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Plant Science Today (PST)

Review Articles

Vol. 13 No. sp5 (2026): Recent Advances in Agriculture

Micronutrient enrichment of leguminous crops through agronomic biofortification: Strategies, challenges and future directions

DOI
https://doi.org/10.14719/pst.15686
Submitted
24 May 2026
Published
30-08-2026

Abstract

Hidden hunger refers to micronutrient deficiencies affecting nearly two billion people globally, especially in regions dependent on calorie-rich but nutrient-poor staple diets. Legumes, termed as the "poor man's meat" for their high protein content and low cost, are critical for enhancing nutritional security in resource-limited communities. This review emphasises agronomic biofortification as a rapid, promising and versatile strategy for enhancing essential micronutrients, particularly iron (Fe), zinc (Zn) and selenium (Se), in legume grains. It discusses different agronomic strategies such as soil and foliar fertilisation, seed priming and biological interventions. Unlike conventional breeding and genetic methods, which require longer to develop, these agronomic techniques can increase nutrient density within a single cropping cycle. It also focuses on the scope of agronomic biofortification and addresses its challenges related to nutritional bioavailability, notably the inhibitory effect of anti-nutritional factors such as phytates and discusses strategies to enhance nutrient absorption through promoter compounds. Furthermore, emerging technologies such as nanotechnology and precision agriculture are recognised as promising innovations to enhance nutrient use efficiency (NUE) and ensure environmental sustainability. Overall, agronomic biofortification provides a sustainable pathway to combat micronutrient malnutrition and this approach requires integration with genetic advancements and supportive agricultural policies for its long-term success.

References

  1. 1. Sheoran S, Kumar S, Ramtekey V, Kar P, Meena RS, Jangir CK. Current status and potential of biofortification to enhance crop nutritional quality: an overview. Sustainability. 2022;14(6):3301. https://doi.org/10.3390/su14063301
  2. 2. Tulchinsky TH. Micronutrient deficiency conditions: global health issues. Public Health Rev. 2010;32(1):243–55. https://doi.org/10.1007/BF03391600
  3. 3. Malik KA, Maqbool A. Transgenic crops for biofortification. Front Sustain Food Syst. 2020;4:571402. https://doi.org/10.3389/fsufs.2020.571402
  4. 4. Abbaspour N, Hurrell R, Kelishadi R. Review on iron and its importance for human health. J Res Med Sci. 2014;19(2):164–74.
  5. 5. Ofori KF, Antoniello S, English MM, Aryee ANA. Improving nutrition through biofortification–a systematic review. Front Nutr. 2022;9:1043655. https://doi.org/10.3389/fnut.2022.1043655
  6. 6. Roorkiwal M, Pandey S, Thavarajah D, Hemalatha R, Varshney RK. Molecular mechanisms and biochemical pathways for micronutrient acquisition and storage in legumes to support biofortification for nutritional security. Front Plant Sci. 2021;12:682842. https://doi.org/10.3389/fpls.2021.682842
  7. 7. Lakshmanan S, Rangasami SRS, Somasundaram S, Anantharaju P, Vijayalakshmi D, Rathika S, et al. Biofortification in pulses and legumes for alleviating malnutrition and enhancing nutritional security: a review. Appl Ecol Environ Res. 2025;23(1):889–914. https://doi.org/10.15666/aeer/2301_889914
  8. 8. Garousi F, Kovács B, Domokos-Szabolcsy É, Veres S. Biological changes of green pea (Pisum sativum L.) by selenium enrichment. Acta Biol Hung. 2017;68(1):60–72. https://doi.org/10.1556/018.68.2017.1.6
  9. 9. Dhaliwal SS, Sharma V, Shukla AK, Kaur J, Verma V, Singh P, et al. Enrichment of zinc and iron micronutrients in lentil (Lens culinaris Medik.) through biofortification. Molecules. 2021;26(24):7671. https://doi.org/10.3390/molecules26247671
  10. 10. Yadav P, Dhankhar S, Mehar R, Yadav V. Biofortification: a promising approach to enhance iron and zinc content in cowpea. Indian J Biochem Biophys. 2023;60(12):877–85. https://doi.org/10.56042/ijbb.v60i12.6415
  11. 11. Chasapis CT, Ntoupa PSA, Spiliopoulou CA, Stefanidou ME. Recent aspects of the effects of zinc on human health. Arch Toxicol. 2020;94(5):1443–60. https://doi.org/10.1007/s00204-020-02702-9
  12. 12. Podder R, Glahn RP, Vandenberg A. Iron- and zinc-fortified lentil (Lens culinaris Medik.) demonstrate enhanced and stable iron bioavailability after storage. Front Nutr. 2021;7:614812. https://doi.org/10.3389/fnut.2020.614812
  13. 13. Huang R, Bañuelos GS, Zhao J, Wang Z, Farooq MR, Yang Y, et al. Comprehensive evaluation of factors influencing selenium fertilization biofortification. J Sci Food Agric. 2024;104(10):6100–7. https://doi.org/10.1002/jsfa.13442
  14. 14. Jha AB, Warkentin TD. Biofortification of pulse crops: status and future perspectives. Plants. 2020;9(1):73. https://doi.org/10.3390/plants9010073
  15. 15. Vetési V, Záray G, Endrédi A, Sandil S, Rékási M, Takács T, et al. Iodine biofortification of bean (Phaseolus vulgaris L.) and pea (Pisum sativum L.) plants cultivated in three different soils. PLoS One. 2022;17(10):e0275589. https://doi.org/10.1371/journal.pone.0275589
  16. 16. Kaur S, Kumari A, Singh P, Kaur L, Sharma N, Garg M. Biofortification in pulses. In: Sharma TR, Deshmukh R, Sonah H, editors. Advances in agri-food biotechnology. Singapore: Springer; 2020. p. 85–103. https://doi.org/10.1007/978-981-15-2874-3_4
  17. 17. Mahto RK, Ambika, Singh C, Chandana BS, Singh RK, Verma S, et al. Chickpea biofortification for cytokinin dehydrogenase via genome editing to enhance abiotic-biotic stress tolerance and food security. Front Genet. 2022;13:900324. https://doi.org/10.3389/fgene.2022.900324
  18. 18. Gopalakrishnan S, Vadlamudi S, Samineni S, Kumar CVS. Plant growth-promotion and biofortification of chickpea and pigeon pea through inoculation of biocontrol potential bacteria, isolated from organic soils. SpringerPlus. 2016;5(1):1882. https://doi.org/10.1186/s40064-016-3590-6
  19. 19. Calles T. The international year of pulses: what are they and why are they important? Food and Agriculture Organization of the United Nations; 2016.
  20. 20. Tan GZH, Bhowmik SSD, Hoang TML, Karbaschi MR, Johnson AAT, Williams B, et al. Finger on the pulse: pumping iron into chickpea. Front Plant Sci. 2017;8:1755. https://doi.org/10.3389/fpls.2017.01755
  21. 21. Langyan S, Yadava P, Khan FN, Bhardwaj R, Bhardwaj R, Tripathi K, et al. Nutritional and food composition survey of major pulses toward healthy, sustainable and biofortified diets. Front Sustain Food Syst. 2022;6:878269. https://doi.org/10.3389/fsufs.2022.878269
  22. 22. Huertas R, Karpinska B, Ngala S, Mkandawire B, Maling’a J, Wajenkeche E, et al. Biofortification of common bean (Phaseolus vulgaris L.) with iron and zinc: achievements and challenges. Food Energy Secur. 2023;12(2):e406. https://doi.org/10.1002/fes3.406
  23. 23. White PJ, Broadley MR. Biofortification of crops with seven mineral elements often lacking in human diets—iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytol. 2009;182(1):49–84. https://doi.org/10.1111/j.1469-8137.2008.02738.x
  24. 24. Altaf MT, Liaqat W, Jamil A, Jan MF, Baloch FS, Barutçular C, et al. Strategies and bibliometric analysis of legumes biofortification to address malnutrition. Planta. 2024;260:85. https://doi.org/10.1007/s00425-024-04504-0
  25. 25. Jangir CK, Kumar S, Lakhran H, Meena RS. Towards mitigating malnutrition in pulses through biofortification. Trends Biosci. 2017;10(17):2999–3002.
  26. 26. Azeem A, Khan S, Haq MZU, Shafiq S, Aslam MT, Munir S, et al. Biofortification strategies for enhancing crop nutritional value: a review of methods, challenges and future directions. Discov Plants. 2025;2(1). https://doi.org/10.1007/s44372-025-00276-3
  27. 27. Bhardwaj AK, Chejara S, Malik K, Kumar R, Kumar A, Yadav RK. Agronomic biofortification of food crops: an emerging opportunity for global food and nutritional security. Front Plant Sci. 2022;13:1055278. https://doi.org/10.3389/fpls.2022.1055278
  28. 28. Kumar S, Pandey G. Biofortification of pulses and legumes to enhance nutrition. Heliyon. 2020;6(3):e03682. https://doi.org/10.1016/j.heliyon.2020.e03682
  29. 29. Kumar N, Meena RP, Kumar A, Hashim M, Deo MM, Nath CP. Recent innovations in nutrient management for enhancing productivity and nutritional quality in pulses and oilseeds. Indian J Fertilisers. 2025;21(11):1090–101.
  30. 30. Koul B, Sharma K, Sehgal V, Yadav D, Mishra M, Bharadwaj C. Chickpea (Cicer arietinum L.) biology and biotechnology: from domestication to biofortification and biopharming. Plants. 2022;11(21):2926. https://doi.org/10.3390/plants11212926
  31. 31. Padhy AK, Chaurasia S, Manivannan A, Tripathi K, Sapna S, Bhatia S. Innovations in industrial and functional food applications of lentil in the era of biofortification. Discov Food. 2025;5(1). https://doi.org/10.1007/s44187-025-00322-9
  32. 32. Shivay YS, Singh U, Prasad R, Kaur R. Agronomic interventions for micronutrient biofortification of pulses. Indian J Agron. 2016;61:161–72.
  33. 33. Jaiswal DK, Krishna R, Chouhan GK, De Araujo Pereira AP, Ade AB, Prakash S, et al. Bio-fortification of minerals in crops: current scenario and future prospects for sustainable agriculture and human health. Plant Growth Regul. 2022;98(1):5–22. https://doi.org/10.1007/s10725-022-00847-4
  34. 34. Ku YS, Rehman HM, Lam HM. Possible roles of rhizospheric and endophytic microbes to provide a safe and affordable means of crop biofortification. Agronomy. 2019;9(11):764. https://doi.org/10.3390/agronomy9110764
  35. 35. Singh U, Kumar N, Praharaj CS, Singh SS, Kumar L. Ferti-fortification: an easy approach for nutritional enrichment of chickpea. Ecoscan. 2015;9(3):731–6.
  36. 36. Ebbisa A. Mechanisms underlying cereal/legume intercropping as nature-based biofortification: a review. Food Prod Process Nutr. 2022;4(1). https://doi.org/10.1186/s43014-022-00096-y
  37. 37. Rehman A, Farooq M, Ullah A, Nadeem F, Im SY, Park SK, et al. Agronomic biofortification of zinc in Pakistan: status, benefits and constraints. Front Sustain Food Syst. 2020;4:591722. https://doi.org/10.3389/fsufs.2020.591722
  38. 38. Santi S, Schmidt W. Dissecting iron deficiency-induced proton extrusion in Arabidopsis roots. New Phytol. 2009;183(4):1072–84. https://doi.org/10.1111/j.1469-8137.2009.02908.x
  39. 39. Roriz M, Carvalho SMP, Castro PML, Vasconcelos MW. Legume biofortification and the role of plant growth-promoting bacteria in a sustainable agricultural era. Agronomy. 2020;10(3):435. https://doi.org/10.3390/agronomy10030435
  40. 40. Zuo Y, Zhang F. Iron and zinc biofortification strategies in dicot plants by intercropping with gramineous species: a review. Agron Sustain Dev. 2009;29(1):63–71. https://doi.org/10.1051/agro:2008055
  41. 41. Pal V, Singh G, Dhaliwal SS. A new approach in agronomic biofortification for improving zinc and iron content in chickpea (Cicer arietinum L.) grain with simultaneous foliar application of zinc sulphate, ferrous sulphate and urea. J Soil Sci Plant Nutr. 2021;21(2):883–96. https://doi.org/10.1007/s42729-021-00408-0
  42. 42. Avnee, Sood S, Chaudhary DR, Jhorar P, Rana RS. Biofortification: an approach to eradicate micronutrient deficiency. Front Nutr. 2023;10:1233070. https://doi.org/10.3389/fnut.2023.1233070
  43. 43. Petry N, Boy E, Wirth J, Hurrell R. Review: the potential of the common bean (Phaseolus vulgaris) as a vehicle for iron biofortification. Nutrients. 2015;7(2):1144–73. https://doi.org/10.3390/nu7021144
  44. 44. D’Amato R, Regni L, Falcinelli B, Mattioli S, Benincasa P, Bosco AD, et al. Current knowledge on selenium biofortification to improve the nutraceutical profile of food: a comprehensive review. J Agric Food Chem. 2020;68(14):4075–97. https://doi.org/10.1021/acs.jafc.0c00172
  45. 45. Kaur L, Sharma R, Singh G, Dhaliwal SS. Agronomic biofortification of mungbean [Vigna radiata (L.) Wilczek] grain with zinc to combat zinc malnutrition. J Soil Sci Plant Nutr. 2023;23(4):6206–15. https://doi.org/10.1007/s42729-023-01478-y
  46. 46. Minello LVP, Kuntzler SG, Berghahn E, Dorneles LT, Ricachenevsky FK, Sperotto RA. Nanotechnology-driven biofortification of Fe, Zn and Se in edible plants. J Nanobiotechnology. 2025;23(1):669. https://doi.org/10.1186/s12951-025-03746-8
  47. 47. Marra R, Lombardi N, Piccolo A, Bazghaleh N, Prashar P, Vandenberg A, et al. Mineral biofortification and growth stimulation of lentil plants inoculated with Trichoderma strains and metabolites. Microorganisms. 2021;10(1):87. https://doi.org/10.3390/microorganisms10010087
  48. 48. Singh T, Kothari M, Saini P, Singh AV, Verma AK, Singh BR, et al. Exploring diverse strategies for zinc biofortification: insights into enhancing crop zinc content. J Soil Sci Plant Nutr. 2025;25(3):5819–35. https://doi.org/10.1007/s42729-025-02499-5
  49. 49. Garg M, Sharma N, Sharma S, Kapoor P, Kumar A, Chunduri V, et al. Biofortified crops generated by breeding, agronomy and transgenic approaches are improving lives of millions of people around the world. Front Nutr. 2018;5:12. https://doi.org/10.3389/fnut.2018.00012
  50. 50. Lampkin N, Pearce B, Leake A, Creissen H, Gerrard CL, Gerling R, et al. The role of agroecology in sustainable intensification. 2015.
  51. 51. Duchene O, Vian JF, Celette F. Intercropping with legume for agroecological cropping systems: complementarity and facilitation processes and the importance of soil microorganisms. A review. Agric Ecosyst Environ. 2017;240:148–61. https://doi.org/10.1016/j.agee.2017.02.019
  52. 52. Li L, Tilman D, Lambers H, Zhang FS. Plant diversity and overyielding: insights from belowground facilitation of intercropping in agriculture. New Phytol. 2014;203(1):63–9.
  53. https://doi.org/10.1111/nph.12778
  54. 53. Hotegni NVF, Sohindji FS, Salaou MAB, Agbandou PC, Azonhoumon LWS, Tchokponhoué D, et al. Agronomic biofortification of cereals and legumes with iron, zinc, calcium and magnesium for food and nutrition security: available options for farmers in Sub-Saharan Africa. J Agric Food Res. 2024;18:101391. https://doi.org/10.1016/j.jafr.2024.101391
  55. 54. Kabir AH, Paltridge N, Stangoulis J. Chlorosis correction and agronomic biofortification in field peas through foliar application of iron fertilizers under Fe deficiency. J Plant Interact. 2016;11(1):1–4. https://doi.org/10.1080/17429145.2015.1125534
  56. 55. Guerrero-Martin CA, Ortega-Ramírez AT, Silva-Marrufo Ó, Casallas-Martín BD, Cortés-Salazar N, Salinas-Silva R, et al. Biofortification of kidney bean (Phaseolus vulgaris L.) crops applying zinc sulfate and ferric sulfate: pilot crop in Colombia. Molecules. 2023;28(5):2004. https://doi.org/10.3390/molecules28052004
  57. 56. Soriano LA, Silva ABP, Souza DHA, Oliveira CF, Neto AR, Silva GN, et al. Synergy between zinc fertilization and Azospirillum brasilense in cowpea biofortification and yield boost. J Soil Sci Plant Nutr. 2025;25(4):10541–55. https://doi.org/10.1007/s42729-025-02808-y
  58. 57. Farooq U, Akmal M, Ahmad Q, Akram Z, Arshad A, Qamar H, et al. Biofortification of mungbean (Vigna radiata) using iron-enriched organic amendment. Pak J Agric Res. 2021;34(3):394–9. https://doi.org/10.17582/journal.pjar/2021/34.3.394.399
  59. 58. Kshi S, Almad R, Rathod PS, Rachappa V, Dodamani BM, Ananda N. Growth, yield and economics of pigeonpea as influenced by biofortification of zinc and iron. Int J Curr Microbiol Appl Sci. 2020;9(2):3088–97. https://doi.org/10.20546/ijcmas.2020.902.356
  60. 59. Poblaciones MJ, Rodrigo SM, Santamaría O. Evaluation of the potential of peas (Pisum sativum L.) to be used in selenium biofortification programs under Mediterranean conditions. Biol Trace Elem Res. 2013;151(1):132–7. https://doi.org/10.1007/s12011-012-9539-x
  61. 60. Amaya-Olivas NI, Sánchez E, Hernández-Ochoa L, Ojeda-Barrios DL, Ávila-Quezada GD, Flores-Córdova MA, et al. Biofortification with magnesium nanofertilizer on bioactive compounds and antioxidant capacity in green beans. Not Bot Horti Agrobot Cluj Napoca. 2023;51(1):12830. https://doi.org/10.15835/nbha51112830
  62. 61. Ciscomani-Larios JP, Sánchez-Chávez E, Jacobo-Cuellar JL, Sáenz-Hidalgo HK, Orduño-Cruz N, Cruz-Alvarez O, et al. Biofortification efficiency with magnesium salts on the increase of bioactive compounds and antioxidant capacity in snap beans. Cienc Rural. 2021;51(6). https://doi.org/10.1590/0103-8478cr20200442
  63. 62. Boy E, Haas JD, Petry N, Cercamondi CI, Gahutu JB, Mehta S, et al. Efficacy of iron-biofortified crops. 2017.
  64. 63. Shahzad R, Jamil S, Ahmad S, Nisar A, Khan S, Amina Z, et al. Biofortification of cereals and pulses using new breeding techniques: current and future perspectives. Front Nutr. 2021;8:721728. https://doi.org/10.3389/fnut.2021.721728
  65. 64. Salaria S, Boatwright JL, Thavarajah P, Kumar S, Thavarajah D. Protein biofortification in lentils (Lens culinaris Medik.) toward human health. Front Plant Sci. 2022;13:869713. https://doi.org/10.3389/fpls.2022.869713
  66. 65. Rehman HM, Cooper JW, Lam HM, Yang SH. Legume biofortification is an underexploited strategy for combatting hidden hunger. Plant Cell Environ. 2019;42(1):52–70. https://doi.org/10.1111/pce.13368
  67. 66. Nair RM, Yang R, Easdown WJ, Thavarajah D, Thavarajah P, Hughes JD, et al. Biofortification of mungbean (Vigna radiata) as a whole food to enhance human health. J Sci Food Agric. 2013;93(8):1805–13. https://doi.org/10.1002/jsfa.6110
  68. 67. Coelho RC, Barsotti RCF, Maltez HF, Júnior CAL, De Sousa Barbosa H. Expanding information on the bioaccessibility and bioavailability of iron and zinc in biofortified cowpea seeds. Food Chem. 2021;347:129027. https://doi.org/10.1016/j.foodchem.2021.129027
  69. 68. Sodedji KAF, Assogbadjo AE, Lee B, Kim HY. An integrated approach for biofortification of carotenoids in cowpea for human nutrition and health. Plants. 2024;13(3):412. https://doi.org/10.3390/plants13030412
  70. 69. Bouis HE, Hotz C, McClafferty B, Meenakshi JV, Pfeiffer WH. Biofortification: a new tool to reduce micronutrient malnutrition. Food Nutr Bull. 2011;32(Suppl 1):S31–40. https://doi.org/10.1177/15648265110321S105
  71. 70. Sandhu R, Chaudhary N, Bindia, Shams R, Singh K, Pandey VK. A critical review on integrating biofortification in crops for sustainable agricultural development and nutritional security. J Agric Food Res. 2023;14:100830. https://doi.org/10.1016/j.jafr.2023.100830
  72. 71. Koç E, Karayiğit B. Assessment of biofortification approaches used to improve micronutrient-dense plants that are a sustainable solution to combat hidden hunger. J Soil Sci Plant Nutr. 2022;22(1):475–500. https://doi.org/10.1007/s42729-021-00663-1
  73. 72. Kapoor P, Dhaka RK, Sihag P, Mehla S, Sagwal V, Singh Y, et al. Nanotechnology-enabled biofortification strategies for micronutrients enrichment of food crops: current understanding and future scope. NanoImpact. 2022;26:100407. https://doi.org/10.1016/j.impact.2022.100407
  74. 73. Aqeel U, Aftab T, Khan MMA, Naeem M, Khan MN. A comprehensive review of impacts of diverse nanoparticles on growth, development and physiological adjustments in plants under changing environment. Chemosphere. 2022;291(Pt 1):132672. https://doi.org/10.1016/j.chemosphere.2021.132672
  75. 74. Windsor N, Boatwright L, Boyles R, Bridges W, Rubiales D, Thavarajah D. Characterizing dry pea (Pisum sativum L.) for improved nutritional traits and the potential for biofortification. Legume Sci. 2024;6(3). https://doi.org/10.1002/leg3.250
  76. 75. Venkatesh MS, Hazra KK, Ghosh PK, Singh KK. Improving productivity of maize-lentil rotation in alkaline Fluvisol following soil test crop response (STCR)-targeted yield approach of nutrient management. Arch Agron Soil Sci. 2022;68(7):929–43. https://doi.org/10.1080/03650340.2020.1864338

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