Hepatoprotective potency of Lagenandra toxicaria and Ariopsis peltata against CCl4 induced liver fibrosis in Wistar rats

Authors

  • B Akarsha Department of Applied Botany, Mangalore University, Mangalore, Konaje – 574 199, India
  • G Krishnakumar Department of Applied Botany, Mangalore University, Mangalore, Konaje – 574 199, India
  • Prasanna Shama Khandige Department of Pharmacology, NGSM Institute of Pharmaceutical Sciences Nitte (Deemed to be University) Mangalore. Deralakatte – 575 018, India

DOI:

https://doi.org/10.14719/pst.1691

Keywords:

Lagenandra toxicaria, Ariopsis peltata, GC-MS, Liver fibrosis, anti-inflammatory

Abstract

Lagenandra toxicaria (LT) and Ariopsis peltata (AP) belongs to the family Araceae. LT is being traditionally used to cure bilious symptoms and wound healing. AP leaves are edible and the rhizome has medicinal value. With such a wide range of medicinal applications, it's essential to scientifically authenticate traditional usage and find the plant's bioactive components. Here, we have examined the anti-inflammatory and hepatoprotective potency of these plant extracts. The in-vivo liver fibrosis was induced in Wistar male rats using CCl4 and was treated with various concentrations of the methanol and water extracts of the plant rhizomes. In the in-vitro anti-inflammatory assay, LT methanol extract showed 42 % protection at 600 mg/ml which is higher than the other extracts. Among the AP and LT extracts, the 600 mg/kg methanol extract of LT treated rats showed a decreased (p<0.05) serum alanine/aspartate aminotransferase and total bilirubin levels. The plant extract brought about an amelioration of CCl4 induced pathological effects and a significant reduction in the severity of inflammation, fatty metamorphosis, necrosis and liver fibrosis. Overall, the results suggest that LT rhizome could be effective in the treatment of liver fibrosis. It is possible that this is related to the presence of anti-inflammatory and antioxidant chemicals such as 9-octadecenamide, flavonoids and phenols in the extract.

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References

John LA, Kannappan N, Manojkumar P. Evaluation of Hepatoprotective activity in methanolic extract of Aerial parts of Hibiscus surattensis. Res J Pharm Technol. 2020;13(10):4635-40. http://dx.doi.org/10.5958/0974-360X.2020.00816.1.

Asrani SK, Devarbhavi H, Eaton J, Kamath PS. Burden of liver diseases in the world. J Hepatol. 2019;70(1):151-71. https://doi.org/10.1016/j.jhep.2018.09.014.

Guo Y, Liang X, Meng M, Chen H, Wei X, Li M et al. Hepatoprotective effects of Yulangsan flavone against carbon tetrachloride (CCl4)-induced hepatic fibrosis in rats. Phytomedicine. 2017;33:28-35. https://doi.org/10.1016/j.phymed.2017.07.005.

Latief U, Ahmad R. Herbal remedies for liver fibrosis: A review on the mode of action of fifty herbs. J Tradit Complement Med. 2018;8(3):352-60. https://doi.org/10.1016/j.jtcme.2017.07.002.

Lam P, Cheung F, Tan HY, Wang N, Yuen MF, Feng Y. Hepatoprotective effects of Chinese medicinal herbs: a focus on anti-inflammatory and anti-oxidative activities. Int J Mol Sci. 2016;17(4):465. https://doi.org/10.3390/ijms17040465

Zhang A, Sun H, Wang X. Recent advances in natural products from plants for treatment of liver diseases. Eur J Med Chem. 2013;63:570-77. https://doi.org/10.1016/j.ejmech.2012.12.062.

El-Tantawy WH, Temraz A. Anti-fibrotic activity of natural products, herbal extracts and nutritional components for prevention of liver fibrosis: review. Arch Physiol Biochem. 2019;0(0):1-12. https://doi.org/10.1080/13813455.2019.1684952

Tuse TA, Harle UN, Bore VV. Hepatoprotective activity of Colocasia antiquorum against experimentally induced liver injury in rats. Malyasian J Pharma Sci. 2009;7(2):99-112.

Sharstry RA, Biradar SM, Mahadevan KM, Habbu PV. Isolation and characterization of secondary metabolite from Amorphophallus paeoniifolius for hepatoprotective activity. Res J Pharm Biol Chem Sci. 2010;1(4):429-37.

Rahman MA, Solaiman M, Haque ME, Das AK. Analgesic and anti-inflammatory activities of Alocasia indica (Roxb.) Schott. Orient Pharm Exp Med. 2011;11(3):143-46. https://doi.org/10.1007/s13596-011-0027-1

Annie Sulochana Selvakumari P. Pharmacognostical standardisation of Lagenandra toxicaria Dalz. Malays J Sci. 2014;33(2):163-75. https://doi.org/10.22452/mjs.vol33no2.5

Bhat S, Mulgund GS, Hegde S, Hegde HV. Pharmacognostic evaluation and phytochemical analysis of leaf and tuber of Ariopsis peltata Nimmo. J Pharmacogn Phytochem. 2019;8(1):1608-15.

Devi HP, Mazumder PB. Methanolic extract of Curcuma caesia Roxb. prevents the toxicity caused by cyclophosphamide to bone marrow cells, liver and kidney of mice. Pharmacogn Res. 2016;8(1):43. https://doi.org/10.4103%2F0974-8490.171106

Khanal S, Shakya N, Thapa K, Pant DR. Phytochemical investigation of crude methanol extracts of different species of Swertia from Nepal. BMC Res Notes. 2015;8(1):1-9. https://doi.org/10.1186/s13104-015-1753-0

Bhalodia NR, Nariya PB, Acharya RN, Shukla VJ. In vitro antioxidant activity of hydro alcoholic extract from the fruit pulp of Cassia fistula Linn. Ayu. 2013;34(2):209-14. https://doi.org/10.4103/0974-8520.119684

Saleem TM, Azeem AK, Dilip C, Sankar C, Prasanth NV, Duraisami R. Anti–inflammatory activity of the leaf extacts of Gendarussa vulgaris Nees. Asian Pac J Trop Biomed. 2011;1(2):147-49. https://doi.org/10.1016/S2221-1691(11)60014-2

Vogel HG. Drug discovery and evaluation: pharmacological assays. Springer Science & Business Media; 2002. https://doi.org/10.1007/978-3-540-70995-4

Wang R, Wang J, Song F, Li S, Yuan Y. Tanshinol ameliorates CCI4-induced liver fibrosis in rats through the regulation of Nrf2/HO-1 and NF-?b/I?B? signaling pathway. Drug Des Devel Ther. 2018;12:1281-92. https://doi.org/10.2147/DDDT.S159546.

Jayakumar T, Ramesh E, Geraldine P. Antioxidant activity of the oyster mushroom, Pleurotus ostreatus on CCl4-induced liver injury in rats. Food Chem Toxicol. 2006 Dec 1;44(12):1989-96. https://doi.org/10.1016/j.fct.2006.06.025

Gui SY, Wei W, Wang H, Wu L, Sun WY, Chen W bi, et al. Effects and mechanisms of crude astragalosides fraction on liver fibrosis in rats. J Ethnopharmacol. 2006;103(2):154-59. https://doi.org/10.1016/j.jep.2005.07.025

Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972;47(2):389-94. https://doi.org/10.1016/0003-2697(72)90132-7

Hamza AA. Ameliorative effects of Moringa oleifera Lam. seed extract on liver fibrosis in rats. Food Chem Toxicol. 2010;48(1):345-55. https://doi.org/10.1016/j.fct.2009.10.022

Czaja AJ. Hepatic inflammation and progressive liver fibrosis in chronic liver disease. World J Gastroenterol WJG. 2014;20(10):2515. https://doi.org/10.3748/wjg.v20.i10.2515

Heeba GH, Mahmoud ME. Therapeutic potential of morin against liver fibrosis in rats: modulation of oxidative stress, cytokine production and nuclear factor kappa B. Environ Toxicol Pharmacol. 2014;37(2):662-71. https://doi.org/10.1016/j.etap.2014.01.026

Jia R, Li Y, Cao L, Du J, Zheng T, Qian H, et al. Antioxidative, anti-inflammatory and hepatoprotective effects of resveratrol on oxidative stress-induced liver damage in tilapia (Oreochromis niloticus). Comp Biochem Physiol Part C Toxicol Pharmacol. 2019;215:56-66. https://doi.org/10.1016/j.cbpc.2018.10.002

Anosike CA, Obidoa O, Ezeanyika LU. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU J Pharm Sci. 2012;20(1):1-7. . https://doi.org/10.1186/2008-2231-20-76

Yanguas SC, Cogliati B, Willebrords J, Maes M, Colle I, Van den Bossche B et al. Experimental models of liver fibrosis. Arch Toxicol. 2016;90(5):1025-48. https://doi.org/10.1007/s00204-015-1543-4

Roh JS, Sohn DH. Damage-associated molecular patterns in inflammatory diseases. Immune Netw. 2018 Aug 1;18(4). https://doi.org/10.4110/in.2018.18.e27

Dong X, Liu J, Xu Y, Cao H. Role of macrophages in experimental liver injury and repair in mice. Exp Ther Med. 2019 Mar 27;17(5):3835. https://doi.org/10.3892/etm.2019.7450

Weng TC, Shen CC, Chiu YT, Lin YL, Kuo CD, Huang YT. Inhibitory effects of armepavine against hepatic fibrosis in rats. J Biomed Sci. 2009;16(1):1-13. https://doi.org/10.1186/1423-0127-16-78

Wang R, Yu XY, Guo ZY, Wang YJ, Wu Y, Yuan YF. Inhibitory effects of salvianolic acid B on CCl4-induced hepatic fibrosis through regulating NF-?B/I?B? signaling. J Ethnopharmacol. 2012;144(3):592-98. https://doi.org/10.1016/j.jep.2012.09.048

Medina J, Moreno-Otero R. Pathophysiological basis for antioxidant therapy in chronic liver disease. Drugs. 2005;65(17):2445-61. https://doi.org/10.2165/00003495-200565170-00003

Vuda M, D’Souza R, Upadhya S, Kumar V, Rao N, Kumar V et al. Hepatoprotective and antioxidant activity of aqueous extract of Hybanthus enneaspermus against CCl4-induced liver injury in rats. Exp Toxicol Pathol. 2012;64(7–8):855-59. https://doi.org/10.1016/j.etp.2011.03.006

Hoffmann C, Djerir NEH, Danckaert A, Fernandes J, Roux P, Charrueau C, et al. Hepatic stellate cell hypertrophy is associated with metabolic liver fibrosis. Sci Rep. 2020;10(1):1-13. https://doi.org/10.1038/s41598-020-60615-0

Chouhan S, Guleria S. Anti-inflammatory activity of medicinal plants: Present status and future perspectives. In: Botanical Leads for Drug Discovery. Springer; 2020. p. 67-92. https://doi.org/10.1007/978-981-15-5917-4_4

Olaoluwa OO, James DA, Adigun OA. Volatile oil analysis of aerial parts of Boerhavia coccinea (Mill.). Nat Prod Res. 2018;32(8):959-62. https://doi.org/10.1080/14786419.2017.1366477

Moon SM, Lee SA, Hong JH, Kim JS, Kim DK, Kim CS. Oleamide suppresses inflammatory responses in LPS-induced RAW264. 7 murine macrophages and alleviates paw edema in a carrageenan-induced inflammatory rat model. Int Immunopharmacol. 2018;56:179-85. https://doi.org/10.1016/j.intimp.2018.01.032

Yang WS, Lee SR, Jeong YJ, Park DW, Cho YM, Joo HM et al. Antiallergic activity of ethanol extracts of Arctium lappa L. undried roots and its active compound, oleamide, in regulating Fc?RI-mediated and MAPK signaling in RBL-2H3 cells. J Agric Food Chem. 2016;64(18):3564-73. https://doi.org/10.1021/acs.jafc.6b00425

Ano Y, Ozawa M, Kutsukake T, Sugiyama S, Uchida K, Yoshida A et al. Preventive effects of a fermented dairy product against Alzheimer’s disease and identification of a novel oleamide with enhanced microglial phagocytosis and anti-inflammatory activity. PLoS One. 2015;10(3):e0118512. https://doi.org/10.1371/journal.pone.0118512

Seki E, Schwabe RF. Hepatic inflammation and fibrosis: functional links and key pathways. Hepatology. 2015;61(3):1066-79. https://doi.org/10.1002/hep.27332

Oh YT, Lee JY, Lee J, Lee JH, Kim JE, Ha J et al. Oleamide suppresses lipopolysaccharide-induced expression of iNOS and COX-2 through inhibition of NF-?B activation in BV2 murine microglial cells. Neurosci Lett. 2010;474(3):148-53. https://doi.org/10.1016/j.neulet.2010.03.026

Published

09-09-2022 — Updated on 01-10-2022

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1.
Akarsha B, Krishnakumar G, Khandige PS. Hepatoprotective potency of Lagenandra toxicaria and Ariopsis peltata against CCl4 induced liver fibrosis in Wistar rats. Plant Sci. Today [Internet]. 2022 Oct. 1 [cited 2024 Nov. 4];9(4):855-66. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1691

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Research Articles