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Plant Science Today (PST)

Research Articles

Early Access

Propagation of grapevine through air layering using rooting hormones

DOI
https://doi.org/10.14719/pst.15327
Submitted
29 April 2026
Published
03-10-2026
Versions

Abstract

Grapevine (Vitis vinifera L.) is an economically important fruit crop and the production of healthy, true-to-type planting material is a major constraint under conventional propagation methods due to poor rooting and low survival rates. Air layering, supplemented with suitable rooting hormones like IBA and NAA, can offer a promising alternative approach for rapid and efficient vegetative propagation. Keeping this in view, the present study was conducted at ICAR-National Research Centre for Grapes, Pune, Maharashtra, during 2022–23, to evaluate the influence of rooting hormones on air layering of Thompson Seedless grapevine grafted on Dogridge rootstock. The treatments consisted of IBA (2500 ppm), IBA (2500 ppm) + NAA (250 ppm), IBA (3000 ppm), IBA (3000 ppm) + NAA (250 ppm), along with an untreated control. Observations indicated that rooting hormones significantly improved air layering performance. The layers treated with 3000 ppm IBA exhibited a higher characteristic effect, viz., minimum number of days (56.00) taken for root initiation, maximum rooting percentage (78.33 %), length of the root (8.73 cm), diameter of the thickest root (3.28 mm), percentage of layers showing secondary roots (97.50 %), root dry matter (23.93 %), root volume (8.78 cm3), least number of days (15.25) required for the establishment of detached grafted layers, maximum percentage of final establishment of detached layers (94.67 %), number of new shoots per planted layer (6.83), length of shoot per planted layer (14.48 cm), length of the secondary shoot of layer (5.23 cm), layer girth (1.07 cm), leaf area (65.06 cm2), total carbohydrates (144.82 mg/g), total soluble protein (5.37 mg/g), peroxidase activity (0.133 mg/protein/min), polyphenol oxidase activity (0.790 EU/ml/min) and catalase activity (0.085 mg/protein/min). Thus, air layering supplemented with 3000 ppm IBA can provide a promising alternative for rapid vegetative propagation of grapevine.

References

  1. 1. National Horticulture Board. Area and production of horticulture crops: all India. Gurugram: National Horticulture Board, Government of India; 2022.
  2. 2. Hossain AM. Effect of time and variety on air layering in guava [MSc thesis]. Mymensingh: Agricultural University; 2007.
  3. 3. Vyas SV, Butani AM, Nurbhanej KH, Patel MS, Parmar LS. Effect of IBA and time of air layering on rooting and survival of air layers in red jamun (Syzygium cumini Skeels). Int J Pure Appl Biosci. 2016;5:272–9. https://doi.org/10.18782/2320-7051.5196
  4. 4. Hedge JE, Hofreiter BT. Carbohydrate chemistry. In: Whistler RL, BeMiller JN, editors. Methods in carbohydrate chemistry. New York: Academic Press; 1962. p. 17.
  5. 5. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265–75. https://doi.org/10.1016/S0021-9258(19)52451-6
  6. 6. Chance B, Maehly AC. Assay of catalase and peroxidase. Methods Enzymol. 1955;2:764–75. https://doi.org/10.1016/S0076-6879(55)02300-8
  7. 7. Waleed M. Extraction, purification and characterization of polyphenol oxidase from apricot, apple, eggplant and potato. Mesopotamia J Agric. 2009;37:28–36. https://doi.org/10.33899/magrj.2009.27497
  8. 8. Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121–6. https://doi.org/10.1016/S0076-6879(84)05016-3
  9. 9. Panse VS, Sukhatme PV. Statistical methods for agricultural workers. New Delhi: ICAR; 1989.
  10. 10. Bellini C, Pacurar DI, Perrone I. Adventitious roots and lateral roots: similarities and differences. Annu Rev Plant Biol. 2014;65:639–66. https://doi.org/10.1146/annurev-arplant-050213-035645
  11. 11. Druege U, Hilo A, Pérez-Pérez JM, Klopotek Y, Acosta M, Shahinnia F, et al. Molecular and physiological control of adventitious rooting in cuttings: phytohormone action meets resource allocation. Ann Bot. 2019;123(6):929–49. https://doi.org/10.1093/aob/mcy234
  12. 12. Rymbai H, Reddy GS. Effect of IBA, time of layering and rooting media on air layers and plantlets survival under different growing nursery conditions in guava. Indian J Hortic. 2010;67(4):99–104.
  13. 13. Rahman RAMN, Amin MS, Islam MM, Begum M, Uddin MA. Rooting and survival of litchi air layers. Pak J Biol Sci. 2002;5:1259–60. https://doi.org/10.3923/pjbs.2002.1259.1260
  14. 14. Denaxa NK, Vemmos SN, Roussos PA, Kostelenos G. The effect of IBA, NAA and carbohydrates on rooting capacity of leafy cuttings in three olive cultivars (Olea europaea L.). Acta Hortic. 2011;924:101–9. https://doi.org/10.17660/ActaHortic.2011.924.12
  15. 15. Ayesha S, Thippesha D. Influence of plant growth regulators on rooting of stem cuttings in dragon fruit [Hylocereus undatus (Haworth) Britton & Rose]. Int J Chem Stud. 2018;6(5):1834–9.
  16. 16. Singh AK, Singh R. Influence of growth regulating substances on rooting of cuttings of poinsettia cv. Flaming Sphere. Prog Hortic. 2005;37(1):85–8.
  17. 17. Tyagi SK, Patel RM. Effect of growth regulators on rooting of air layering of guava (Psidium guajava L.) cv. Sardar. Orissa J Hortic. 2004;32(1):58–62.
  18. 18. Patel KK. Effect of plant growth regulators on air layering of fig [MSc thesis]. Anand: Anand Agricultural University; 2010.
  19. 19. Tomar KS, Gurjar BS, Tomar RS. Response of different concentrations of IBA and NAA on rootage and growth of guava air layers (Psidium guajava L.) cv. Gwalior-27. Adv Plant Sci. 1999;12(2):535–8.
  20. 20. Kumar K, Syamal MM. Effect of etiolation and plant growth regulators on rooting in guava air layering. Indian J Hortic. 2005;62(3):290–2.
  21. 21. Baghel M, Raut UA, Ramteke VA. Effect of IBA concentrations and time of air layering in guava cv. L-49. Res J Agric Sci. 2016;7(1):117–20.
  22. 22. Singh KK, Choudhary T, Kumar A. Effect of various concentrations of IBA and NAA on rooting of stem cuttings of mulberry (Morus alba L.) under mist house condition in Garhwal hill region. Indian J Hill Farming. 2014;27(1):125–31.
  23. 23. Desale JV. Effect of IBA on air layering of karonda [MSc thesis]. Akola: Dr Panjabrao Deshmukh Krishi Vidyapeeth; 2011.
  24. 24. Haissig BE. Metabolic processes in adventitious rooting. In: Jackson MB, editor. New root formation in plants and cuttings. Dordrecht: Martinus Nijhoff; 1986. p. 141–89. https://doi.org/10.1007/978-94-009-4358-2_5
  25. 25. Da Costa CT, Almeida MR, Ruedell CM, Schwambach J, Maraschin FS, Fett-Neto AG. When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings. Front Plant Sci. 2013;4:133. https://doi.org/10.3389/fpls.2013.00133
  26. 26. Sato Y, Sugiyama M, Gorecki RJ, Fukuda H, Komamine A. Interrelationship between lignin deposition and peroxidase isoenzyme activities in differentiating tracheary elements of Zinnia. Planta. 1993;189:584–9. https://doi.org/10.1007/BF00198223
  27. 27. Liu ZH, Hsiao IC, Pan YW. Effect of NAA on auxin metabolism during rooting in soybean. Bot Bull Acad Sin. 1996;37:247–53.
  28. 28. Haissig BE. Metabolism during adventitious root primordium initiation and development. N Z J For Sci. 1974;4:324–37.
  29. 29. Bai T, Dong Z, Zheng X, Song S, Jiao J, Wang M, et al. Auxin and its interaction with ethylene control adventitious root formation and development in apple rootstock. Front Plant Sci. 2020;11:574881. https://doi.org/10.3389/fpls.2020.574881
  30. 30. Adem M, Sharma L, Shekhawat GS, Šafranek M, Jasik J. Auxin signaling, transportation and regulation during adventitious root formation. Curr Plant Biol. 2024;40:100385. https://doi.org/10.1016/j.cpb.2024.100385
  31. 31. Tyburski J, Krzemiński Ł, Tretyn A. The effect of auxin on catalase activity in tomato roots. Plant Growth Regul. 2008;54:203–15. https://doi.org/10.1007/s10725-007-9241-8
  32. 32. Ludwig-Müller J, Vertocnik A, Town CD. Analysis of indole-3-butyric acid-induced adventitious root formation on Arabidopsis stem segments. J Exp Bot. 2005;56(418):2095–105. https://doi.org/10.1093/jxb/eri208
  33. 33. Frick EM, Strader LC. Roles for IBA-derived auxin in plant development. J Exp Bot. 2018;69(2):169–77. https://doi.org/10.1093/jxb/erx298
  34. 34. Pop TI, Pamfil D, Bellini C. Auxin control in the formation of adventitious roots. Not Bot Horti Agrobot Cluj Napoca. 2011;39(1):307–16. https://doi.org/10.15835/nbha3916101

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