Plant growth-promoting endophytic bacteria associated with Halocnemum strobilaceum (Pall.) M.Bieb and their plant beneficial traits
DOI:
https://doi.org/10.14719/pst.1605Keywords:
Halocnemum strobilaceum, Bacterial endophytes, Cotton, Plant growth promotionAbstract
Halocnemum strobilaceum (Pall.) M.Bieb. is a halophyte desert plant. The plant is known for its antioxidant, antimicrobial, insecticidal and phytoremediation properties. Halocnemum strobilaceum grows in severe salinity and drought conditions and its’ survival can be associated with activity of endophytic bacteria. The aim of the research was to reveal and study plant growth-promoting endophytic bacteria isolated from Halocnemum strobilaceum (Pall.) M.Bieb. The plants were collected from Kyzylkum desert in Uzbekistan. The endophytic bacteria were isolated from Halocnemum strobilaceum (Pall.) M.Bieb. tissues and screened for cotton (Gossypium hirsutum L.) growth-promoting activity. As a result the most active isolates HAST-2, HAST-7, HAST-9, HAST-10 and HAST-17 were selected. The cotton seeds’ inoculation with these bacterial isolates resulted in significant improvement of seeds germination, root and shoot length, and fresh plant weight due to their ability to fix nitrogen, produce indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, siderophores and solubilize phosphates. The chosen isolates were identified using 16S rRNA gene analysis and registered in GenBank (NCBI) as Bacillus megaterium HAST-2, Bacillus aryabhattai HAST-7, Pseudomonas plecoglossicida HAST-9, Pseudomonas putida HAST-10 and Pseudomonas chlororaphis HAST-17. These strains can be used as bio-inoculants to improve the growth of cotton and other crops.
Downloads
References
Qui XX, Huang ZY, Baskin JM, Baskin CC. Effect of temperature, light and salinity on seed germination and radicle growth of the geographically widespread halophyte shrub Halocnemum strobilaceum. Ann Bot. 2008;101:293-299. https://doi.org/10.1093/aob/mcm047
Mariem S, Mariam K, Mariem BJ, Riadh K. Antioxidant and antimicrobial activities of Halocnemum strobilaceum fractions and their related bioactive molecules identification by GC/MS and HPLC. Res J Recent Sci. 2018;7:1-5
Acheuk F, Lakhdari W, Dahliz A, Abdellaoui K, Moukadem M, Allili S. Toxicity, acethylcolinesterase and glutathione s-transferase effects of Halocnemum strobilaceum crude extract against Tribolium castaneum. A&F. 2018;64:23-33. https://doi.org/10.17707/AgricultForest.64.1.03
Handoussaa H, AbdAllahb W, AbdelMohsenb M. UPLC–ESI-PDA–Msn profiling of phenolics involved in biological activities of the medicinal plant Halocnemum strobilaceum (Pall.). Iran J Pharm Res. 2019;18:422-429
Bobtana F, Elabbar F, Bader N. Evaluation of Halocnemum strobilaceum and Hammada scoparia plants performance for contaminated soil phytoremediation. J Med Chem Sci. 2019;3:126-129. https://doi.org/10.26655/JMCHEMSCI.2019.8.1
Fernando TC, Cruz JA. Profiling and biochemical ?dentification of potential plant growth-promoting endophytic bacteria from Nypa fruticans. Philipp J Crop Sci. 2019;44:77-85. https://doi.org/10.13140/RG.2.2.15641.98408
Egamberdieva D, Shurigin V, Alaylar B, Wirth S, Bellingrath-Kimura SD. Bacterial endophytes from horseradish (Armoracia rusticana G. Gaertn., B.?Mey. ?&? Scherb.) with antimicrobial efficacy against pathogens. Plant Soil Environ. 2020;66:309-316. https://doi.org/10.17221/137/2020-PSE
Shurigin V, Alaylar B, Davranov K, Wirth S, Bellingrath-Kimura SD, Egamberdieva D. Diversity and biological activity of culturable endophytic bacteria associated with marigold (Calendula officinalis L.). AIMS Microbiol. 2021;7(3):336-353. https://doi.org/10.3934/microbiol.2021021
Egamberdieva D, Shurigin V, Gopalakrishnan S, Ram S. Microbial strategies for the improvement of legume production in hostile environments. In: Azooz MM, Ahmad P, editors. Legumes under environmental stress: yield, improvement and adaptations. UK: John Wiley & Sons Ltd; 2015. p. 133-144. https://doi.org/10.1002/9781118917091.ch9
Chung BS, Aslam Z, Kim SW, Kim GG, Kang HS, Ahn JW, et al. A bacterial endophyte, Pseudomonas brassicacearum YC5480, isolated from the root of Artemisia sp. producing antifungal and phytotoxic compounds. Plant Pathol J. 2008;24:461-468. https://doi.org/10.5423/PPJ.2008.24.4.461
Hu HQ, Li XS, He H. Characterization of an antimicrobial material from a newly isolated Bacillus amyloliquefaciens from mangrove for biocontrol of capsicum bacterial wilt. Biol Control. 2010;54:359-365. https://doi.org/10.1016/j.biocontrol.2010.06.015
Shurigin V, Egamberdieva D, Li L, Davranov K, Panosyan H, Birkeland N-K, et al. Endophytic bacteria associated with halophyte Seidlitzia rosmarinus Ehrenb. ex Boiss. from arid land of Uzbekistan and their plant beneficial traits. J Arid Land. 2020;12:730-740. https://doi.org/10.1007/s40333-020-0019-4
Bibi F, Strobel GA, Naseer MI, Yasir M, Al-Ghamdi AAK, Azhar EI. Halophytes-associated endophytic and rhizospheric bacteria: diversity, antagonism and metabolite production. Biocontrol Sci Technol. 2018;28:192-213. https://doi.org/10.1080/09583157.2018.1434868
Coombs JT, Franco CM. Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol. 2003;69:5603-5608. https://doi.org/10.1128/AEM.69.9.5603-5608.2003
Fouda A, Eid AM, Elsaied A, El-Belely EF, Barghoth MG, Azab E, et al. Plant growth promoting endophytic bacterial community inhabiting the leaves of Pulicaria incisa (Lam.) DC inherent to arid regions. Plants. 2021;10:76. https://doi.org/10.3390/plants10010076
Sarwar M, Kremer RJ. Determination of bacterially derived auxins using a microplate method. Lett Appl Microbiol. 1995; 20:282-285. https://doi.org/10.1111/j.1472-765X.1995.tb00446.x
Kuklinsky-Sobral J, Araújo WL, Mendes R, Geraldi IO, Pizzirani-Kleiner AA, Azevedo JL. Isolation and characterization of soybean- associated bacteria and their potential for plant growth promotion. Environ Microbiol. 2004;6:1244-1251. https://doi.org/10.1111/j.1462-2920.2004.00658.x.
Mehta S, Nautiyal CS. An efficient method for qualitative screening of phosphate-solubilizing bacteria. Curr Microbiol. 2001;43:51-56. https://doi.org/10.1007/s002840010259
Bashan Y, Holguin G, Lifshitz R. Isolation and characterization of plant growth-promoting rhizobacteria. In: Glick BR, Thompson JE, editors. Methods in plant molecular biology and biotechnology. USA, FL, Boca Raton: CRC Press; 1993. p. 331-345
Schwyn B, Neilands JB. Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 1987;160:45-46. https://doi.org/10.1016/0003-2697(87)90612-9.
Egamberdieva D, Kucharova Z, Davranov K, Berg G, Makarova N, Azarova T, et al. Bacteria able to control foot and root rot and to promote growth of cucumber in salinated soils. Biol Fertil Soils. 2011;47:197-205. https://doi.org/10.1007/s00374-010-0523-3
Stanier RY, Palleroni NJ, Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966;43:159-271. https://doi.org/10.1099/00221287-43-2-159
Palleroni NJ, Doudoroff M. Some properties and taxonomic subdivisions of the genus Pseudomonas. Annu Rev Phytopathol. 1972; 10:73-100. https://doi.org/10.1146/annurev.py.10.090172.000445
MacFaddin JF, editor. Biochemical tests for identification of medical bacteria. 2nd ed. Baltimore: Williams & Wilkins; 1980
Cowan ST, editor. Cowan and Steel’s manual for the identification of medical bacteria. Cambridge: Cambridge University Press; 1974
Veron M. Nutrition et taxonomie des Enterobacteriaceae et bacteries voisines. I. Methods d'etude des auxanogrammes. Ann Microbiol (Paris). 1975;126:267-274.
Ishimaru K, Akagawa-Matsushita M, Muroga K. Vibrio penaeicida sp. nov., a pathogen of kuruma prawns (Penaeus japonicus). Int J Syst Evol Microbiol. 1995;45:134-138. https://doi.org/10.1099/00207713-45-1-134
Dashti AA, Jadaon MM, Abdulsamad AM, Dashti HM. Heat treatment of bacteria: a simple method of DNA extraction for molecular techniques. KMJ. 2009;41:117-122
Lane DJ. 16S/23S rRNA Sequencing. In: Stackebrandt E, Goodfellow M, editors. Nucleic acid techniques in bacterial systematic. New York: John Wiley and Sons; 1991. p. 115-175
Kruasuwan W, Thamchaipenet A. Diversity of culturable plant growth-promoting bacterial endophytes associated with sugarcane roots and their effect of growth by co-inoculation of diazotrophs and actinomycetes. J Plant Growth Regul. 2016;35:1074-1087. https://doi.org/10.1007/s00344-016-9604-3
Egamberdieva D, Shurigin V, Alaylar B, Ma H, Müller MEH, Wirth S, et al. The effect of biochars and endophytic bacteria on growth and root rot disease incidence of Fusarium infested narrow-leafed lupin (Lupinus angustifolius L.). Microorganisms. 2020;8:496. https://doi.org/10.3390/microorganisms8040496
Leghari SJ, Wahocho NA, Laghari GM, Laghari AH, Bhabhan GM, Talpur KA, et al. Role of nitrogen for plant growth and development: A review. Adv Environ Biol. 2016; 10:209-218
Galloway JN, Cowling EB. Reactive nitrogen and the world: 200 years of change. AMBIO: A Journal of the Human Environment. 2002;31:64-71. https://doi.org/10.1579/0044-7447-31.2.64
Muangthong A, Youpensuk S, Rerkasem B. Isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane. Trop Life Sci Res. 2015;26:41-51
Gao FK, Dai CC, Liu XZ. Mechanisms of fungal endophytes in plant protection against pathogens. Afr J Microbiol Res. 2010;4:1346-1351
Gao D, Tao Y. Current molecular biologic techniques for characterizing environmental microbial community. Front Environ Sci Eng. 2012;6:82-97. https://doi.org/10.1007/s11783-011-0306-6
Yadav AN. Biodiversity and biotechnological applications of host-specific endophytic fungi for sustainable agriculture and allied sectors. ASMI. 2018;1:01-05. https://doi.org/10.31080/ASMI.2018.01.0044
Glick BR, Penrose DM, Li J. A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. J Theor Biol. 1998;190:63-68. https://doi.org/10.1006/jtbi.1997.0532
Downloads
Published
Versions
- 02-04-2022 (2)
- 22-02-2022 (1)
How to Cite
Issue
Section
License
Copyright (c) 2021 Begali Alikulov, Vyacheslav Shurigin, Kakhramon Davranov, Zafar Ismailov
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
