This study examines the organ-specific metabolic profiles of Myosotis alpestris F.W. Schmidt, focusing on soluble carbohydrates, free amino acids, water-soluble vitamins, flavonoids and mineral elements. Samples of roots, leaves and stem–flower tissues were collected from natural populations and analysed using high-performance liquid chromatography (HPLC) and multi-element analysis. The results showed that soluble carbohydrates, including glucose, fructose, sucrose and maltose, were unevenly distributed among plant organs, with the highest total carbohydrate content observed in stem–flower tissues. A total of 20 free amino acids were identified, with glutamine and cysteine
representing dominant components in aerial organs. Water-soluble vitamins (B1, B2, B3, B6, B9 and vitamin C) exhibited organ-specific accumulation patterns, with folic acid (B9) concentrated in roots and ascorbic acid in reproductive tissues. Flavonoid analysis revealed a more complex phenolic profile in aerial organs compared with roots. Elemental analysis demonstrated significant variation in macro-, micro- and trace element accumulation among plant organs, with calcium (Ca) and iron (Fe) predominantly enriched in stem–flower tissues. Overall, the findings indicate pronounced metabolic compartmentalisation in M. alpestris and demonstrate its potential as a valuable source of biologically active compounds. These results provide a foundation for future studies on the pharmacological, nutritional and ecological significance of this species.