Medinilla speciosa Blume is an underutilised tropical fruit and a promising source of bioactive compounds. However, scientific data on its physicochemical characteristics remain limited. This study characterised the physicochemical properties of fresh M. speciosa fruit and determined the extraction yield. Preliminary analyses were performed on fresh M. speciosa fruit, including colour measurement (L*, a*, b* values), size assessment (horizontal and vertical diameters) and proximate composition analysis (moisture, ash, fat, protein and carbohydrate content), after which the fruit was subjected to ultrasound-assisted extraction (UAE) using methanol. The average fruit diameter ranged from 6.55–8.78 mm, while colour values indicated a reddish-purple hue. The colour development index (CDI) of M. speciosa fruit ranged from 0.904–0.972 and decreased as fruit size increased. Proximate analysis showed high moisture content (90.67 ± 0.43 %), relatively high ash (4.24 ± 0.18 %) and fat (3.95 ± 0.12 %) levels, with low protein and carbohydrate contents. Ultrasound-assisted extraction (UAE) using methanol yielded an extraction efficiency of 14.95 % from M. speciosa fruit, which was 8–10 % higher than the average yields reported for M. speciosa extracted with other solvents or methods on a dry-weight basis. These findings provide essential baseline data to support future research and industrial applications of M. speciosa fruit extract in functional foods and nutraceuticals.