Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Vol. 13 No. sp6 (2026): National Conference on “Harnessing Genetic Resources for Food Security: Innovations in Conservation and Utilization for Sustainable Crop Improvement in Northeast Region”

Mapping and characterisation of mandarin (Citrus reticulata Blanco) genotypes across Eastern India

DOI
https://doi.org/10.14719/pst.14425
Submitted
5 March 2026
Published
18-08-2026

Abstract

Citrus is one of the most significant fruit crops in the Rutaceae family. The Northeastern Himalayan region of India and the foothills of the Central and Western Himalayan regions, often referred to as the “treasure house of citrus germplasm” are rich sources of citrus genetic diversity. The present study aimed to assess the morphological and biochemical variabilities among different mandarin (Citrus reticulata Blanco) germplasms cultivated/grown in Eastern India.  Following ICAR-National Bureau of Plant Genetic Resources (NBPGR) descriptors, a comprehensive survey and exploratory collection were conducted from native populations across West Bengal, Sikkim and Nagaland during 2024–25, covering diverse altitudinal and agro-climatic conditions. Mandarins collected from Dzongu (North Sikkim) exhibited the highest fruit weight (112.98 g) and total soluble solids (TSS: 10.13 ± 1.08 °Brix), along with superior vitamin C content (33.48 ± 4.44 mg/100 mL) and TSS:Acid ratio (20.87 ± 3.97), suggesting superior sweetness and nutritional quality. In contrast, Tseminyü (Nagaland) recorded the lowest fruit weight (61.05 g) and highest titratable acidity (1.04 ± 0.08 %), while Rüsoma (Nagaland) showed the highest reducing sugar (4.67 ± 1.98 %) and large fruit size (fruit length 57.62 mm, width 63.38 mm). Overall, the study revealed considerable genetic variability among the collected accessions, identifying Dzongu and Rüsoma as promising sources for quality planting material and breeding programs aimed at fruit quality improvement in mandarin. High-quality planting material can be produced using the accession.

References

  1. 1. Mir RW, Sardawarti KR, Mirza A. Evaluation of citrus germplasm for physico-chemical and morphological traits. Int J Chem Stud. 2020;9(1):423–29. https://doi.org/10.22271/chemi.2021.v9.i1f.11264
  2. 2. Rana A, Singh S, Bakshi M, Singh SK. Studies on genetic variability, correlation, and path analysis for morphological, yield, and yield-attributed traits in okra (Abelmoschus esculentus L. Moench). Int J Agric Stat Sci. 2020;16(1):387–94.
  3. 3. Fatima BM, Usman S, Khan MS, Khan IA, Khan MM. Identification of citrus polyploids using chromosome counts, morphological and SSR markers. Pak J Agric Sci. 2015;52:107–14.
  4. 4. Ahmed S, Rattanpal HS, Singh G. Diversity assessment of grapefruit (Citrus × paradisi) and tangelo (Citrus × tangelo) under Indian conditions using physico-chemical parameters and SSR markers. Appl Ecol Environ Res. 2018;16:5343–58. https://doi.org/10.15666/aeer/1605_53435358
  5. 5. Liu YZ, Deng XX. Citrus breeding and genetics in China. Asian Australas J Plant Sci Biotechnol. 2007;5:23–28.
  6. 6. Sunaiana, Gupta M, Rattanpal HS, Sidhu GS, Singh G. Genetic diversity in sweet orange (Citrus sinensis Osbeck) germplasm based on fruit morphological and physiological traits. Res Crops. 2018;19:425–29.
  7. 7. Kaur H, Sidhu GS, Sarao NK, Singh R, Singh G. Assessment of genetic diversity of mandarin cultivars grown in major citrus regions of the world using morphological and microsatellite markers. Hortic Environ Biotechnol. 2022. https://doi.org/10.1007/s13580-021-00404-4
  8. 8. Lin KH, Lai YC, Chang KY, Cheng YF, Hwang SY, Lo HF. Improving breeding efficiency for quality and yield of sweet potato. Bot Stud. 2007;48:283–92.
  9. 9. Gaikwad KA, Patil SR, Nagree PK, Potdukhe NR. Morphological characterization of citrus rootstock genotypes. Int J Chem Stud. 2018;6:516–29.
  10. 10. Singh A, Singh G, Kalia A, Rattanpal HS, Gupta M. Leaf morphoanatomical diversity analysis in mandarin (Citrus reticulata Blanco) genotypes using scanning electron microscopy. Genet Resour Crop Evol. 2020;67:2173–94. https://doi.org/10.1007/s10722-020-00972-x
  11. 11. Singh J, Dhaliwal HS, Thakur A, Chhuneja P, Sidhu GS, Singh R. Morphological and genetic diversity in citrus genotypes to substantiate rootstock breeding for root rot resistance. Indian J Hortic. 2017;74:326–33. https://doi.org/10.5958/0974-0112.2017.00066.4
  12. 12. Rattanpal HS, Singh H, Uppal GS. Genetic divergence in trifoliate citrus rootstocks under sub-tropical conditions of Punjab. J Pharmacogn Phytochem. 2018;7:953–57.
  13. 13. Malik SK, Chaudhury R, Dhariwal OP, Kalia RK. Collection and characterization of Citrus indica Tanaka and C. macroptera Montr., wild endangered species of northeastern India. Genet Resour Crop Evol. 2006;53:1485–93. https://doi.org/10.1007/s10722-005-7468-7
  14. 14. Sharma BD, Hore DK, Gupta SG. Genetic resources of Citrus of north-eastern India and their potential use. Genet Resour Crop Evol. 2004;51:411–18. https://doi.org/10.1023/B:GRES.0000023456.70577.3d
  15. 15. Dugo G, Di Giacomo A. Citrus: The genus Citrus. Medicinal and Aromatic Plants – Industrial Profiles. London: Taylor and Francis Group; 2002. https://doi.org/10.1201/9780203216613
  16. 16. Kumar S, Jena SN, Nair NK. ISSR polymorphism in Indian wild orange (Citrus indica Tanaka, Rutaceae) and related wild species in North-East India. Sci Hortic. 2010;123:350–59. https://doi.org/10.1016/j.scienta.2009.10.008
  17. 17. Ngachan SV, Roy SS, Sharma PK, Patel RK, Prakash N. Citrus scenario in North Eastern India: issues and strategies. In: Shivankar VJ, Singh IP, editors. Citrus Biodiversity: National Seminar on Citrus Biodiversity for Livelihood and Nutritional Security; 2010 Oct 4–5; Nagpur, India. p. 28–37.
  18. 18. Hangsing H, Mathew B, Kalita DC. Performance of Khasi Mandarin in Garo Hills of Meghalaya. Int J Sci Environ Technol. 2016;5(5):3213–23.
  19. 19. Barbora AC, Saikia J, Kakoti RK, Deka S, Hazarika B, Gogoi A, et al. Survey, selection and evaluation of elite Khasi mandarin (Citrus reticulata Blanco) genotypes for growth, yield, quality and biotic stress tolerance under climatic conditions of North-Eastern region. Int J Pure Appl Biosci. 2019;7(1):469–74. https://doi.org/10.18782/2320-7051.7483
  20. 20. Neves CG, Do Amaral DOJ, De Paula MFB, De Nascimento LS, Costantino G, Passos OS, et al. Characterization of tropical mandarin collection: Implications for breeding related to fruit quality. Sci Hortic. 2018;239:289–99. https://doi.org/10.1016/j.scienta.2018.05.022
  21. 21. Li Y, Suontama M, Burdon RD, Gea L, Dungey HS. Genotype by environment interactions in forest tree breeding: Review of methodology and perspectives on research and application. Tree Genet Genomes. 2017;13:60. https://doi.org/10.1007/s11295-017-1144-x
  22. 22. Angidi S, Madankar K, Tehseen MM, Bhatla A. Advanced high-throughput phenotyping techniques for managing abiotic stress in agricultural crops—A comprehensive review. Crops. 2025;5(2):8. https://doi.org/10.3390/crops5020008
  23. 23. Ghosh SP. Citrus industry of Northeast India. Punjab Hortic J. 1977;17:13–21.
  24. 24. Ranganna S. Handbook of analysis and quality control for fruit and vegetable products. New Delhi: Tata McGraw-Hill Publishing Company; 1986.
  25. 25. AOAC. Official methods of analysis. Assoc Off Anal Chem; 1995. Chapters 44, 45, Methods 44.2.08, 45.1.14. p. 16.
  26. 26. Jagota S, Dani HM. A new colorimetric technique for the estimation of vitamin C using Folin phenol reagent. Anal Biochem. 1982;127(1):178–82. https://doi.org/10.1016/0003-2697(82)90162-2
  27. 27. Hedge JE, Hofreiter BT. Methods in carbohydrate chemistry. In: Whistler RL, BeMiller JN, editors. Vol. 17. New York: Academic Press; 1962. p. 420.
  28. 28. Sheroan OP, Pannu RS. Statistical Package for Agricultural Workers. Hisar, India: O.P. Stat College of Agriculture, Kaul, CCS Haryana Agricultural University; 1999.
  29. 29. Savić S, Belić L, Marjanović M, Radović I, Girek Z, Zečević V, et al. Determination of bioactive components in different tomato lines: Physicochemical properties and antioxidant activity. Int Food Res J. 2024;31:87–97. https://doi.org/10.47836/ifrj.31.1.08
  30. 30. Abidi W, Moreno Sánchez MÁ, Gogorcena Aoiz Y. Phenotypic and biochemical diversity in peach [Prunus persica (L.) Batsch] cultivars. Sci Hortic. 2017;225:1–10. https://doi.org/10.1016/j.scienta.2017.07.012
  31. 31. Parekh B, Ahlawat TR, Patel AP, Jalandra AR, Limbachiya PV. Comparative biochemical profiling of fruits from thirty banana (Musa spp.) genotypes. Int J Biochem Res Rev. 2025;34(4):325–31. https://doi.org/10.9734/ijbcrr/2025/v34i41025
  32. 32. Ejjilani A, Houmanat K, Hanine H, Hssaini L, Elfazazi K, Hernandez F, et al. Pomegranate morpho-chemodiversity: Computational investigations based on in-vivo and in-vitro screening. Heliyon. 2022;8(5). https://doi.org/10.1016/j.heliyon.2022.e09345
  33. 33. Carli P, Arima S, Fogliano V, Tardella L, Frusciante L, Ercolano MR. Use of network analysis to capture key traits affecting tomato organoleptic quality. J Exp Bot. 2009;60(12):3379–86. https://doi.org/10.1093/jxb/erp177
  34. 34. González-López J, Rodríguez-Moar S, Silvar C. Correlation analysis of high-throughput fruit phenomics and biochemical profiles in native peppers (Capsicum spp.) from the primary center of diversification. Agronomy. 2021;11(2):262. https://doi.org/10.3390/agronomy11020262
  35. 35. Oh H, Mengist MF, Ma G, Giongo L, Pottorff M, Spencer JA, et al. Unraveling the genetic architecture of blueberry fruit quality traits: Major loci control organic acid content while more complex genetic mechanisms control texture and sugar content. BMC Plant Biol. 2025;25:36. https://doi.org/10.1186/s12870-025-06061-4
  36. 36. Debbabi OS, Bouhlal R, Abdelaali N, Mnasri S, Mars M. Pomological study of sweet orange (Citrus sinensis L. Osbeck) cultivars from Tunisia. Int J Fruit Sci. 2013;13:274–84. https://doi.org/10.1080/15538362.2012.679186
  37. 37. Malik SK, Rohini MR, Kumar S, Choudhary R, Pal D, Chaudhury R. Assessment of genetic diversity in sweet orange (Citrus sinensis (L.) Osbeck) cultivars of India using morphological and RAPD markers. Agric Res. 2012;1(4):317–24. https://doi.org/10.1007/s40003-012-0045-3
  38. 38. Susandarini R. Assessment of taxonomic affinity of Indonesian pummelo (Citrus maxima (Burm.) Merr.) based on morphological characters. Am J Agric Biol Sci. 2013;8(3):182–90. https://doi.org/10.3844/ajabssp.2013.182.190
  39. 39. Kar A, Debnath S, Rai M, Tyagi W, Tombisana Meetei N. Exploring altitude-driven diversity: Morphological, biochemical, and genetic diversity analysis of Khasi mandarin (Citrus reticulata Blanco) in Meghalaya, India. Genet Resour Crop Evol. 2025;72(5):5581–91. https://doi.org/10.1007/s10722-024-02287-7

Downloads

Download data is not yet available.