Strategies for Successful Acclimatization and Hardening of In Vitro Regenerated Plants: Challenges and Innovations in Micropropagation Techniques

Nishesh Sharma; Nishant Kumar; Jerin James; Sonika Kalia; Shivani  Joshi

doi:10.14719/pst.2376

Authors

Nishesh Sharma Department of Biotechnology, School of Applied & Lifesciences (SALS), Uttaranchal University, Dehradun, 248007 - India https://orcid.org/0000-0003-4350-5175
Nishant Kumar Department of Biotechnology, School of Applied & Lifesciences (SALS), Uttaranchal University, Dehradun, 248007 - India https://orcid.org/0009-0004-9936-0950
Jerin James Department of Biotechnology, School of Applied & Lifesciences (SALS), Uttaranchal University, Dehradun, 248007 - India https://orcid.org/0000-0003-4215-5812
Sonika Kalia Department of Biotechnology, School of Applied & Lifesciences (SALS), Uttaranchal University, Dehradun, 248007 - India https://orcid.org/0000-0001-9995-1276
Shivani Joshi Department of Biotechnology, School of Applied & Lifesciences (SALS), Uttaranchal University, Dehradun, 248007 - India https://orcid.org/0000-0001-9623-6306

DOI:

https://doi.org/10.14719/pst.2376

Keywords:

Acclimatization, hardening, ex vitro rooting, hydroponics, in-vitro, biotization

Abstract

The micropropagation technique serves as an effective approach for conserving and propagating numerous plant species. Challenges to its success encompass explant selection, media composition, hormone concentration, microbial contamination, incubation conditions, and photoperiod. Beyond these factors, the veracity of tissue culture hinges on successful acclimatization of in vitro regenerated plants to their natural surroundings. Tissue culture-derived plants exhibit characteristic variations like altered nutrition, reduced cuticular wax, non-functional stomata, etc. During transition to natural conditions, a significant portion of micropropagated plants face survival challenges. Studies propose gradual acclimatization processes for smooth adjustment. Ex vitro rooting is advocated for economic, simple, and enhanced survival outcomes. Hydroponics, photoautotrophic acclimatization, and biotization strategies also improve post-transplantation survival. This study evaluates diverse strategies for achieving successful acclimatization of in vitro regenerated plants.

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