Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Vol. 13 No. 3 (2026)

Identification of inbred lines derived from diverse cytoplasmic male sterile sources for female seed producibility in sunflower (Helianthus annuus L.)

DOI
https://doi.org/10.14719/pst.14626
Submitted
21 March 2026
Published
04-06-2026 — Updated on 10-08-2026
Versions

Abstract

Sunflower (Helianthus annuus L.) is an important oilseed crop known for its high oil content and wide adaptability. The present study was conducted to evaluate the seed productivity of cytoplasmic male sterile (CMS) lines and to identify promising female parents for hybrid development. The experiment was carried out during rabi 2024 at the All-India Coordinated Research Project on Sunflower, Zonal Agricultural Research Station, UAS, GKVK, Bengaluru. The experimental material consisted of 87 CMS lines representing diverse cytoplasmic sources and two male testers, GKVK-756 and GKVK-763. All CMS lines were crossed with the testers in a Line × Tester mating design, resulting in 174 hybrids. Data were recorded for head diameter (cm), seed yield (kg/ha) and oil content (%). Considerable variability was observed among the crosses for all the studied traits. Seed yield varied from 339 to 1558 kg/ha with GKVK-756 and 225 to 1662 kg/ha with GKVK-763. The highest seed yield was recorded in BCB116 × GKVK-763 (1662 kg/ha), while the lowest was observed in CMS234A × GKVK-763 (225 kg/ha). Based on average seed yield, the female lines BCB116, BCB82, BCB13, BCGB18 and BCB75 showed superior performance. The results confirmed significant genetic variability among CMS lines, enabling effective selection. Overall, the study identified 32 high-performing CMS lines representing diverse cytoplasmic sources for further hybrid evaluation, for integration into sunflower breeding programs for developing high-yielding hybrids with improved oil content.

References

  1. 1. Virupakshappa K, Nehru SD, Gowda J, Hegde S. Selection of testers for combining ability analysis and relationship between per se performance and GCA in sunflower. Helia. 1997;2(26):79–88.
  2. 2. Anonymous. Agricultural statistics at a glance. New Delhi: Directorate of Economics and Statistics; 2025.
  3. 3. Patil TRGM, Kulkarni VV, Kenganal M, Shankergoud I, Diwan JR. Combining ability studies in restorer lines of sunflower (Helianthus annuus L.). J Appl Nat Sci. 2017;9(1):603–8. https://doi.org/10.31018/jans.v9i1.1238
  4. 4. Leclercq P. Line sterile cytoplasmique chez tournesol. Ann Amelior Planta. 1969;12:99–106.
  5. 5. Kinman ML. New developments in USDA and state experiment station sunflower breeding programmes. In: Proceedings of the 4th International Sunflower Conference; 1970; Memphis, Tennessee. p.181–3.
  6. 6. Asif M, Shadakshari YG, Naik SJ, Venkatesha S, Vijayakumar KT, Basavaprabhu KV. Combining ability studies for seed yield and its contributing traits in sunflower (Helianthus annuus L.). Int Plant Sci. 2013;8(1):19–24.
  7. 7. Seetharam A. Hybrid sunflowers. Indian Farming. 1980;29 (12).
  8. 8. Kaul MLH. Male sterility in higher plants. Berlin: Springer; 1988. https://doi.org/10.1007/978-3-642-83139-3
  9. 9. Seetharam A. Hybrid seed production in sunflower (Helianthus annuus L.). In: Proceedings of National Seminar on Oilseeds; 1983.
  10. 10. Vasupalli N, Mogilicherla K, Shaik V, Rao KS, Bhat SR, Lin X. Advances in plant male sterility for hybrid seed production. Front Plant Sci. 2025;161540693. https://doi.org/10.3389/fpls.2025.1540693
  11. 11. Chakraborty NR, Lakshman SS, Debnath S, Rahimi M. Yield stability and economic heterosis analysis in newly bred sunflower hybrids throughout diverse agro-ecological zones. BMC Plant Biol. 2022;22:579. https://doi.org/10.1186/s12870-022-03983-1
  12. 12. Shelke PR, Pole SP, Dhuppe MV, Gund MS. Heterosis and its influence on seed yield and yield contributing traits in sunflower (Helianthus annuus L.) hybrids. Int J Adv Biochem Res. 2024;8(10):373–80. https://doi.org/10.33545/26174693.2024.v8.i10e.2522
  13. 13. Mijić A, Liović I, Zdunić Z, Marić S. Combining ability and heterosis in sunflower. Helia. 2009;32(50):163–72.
  14. 14. Radhika P, Jagadeshwar K, Khan KA. Combining ability studies in sunflower. Helia. 2012;35(56):47–56. https://doi.org/10.2298/HEL1257047F
  15. 15. Jadhao RB, Kharkar AP, Shaikh A, Sakhare SB. Combining ability studies for yield and its contributing characters in sunflower. Int J Adv Biochem Res. 2024;8(12):1012–6. https://doi.org/10.33545/26174693.2024.v8.i12m.3336
  16. 16. Kaya Y, Evci G, Durak S, Pekcan V, Gücer T. Determining relationships between yield and yield attributes in sunflower. Turk J Agric For. 2007;31:237–44.
  17. 17. Hussain F, Khan F, Ahmad J, Huo H, Jiang T, Rana I, et al. Sunflower (Helianthus annuus L.) hybrids: strategic crossbreeding techniques to efficiently enhance yield and oil quality. Phyton-Int J Exp Bot. 2025;94(10):3231–49. https://doi.org/10.32604/phyton.2025.069654
  18. 18. Singh RK, Chaudhary BD. Biometrical methods in quantitative genetic analysis. New Delhi: Kalyani Publishers; 1977.
  19. 19. Ramesh S, Shadakshari YG, Chandrashekara K. Combining ability in sunflower hybrids. Indian J Genet. 2006;66(2):105–8.
  20. 20. Doke SR, Naik GH, Ghodke MK. Study of combining ability and gene action in sunflower. Int J Res Agron. 2024;7(4):506–10. https://doi.org/10.33545/2618060X.2024.v7.i4g.598
  21. 21. Kumar AA, Ganesh M, Jeyakumar P. Heterosis studies in sunflower. Electron J Plant Breed. 2014;5(3):512–8.
  22. 22. Skoric D. Sunflower breeding for oil quality. Helia. 2009;32:1–16. https://doi.org/10.2298/HEL0950001S
  23. 23. Jan CC. Cytoplasmic male sterility in sunflower. Crop Sci. 2000;40:1485–90. https://doi.org/10.2135/cropsci2000.4061535x
  24. 24. Seiler GJ, Qi LL, Marek LF. Utilization of wild sunflower species for crop improvement. Crop Sci. 2017;57:1081–101. https://doi.org/10.2135/cropsci2016.10.0856
  25. 25. Singh G, Pole SP, Dhuppe MV, Zade PS, Narkhede TA. Heterosis studies for yield and oil content in sunflower. Agric J. 2025;7(9). https://doi.org/10.33545/2664844X.2025.v7.i9f.780
  26. 26. Ghaffari M, Farrokhi M, Mirzapour M. Genetic variability and trait association in sunflower. Afr J Biotechnol. 2013;12(7):689–95.
  27. 27. Tyagi V, Dhillon SK, Kaur G, Kaushik P. Heterotic effect of different cytoplasmic combinations in sunflower hybrids cultivated under diverse irrigation regimes. Plants. 2020;9:465. https://doi.org/10.3390/plants9040465

Downloads

Download data is not yet available.