In vitro acute gamma radiation on tissue of pink and white lotus (Nelumbo nucifera Gaertn.) in Thailand
DOI:
https://doi.org/10.14719/pst.3005Keywords:
Acute gamma radiation, Embryo, LD50, Plant tissue culture, Pink lotus, WhitelotusAbstract
This research aimed to investigatethe age of embryos of pink and white lotus for in vitropropagation and examine the sensitivity of young lotus plants derived from tissue culture to acute gamma radiation. The seeds of both lotus varieties were 4-week, 6-week and 8-week-old embryos cultivated on solid MS medium. They were then transferred to solid MS medium supplemented with 3 mg/L BA. After culturing, the 6-week-old pink lotus embryos had an average maximum petiole length of 16.78 ± 1.01 cm and an average of 11.84 ± 0.44 roots/explant, while the 6-week-old white lotus embryos had an average maximum petiole length of 15.48 ± 0.68 cm and an average of 11.76 ± 0.70 roots/explant. Each young lotus plant produced one shoot. After treating the young lotus plants with liquid MS medium supplemented with 3 mg/L BA, both lotus varieties showed increased shoot numbers at 3.54 ± 0.13 and 3.40 ± 0.91 shoots/explant. Young lotus plants of both varieties were exposed to gamma radiation levels of 0, 20, 30 and 40 Gy. The LD50 value for pink lotus was 26.138 Gy, while the LD50 value for the white lotus was 32.031 Gy.The LD50 value will offer vital information for determining the optimal dosage of gamma radiation for both pink and white lotus in vitro.
Downloads
References
Lin Z, Zhang C, Cao D, Damaris RN, Yang P. The latest studies on lotus (Nelumbo nucifera). An emerging horticultural model plant. Int J Mol Sci. 2019;20(15):3680. https://doi:10.3390/ijms20153680
Hongpakdee P. Sacred lotus: Potential of thai flower through ASEAN market.Khon Kaen Agr J. 2013;41(3):213-20.
Thai crude drugs of the faculty of pharmacy.[Internet]. Ubon Ratchathani University; 2023. [cited 20 Aug 2023]. Available from: https://apps.phar.ubu.ac.th/thaicrudedrug/main. php?action=viewpage &pid=24
Yu X, Sheng J, Zhao L, Diao Y, Zheng X, Xie K, Hu Z.In vitro plant regeneration of lotus (Nelumbo nucifera). Open Life Sci. 2015;10(1):142-46. https://doi.org/10.1515/biol-2015-0016
Liu Q, Zhang D, Liu F, Qin M, Tian D. Micropropagation of Nelumbo nucifera ‘Weishan Hong’through germfree mature embryos. In Vitro Cell DevBiol. 2019;55:305-12. https://doi.org/10.1007/s11627-019-09984-4
Puripunyavanich V, Boonsirichai K. Induced mutation in yellow lotus by gamma irradiation. In: Proceedings of 44th Kasetsart University Annual Conference: Plants. 2006 30 Jan - 2 Feb; Thailand. Bangkok; Kasetsart University Library. 2006; p. 439-44.
Barampuram S, Allen G,Krasnyanski S.Effect of various sterilization procedures on the in vitro germination of cotton seeds.PCTOC. 2014;118:179-85.
https://doi.org/10.1007/s11240-014-0472-x
Yampul A, Dechkla M, Boonman N, Teerakathiti T, Phakpaknam P. Embryo of pink lotus and white lotus (Nelumbo nucifera Gaertn.) tissue culture and determination of seedling sensitivity to gamma rays. J Sci Technol MSU. 2020;39(5):506-13.
Deng X, Xiong Y, Li J, Yang D, Liu J, Sun Het al. The establishment of an efficient callus induction system for lotus (Nelumbo nucifera). Plants. 2020;9(11):1436. https://doi.org/ 10.3390/plants9111436
Noimai Y. Effects of plant growth regulators on seed germination treatment of Nymphaea gigantea ‘Atrans’ hybrid in vitroconditions. RMUTT Research Journal. 2018;17(2):54-64. https://doi.org/10.14456/rj-rmutt.2018.5
Wongpiyasatid A. Mutation: For plant breeding. Edition 1st. Bangkok: Kasetsart University Press. 2017;p. 297.
Padungsil N, Taechasinpitak T, Wongchowchan S, Chanchula C. Induction in Catharanthus roseus L. by acute gamma irradiation. tjst Journal. 2015;4(1):96-103.
https://doi.org/ 10.14456/tjst.2015.4
Soontornyatara S, Singhavorachai P, Puripunyavanich V, Taywiya P. Effect of gamma ray on morphological characteristics of Nelumbo nucifera (Roseum Plenum lotus). ActaHortic. 2017;1167:217-20. https://doi.org/10.17660/ActaHortic.2017.1167.33
Limsanguan P, Chanprame S, Ungvichian I, Srinamnguen P, Chanprame S. Gamma ray induced mutation in Chrysanthemum and detection of DNA polymorphism by AFLP. Agricultural Sci J. 2017;48(3):334-45.
Sherpa R, Devadas R, Bolbhat SN, Nikam TD, Penna S. Gamma radiation induced in-vitromutagenesis and isolation of mutants for early flowering and phytomorphological variations in Dendrobium 'Emma White'. Plants (Basel). 2022;11(22):3168.
https://doi: 10.3390/plants11223168
Pérez-Jiménez M, Tallón CI, Pérez-Tornero O. Inducing mutations in Citrus spp.: Sensitivity of different sources of plant material to gamma radiation. Appl Radiat Isot. 2020;157:109030. https://doi.org/10.1016/j.apradiso.2019.109030
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15:473-97.https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Firoz MA, Uddin ME, Amin R, Razzak MA, Manik MA, Khatun MM. Studies on the effect of various sterilization procedures for in vitro seed germination and successful micropropagation of Cucumis sativus. Int JPure Appl Biosci. 2016;4(1):75-81.
https://doi.org/10.18782/2320-7051.2226
Palee J. Natural propagation and micropropagation of Tupistra albiflora K. Larsen Sciand Tech RMUTT J. 2016;6(2):1-16.
Soliman AS, Abbas MS. Effects of sulfuric acid and hot water pre-treatments on seed germination and seedlings growth of Cassia fistula L. AEJAES. 2013;13(1):7-15. https://doi.org/10.5829/idosi.aejaes.2013. 13.01.1914
Shen-Miller J, Aung LH, Turek J, Schopf JW, Tholandi M, Yang M, Czaja A. Centuries-old viable fruit of sacred lotus Nelumbo nucifera Gaertn var. China antique. TropPlant Biol. 2013;6:53-68.https://doi.org/10.1007/s12042-013-9125-1
Trang NTQ, Hong HTK, Huong VTM, Long DT. In vitro propagation of red lotus (Nelumbo nucifera Gaertn). An aquatic edible plant in Vietnam. Agric SciDig. 2021;41(spl):129136. https://doi.org/10.18805/ag.D-257
ShouSY, Miao LX, Zai WS, Huang XZ, Guo DP. Factors influencing shoot multiplication of lotus (Nelumbo nucifera). Biol Plant. 2018;52:529-32.
https://doi.org/10.1007/s10535-008-0103-7
Beyaz R, Kahramanogullari CT, Yildiz C, Darcin ES, Yildiz M. The effect of gamma radiation on seed germination and seedling growth of Lathyrus chrysanthus Boiss. under in vitro conditions. J Environ Radio Act. 2016;162:129-33. https://doi.org/10.1016/j.jenvrad. 2016.05.006
Ferdous MH, Billah AM, Mehraj H, Taufique T, Uddin AFMJ. BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture. J Biosci Agric Res. 2015;3(2):87-95.https://doi.org/10.18801/jbar.030215.35
KhatoonS, Liu W, Ding CB, Liu X, Zheng Y, Zhang Yet al. In vitroevaluation of the effects of BAP concentration and pre-cooling treatments on morphological, physiological and biochemical traits of different olive (Olea euorpea L.) cultivars. Hortic. 2022;8(12):1108. https://doi.org/10.3390/horticulturae8121108
Buddharaksa P, Kaewdungtip C, U-Kong W. In vitro culture of Hippeastrum johnsonnii Bury and Caladium bicolor Vent. NUJST. 2011;19(1):18-23.
Ling WT, Liew FC, Lim WY, SubramaniamS, Chew BL. Shoot induction from axillary shoot tip explants of fig (Ficus carica) cv. Japanese BTM 6. Trop Life SciRes. 2018;29(2):165-74. https://doi.org/10.21315/tlsr2018. 29.2.11
Phakpaknam S, Yunchum N, Dechkla M, Pikunthong V.In vitro micropropagation and microrhizome induction of Curcuma comosaRoxb. RMUTP Research Journal. 2023;16(2):13-23. https://doi.org/10.14456/jrmutp.2022.18
Pikulthong V, Phakpaknam S, Dechkla M, Teerakathiti T.Optimal medium for watercress (Alternanthera sp.) micropropagation. SSSTJ. 2018;5(2):27-32.
Arunyanartl S, Soontronyatara S. Mutation induction by ? and X-ray irradiation in tissue cultured lotus. PCTOC. 2002;70:119-22.https://doi.org/10.1023/A:1016021627832
Kato A, Vega JM, Han F, Lamb JC, Birchler JA. Advances in plant chromosome identification and cytogenetic techniques. Curr Opin Plant Biol. 2005;8(2):148-54.https://doi.org/10.1016/j.pbi.2005.01.014
Takahashi N, Ogita N, Takahashi T, Taniguchi S, Tanaka M, Seki M, Umeda M. A regulatory module controlling stress-induced cell cycle arrest in Arabidopsis. Elife. 2019;8:e43944. https://doi.org/10.7554/eLife.43944
Hoda E. In vitroinduction of flower mutation in Catharanthus roseus using gamma irradiation. Alex Sci Exch. 2014;35:64-68.https://doi.org/10.21608/ASEJAIQJSAE.2014 .2575
Beyaz R. Impact of gamma irradiation pretreatment on the growth of common vetch (Vicia sativa L.) seedlings grown under salt and drought stress. Int J Radiat Biol. 2020;96(2):257-66.https://doi.org/10.1080/09553002.2020.1688885
Lee JW, Jo IH, Kim JU, Hong CE, Bang KH, Park YD. Determination of mutagenic sensitivity to gamma rays in ginseng (Panax ginseng) dehiscent seeds, roots and somatic embryos. Hortic Environ Biotechnol. 2019;60:721-31. https://doi.org/10.1007/s13580-019-00164-2
Pangjai W, Huehne SH. Gamma-ray induced morphological changes in rain lily (Zephyranthes spp.). Khon Kaen Agr J. 2021;49(3):643-55.
https://doi.org/10.14456/kaj. 2021.57.20
Hiswan J, Larekeng SH, Gusmiaty W, Putri SD. In vitro shoot induction of batik bamboo using BAP and NAA.Plant Arch. 2020;20(2):2343-49.
Piyachoteskulchai N. Effect of plant growth regulator on in vitro culture of Nelumbo nucifera Gaertn. Botany [thesis]. Bangkok (Thailand): Kasetsart University; 1996.
Downloads
Published
Versions
- 17-07-2024 (2)
- 15-07-2024 (1)
How to Cite
Issue
Section
License
Copyright (c) 2024 Vachiraporn Pikulthong, Nareerat Hongjan, Santi Ariya, Manussawee Dechkla, Narumon Boonman, Chanate Wanna, Piyada Wongwiwat, Sirirat Phakpaknam
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).