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Antioxidant analysis of protein from fresh and dry leaf of Orthosiphon aristatus (Blume) Miq.

Authors

  • Heeravathy Ramachandran Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia https://orcid.org/0000-0002-7495-4582
  • Zaidah Rahmat Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia; Innovation Centre in Agritechnology for Advanced Bioprocessing (ICA), Universiti Teknologi Malaysia Pagoh, 84600 Pagoh, Johor, Malaysia ;Chemical Management Unit, Universiy Laboratory Management Centre, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia https://orcid.org/0000-0002-5692-3790

DOI:

https://doi.org/10.14719/pst.3274

Keywords:

Orthosiphon aristatus, electrophoresis, protein, antioxidant, DPPH, FRAP

Abstract

Orthosiphon aristatus (Blume) Miq. is a folklore plant consumed as brewed tea for various health benefits. The white variety of the plants leaf and stem are predominantly sold in the market in dried form, blooming as a natural herbal product. To date, no proteomics and antioxidant studies are available on the fresh and dried leaf protein extract of O. aristatus since most studies take an interest in the crude extracts of plants. Thereupon, this study focuses on the One-dimensional (1D) electrophoretic pattern of the fresh and dry leaf determined via sodium dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Additionally, antioxidant proteins are currently being focused on for their link to controlling disease through their potential to destroy free radicals that are present in excess. Hence, an antioxidant assay was conducted for the fresh and dry leaf protein extract using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical assay and ferric reducing antioxidant power (FRAP) assay. The electrophoretic pattern obtained resulted in approximately 16 and 10 bands for the fresh and dry leaves respectively. As for the antioxidant activity, the dry leaf had higher radical scavenging activity and higher reducing power compared to the fresh leaf. The findings obtained provides insight in to  the protein content and antioxidant activity found in the protein extract of fresh and dry leaves of O. aristatus. Hence, further detailed study of the protein extracts could further unveil their therapeutic potential.

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Published

30-09-2024

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How to Cite

1.
Ramachandran H, Rahmat Z. Antioxidant analysis of protein from fresh and dry leaf of Orthosiphon aristatus (Blume) Miq. . Plant Sci. Today [Internet]. 2024 Sep. 30 [cited 2024 Nov. 4];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/3274

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