This is an outdated version published on 20-01-2024. Read the most recent version.
Forthcoming

Toxicity of Ocimum basilicum L. leaf extract against Spodoptera exigua Hübner (Lepidoptera: Noctuidae)

Authors

  • Nadya Sofia Siti Sa'adah Doctoral Program in Departement of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jln. Teknika Selatan, Sekip Utara, Sleman, 55281, Special Region of Yogyakarta, Indonesia https://orcid.org/0000-0003-1378-7212
  • Sukirno Sukirno Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jln. Teknika Selatan Sekip Utara Sleman, 55281 Yogyakarta, Indonesia. https://orcid.org/0000-0003-3708-9363
  • Tri Rini Nuringtyas Biochemistry Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jln. Teknika Selatan Sekip Utara Sleman, 55281 Yogyakarta, Indonesia https://orcid.org/0000-0002-8011-8396
  • Laurentius Hartanto Nugroho Plant Structure and Development Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jln. Teknika Selatan Sekip Utara Sleman , 55281 Yogyakarta, Indonesia https://orcid.org/0000-0001-7887-6860

DOI:

https://doi.org/10.14719/pst.3099

Keywords:

basil, beet armyworm, biopesticides, mortality

Abstract

The beet armyworm Spodoptera exigua Hübner (Lepidoptera: Noctuidae) is one of the most significant shallot pests worldwide, which affects agricultural production by approximately 100% in Indonesia. The growing awareness of sustainable agriculture encourages the search for natural alternatives to synthetic pesticides that promote resistance and contaminate the environment. Therefore, this study aims to evaluate the efficacy of basil (Ocimum basilicum L) leaf extract against the 2nd and 3rd instar larvae of S. exigua. The extracts were prepared using maceration and hydrodistillation and the pathogenicity was carried out against the 2nd and 3rd instar of S. exigua using three replicates with several doses of extract ranging from 0- 2.0%. The extracts were applied to the larvae using contact and topical methods. The results showed that maceration extraction yields a greater extract with different types compared to those of the hydrodistillation method. Maceration extract of basil leaves with the contact application method to 2nd instar showed better results than the topical application with the highest mortality rate of 75% (F=24.464; P< 0.001) and LC50 of 0.007%. This indicated that basil leaf extract with the contact application method has great potential to be developed as a botanical insecticide to control S. exigua in the field as part of Integrated Pest Management (IPM).

Downloads

Download data is not yet available.

References

Murcia-Meseguer A, Alves TJS, Budia F, Ortiz A, Medina P. Insecticidal toxicity of thirteen commercial plant essential oils against Spodoptera exigua (Lepidoptera: Noctuidae). Phytoparasitica. 2018;46(2):233–45. https://doi.org/10.1007/s12600-018-0655-9

Moulton JK, Pepper DA, Dennehy TJ. Beet armyworm ( Spodoptera exigua ) resistance to spinosad. Pest Management Science: formerly Pesticide Science. 2000;56(10):842-848. https://doi.org/10.1002/1526-4998(200010)56:10<842::AID-PS212>3.0.CO;2-H

Ahmad M, Arif MI. Resistance of beet armyworm Spodoptera exigua ( Lepidoptera?: Noctuidae ) to endosulfan, organophosphorus and pyrethroid insecticides in Pakistan. Crop Protection. 2010;29:1428–33. https://doi.org/10.1016/j.cropro.2010.07.025

Arulkumar G, Manisegaran S, Nalini R, Mathialagan M. Seasonable abundance of beet armyworm Spodoptera exigua ( Hubner ) infesting Onion with weather factors in Madurai district of Tamil Nadu. Journal of Entomology and Zoology Studies. 2017;5(6):1157–62.

Rauf A. Dinamika Populasi Spodoptera Exigua ( Hubner ) ( Lepidoptera?: Noctuidae ) pada Pertanaman Bawang Merah di Dataran Rendah. Buletin Hama dan Penyakit Tumbuhan. 1999;11(2):39–47.

Ujiyani F, Trisyono YA, Witjaksono W, Suputa S. Population of Spodoptera exigua Hübner during On- and Off-Season of Shallot in Bantul Regency, Yogyakarta. Jurnal Perlindungan Tanaman Indonesia. 2019;23(2):261. https://doi.org/10.22146/jpti.36740

Yooboon T, Pengsook A, Poonsri W, Pluempanupat W, Bullangpoti V. Toxicity of Phenylpropanoids from Alpinia galanga (Zingiberaceae) extracts against Spodoptera exigua Hübner (Lepidoptera: Noctuidae). Phytoparasitica. 2020;48(5):833–40. https://doi.org/10.1007/s12600-020-00830-7

Sukirno S, Tufail M, Rasool KG, El Salamouny S, Sutanto KD, Aldawood AS. The effectiveness of spinosad and neem extract against Spodoptera littoralis (Boisd.) and Spodoptera exigua (Hubner): Exploring possibilities to enhance the bio-pesticide persistence with natural UV protectants under field-sunlight conditions of Saudi Ara. Pakistan J Agric Sci. 2017;54(4):743–51.

Zheng S, Henken B, Wietsma W, Sofiari E, Jacobsen E, Krens FA, et al. Development of bio-assays and screening for resistance to beet armyworm (Spodoptera exigua Hubner) in Allium cepa L. and its wild relatives. Euphytica. 2000;114(1):77–85. https://doi.org/10.1023/A:1004089424419

Moekasan T, Basuki R. Status Resistensi Spodoptera exigua Hubn. Pada Tanaman Bawang Merah Asal Kabupaten Cirebon, Brebes, Dan Tegal Terhadap Insektisida Yang Umum Digunakan Petani Di Daerah Tersebut. Jurnal Hortikultura. 2007;17(4):83531.

Wibisono I, Trisyono YA, Martono E, Purwantoro A. Evaluasi resistensi terhadap metoksifenozida pada Spodoptera exigua di Jawa. Jurnal Perlindungan Tanaman Indonesia. 2007;13(2):127-35.

Senthil-Nathan S, Choi MY, Paik CH, Kalaivani K. The toxicity and physiological effect of goniothalamin, a styryl-pyrone, on the generalist herbivore, Spodoptera exigua Hübner. Chemosphere. 2008;72(9):1393–400. https://doi.org/10.1016/j.chemosphere.2008.03.037

Shan L, Chen L, Gao F, Zhou X. Diterpenoid alkaloids from Delphinium naviculare var. lasiocarpum with their antifeedant activity on Spodoptera exigua. Natural Product Research. 2019;33(22):3254–9. https://doi.org/10.1080/14786419.2018.1475382

Zhang JF, Chen L, Huang S, Shan LH, Gao F, Zhou XL. Diterpenoid Alkaloids from Two Aconitum Species with Antifeedant Activity against Spodoptera exigua. Journal of Natural Products. 2017;80(12):3136–42. https://doi.org/10.1021/acs.jnatprod.7b00380

Junhirun P, Pluempanupat W, Yooboon T, Ruttanaphan T, Koul O, Bullangpoti V. The Study of Isolated Alkane Compounds and Crude Extracts from Sphagneticola trilobata (Asterales: Asteraceae) as a Candidate Botanical Insecticide for Lepidopteran Larvae. Journal of Economic Entomology. 2018;111(6):2699–705. https://doi.org/10.1093/jee/toy246

Polato?lu K, Karakoç ÖC, Yücel Yücel Y, Gücel S, Demirci B, Demirci F, et al. Insecticidal activity of Salvia veneris Hedge. Essential oil against coleopteran stored product insects and Spodoptera exigua (Lepidoptera). Ind Crops Prod. 2017;97:93–100. https://doi.org/10.1016/j.indcrop.2016.12.012

Pandey AK, Singh P, Tripathi NN. Chemistry and bioactivities of essential oils of some Ocimum species: An overview. Asian Pac J Trop Biomed. 2014;4(9):682–94. https://doi.org/10.12980/APJTB.4.2014C77

Elumalai K, Krishnappa K, Anandan A, Govindarajan M, Mathivanan T. Larvicidal and ovicidal activity of seven essential oil against lepidopteran pest S. litura (Lepidoptera: Noctuidae). Int J Recent Sci Res. 2010;1:8–14.

Ruttanaphan T, Pluempanupat W, Aungsirisawat C, Boonyarit P, Goff G Le, Bullangpoti V, et al. Effect of Plant Essential Oils and Their Major Constituents on Cypermethrin Tolerance Associated Detoxification Enzyme Activities in Spodoptera litura (Lepidoptera: Noctuidae). J Econ Entomol. 2019;112(5):2167–76. https://doi.org/10.1093/jee/toz126

Sundararajan B, Moola AK, Vivek K, Kumari BDR. Formulation of nanoemulsion from leaves essential oil of Ocimum basilicum L. and its antibacterial, antioxidant and larvicidal activities (Culex quinquefasciatus). Microb Pathog. 2018;125(May):475–85. https://doi.org/10.1016/j.micpath.2018.10.017

Kannidi Siddhartha CC and MS. In vitro bioassay of certain botanical oils for their efficacy against maize fall army worm (J.E. Smith) Spodoptera frugiperda (Noctuidae: Lepidoptera). J Entomol Zool Stud 2. 2019;7(5):606–9.

Yarou BB, Bawin T, Boullis A, Heukin S, Lognay G, Verheggen FJ, et al. Oviposition deterrent activity of basil plants and their essentials oils against Tuta absoluta (Lepidoptera: Gelechiidae). Environ Sci Pollut Res. 2018;25(30):29880–8. https://doi.org/10.1007/s11356-017-9795-6

Marotti M, Piccaglia R, Giovanelli E. Differences in Essential Oil Composition of Basil (Ocimum basilicum L.) Italian Cultivars Related to Morphological Characteristics. J Agric Food Chem. 1996;44(12):3926–9. https://doi.org/10.1021/jf9601067

Shorey HH, Hale RL. Mass-Rearing of the Larvae of Nine Noctuid Species on a Simple Artificial Medium. J Econ Entomol. 1965;58(3):522–4. https://doi.org/10.1093/jee/58.3.522

Dougoud J, Toepfer S, Bateman M, Jenner WH. Efficacy of homemade botanical insecticides based on traditional knowledge. A review. Agron Sustain Dev. 2019;39(4).

Pavela R, Benelli G. Essential Oils as Ecofriendly Biopesticides? Challenges and Constraints. Trends Plant Sci [Internet]. 2016;21(12):1000–7. Available from: http://dx.doi.org/10.1016/j.tplants.2016.10.005

Zhang YN, He P, Xue JP, Guo Q, Zhu XY, Fang LP, et al. Insecticidal activities and biochemical properties of Pinellia ternata extracts against the beet armyworm Spodoptera exigua. J Asia Pac Entomol. 2017;20(2):469–76. https://doi.org/10.1016/j.aspen.2017.03.003

Ntalli N, Kopiczko A, Radtke K, Marciniak P, Rosinski G, Adamski Z. Biological activity of Melia azedarach extracts against Spodoptera exigua. Biol. 2014;69(11):1606–14. https://doi.org/10.2478/s11756-014-0454-9

Leatemia JA, Isman MB. Toxicity and antifeedant activity of crude seed extracts of Annona squamosa (Annonaceae) against lepidopteran pests and natural enemies. Int J Trop Insect Sci. 2004;24(2):150–8. https://doi.org/10.1079/IJT200416

Sa’adah NSS, Alwandri H, Nugroho LH, Sukirno S, Nuringtyas TR. A Meta-Analysis Study on Spodoptera exigua and Spodoptera litura Control: Biopesticides vs. Synthetic Pesticides . Proc 7th Int Conf Biol Sci (ICBS 2021). 2022;22(Icbs 2021):519–27. https://doi.org/10.2991/absr.k.220406.073

Leatemia JA, Isman MB. Insecticidal Activity of Crude Seed Extracts of Annona spp ., Lansium domesticum and Sandoricum koetjape Against Lepidopteran Larvae. 2004;32:30–7. https://doi.org/10.1007/BF02980856

Duarte JP, Redaelli LR, Silva CE, Jahnke SM. Effect of Azadirachta indica (Sapindales: Meliaceae) oil on the immune system of Spodoptera frugiperda (Lepidoptera: Noctuidae) immatures. J Insect Sci. 2020;20(3):1–6. https://doi.org/10.1016/j.bjp.2015.02.010

Saharkhiz MJ, Ghani A. Changes in Essential Oil Content and Composition of Clary Sage ( Salvia sclarea ) Aerial Parts during Different Phenological Stages. Medicinal and aromatic plant science and biotechnology. 2009;3(1): 90-93.

Anlas C, Ustuner O, Alkan FU, Bakirel T, Aydogan MN, Erel SB. A Comparative Study On The Antioxidant Activities And Phenolic Contents Of Different Extracts Of Achillea nobilis Subsp. Sipylea And Alcea apterocarpa (Fenzl) Boiss, Endemic Plants In Turkey. Fresenius Environmental Bulletin. 2017;26(2):1423–30.

da Silva Moura E, D’Antonino Faroni LR, Fernandes Heleno F, Aparecida Zinato Rodrigues A, Figueiredo Prates LH, Lopes Ribeiro de Queiroz ME. Optimal extraction of Ocimum basilicum essential oil by association of ultrasound and hydrodistillation and its potential as a biopesticide against a major stored grains pest. Molecules. 2020;25(12):2781. https://doi.org/10.3390/molecules25122781

Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Med (United Kingdom). 2018;13(1):1–26. https://doi.org/10.1186/s13020-018-0177-x

Ibrahim N, Abbas H, Sayed NS El, Gad HA. Rosmarinus officinalis L . hexane extract?: phytochemical analysis , nanoencapsulation , and in silico , in vitro , and in vivo anti ? photoaging potential evaluation. Sci Rep. 2022;1–20. https://doi.org/10.1038/s41598-022-16592-7

Regnault-Roger C, Vincent C, Arnason JT. Essential oils in insect control: low-risk products in a high-stakes world. Annual review of entomology. 2012;57:405-24.

Gökçe A, Whalon ME, Yanar Y. Archives Of Phytopathology And Plant Protection Contact and residual toxicities of 30 plant extracts to Colorado potato beetle larvae. Archives of Phytopathology and Plant Protection. 2007;40(6):37–41. https://doi.org/10.1080/03235400600628013

Kaleeswaran G, Firake DM, Sanjukta R, Behere GT, Ngachan S V. Bamboo-Leaf Prickly Ash extract: A potential bio-pesticide against oriental leaf worm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). J Environ Manage. 2018;208:46–55. https://doi.org/10.1016/j.jenvman.2017.12.017

Parsons GJ, Lees RS, Balaska S, Vontas J. A practical insecticide resistance monitoring bioassay for orally ingested dinotefuran in Anopheles malaria vectors. Insects. 2022 Mar 22;13(4):311. https://doi.org/10.3390/insects13040311

Mead HMI. Composition and Larvicidal Action of Ocimum basilicum L . Essential Oil against Spodoptera littoralis ( Boisd .). Journal of Plant Protection and Pathology. 2018;9(2):139–43. https://doi.org/10.21608/jppp.2018.41266

Kamaraj C, Rahuman AA, Bagavan A. Screening for antifeedant and larvicidal activity of plant extracts against Helicoverpa armigera (Hübner), Sylepta derogata (F.) and Anopheles stephensi (Liston). Parasitol Res. 2008;103(6):1361–8. https://doi.org/10.1007/s00436-008-1142-x

Lachowicz KJ, Jones GP, Briggs DR, Bienvenu FE, Palmer M V, Mishra V, et al. Characteristics of Plants and Plant Extracts from Five Varieties of Basil ( Ocimum basilicum L .) Grown in Australia. Journal of Agricultural and Food Chemistry. 1997;2660–5. https://doi.org/10.1021/jf960791h

Koul. Essential Oils as Green Pesticides: Potential and Constraints. Biopestic Int. 2008;4(1):63–84.

Mossa ATH. Green Pesticides: Essential oils as biopesticides in insect-pest management. J Environ Sci Technol. 2016;9(5):354–78. https://doi.org/10.3923/jest.2016.354.378

Nerio LS, Olivero-Verbel J, Stashenko E. Repellent activity of essential oils: A review. Bioresour Technol. 2010;101(1):372–8. https://doi.org/10.1016/j.biortech.2009.07.048

Campos EVR, Proença PLF, Oliveira JL, Pereira AES, De Morais Ribeiro LN, Fernandes FO, et al. Carvacrol and linalool co-loaded in ?-cyclodextrin-grafted chitosan nanoparticles as sustainable biopesticide aiming pest control. Sci Rep. 2018;8(1):1–14. https://doi.org/10.1038/s41598-018-26043-x

Draz KA, Tabikha RM, Eldosouky MI, Darwish AA, Abdelnasser M. Biotoxicity of essential oils and their nano-emulsions against the coleopteran stored product insect pests Sitophilus oryzae L. and Tribolium castaneum herbst. Int J Pest Manag. 2022;0(0):1–15. https://doi.org/10.1080/09670874.2022.2036862

Quassinti L, Bramucci M, Lupidi G, Barboni L, Ricciutelli M, Sagratini G, et al. In vitro biological activity of essential oils and isolated furanosesquiterpenes from the neglected vegetable Smyrnium olusatrum L. (Apiaceae). Food Chem. 2013;138(2–3):808–13. https://doi.org/10.1016/j.foodchem.2012.11.075

Rossi P, Cappelli A, Marinelli O, Valzano M, Pavoni L, Bonacucina G, et al. Mosquitocidal and anti-inflammatory properties of the essential oils obtained from monoecious, male, and female inflorescences of hemp (Cannabis sativa L.) and their encapsulation in nanoemulsions. Molecules. 2020;25(15): 3451. https://doi.org/10.3390/molecules25153451

Iason G. The role of plant secondary metabolites in mammalian herbivory: ecological perspectives. Proc Nutr Soc. 2005;64(1):123–31. https://doi.org/10.1079/PNS2004415

Hussain A, Rizwan-Ul-Haq M, AlJabr AM, Al-Ayedh H. Lethality of sesquiterpenes reprogramming red palm weevil detoxification mechanism for natural novel biopesticide development. Molecules. 2019;24(9):1–13. https://doi.org/10.3390/molecules24091648

Padilha De Paula J, Gomes-Carneiro MR, Paumgartten FJR. Chemical composition, toxicity and mosquito repellency of Ocimum selloi oil. J Ethnopharmacol. 2003;88(2–3):253–60. https://doi.org/10.1016/S0378-8741(03)00233-2

Deewatthanawong R, Kongchinda P, Deewatthanawong P, Pumnuan J, Insung A. GC-MS analysis and biopesticide properties of different crude extracts of Annona squamosa and Annona muricata. Int J Agric Technol. 2019;15(6):859–68.

Copping LG, Menn JJ. Biopesticides: A review of their action, applications and efficacy. Pest Manag Sci. 2000;56(8):651–76. https://doi.org/10.1002/1526-4998(200008)56:8<651::AID-PS201>3.0.CO;2-U

Manikanta penumudi, Dokuparthi sudheer kumar. A Review On Role Of Azadirachta indica A. Juss as a biopesticide. Int J Univers Pharm Bio Sci. 2014;3(2):10.

Published

20-01-2024

Versions

How to Cite

1.
Sofia Siti Sa’adah N, Sukirno S, Rini Nuringtyas T, Nugroho LH. Toxicity of Ocimum basilicum L. leaf extract against Spodoptera exigua Hübner (Lepidoptera: Noctuidae). Plant Sci. Today [Internet]. 2024 Jan. 20 [cited 2024 Nov. 5];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/3099

Issue

Section

Research Articles

Most read articles by the same author(s)