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Research Articles

Vol. 13 No. sp5 (2026): Recent Advances in Agriculture

Nutrified growing media consortia with biocontrol agents enhance growth, physiological traits and flowering of tuberose [Agave amica (Medik.)] cv. Arka Prajwal

DOI
https://doi.org/10.14719/pst.16189
Submitted
18 June 2026
Published
15-09-2026

Abstract

Tuberose [Agave amica (Medik.)] cv. Arka Prajwal is a commercially important ornamental bulbous crop, yet suboptimal growing media constrain its productivity under pot culture. This study evaluated the influence of nutrified enriched growing media consortia comprising organic amendments (farmyard manure, vermicompost, cocopeat) combined with biocontrol agents-Trichoderma viride, Pochonia chlamydosporia and Purpureocillium lilacinum at 50 g pot-¹ on the growth, flowering and physiological traits (total chlorophyll and phenol content) of tuberose under pot culture conditions. A completely randomised design (CRD) with 17 treatments and 3 replications were employed, with fluopyram 34.48 % suspension concentrate (SC) at 0.1 % as the chemical check. Among all the treatments, T11 (red soil + farmyard manure  + vermicompost 1 : 1 : 1 + P. chlamydosporia at 50 g pot-¹) recorded maximum plant height (61.17 cm), leaf area index (2.71), chlorophyll content (1.61 mg g-¹) and phenol content (2.72 mg g-1), spike length (91.70 cm), weight of florets per pot (95.22 g) and days to last floret withering (16.00 days). Principal component analysis identified spike length, weight of florets per pot and days to last floret withering as the primary determinants of treatment-level variation in tuberose productivity. Correlation analysis revealed strong associations between vegetative vigour and floral quality traits. The bio-organic consortia consistently outperformed both the untreated control and chemical standard, demonstrating their potential as sustainable, eco-friendly alternatives to conventional red soil-based cultivation for commercial pot-culture production of tuberose.

References

  1. 1. Pocha PN, Mallikarjun M, Devi GN, Kumar MR. Assessment of improved variety of tuberose (Polianthes tuberosa) Prajwal for yield and economics in western parts of Chittoor District of Andhra Pradesh. Trends Biosci. 2019;12(9):2205–7. https://doi.org/10.5958/2349-4433.2019.00062.X
  2. 2. Ganesh S, Soorianathasundaram K, Kannan M. Studies on effect of plant growth regulators and micronutrients on growth, floral characters and yield of tuberose (Polianthes tuberosa L.) cv. Prajwal. Asian J Hortic. 2013;8(2):696–700.
  3. 3. Rehman SU, De Castro F, Aprile A, Benedetti M, Fanizzi FP. Vermicompost: Enhancing plant growth and combating abiotic and biotic stress. Agronomy. 2023;13(4):1134. https://doi.org/10.3390/agronomy13041134
  4. 4. Pathma J, Sakthivel N. Microbial diversity of vermicompost bacteria that exhibit useful agricultural traits and waste management potential. SpringerPlus. 2012;1:26. https://doi.org/10.1186/2193-1801-1-26
  5. 5. Nair SA, Bharathi TU. Standardisation of substrate composition for pot plant production of tuberose var. Arka Sugandhi. Int J Curr Microbiol App Sci. 2019;8(1):2197–203. https://doi.org/10.20546/ijcmas.2019.801.229
  6. 6. Shaliha BA, Swarnakumari N, Anita B, Thiribhuvanamala G, Suganthi A, Saranya N. The mechanistic pathways of Pochonia chlamydosporia: A biology perspective on nematode suppression and plant promotion. Plant Sci Today. 2025;12:10330. https://doi.org/10.14719/pst.1033
  7. 7. Elissen H, van der Weide R, Gollenbeek L. Effects of vermicompost on plant and soil characteristics: A literature overview. Wageningen: Wageningen Plant Research; 2023. https://doi.org/10.18174/587210
  8. 8. Sarkar D, Hatibarua P, Borbaruah R, Talukdar MC, Naikwad D, Nath DJ. Effect of nursery growing media for quality seedling production of marigold, calendula, dianthus and salvia. Plant Arch. 2025;25(2):1092–102. https://doi.org/10.51470/PLANTARCHIVES.2025.v25.supplement-2.136
  9. 9. Tyśkiewicz R, Nowak A, Ozimek E, Jaroszuk-Ściseł J. Trichoderma: The current status of its application in agriculture for the biocontrol of fungal phytopathogens and stimulation of plant growth. Int J Mol Sci. 2022;23(4):2329. https://doi.org/10.3390/ijms23042329
  10. 10. Dallemole-Giaretta R, Freitas LG, Lopes EA, Silva MD, Kasuya MC, Ferraz S. Pochonia chlamydosporia promove crescimento de tomateiro e alface. Acta Sci Agron. 2015;37(4):417–23. https://doi.org/10.4025/actasciagron.v37i4.25042
  11. 11. Khan M, Tanaka K. Purpureocillium lilacinum for plant growth promotion and biocontrol against root-knot nematodes infecting eggplant. PLoS One. 2023;18(3):e0283550. https://doi.org/10.1371/journal.pone.0283550
  12. 12. Contreras-Cornejo HA, Schmoll M, Esquivel-Ayala BA, González-Esquivel CE, Rocha-Ramírez V, Larsen J. Mechanisms for plant growth promotion activated by Trichoderma in natural and managed terrestrial ecosystems. Microbiol Res. 2024;281:127621. https://doi.org/10.1016/j.micres.2024.127621
  13. 13. Parveen A, Tanaka K, Khan M. Biocontrol efficacy of Pochonia chlamydosporia against root-knot nematode Meloidogyne javanica in eggplant and its impact on plant growth. Sci Rep. 2025;15(1):36990. https://doi.org/10.1038/s41598-025-99527-2
  14. 14. Saravani M, Boogar AR, Aran M, Ramezan D, Zargar M, Diakite S. Optimizing tuberose (Polianthes tuberosa L.) production using mycorrhiza and biostimulants to enhance water-deficit tolerance. Horticulturae. 2025;11(1):34. https://doi.org/10.3390/horticulturae11010034
  15. 15. Sun T, Li M, Saleem M, Zhang X, Zhang Q. The fungicide fluopyram promotes pepper growth by increasing the abundance of P-solubilizing and N-fixing bacteria. Ecotoxicol Environ Saf. 2020;188:109947. https://doi.org/10.1016/j.ecoenv.2019.109947
  16. 16. Subbiah BV, Asija GL. A rapid procedure for estimation of available nitrogen in soils. Curr Sci. 1956;25:259–60.
  17. 17. Jackson ML. Soil chemical analysis. New Delhi: Prentice Hall of India Pvt. Ltd.; 1973.
  18. 18. Walkley A, Black IA. An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci. 1934;37(1):29–38.
  19. 19. Arnon DI. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 1949;24(1):1–15. https://doi.org/10.1104/pp.24.1.1
  20. 20. Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. In: Packer L, editor. Methods in enzymology. Vol. 299. San Diego: Academic Press; 1999. p. 152–78. https://doi.org/10.1016/S0076-6879(99)99017-1
  21. 21. Atiyeh RM, Arancon N, Edwards CA, Metzger JD. Influence of earthworm-processed pig manure on the growth and yield of greenhouse tomatoes. Bioresour Technol. 2000;75(3):175–80.
  22. 22. Edwards CA, Arancon NQ. The use of earthworms in the breakdown of organic wastes to produce vermicomposts and animal feed protein. In: Edwards CA, editor. Earthworm ecology. 2nd ed. Boca Raton: CRC Press; 2004. p. 345–79.
  23. 23. Kerry BR. Rhizosphere interactions and the exploitation of microbial agents for the biological control of plant-parasitic nematodes. Annu Rev Phytopathol. 2000;38:423–41. https://doi.org/10.1146/annurev.phyto.38.1.423
  24. 24. Lopez-Llorca LV, Gómez-Vidal S, Monfort E, Larriba E, Casado-Vela J, Elortza F, et al. Expression of serine proteases in egg-parasitic nematophagous fungi during barley root colonization. Fungal Genet Biol. 2010;47(4):342–5. https://doi.org/10.1016/j.fgb.2010.01.004
  25. 25. Karagöz FP, Dursun A, Tekiner N, Kul R, Kotan R. Efficacy of vermicompost and/or plant growth-promoting bacteria on the plant growth and development in gladiolus. Ornam Hortic. 2019;25(2):180–8. https://doi.org/10.14295/oh.v25i2.2023
  26. 26. Yadav R, Beniwal BS, Dalal RS, Kumar S. Influence of vermicompost and bio-fertilizers on growth and flowering of tuberose (Polianthes tuberosa L.) cv. Prajwal. Int J Plant Soil Sci. 2023;35(16):113–20. https://doi.org/10.9734/ijpss/2023/v35i163136
  27. 27. Chaya MK, Rao MS. Bio-management of Meloidogyne incognita on okra using a formulation of Pochonia chlamydosporia. Pest Manag Hortic Ecosyst. 2012;18(2):170–3.
  28. 28. Rajamanickam C, Ravindran C. Bio-management of root-knot nematode (Meloidogyne incognita) in tuberose (Polianthes tuberosa L.). J Pharmacogn Phytochem. 2021;10(1):1693–5. https://doi.org/10.22271/phyto.2021.v10.i1x.13587
  29. 29. Kumari M, Chakma J, Singh SP. Evaluation of synergistic effects of vermicompost and beneficial microbes on pea. Curr J Appl Sci Technol. 2020;39(1):137–47. https://doi.org/10.9734/CJAST/2020/v39i130489
  30. 30. Khatri R, Adhikari S, Pokharel NP, Adhikari S, Khanal Y, Thapaliya KP, et al. Evaluating the effect of biofertilizer dosages on growth and yield parameters of cauliflower in Mugu District, Nepal. Int J Agric Environ Food Sci. 2025;9(3):733–42. https://doi.org/10.31015/2025.3.11
  31. 31. Nawrin KS, Uddin MJ, Ali AH, Rahman MK. Effects of boron and vermicompost on growth, yield and nutrient content of chilli (Capsicum annuum L.). J Biodivers Conserv Bioresour Manag. 2021;6(1):31–6. https://doi.org/10.3329/jbcbm.v6i1.51329
  32. 32. Rigobelo EC, Nicodemo D, Babalola OO, Desoignies N. Purpureocillium lilacinum as an agent of nematode control and plant growth-promoting fungi. Agronomy. 2024;14(6):1225. https://doi.org/10.3390/agronomy14061225
  33. 33. Theunissen J, Ndakidemi PA, Laubscher CP. Potential of vermicompost produced from plant waste on the growth and nutrient status in vegetable production. Int J Phys Sci. 2010;5(13):1964–73.
  34. 34. Ghahremani Z, Escudero N, Saus E, Gabaldón T, Sorribas FJ. Pochonia chlamydosporia induces plant-dependent systemic resistance to Meloidogyne incognita. Front Plant Sci. 2019;10:945. https://doi.org/10.3389/fpls.2019.00945
  35. 35. Habib ZF, Hossain MI, Mazed HK, Sultana T, Yeasmin M. Assessment of different soil amendment effects on tuberose bulb quality and quantity. IOSR J Agric Vet Sci. 2016;9(8):16–9. https://doi.org/10.9790/2380-0908021619
  36. 36. Padaganur VG, Mokashi AN, Patil VS. Flowering, flower quality and yield of tuberose (Polianthes tuberosa L.) as influenced by vermicompost, farmyard manure and fertilizers. Karnataka J Agric Sci. 2005;18(3):729–34.
  37. 37. Sajjan G. Impact of bio-fertilisers and vermicompost on growth, flowering and vase life of gerbera (Gerbera jamesonii) under polyhouse conditions. Int J Agric Extension Soc Dev. 2023;6(1):117–22. https://doi.org/10.33545/26180723.2023.v6.i1b.2987
  38. 38. Nagesh M, Singh KP. Bio-management of Meloidogyne incognita on Polianthes tuberosa using Glomus mosseae and Pochonia chlamydosporia as bulb dressing in combination with neem cake. J Ornam Hortic. 2004;7(1):45–51.
  39. 39. Zavala-González EA, Escudero N, López-Moya F, Aranda-Martinez A, Exposito A, Ricaño-Rodríguez J, et al. Some isolates of the nematophagous fungus Pochonia chlamydosporia promote root growth and reduce flowering time of tomato. Ann Appl Biol. 2015;166(3):472–83. https://doi.org/10.1111/aab.12199
  40. 40. Srivastava RK, Pareek N, Chand S, Bhuj BD, Pant K, Belwal S. Rhizospheric fungal and bacterial bio-agents in flowering and bulb of tuberose (Polianthes tuberosa L.). Bangladesh J Bot. 2022;51(3):565–72. https://doi.org/10.3329/bjb.v51i3.62003
  41. 41. Shirsat PR, Kuchanwar OD, Ingle SN, Zalte S, Abgad NP. Effect of integrated nutrient management on yield and quality of tuberose grown on Vertisol. Asian J Soil Sci. 2015;10(2):210–14. https://doi.org/10.15740/HAS/AJSS/10.2/210-214
  42. 42. Chawla SL, Patel MA, Patil S, Bhatt D, Patel RB. Effect of land configuration and integrated nutrient management on growth, quality and yield of tuberose (Polianthes tuberosa) var. Prajwal. Indian J Agric Sci. 2018;88(12):1854–8. https://doi.org/10.56093/ijas.v88i12.85435
  43. 43. Manisha, Beniwal BS, Sheoran S, Yadav R. Response of organic manures and their levels on growth and spike yield of tuberose (Polianthes tuberosa L.) cv. Prajwal. Plant Arch. 2025;25(2):1658–62. https://doi.org/10.51470/PLANTARCHIVES.2025.v25.no.2.240
  44. 44. Ikram S, Habib U, Khalid N. Effect of different potting media combinations on growth and vase life of tuberose (Polianthes tuberosa Linn.). Pak J Agric Sci. 2012;49(2):121–5.
  45. 45. Mazhabi M, Nemati H, Rouhani H, Tehranifar A, Moghadam EM, Kaveh H, et al. The effect of Trichoderma on Polianthes qualitative and quantitative properties. J Anim Plant Sci. 2011;21(3):617–21.
  46. 46. Nosir WS. Trichoderma harzianum as a growth promoter and bio-control agent against Fusarium oxysporum f. sp. tuberosi. Adv Crop Sci Technol. 2016;4:217. https://doi.org/10.4172/2329-8863.1000217
  47. 47. Wasim M, Gupta NK, Dubey S, Mohanty A. Effect of inorganic fertilisers in combination with bio-fertilisers on growth and yield of tuberose (Polianthes tuberosa). Indian Hort J. 2014;4(1):37–42.
  48. 48. Gogoi K, Talukdar MC, Talukdar P. Correlation coefficient and path analysis in tuberose (Polianthes tuberosa L.) genotypes. Res Crops. 2019;20(1):150–60. https://doi.org/10.31830/2348-7542.2019.021
  49. 49. Kayalvizhi K, Kannan M, Ganga M. Mean performance, correlation coefficient and path coefficient analysis for yield and yield attributing characters in F₁ population of tuberose (Polianthes tuberosa L.). Madras Agric J. 2023;103. https://doi.org/10.29321/MAJ.10.001034
  50. 50. Gandhi PD, Bharathi U. Correlation and path coefficient analysis in tuberose cultivars single. Agric Sci Dig. 2020;40(2):134–8. https://doi.org/10.18805/ag.D-5028
  51. 51. Ranchana P, Kannan M, Jawaharlal M. Correlation and path analysis studies in double type tuberose. Asian J Hortic. 2015;10(1):113–7. https://doi.org/10.15740/HAS/TAJH/10.1/113-117
  52. 52. Naznin A, Hossain MM, Ara KA, Hoque A, Islam M, Hasan T. Influence of organic amendments and bio-control agent on yield and quality of tuberose. J Hortic. 2015;2(4):1–8. https://doi.org/10.4172/2376-0354.1000156
  53. 53. Kavitha K, Thirukumaran K. Integrated management of nematode disease complex in tuberose (Polianthes tuberosa L.). J Krishi Vigyan. 2019;8(1):170–3. https://doi.org/10.5958/2349-4433.2019.00091.6
  54. 54. Rajaselvam M, Sudhagar R, Kumaresan M. Determining the effect of integrated nutrient management (INM) on growth and flower yield of tuberose (Polianthes tuberosa L.) cv. Prajwal. Int J Plant Soil Sci. 2024;36(6). https://doi.org/10.9734/IJPSS/2024/v36i64600

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