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Research Articles

Vol. 13 No. sp5 (2026): Recent Advances in Agriculture

Influence of phosphorus levels on phosphorus fractionation and transformation in Inceptisols

DOI
https://doi.org/10.14719/pst.15712
Submitted
26 May 2026
Published
31-08-2026

Abstract

Phosphorus (P), a crucial macronutrient for plant growth, is severely limited to Inceptisols due to its fixation by calcium (Ca), iron (Fe) and aluminium oxides (Al2O3), which renders it insoluble and unavailable for plant uptake. An experimental investigation involving soil incubation was conducted to examine the transformation of phosphorus (P) fractions in Inceptisol soil. Seven treatments were evaluated: absolute control, control and potassium dihydrogen phosphate (KH₂PO₄) at 20, 40, 60, 80 and 100 ppm. Soil samples were collected at 0, 10, 20, 30, 40 and 50 days of incubation. Phosphorus fractions and total P were determined using appropriate analytical methods, including the molybdenum blue spectrophotometric method for total P. Phosphorus fractions were significantly affected by both P concentration and incubation period. Soluble P initially increased and subsequently decreased, indicating its transformation into less-soluble forms. Stable P fractions, including calcium phosphate, iron-bound P and non-labile P gradually increased during the incubation period. Maximum P availability was observed on day 20. Principal component analysis (PCA) confirmed distinct clustering of the stable P fractions. Correlation analysis revealed strong positive relationships among the stable P fractions, whereas negative relationships were observed between soluble and stable P fractions. These findings indicate that the Inceptisol had a strong capacity to fix applied P into stable forms, suggesting that split or slow-release P fertilisation strategies, timed according to crop demand, may be necessary to sustain plant-available P and improve fertiliser-use efficiency in such soils.

References

  1. 1. Bowman RA. A sequential extraction procedure with concentrated sulfuric acid and dilute base for soil organic phosphorus. Soil Sci Soc Am J. 1989;53(2):362–6. https://doi.org/10.2136/sssaj1989.03615995005300020008x
  2. 2. Cross AF, Schlesinger WH. A literature review and evaluation of the Hedley fractionation: applications to the biogeochemical cycle of soil phosphorus in natural ecosystems. Geoderma. 1995;64(3–4):197–214. https://doi.org/10.1016/0016-7061(94)00023-4
  3. 3. da Silva LJ, da Silva AP, da Silva AC, Antonangelo JA, Bonetti JA, Fernandes DM, et al. Soil chemical properties effect on phosphorus fractions and maximum adsorption capacity in Brazilian tropical soils. Soil Sci Soc Am J. 2026;90(2):e70223. https://doi.org/10.1002/saj2.70223
  4. 4. Kittrick JA. The free energy of formation of gibbsite and Al(OH)4-from solubility measurements. Soil Sci Soc Am J. 1966;30(5):595–8. https://doi.org/10.2136/sssaj1966.03615995003000050018x
  5. 5. Kumar S, Srivastava A, Gupta A. Effect of organic amendments on availability of different chemical fractions of phosphorus. Agric Sci Dig. 2015;35(2):83–8. https://doi.org/10.5958/0976-0547.2015.00033.6
  6. 6. Rawajfih Z, Khresat S, Ryan J. Soil phosphorus fractions in calcareous Vertisols and Aridisols of northern Jordan. Jordan J Agric Sci. 2010;6(3):411.
  7. 7. Jackson ML. Free oxides, hydroxides and amorphous aluminosilicates. In: Methods of soil analysis: part 1 physical and mineralogical properties, including statistics of measurement and sampling. 1965;9:578–603. https://doi.org/10.2134/agronmonogr9.1.c45
  8. 8. Oades JM. Soil organic matter and structural stability: mechanisms and implications for management. Plant Soil. 1984;76(1):319–37. https://doi.org/10.1007/BF02205590
  9. 9. Syers JK, Williams JD, Walker TW. The determination of total phosphorus in soils and parent materials. N Z J Agric Res. 1968;11(4):757–62. https://doi.org/10.1080/00288233.1968.10422453
  10. 10. Yi C, Zhu J, Chen L, Huang X, Wu R, Zhang H, et al. Speciation of iron and aluminum in relation to phosphorus sorption and supply characteristics of soil aggregates in subtropical forests. Forests. 2023;14(9):1804. https://doi.org/10.3390/f14091804
  11. 11. Lemos JD, Freire FJ, Souza VS, Oliveira EC, Lucena PG, Silva SR, et al. Phosphorus fractions in soils with distinct mineralogy and their relationship with phosphate buffer capacity indicators in Brazil. Acta Sci Agron. 2022;44:e55148. https://doi.org/10.4025/actasciagron.v44i1.55148
  12. 12. Murphy JA, Riley JP. A modified single solution method for the determination of phosphate in natural waters. Anal Chim Acta. 1962;27:31–6. https://doi.org/10.1016/S0003-2670(00)88444-5
  13. 13. Saha BN, Saha S, Poddar P, Chand T. Phosphate fractions as influenced by long-term phosphorus fertilization. Indian J Agric Res. 2013;47(1).
  14. 14. Wang Q, Zhang N, Chen Y, Qin Z, Jin Y, Zhu P, et al. The phosphorus availability in Mollisol is determined by inorganic phosphorus fraction under long-term different phosphorus fertilization regimes. Agronomy. 2022;12(10):2364. https://doi.org/10.3390/agronomy12102364
  15. 15. Olsen SR. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. US Department of Agriculture; 1954.
  16. 16. Chang SC, Jackson ML. Fractionation of soil phosphorus. Soil Sci. 1957;84(2):133–44. https://doi.org/10.1097/00010694-195708000-00005
  17. 17. Milić S, Ninkov J, Vasin J, Zeremski T, Jakšić S, Živanov M, et al. Organic phosphorus fractions in relation to soil aggregate fractions of black soil. Agronomy. 2024;14(5):1022. https://doi.org/10.3390/agronomy14051022
  18. 18. Nunes RD, de Sousa DM, Goedert WJ, de Oliveira LE, Pavinato PS, Pinheiro TD. Distribution of soil phosphorus fractions as a function of long-term soil tillage and phosphate fertilization management. Front Earth Sci. 2020;8:350. https://doi.org/10.3389/feart.2020.00350
  19. 19. Watanabe FS, Olsen SR. Test of an ascorbic acid method for determining phosphorus in water and NaHCO3 extracts from soil. Soil Sci Soc Am J. 1965;29(6):677–8. https://doi.org/10.2136/sssaj1965.03615995002900060025x
  20. 20. Richardson AE, Simpson RJ. Soil microorganisms mediating phosphorus availability update on microbial phosphorus. Plant Physiol. 2011;156(3):989–96. https://doi.org/10.1104/pp.111.175448
  21. 21. Hedley MJ, Stewart JW, Chauhan BS. Changes in inorganic and organic soil phosphorus fractions induced by cultivation practices and by laboratory incubations. Soil Sci Soc Am J. 1982;46(5):970–6. https://doi.org/10.2136/sssaj1982.03615995004600050017x
  22. 22. Prasad R, Shivay YS. Phosphorus× other plant nutrient interactions, reaction products, anion exchange and phosphate fixation in soil and strategies to increase availability of the native and applied P to crop plants: a mini review and critique. Agric Rev. 2021;42(2). https://doi.org/10.18805/ag.R-2028
  23. 23. Warncke DD, Barber SA. Diffusion of zinc in soil: II. The influence of soil bulk density and its interaction with soil moisture. Soil Sci Soc Am J. 1972;36(1):42–6. https://doi.org/10.2136/sssaj1972.03615995003600010009x
  24. 24. Tiecher T, Gatiboni L, Lima AP, Filippi D. Accumulation of phosphorus fractions in contrasting soils under long-term phosphate fertilization. J Environ Qual. 2026. https://doi.org/10.1002/jeq2.70120

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