpea. Front Microbiol. 2017;8:1309. https://doi.org/10.3389/fmicb.2017.01309
  8. 8. Ajayi-Oyetunde OO, Bradley CA. Rhizoctonia solani: Taxonomy, population biology and management of rhizoctonia seedling disease of soybean. Plant Pathol. 2018;67(1):3–17. https://doi.org/10.1111/ppa.12733
  9. 9. Javaid A, Khan IH, Shoaib A. Management of charcoal rot of mungbean by two Trichoderma species and dry biomass of Coronopus didymus. Planta Daninha. 2018;36:e018177098. https://doi.org/10.1590/s0100-83582018360100124
  10. 10. Abushaala FA, Ben Ramadan AR, Fahej MAS. In vitro antifungal activity of some plant extracts against seed-borne pathogens. IOSR J Agric Vet Sci. 2017;10(4):49–57. https://doi.org/10.9790/2380-1004014957
  11. 11. Freiesleben S, Jäger A. Correlation between plant secondary metabolites and their antifungal mechanisms—a review. Med Aromat Plants. 2014;3(154):1–6.
  12. 12. Vaou N, Stavropoulou E, Voidarou C, Tsigalou C, Bezirtzoglou E. Towards advances in medicinal plant antimicrobial activity: A review study on challenges and future perspectives. Microorganisms. 2021;9(10):2041. https://doi.org/10.3390/microorganisms9102041
  13. 13. Tamboli FA, More HN, Khairmode SS, Patil DR, Tambare PD, Shinde AJ, et al. Importance of medicinal plants and herbs as an immunity booster for pandemic COVID-19. TJPLS J. 2021;8(1):1–9. https://doi.org/10.61280/tjpls.v8i1.65
  14. 14. Jayswal MG. A comprehensive review on Tinospora cordifolia (Giloy): The medicinal plant. Int J Multidiscip Res Dev. 2021;8(6):80–85.
  15. 15. Khan IH, Javaid A. Antifungal potential of Chenopodium quinoa root extract against Macrophomina phaseolina (Tassi) Goid. J Allelopath. 2023;58(1):14–20. https://doi.org/10.26651/allelo.j/2023-58-1-1420
  16. 16. Banaras S, Javaid A, Khan IH. Potential antifungal constituents of Sonchus oleraceous against Macrophomina phaseolina. Int J Agric Biol. 2020;24(5):1376–82. https://doi.org/10.17957/IJAB/15.1573
  17. 17. Aftab A, Yousaf Y, Javaid A, Riaz N, Younas A, Rashid M, et al. Antifungal activity of vegetative methanolic extracts of Nigella sativa against Fusarium oxysporum and Macrophomina phaseolina and its phytochemical profiling by GC-MS analysis. Int J Agric Biol. 2019;21(3):569–76. https://doi.org/10.17957/IJAB/15.0930
  18. 18. Hemalatha R, Thamizhvani T, Dhivya AJA, Joseph JE, Babu B, Chandrasekaran R. Active contour-based segmentation techniques for medical image analysis. Med Image Anal. 2018;4(17):2. https://doi.org/10.5772/intechopen.74576
  19. 19. Singh C. Modern techniques of raising field crops. New Delhi: Oxford & IBH Publishing Co.; 1988. p. 167–70.
  20. 20. Cheng X, Zhang L, Luo J, Yang S, Deng Y, Li J, et al. Two pathogenic fungi isolated from chalkbrood samples and honey bee viruses they carried. Front Microbiol. 2022;13:843842. https://doi.org/10.3389/fmicb.2022.843842
  21. 21. Nene YL, Thapliyal PN. Fungicides in plant disease control. New Delhi: Oxford & IBH Publishing House; 1982. p. 163.
  22. 22. Vincent JM. Distortion of fungal hyphae in the presence of certain inhibitors. Nature. 1947;159:850. https://doi.org/10.1038/159850b0
  23. 23. Mishra D, Chitara MK, Chaturvedi P. Study of phytochemicals, antioxidant activity and antimicrobial properties of Catharanthus roseus (L.) G. Don. Emergent Life Sci Res. 2022;8:75–79. https://doi.org/10.5772/intechopen.74576
  24. 24. Zieslin N, Ben Zaken R. Peroxidase activity and presence of phenolic substances in peduncles of rose flowers. Plant Physiol Biochem. 1993;31(3):333–39.
  25. 25. Arvouet-Grand A, Vennat B, Pourrat A, Legret P. Standardization of propolis extract and identification of principal constituents. J Pharm Belg. 1994;49:462–68.
  26. 26. Shibula K, Velavan S. Determination of phytocomponents in methanolic extract of Annona muricata leaf using GC-MS technique. Int J Pharmacogn Phytochem Res. 2015;7(6):1251–55.
  27. 27. Choudhary S, Choudhary AK, Sharma OP. Screening of mungbean (Vigna radiata) genotypes to identify source of resistance to dry root rot. J Food Legumes. 2011;24(2):117–19.
  28. 28. Harman GE, Chet I, Baker R. Trichoderma hamatum effects on seed and seedling disease induced in radish and pea by Pythium spp. or Rhizoctonia solani. Phytopathology. 1980;70(12):1167–72. https://doi.org/10.1094/Phyto-70-1167
  29. 29. Gomez KA, Gomez AA. Statistical procedures for agricultural research. New York: John Wiley & Sons; 1984. p. 680.
  30. 30. Panse VG, Sukhatme PV. Statistical methods for agricultural workers. New Delhi: ICAR; 1954. p. 156.
  31. 31. Babicki S, Arndt D, Marcu A, Liang Y, Grant JR, Maciejewski A, et al. Heatmapper: Web-enabled heat mapping for all. Nucleic Acids Res. 2016;44:147–53. https://doi.org/10.1093/nar/gkw419
  32. 32. Deshmukh MM, Vanitha S. In vitro evaluation of leaf extracts against Macrophomina phaseolina in mulberry through poisoned food technique. Res Biotica. 2021;3(2):121–23. https://doi.org/10.54083/ResBio/3.2.2021.121-123
  33. 33. Oladipo AD, Nwogwugwu JO, Osunlaja OA, Batcho AA, Alade OD, Abimbola BO. The in vitro inhibition of Macrophomina and Lasiodiplodia species using three medicinal plant extracts. Ethiop J Environ Stud Manag. 2021;14(4).
  34. 34. Fatima N, Iqbal A, Khursid R, Rizwan M. In vitro studies on the growth inhibiting potential of some botanical extracts against Macrophomina phaseolina. Plant Cell Environ. 2019;1(1):15–20. https://doi.org/10.33687/jpe.001.01.3476
  35. 35. Kumar S, Chaudhary BK. Potential of few fungicides and plant extracts for managing charcoal rot of soybean caused by Macrophomina phaseolina (Tassi) Goid. in Madhya Pradesh, India. J Appl Nat Sci. 2020;12(3):388–93. https://doi.org/10.31018/jans.v12i3.2335
  36. 36. Lakhran L, Ahir RR. In vivo evaluation of different fungicides, plant extracts, biocontrol agents and organic amendments for management of dry root rot of chickpea caused by Macrophomina phaseolina. Legume Res. 2020;43(1):140–45.
  37. 37. Lattanzio V, Lattanzio VM, Cardinali A. Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. In: Phytochemistry: Advances in Research. 2006. p. 23–67.
  38. 38. Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N. Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants. 2019;8(4):96. https://doi.org/10.3390/plants8040096
  39. 39. Hmamou A, Eloutassi N, Alshawwa SZ, Al Kamaly O, Kara M, Bendaoud A, et al. Total phenolic content and antioxidant and antimicrobial activities of Papaver rhoeas L. organ extracts growing in Taounate region, Morocco. Molecules. 2022;27(3):854. https://doi.org/10.3390/molecules27030854
  40. 40. Hafshejani SF, Lotfi S, Rezvannejad E, Mortazavi M, Riahi-Madvar A. Correlation between total phenolic and flavonoid contents and biological activities of 12 ethanolic extracts of Iranian propolis. Food Sci Nutr. 2023;11(7):4308–18. https://doi.org/10.1002/fsn3.3356
  41. 41. Rongai D, Pulcini P, Pesce B, Milano F. Antifungal activity of some botanical extracts on Fusarium oxysporum. Open Life Sci. 2015;10(1):233–41. https://doi.org/10.1515/biol-2015-0040
  42. 42. Carrillo-Lomelí DA, de Rodríguez DJ, Moo-Huchin VM, Ramón-Canul L, Rodríguez-García R, González-Morales S, et al. How does Flourensia microphylla extract affect polyphenolic composition, antioxidant capacity and antifungal activity? Ind Crops Prod. 2022;186:115248. https://doi.org/10.1016/j.indcrop.2022.115248
  43. 43. Francis M, Chacha M, Ndakidemi PA, Mbega E. Phytochemical analysis and in vitro antifungal evaluation of Jatropha curcas against late leaf spot disease on groundnut. Med Aromat Plants. 2021;3(11).
  44. 44. Link KP, Angell HR, Walker JC. The isolation of protocatechuic acid from pigmented onion scales and its significance in relation to disease resistance in onions. J Biol Chem. 1929;81(2):369–75. https://doi.org/10.1016/S0021-9258(18)83819-4
  45. 45. Walker JC, Stahmann MA. Chemical nature of disease resistance in plants. Annu Rev Plant Physiol. 1955;6(1):351–66. https://doi.org/10.1146/annurev.pp.06.060155.002031
  46. 46. Wilson CL, Wisniewski ME. Biological control of postharvest diseases of fruits and vegetables: An emerging technology. Annu Rev Phytopathol. 1989;27(1):425–41. https://doi.org/10.1146/annurev.py.27.090189.002233
  47. 47. Padmavati M, Sakthivel N, Thara KV, Reddy AR. Differential sensitivity of rice pathogens to growth inhibition by flavonoids. Phytochemistry. 1997;46(3):499–502. https://doi.org/10.1016/S0031-9422(97)00325-7
  48. 48. Weidenbörner M, Hindorf H, Jha HC, Tsotsonos P. Antifungal activity of flavonoids against storage fungi of the genus Aspergillus. Phytochemistry. 1990;29(4):1103–05. https://doi.org/10.1016/0031-9422(90)85412-9
  49. 49. Gopinath M, Bharathiraja B, Iyyappan J, Gnanasekaran R, Yuvaraj D, Dhithya V. Extracellular green synthesis of silver nanoparticles using extract of Mimosa pudica leaves and assessment of antibacterial and antifungal activity. Proc Natl Acad Sci India Sect B Biol Sci. 2020;90:1025–33. https://doi.org/10.1007/s40011-020-01175-1
  50. 50. Ma K, Kou J, Rahman MKU, Du W, Liang X, Wu F, et al. Palmitic acid mediated change of rhizosphere and alleviation of Fusarium wilt disease in watermelon. Saudi J Biol Sci. 2021;28(6):3616–23. https://doi.org/10.1016/j.sjbs.2021.03.040
  51. 51. Charlet R, Le Danvic C, Sendid B, Nagnan-Le Meillour P, Jawhara S. Oleic acid and palmitic acid from Bacteroides thetaiotaomicron and Lactobacillus johnsonii exhibit anti-inflammatory and antifungal properties. Microorganisms. 2022;10(9):1803. https://doi.org/10.3390/microorganisms10091803
  52. 52. Bhat MP, Nayaka S. Cave soil Streptomyces sp. strain YC69 antagonistic to chilli fungal pathogens exhibits in vitro anticancer activity against human cervical cancer cells. Appl Biochem Biotechnol. 2023:1–24. https://doi.org/10.1007/s12010-023-04388-y
  53. 53. Haruna A. GC-MS profiling and antifungal activities of Morinda citrifolia L. leaf extract against fungal pathogens of crown rot disease of banana. J Phytol. 2023;15:132–38. https://doi.org/10.25081/jp.2023.v15.8423
  54. 54. Awan ZA, Shoaib A, Schenk PM, Ahmad A, Alansi S, Paray BA. Antifungal potential of volatiles produced by Bacillus subtilis BS-01 against Alternaria solani in Solanum lycopersicum. Front Plant Sci. 2023;13:1089562. https://doi.org/10.3389/fpls.2022.1089562
  55. 55. Rafiq M, Javaid A, Kanwal A, Anwar A, Khan IH, Kanwal Q, et al. GC-MS analysis and antifungal potential of flower extract of Acacia nilotica subsp. indica against Macrophomina phaseolina. Microb Pathog. 2024;194:106819. https://doi.org/10.1016/j.micpath.2024.106819
  56. 56. Asghari A, Ghanbary T, Bakhshi M, Babaeizad V. Bioactive potential and GC-MS fingerprinting of extracts from endophytic fungi associated with seeds of some medicinal plants. Mycol Iran. 2023;10(1):55–67. https://doi.org/10.22092/MI.2023.360789.1242
  57. 57. Alsultan QMN, Sijam K, Rashid TS, Ahmad KB. GC-MS analysis and antibacterial activity of mangosteen leaf extracts against plant pathogenic bacteria. Am J Plant Sci. 2016;7(7):1013–20. https://doi.org/10.4236/ajps.2016.77096
  58. 58. Saini S, Raj K, Saini AK, Chugh RK, Lal M, Bhambhu MK. Efficacy of plant extracts in growth promotion and onion purple blotch management: Unveiling metabolite fingerprinting of promising neem leaf extracts through GC-MS. Eur J Plant Pathol. 2024;170(4):883–98. https://doi.org/10.1007/s10658-023-02810-z
  59. 59. Johnson M, Abugri DA. Occurrence, biochemical, antimicrobial and health effects of palmitic acid. Palmitic Acid. 2014;17(11):99–107.
  60. 60. Selim EM, Amer GA, Makhlouf AH. Management of Macrophomina phaseolina on tomato using some plant extracts, plant oils and some biocontrol agents. Plant Prot Pathol. 2021;12(9):631–38. https://doi.org/10.21608/jppp.2021.198995
  61. 61. Jang SJ, Kuk YI. Growth promotion effects of plant extracts on various leafy vegetable crops. Hortic Sci Technol. 2019;37(3):322–36. https://doi.org/10.7235/HORT.20190033
  62. 62. Sajeena A, John J, Sudha B, Meera AV, Karthika SR. Significance of botanicals for the management of plant diseases. In: Plant Health Under Biotic Stress. Vol. 1. Organic Strategies. 2019. p. 231–43. https://doi.org/10.1007/978-981-13-6043-5_12
  63. 63. Huang Y, Sun C, Guan X, Lian S, Li B, Wang C.Butylated hydroxytoluene induced resistance against Botryosphaeria dothidea in apple fruit. Front. Microbiol. 2021; 11, 599062. https://doi.org/10.3389/fmicb.2020.599062
  64. 64. Mahamuni SV. Antifungal properties of Burkholderia cenocepacia strain VIMP 01 (JQ867371) against Ceratocystis paradoxa and Alternaria alternata. Int J Bioassays. 2015;4:4290–5.

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