Exploring the therapeutic potential of ethanol extract of Erythrina fusca Lour. roots as an analgesic, antipyretic, and anti-inflammatory agent in experimental animals
DOI:
https://doi.org/10.14719/pst.3198Keywords:
analgesic effects, antipyretic properties, anti-inflammatory activity, therapeutic potentialAbstract
Erythrina fusca Lour., renowned in traditional medicine for its soothing, analgesic, anti-inflammatory, and neuroinhibitory properties, is the focus of our investigation. Expanding on its traditional applications, we assessed the analgesic, anti-inflammatory, and antipyretic characteristics of the ethanol extract from E. fusca root (REEF). Administered orally to mice at varying doses (50, 100, and 150 mg/kg for pain and fever reduction; 100, 150, and 200 mg/kg for anti-inflammatory experiments), REEF's effects were systematically evaluated through parameters such as reaction time, pain inhibition ratio, COX2 and PGE2 levels, rectal temperature, fever inhibition ratio, paw diameter, paw edema inhibition ratio, and inflammatory cytokines. Results reveal that REEF consistently reduced licking and biting time (p < 0.05) in response to formalin-induced pain in mice. Particularly noteworthy was the more pronounced reduction in COX2 and PGE2 during the late phase, with the most significant decrease observed at the 150 mg/kg REEF treatment (p < 0.05). The licking time graph substantiated the analgesic efficacy of REEF, illustrating simultaneous and sustained pain reduction over time. Moreover, REEF significantly alleviated fever (p < 0.05) in mice, with the most substantial effect observed three hours post-yeast injection. REEF not only efficiently reduced body temperature but also countered the fever-inducing effects of yeast at evaluated time points (p < 0.05). In the context of carrageenan-induced inflammation, paw swelling markedly diminished after 4 and 5 hours (p < 0.05). Notably, at the 5-hour mark, REEF treatment at 150 mg/kg dose exhibited significantly superior anti-inflammatory effects (p < 0.05). Additionally, mice treated with REEF (100, 150, and 200 mg/kg) exhibited a considerable decrease (p < 0.05) in serum concentrations of TNF-?, IL-1?, and IL-6 compared to the control treatment. The ethanol extract from Erythrina fusca roots demonstrates substantial potential for pain relief, fever reduction, and anti-inflammatory effects, positioning it as a promising herbal resource for the research and development of natural medicinal products with these therapeutic properties.
Downloads
References
Noor F, Rupak MAHB, Chowdhury MM, Shurovi FS, Sarif S, Hasan MM, Ferdous J, Tahsin MR, Chowdhury JA, Kabir S, Chowdhury AA, Aktar F, Amran MS, Akter T. An evaluation of analgesic and anti-inflammatory activity of ethanolic extract of Cynodon dactylon on stressed rodent model. Biomedical Journal of Scientific & Technical Research. 2022;42(3):33550-57. http://doi.org/10.26717/BJSTR.2022.42.006741
Wylezinski LS, Gray JD, Polk JB, Harmata AJ, III CFS. Illuminating an invisible epidemic: a systemic review of the clinical and economic benefits of early diagnosis and treatment in inflammatory disease and related syndromes. Journal of Clinical Medicine. 2019;8(4):493. http://doi.org/10.3390/jcm8040493
Vonkeman HE, Van de Laar MAFJ. Nonsteroidal anti-inflammatory drugs: adverse effects and their prevention. Seminars in Arthritis and Rheumatism. 2010;39(4):294-312. http://doi.org/10.1016/j.semarthrit.2008.08.001
Benyamin R, Trescot AM, Datta S, Buenaventura R, Adlaka R, Sehgal N, Glaser SE, Vallejo R. Opioid complications and side effects. Pain Physician. 2008;11(2):105-20.
Jiménez-Cabrera T, Bautista M, Velázquez-González C, Jaramillo-Morales OA, Guerrero-Solano JA, Urrutia-Hernández TA, O-Arciniega MD. Promising antioxidant activity of erythrina genus: an alternative treatment for inflammatory pain? International Journal of Molecular Sciences. 2021;22(1):248. http://doi.org/10.3390/ijms22010248
Sapura A, Amini MH, Farhan MI, Azman MFSN, Salim F. Antibacterial activity and phytochemical screening of Erythrina fusca Lour. leaf extract (Fabaceae). Science Letters. 2022;16(1):60-71. http://doi.org/10.24191/sl.v16i1.15276
Anjum A, Sultan MZ, Ferdosh S, Islam MK, Rashid MA, Nahar L, Sarker SD. Flavonoid, pterocarpans and steroid from Erythrina fusca Lour. growing in Bangladesh: isolation, and antimicrobial and free-radical scavenging activity. Journal of Medicinal Plants. 2021;20(79):37-46.
Tran TPN, Nguyen TT, Tran GB. Anti-arthritis effect of ethanol extract of sacha inchi (Plukenetia volubilis L.) leaves against complete Freund’s adjuvant-induced arthritis model in mice. Tropical Life Sciences Research, 202334(3):237-57. https://doi.org/10.21315/tlsr2023.34.3.13
Alison A. Basel Declaration defends animal research. Nature. 2010; 468:742.
Nhung TNP, Quoc LPT. Analgesic and antipyretic activities of ethanol extract of Gardenia jasminoides Ellis fruits in mice. Tropical Journal of Natural Product Research. 2023;7(10):4902-07. http://doi.org/10.26538/tjnpr/v7i10.27
Bhowmick R, Sarwa MS, Dewan SMR, Das A, Das B, Uddin MMN, Islam MS, Islam MS. In vivo, analgesic, antipyretic, and anti-inflammatory potential in Swiss albino mice and in vitro thrombolytic activity of hydroalcoholic extract from Litsea glutinosa leaves. Biological Research. 2014;47:56. http://doi.org/10.1186/0717-6287-47-56
Azmi AS, Rahim NA, Zahari Z, Salim F. Cytotoxic activity of Erythrina fusca Lour. leaf, twig, and flower extracts. Malaysian Journal of Analytical Sciences. 2020;24(3):313-19.
Rajeev VE, Latha PG, Anuja GI, Suja SR, Sini S, Shine VJ, Shyamal S, Krishnakumar NM, Shikha P, Liji J, Rajasekharan S. Antiinflammatory and analgesic properties of Erythrina indica Lamb. Medicinal Plants - International Journal of Phytomedicines and Related Industries. 2010;2(3):229-231. http://doi.org/10.5958/j.0975-4261.2.3.036
Murugalakshmi M, Mari Selvi J, Thangapandian.V. Analgesic, antipyretic and anti-inflammatory activities of Erythrina variegata leaves extracts. Journal of Advanced Botany and Zoology. 2014;2(2):1-5.
Berkan T, Ustünes L, Lermioglu F, Ozer A. Antiinflammatory, analgesic, and antipyretic effects of an aqueous extract of Erythraea centaurium. Planta Medica. 1991;57(1):34-37. http://doi.org/10.1055/s-2006-960011
Mohammed MS, Osman WJA, Garelnabi EAE, Osman Z, Osman B, Khalid HS, Mohamed MA. Secondary metabolites as anti-inflammatory agents. Journal of Phytopharmacology. 2014;3(4):275-285.
Al-Snafi AE. Medicinal plants possessed anti-inflammatory antipyretic and analgesic activities (part 2) - plant-based review. Scholars Academic Journal of Pharmacy. 2016;5(5):142-158.
López-Cano M, Fernández-Dueñas V, Llebaria A, Ciruela F. Formalin murine model of pain. Bio-Protocol. 2017;7(23):2628. http://doi.org/10.21769/BioProtoc.2628
Hunter D, Chai C, Barr GA. Effects of COX inhibition and LPS on formalin-induced pain in the infant rat. Developmental Neurobiology. 2015;75(10):1068-1079. http://doi.org/10.1002/dneu.22230
Park J, Lee C, Kim YT. Effects of natural product-derived compounds on inflammatory pain via regulation of microglial activation. Pharmaceuticals. 2023;16(7):941. https://doi.org/10.3390/ph16070941
Kundu P, Debnath SL, Devnath HS, Saha L, Sadhu SK. Analgesic, anti-inflammatory, antipyretic, and in silico measurements of Sonneratia caseolaris (L.) fruits from Sundarbans, Bangladesh. BioMed Research International. 2022;2022:1405821. https://doi.org/10.1155/2022/1405821
Subhawa S, Arpornchayanon W, Jaijoy K, Chansakaow S, Soonthornchareonnon N, Sireratawon S. Anti-inflammatory, antinociceptive, antipyretic, and gastroprotective effects of Eurycoma longifolia jack ethanolic extract. Life. 2023;13(7):1465. https://doi.org/10.3390/life13071465
Asefa M, Teshome N, Degu A. Anti-inflammatory and analgesic activity of methanolic root extract of Verbascum sinaiticum Benth. Journal of Inflammation Research. 2022;15:6381-6392. http://doi.org/10.2147/JIR.S389430
Gou KJ, Zeng R, Dong Y, Hu QQ, Hu HWY, Maffucci KG, Dou QL, Yang QB, Qin XH, Qu Y. Anti-inflammatory and analgesic effects of Polygonum orientale L. extracts. Frontiers in Pharmacology. 2017;8:562. https://doi.org/10.3389/fphar.2017.00562
Downloads
Published
Versions
- 29-06-2024 (2)
- 25-06-2024 (1)
How to Cite
Issue
Section
License
Copyright (c) 2024 Tran Thi Phuong Nhung, Le Pham Tan Quoc
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).