Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Vol. 13 No. sp5 (2026): Recent Advances in Agriculture

Seed invigoration treatments as a tool for enhancing the vigour and performance of stored sorghum seeds

DOI
https://doi.org/10.14719/pst.15199
Submitted
24 April 2026
Published
10-09-2026

Abstract

Seed deterioration during storage adversely affects vigour and subsequent field performance of sorghum (Sorghum bicolor L. Moench), necessitating effective post-storage interventions to restore seed quality. The present study was conducted to evaluate the efficacy of different seed invigoration treatments in enhancing vigour, physiological performance, antioxidant activity and field establishment of stored sorghum seed lots differing in initial vigour status. Seeds subjected to various invigoration treatments were evaluated under laboratory and field conditions. The results indicated that all seed invigoration treatments improved germination, seedling vigour, biochemical attributes and field establishment compared with the untreated control, although the magnitude of improvement varied among treatments. Among the evaluated treatments, seed priming with BioNPK + drought alleviating bacteria (DAB) at 0.5 % (B6) significantly improved germination (83.83 %), mean germination time (33.30 hr), seedling vigour index I and II (1790 and 918 respectively) and improved antioxidant activity i.e. catalase, peroxidase, superoxide dismutase, proline content and reduced malondialdehyde content under laboratory conditions. Other priming treatments also exhibited positive effects but were comparatively less effective than B6. Under field conditions, all invigoration treatments improved field emergence, plant growth and yield-related traits over the untreated control, with BioNPK + DAB at 0.5 % (B6) outperforming other treatments by enhancing field emergence (71.78 %), plant growth parameters and yield parameters compared with the untreated control, with pronounced benefits observed in medium- and low-vigour seed lots. Enhanced early seedling establishment translated into improved crop growth and physiological performance under field conditions. The study demonstrates that appropriate seed invigoration treatments can effectively mitigate storage-induced deterioration, improve seed lot performance and serve as a practical tool for enhancing productivity in sorghum cultivation.

References

  1. 1. Winarti C, Arif AB, Budiyanto A, Richana N. Sorghum development for staple food and industrial raw materials in East Nusa Tenggara, Indonesia: a review. IOP Conf Ser Earth Environ Sci. 2020;443:012055. https://doi.org/10.1088/1755-1315/443/1/012055
  2. 2. Widowati S, Luna P. Nutritional and functional properties of sorghum (Sorghum bicolor (L.) Moench) based products and potential valorisation of sorghum bran. IOP Conf Ser Earth Environ Sci. 2022;1024:012031. https://doi.org/10.1088/1755-1315/1024/1/012031
  3. 3. ANGRAU. Sorghum outlook report June to May 2023–24. Guntur: Acharya N G Ranga Agricultural University; 2023.
  4. 4. Ebone LA, Caverzan A, Chavarria G. Physiologic alterations in orthodox seeds due to deterioration processes. Plant Physiol Biochem. 2019;145:34–42. https://doi.org/10.1016/j.plaphy.2019.10.028
  5. 5. Lamichhane JR, Debaeke P, Steinberg C, You MP, Barbetti MJ, Aubertot JN. Abiotic and biotic factors affecting crop seed germination and seedling emergence: a conceptual framework. Plant Soil. 2018;432(1):1–28. https://doi.org/10.1007/s11104-018-3780-9
  6. 6. Nawaz J, Hussain M, Jabbar A, Nadeem GA, Sajid M, Subtain M. Seed priming: a technique. Int J Agric Crop Sci. 2013;6(20):1373–81.
  7. 7. Dawood AA. Effect of seed treatment and seed size on seed vigor, field emergence and grain yield of Sorghum bicolor (L.) Moench. MSc Thesis. 2014:4.
  8. 8. Jatana BS, Grover S, Ram H, Baath GS. Seed priming: molecular and physiological mechanisms underlying biotic and abiotic stress tolerance. Agronomy. 2024;14(12):2901. https://doi.org/10.3390/agronomy14122901
  9. 9. Chakraborti S, Bera K, Sadhukhan S, Dutta P. Bio-priming of seeds: plant stress management and its underlying cellular, biochemical and molecular mechanisms. Plant Stress. 2022;3:100052. https://doi.org/10.1016/j.stress.2021.100052
  10. 10. Nawaz J, Hussain M, Jabbar A, Nadeem GA, Sajid M, Subtain MU, et al. Seed priming a technique. Int J Agric Crop Sci. 2013;6(20):1373.
  11. 11. Ali LG, Nulit R, Ibrahim MH, Yien CY. Efficacy of KNO3, SiO2 and SA priming for improving emergence, seedling growth and antioxidant enzymes of rice (Oryza sativa), under drought. Sci Rep. 2021;11(1):3864. https://doi.org/10.1038/s41598-021-83434-3
  12. 12. Rhaman MS, Imran S, Rauf F, Khatun M, Baskin CC, Murata Y, et al. Seed priming with phytohormones: an effective approach for the mitigation of abiotic stress. Plants. 2020;10(1):37. https://doi.org/10.3390/plants10010037
  13. 13. Saxena AK, Chakdar H, Kumar M, Rajawat MVS, Rajan. Microbe based technologies developed by ICAR. New Delhi: Indian Council of Agricultural Research; 2020.
  14. 14. Elumalai S, Alagarswamy S, Janaki P, Kuppusamy S, Geethanjali S, Parasuraman B. Exploring seaweed potential to enhance abiotic stress tolerance of crops. Russ J Plant Physiol. 2025;72(3):86. https://doi.org/10.1134/S1021443725600370
  15. 15. International Seed Testing Association. International rules for seed testing. Bassersdorf, Switzerland: International Seed Testing Association; 2024.
  16. 16. Abdul-Baki AA, Anderson JD. Vigor determination in soybean seed by multiple criteria. Crop Sci. 1973;13(6):630–3. https://doi.org/10.2135/cropsci1973.0011183X001300060013x
  17. 17. Pooja D, Sripathy KV, Siddaraju R, Bhojrajnaik K. Determination of mean germination time as an index of seed vigour in sorghum (Sorghum bicolor L.). J Adv Biol Biotechnol. 2025;28(10):1538–47. https://doi.org/10.9734/jabb/2025/v28i103168
  18. 18. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem. 1959;31(3):426–8. https://doi.org/10.1021/ac60147a030
  19. 19. Sadasivam S, Manickam A. Biochemical methods for agricultural science. New Delhi: Wiley Eastern Ltd; 1992. p. 246.
  20. 20. Goth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta. 1991;196(2–3):143–51. https://doi.org/10.1016/0009-8981(91)90067-M
  21. 21. Beauchamp C, Fridovich I. A mechanism for the production of ethylene from methional: the generation of the hydroxyl radical by xanthine oxidase. J Biol Chem. 1970;245(18):4641–6. https://doi.org/10.1016/S0021-9258(18)62842-X
  22. 22. Heath RL, Packer L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys. 1968;125(1):189–98. https://doi.org/10.1016/0003-9861(68)90654-1
  23. 23. Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil. 1973;39(1):205–7. https://doi.org/10.1007/BF00018060
  24. 24. Kata LP, Bhaskaran M, Umarani R. Influence of priming treatments on stress tolerance during seed germination of rice. Int J Agric Environ Biotechnol. 2014;7:225–32. https://doi.org/10.5958/2230-732X.2014.00238.1
  25. 25. Safiatou SS. Effect of different seed priming methods on germination, seedling establishment and vigour in sorghum (Sorghum bicolor (L.) Moench) and bambara groundnut (Vigna subterrenea (L.) Verdc.). Doctoral dissertation.
  26. 26. Shehzad M, Ayub M, Ahmad AHU, Yaseen M. Influence of priming techniques on emergence and seedling growth of forage sorghum. J Anim Plant Sci. 2012;22:154–8.
  27. 27. Meena RP, Sendhil R, Tripathi SC, Chander S, Chhokar RS, Sharma RK. Hydro-priming improves water use efficiency, grain yield and net returns of wheat. SAARC J Agric. 2013;11:149–59. https://doi.org/10.3329/sja.v11i2.18410
  28. 28. Agawane RB, Parhe SD. Effect of seed priming on crop growth and seed yield of soybean (Glycine max L.). Bioscan. 2015;10(1):265–70.
  29. 29. Sarika G, Basavaraju GV, Bhanuprakash K, Chaanakeshava V, Paramesh R, Radha BN. Seed viability and vigour of aged seed by priming in French bean. Veg Sci. 2013;40:169–73.
  30. 30. Narayanareddy AB, Biradarpatil NK. Influence of seed priming on storage of sunflower hybrid (KBSH-1). Bioinfolet. 2012;9:295–7.
  31. 31. El-Maarouf-Bouteau H. The seed and the metabolism regulation. Biology. 2022;11(2):168. https://doi.org/10.3390/biology11020168
  32. 32. Mahmood A, Turgay OC, Farooq M, Hayat R. Seed biopriming with plant growth promoting rhizobacteria: a review. FEMS Microbiol Ecol. 2016;92(8):fiw112. https://doi.org/10.1093/femsec/fiw112
  33. 33. Marthandan V, Geetha R, Kumutha K, Renganathan VG, Karthikeyan A, Ramalingam J. Seed priming: a feasible strategy to enhance drought tolerance in crop plants. Int J Mol Sci. 2020;21(21):8258. https://doi.org/10.3390/ijms21218258
  34. 34. Lara TS, Lira JMS, Rodrigues AC, Rakocevic M, Alvarenga AA. Potassium nitrate priming affects nitrate reductase and antioxidant enzymes in tomato germination. J Agric Sci. 2014;6:72–80. https://doi.org/10.5539/jas.v6n2p72
  35. 35. Sairam RK, Srivastava GC. Water stress tolerance of wheat: variations in hydrogen peroxide accumulation and antioxidant activity. J Agron Crop Sci. 2001;186:63–70. https://doi.org/10.1046/j.1439-037x.2001.00461.x
  36. 36. Chaparzadeh N, D'Amico ML, Khavari-Nejad RA, Izzo R, Navari-Izzo F. Antioxidative responses of Calendula officinalis under salinity conditions. Plant Physiol Biochem. 2004;42(9):695–701. https://doi.org/10.1016/j.plaphy.2004.07.001
  37. 37. Mohammadkhani N, Heidari R. Drought-induced accumulation of soluble sugars and proline in two maize varieties. World Appl Sci J. 2008;3(3):448–53.
  38. 38. Samota MK, Sasi M, Singh A. Impact of seed priming on proline content and antioxidant enzymes in rice genotype. Int J Curr Microbiol Appl Sci. 2017;6(5):2459–66. https://doi.org/10.20546/ijcmas.2017.605.275
  39. 39. Bayoumi TY, Eid MH, Metwali EM. Application of physiological and biochemical indices for drought tolerance in wheat. Afr J Biotechnol. 2008;7(14):2341–52.
  40. 40. Alalwani AK, Sekhi YS, Kamis A, Al-Dulaimy AFZ, Almohammedi OHM, Al-Taey DKA. Physiological activities of reactive oxygen species (ROS) in plants: a review. IOP Conf Ser Earth Environ Sci. 2025;1487:012063. https://doi.org/10.1088/1755-1315/1487/1/012063
  41. 41. Kumar RR, Karajol K, Naik GR. PEG-induced water stress on physiological and biochemical responses in pigeon pea. Recent Res Sci Technol. 2011;3(1):148–52.
  42. 42. Rehman H, Basra SMA, Farooq M. Field appraisal of seed priming to improve growth, yield and quality of direct seeded rice. Turk J Agric For. 2011;35(4):357–65. https://doi.org/10.3906/tar-1004-954
  43. 43. Tiwari TN, Dipti K, Singh RK, Prasad SR. Seed priming with potassium nitrate and water in pigeon pea. Ann Agric Res. 2014;35(2):164–70.
  44. 44. Shihab MO, Hamza JH. Seed priming of sorghum cultivars by gibberellic and salicylic acids under saline irrigation. J Plant Nutr. 2020;43(13):1951–67. https://doi.org/10.1080/01904167.2020.1766066
  45. 45. Farooq M, Basra SMA, Wahid A. Priming of field-sown rice seed enhances germination and yield. Plant Growth Regul. 2006;49:285–94. https://doi.org/10.1007/s10725-006-9138-y
  46. 46. Shoaei S, Noor-mohammadi G, Choukan R, Kashani A, Heydari SH, Rafiei F. Study of nutrient accumulation in the aerial and forage yield affected by using of nitroxin, supernitro plus and biophosphor in order to reduce consumption of chemical fertilizers and drought-resistant in corn (KSC-704). Adv Environ Biol. 2012;6(1):125–31.
  47. 47. Singh N, Thakur AK, Kaushal R, Mehta DK, Bhardwaj RK. Effects of seed pelleting on seed quality of cowpea during storage. Int J Econ Plants. 2018;5(2):76–9. https://doi.org/10.23910/IJEP/2018.5.2.0237
  48. 48. Basra SMA, Iftikhar MN, Afzal I. Potential of Moringa oleifera leaf extract as priming agent for hybrid maize seeds. Int J Agric Biol. 2011;13:1006–10.
  49. 49. Hussain M, Farooq M, Basra SMA, Ahmad N. Influence of seed priming techniques on sunflower. Int J Agric Biol. 2006;8:14–8.
  50. 50. Hajikhani S, Habibi H, Shekari F, Fotoukian MH. Effect of seed priming on grain yield of bean under water deficit stress. Iran J Field Crop Sci. 2011;42(1):191–7.
  51. 51. Ghassemi-Golezani K, Jabbarpour S, Zehtab-Salmasi S, Mohammadi A. Response of winter rapeseed cultivars to salt priming. Afr J Agric Res. 2010;5:1089–94.
  52. 52. Bakht J, Shafi M, Shah R. Effect of various priming sources on yield of maize cultivars. Pak J Bot. 2010;42(6):4123–31.

Downloads

Download data is not yet available.