GC-MS analysis and cytotoxic activity of the n-hexane fraction from Curcuma sahuynhensis Škornick. & N.S.Lý leaves collected in Vietnam
DOI:
https://doi.org/10.14719/pst.2881Keywords:
Curcuma sahuynhensis, anticancer, cytotoxicity, endemic plant, GC-MSAbstract
Curcuma sahuynhensis Škornick. & N.S.Lý is an endemic plant in Vietnam that has been used by the Sa Huynh people as a spice and medicine to cure illnesses linked to digestive disorders. Very little information is available so far about the chemical composition and biological effects of C. sahuynhensis. To find new pharmaceutical ingredients, the in vitro cytotoxic effect and the chemical profile of C. sahuynhensis leaf extract were investigated. In this study, the percolation method and liquid-liquid dispersion technique were used to extract dry sample powder. The chemical composition was detected by gas chromatography-mass spectrometry (GC-MS). The Sulforhodamine B and MTT methods were used to determine the cytotoxic activity. The chemical composition analysis showed that the leaf extract contained 14 components. The major components in the n-hexane extract were 6,10,14-trimethylpentadecan-2-one, phytol, 1-ethylbutyl hydroperoxide, isoborneol, 1-methylpentyl hydroperoxide, and neophytadiene. On human cancer cell lines, namely MFC-7, SK-LU-1, Hela, MKN-7, and HL-60, the leaf extract showed dose-dependent cytotoxic activity, with IC50 values ranging from 221.70±10.24 to 369.42±10.60 ?g/mL. The present study provides significant information on the chemical components and cytotoxic effects of the n-hexane extract from C. sahuynhensis leaves. The findings will continue to be crucial in future research on the evaluation of secondary metabolite compound analysis for cancer therapeutic effects.
Downloads
References
Mattiuzzi C, Lippi G. Current cancer epidemiology. J Epidemiol Glob Health. 2019;9(4):217-22. https://doi.org/10.2991/jegh.k.191008.001
Wahlang JB, Laishram PD, Brahma DK, Sarkar C, Lahon J, Nongkynrih BS. Adverse drug reactions due to cancer chemotherapy in a tertiary care teaching hospital. Ther Adv Drug Saf. 2017;8(2):61-66. https://doi.org/10.1177/2042098616672572
Manurung H, Susanto D, Kusumawati E, Aryani R, Nugroho RA, Kusuma R et al. Phytochemical, GC-MS analysis and antioxidant activities of leaf methanolic extract of Lai (Durio kutejensis), the endemic plant of Kalimantan, Indonesia. Biodiversitas. 2022;23(11):5566-73. https://doi.org/10.13057/biodiv/d231104
Safarzadeh E, Shotorbani SS, Baradaran B. Herbal medicine as inducers of apoptosis in cancer treatment. Adv Pharm Bull. 2014;4(Suppl 1):421-27. https://doi.org/10.5681/apb.2014.062
Kooti W, Servatyari K, Behzadifar M, Asadi-Samani M, Sadeghi F, Nouri B et al. Effective medicinal plant in cancer treatment, part 2: review study. J Evid Based Complementary Altern Med. 2017;22(4):982-95. https://doi.org/10.1177/2156587217696927
Kaliyadasa E, Samarasinghe BA. A review on golden species of Zingiberaceae family around the world: Genus Curcuma. Afr J Agric Res. 2019;14(9):519-31. https://doi.org/10.5897/AJAR2018.13755
Jambunathan S, Bangarusamy D, Padma PR, Sundaravadivelu S. Cytotoxic activity of the methanolic extract of leaves and rhizomes of Curcuma amada Roxb. against breast cancer cell lines. Asian Pac J Trop Med. 2014;7(Suppl 1):S405-S409. https://doi.org/10.1016/S1995-7645(14)60266-2
Pintatum A, Maneerat W, Logie E, Tuenter E, Sakavitsi ME, Pieters L et al. In vitro anti-inflammatory, anti-oxidant and cytotoxic activities of four Curcuma species and the isolation of compounds from Curcuma aromatica rhizome. Biomolecules. 2020;10(5):799. https://doi.org/10.3390/biom10050799
Lobo R, Prabhu KS, Shirwaikar A, Shirwaikar A. Curcuma zedoaria Rosc. (white turmeric): a review of its chemical, pharmacological and ethnomedicinal properties. J Pharm Pharmacol. 2009;61(1):13-21. https://doi.org/10.1211/jpp.61.01.0003
Zohmachhuana A, Lalnunmawia F, Mathipi V, Lalrinzuali K, Kumar NS. Curcuma aeruginosa Roxb. exhibits cytotoxicity in A-549 and HeLa cells by inducing apoptosis through caspase-dependent pathways. Biomed Pharmacother. 2022;150:113039. https://doi.org/10.1016/j.biopha.2022.113039
Viriyaadhammaa N, Saiai A, Neimkhum W, Nirachonkul W, Chaiyana W, Chiampanichayakul S et al. Cytotoxic and antiproliferative effects of diarylheptanoids isolated from Curcuma comosa rhizomes on leukaemic cells. Molecules. 2020;25(22):5476. https://doi.org/10.3390/molecules25225476
Van Chen TRAN, Lam DNX, Thong CLT, Nguyen DD, Nhi NTT, Triet NT. Morphological characters, pharmacognostical parameters and preliminary phytochemical screening of Curcuma sahuynhensis Škorni?k. & N.S. Lý in Quang Ngai Province, Vietnam. Biodiversitas. 2022;23(8):3907-20. https://doi.org/10.13057/biodiv/d230807
Reda EH, Shakour ZTA, El-Halawany AM, El-Kashoury ESA, Shams KA, Mohamed TA et al. Comparative study on the essential oils from five wild Egyptian Centaurea species: Effective extraction techniques, antimicrobial activity and in-silico analyses. Antibiotics. 2021;10(3):252. https://doi.org/10.3390/antibiotics10030252
Ch R, Chevallier O, McCarron P, McGrath TF, Wu D, Kapil AP et al. Metabolomic fingerprinting of volatile organic compounds for the geographical discrimination of rice samples from China, Vietnam and India. Food Chem. 2021;334:127553. https://doi.org/10.1016/j.foodchem.2020.127553
Tran CV, Vo TM, Bui PT, Duong DNP, Duong LXN, Dinh DQ et al. Phytochemical screening, antioxidant activity and ?-glucosidase inhibitability of Bauhinia x blakeana Dunn. leaf and flower extracts from Vietnam. Trop J Nat Prod Res. 2023;7(4):2737-43. https://doi.org/10.26538/tjnpr/v7i4.11.
Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D et al. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 1990;82(13):1107-12. https://doi.org/10.1093/jnci/82.13.1107
Lakshmipriya T, Soumya T, Jayasree PR, Manish Kumar PR. Selective induction of DNA damage, G2 abrogation and mitochondrial apoptosis by leaf extract of traditional medicinal plant Wrightia arborea in K562 cells. Protoplasma. 2018;255:203-16. https://doi.org/10.1007/s00709-017-1137-5
Sam LN, Huong LT, Minh PN, Vinh BT, Dai DN, Setzer WN et al. Chemical composition and antimicrobial activity of the rhizome essential oil of Curcuma sahuynhensis from Vietnam. J Essent Oil-Bear Plants. 2020;23(4):803-09. https://doi.org/10.1080/0972060X.2020.1821789
Xiao Q, Mu X, Liu J, Li B, Liu H, Zhang B et al. Plant metabolomics: A new strategy and tool for quality evaluation of Chinese medicinal materials. Chin Med. 2022;17(1):45. https://doi.org/10.1186/s13020-022-00601-y
Zhao M, Xiao L, Linghu K-G, Zhao G, Chen Q, Shen L et al. Comprehensive comparison on the anti-inflammation and GC-MS-based metabolomics discrimination between Bupleuri chinense DC. and B. scorzonerifolium Willd. Front Pharmacol. 2022;13:1005011. https://doi.org/10.3389/fphar.2022.1005011
Balogun OS, Ajayi OS, Adeleke AJ. Hexahydrofarnesyl acetone-rich extractives from Hildegardia barteri. J Herbs Spices Med Plants. 2017;23(4):393-400. https://doi.org/10.1080/10496475.2017.1350614
Nazli? M, Kremer D, Grubeši? RJ, Soldo B, Vuko E, Stabentheiner E et al. Endemic Veronica saturejoides Vis. ssp. saturejoides–Chemical composition and antioxidant activity of free volatile compounds. Plants. 2020;9(12):1646. https://doi.org/10.3390/plants9121646
Altir NKM, Ali AMA, Gaafar ARZ, Qahtan AA, Abdel-Salam EM, Alshameri A et al. Phytochemical profile, in vitro antioxidant and anti-protein denaturation activities of Curcuma longa L. rhizome and leaves. Open Chem. 2021;19(1):945-52. https://doi.org/10.1515/chem-2021-0086
Salama SA, Al-Faifi ZE, El-Amier YA. Chemical composition of Reichardia tingitana methanolic extract and its potential antioxidant, antimicrobial, cytotoxic and larvicidal activity. Plants. 2022;11(15):2028. https://doi.org/10.3390/plants11152028
Boussaha S, Bramucci M, Rebbas K, Quassinti L, Mekkiou R, Maggi F. Chemical composition and anticancer activity of the essential oil from Vicia ochroleuca Ten., quite rare plant in Kabylia (Algeria). Nat Prod Res. 2023;2023:1-7. https://doi.org/10.1080/14786419.2023.2176492
Chowdhury RR, Ghosh SK. Phytol-derived novel isoprenoid immunostimulants. Front Immun. 2012;3:49. https://doi.org/10.3389/fimmu.2012.00049
Pejin B, Kojic V, Bogdanovic G. An insight into the cytotoxic activity of phytol at in vitro conditions. Nat Prod Res. 2014;28(22):2053-56. https://doi.org/10.1080/14786419.2014.921686
Sakthivel R, Malar DS, Devi KP. Phytol shows anti-angiogenic activity and induces apoptosis in A549 cells by depolarizing the mitochondrial membrane potential. Biomed Pharmacother. 2018;105:742-52. https://doi.org/10.1016/j.biopha.2018.06.035
Islam MT, Ali ES, Uddin SJ, Shaw S, Islam MA, Ahmed MI et al. Phytol: A review of biomedical activities. Food Chem Toxicol. 2018;121:82-94. https://doi.org/10.1016/j.fct.2018.08.032
Shariare MH, Noor HB, Khan JH, Uddin J, Ahamad SR, Altamimi MA et al. Liposomal drug delivery of Corchorus olitorius leaf extract containing phytol using design of experiment (DoE): In-vitro anticancer and in-vivo anti-inflammatory studies. Colloids Surf B. 2021;199:111543. https://doi.org/10.1016/j.colsurfb.2020.111543
de Alencar MVOB, Islam MT, da Mata AMOF, Dos Reis AC, de Lima RMT, de Oliveira Ferreira JR et al. Anticancer effects of phytol against Sarcoma (S-180) and Human Leukemic (HL-60) cancer cells. Environ Sci Pollut Res. 2023;30:80996-1007. https://doi.org/10.1007/s11356-023-28036-4
Song Y, Cui C, Zhu H, Li Q, Zhao F, Jin Y. Expression, purification and characterization of zinc-finger nuclease to knockout the goat beta-lactoglobulin gene. Protein Expr Purif. 2015;112:1-7. https://doi.org/10.1016/j.pep.2015.04.004
Armaka M, Papanikolaou E, Sivropoulou A, Arsenakis M. Antiviral properties of isoborneol, a potent inhibitor of herpes simplex virus type 1. Antiviral Res. 1999;43(2):79-92. https://doi.org/10.1016/S0166-3542(99)00036-4
Yang W, Chen X, Li Y, Guo S, Wang Z, Yu X. Advances in pharmacological activities of terpenoids. Nat Prod Commun. 2020;15(3):1934578X20903555. https://doi.org/10.1177/1934578X20903555
Kazi M, Alanazi Y, Kumar A, Shahba AAW, Rizwan Ahamad S, Alghamdi KM. Oral bioactive self-nanoemulsifying drug delivery systems of remdesivir and baricitinib: a paradigmatic case of drug repositioning for cancer management. Molecules. 2023;28(5):2237. https://doi.org/10.3390/molecules28052237
Bhardwaj M, Sali VK, Mani S, Vasanthi HR. Neophytadiene from Turbinaria ornata suppresses LPS-induced inflammatory response in RAW 264.7 macrophages and Sprague Dawley rats. Inflammation. 2020;43:937-50. https://doi.org/10.1007/s10753-020-01179-z
Gonzalez-Rivera ML, Barragan-Galvez JC, Gasca-Martínez D, Hidalgo-Figueroa S, Isiordia-Espinoza M, Alonso-Castro AJ. In vivo neuropharmacological effects of neophytadiene. Molecules. 2023;28(8):3457. https://doi.org/10.3390/molecules28083457
Selmy AH, Hegazy MM, El-Hela AA, Saleh AM, El-Hamouly MM. In vitro and in silico studies of Neophytadiene; a diterpene isolated from Aeschynomene elaphroxylon (Guill. &Perr.) Taub. as apoptotic inducer. Egypt J Chem. 2023. https://doi.org/10.21608/ejchem.2023.178261.7296
Fidyt K, Fiedorowicz A, Strz?da?a L, Szumny A. ?-caryophyllene and ?-caryophyllene oxide—natural compounds of anticancer and analgesic properties. Cancer Med. 2016;5(10):3007-17. https://doi.org/10.1002/cam4.816
Ambrož M, Šmatová M, Šadibolová M, Pospíšilová E, Hadravská P, Kašparová M et al. Sesquiterpenes ?-humulene and ?-caryophyllene oxide enhance the efficacy of 5-fluorouracil and oxaliplatin in colon cancer cells. Acta Pharm. 2019;69(1):121-28. https://doi.org/10.2478/acph-2019-0003
Patra JK, Das G, Bose S, Banerjee S, Vishnuprasad CN, del Pilar Rodriguez-Torres M et al. Star anise (Illicium verum): Chemical compounds, antiviral properties and clinical relevance. Phytother Res. 2020;34(6):1248-67. https://doi.org/10.1002/ptr.6614
Al-Owaisi M, Al-Hadiwi N, Khan SA. GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves. Asian Pac J Trop Biomed. 2014;4(12):964-70. https://doi.org/10.12980/APJTB.4.201414B295
Padmashree M, Roopa B, Ashwathanarayana R, Naika R. Antibacterial properties of Ipomoea staphylina Roem & Schult. plant extracts with comparing its preliminary qualitative phytochemical and quantitative GC-MS analysis. Trop Plant Res. 2018;5(3):349-69. https://doi.org/10.22271/tpr.2018.v5.i3.044
Ozcelik H, Tastan Y, Terzi E, Sonmez AY. Use of onion (Allium cepa) and garlic (Allium sativum) wastes for the prevention of fungal disease (Saprolegnia parasitica) on eggs of rainbow trout (Oncorhynchus mykiss). J Fish Dis. 2020;43(10):1325-30. https://doi.org/10.1111/jfd.13229
Syed DN, Khan N, Afaq F, Mukhtar H. Chemoprevention of prostate cancer through dietary agents: Progress and promise. Cancer Epidemiol Biomark Prev. 2007;16(11):2193-203. https://doi.org/10.1158/1055-9965.EPI-06-0942
Aron PM, Kennedy JA. Flavan-3-ols: Nature, occurrence and biological activity. Mol Nutr Food Res. 2008;52(1):79-104. https://doi.org/10.1002/mnfr.200700137
Seshadri VD, Vijayaraghavan P, Kim YO, Kim HJ, Al-Ghamdi AA, Elshikh MS et al. In vitro antioxidant and cytotoxic activities of polyherbal extracts from Vetiveria zizanioides, Trichosanthes cucumerina and Mollugo cerviana on HeLa and MCF-7 cell lines. Saudi J Biol Sci. 2020;27(6):1475-81. https://doi.org/10.1016/j.sjbs.2020.04.005
Kumar RS, Rajkapoor B, Perumal P. In vitro and in vivo anticancer activity of Indigofera cassioides Rottl. Ex. DC. Asian Pac J Trop Medi. 2011;4(5):379-85. https://doi.org/10.1016/S1995-7645(11)60108-9
Al-Amin M, Eltayeb NM, Khairuddean M, Salhimi SM. Bioactive chemical constituents from Curcuma caesia Roxb. rhizomes and inhibitory effect of curcuzederone on the migration of triple-negative breast cancer cell line MDA-MB-231. Nat Prod Res. 2021;35(18):3166-70. https://doi.org/10.1080/14786419.2019.1690489
Downloads
Published
Versions
- 05-01-2024 (2)
- 14-12-2023 (1)
How to Cite
Issue
Section
License
Copyright (c) 2022 Van Chen Tran, Minh-Nhut Truong, Thi Thuy Quynh Tran, Thanh To Nhi Nguyen, Hoang Khanh Linh Nguyen
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).