Evaluation of Anti-angiogenic Activity of Iraqi Mesembryanthemum cordifolium L. f. using rat aorta ring assay

Zahraa Jasim Abed; Amjed Haseeb Khamees

doi:10.14719/pst.3402

Authors

Zahraa Jasim Abed Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Baghdad, Baghdad, Iraq https://orcid.org/0009-0003-9303-3986
Amjed Haseeb Khamees Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Baghdad, Baghdad, Iraq https://orcid.org/0000-0001-9044-3769

DOI:

https://doi.org/10.14719/pst.3402

Keywords:

Angiogenesis, chloroform, Mesembryanthemum cordifolium, phytochemical analysis

Abstract

New blood vessels are produced during angiogenesis, which is necessary in both normal and abnormal physiological and pathologic states. The use of non-toxic plant extracts that inhibit angiogenesis might lead to more focus on the use of natural sources as a pivotal therapeutic agent in the treatment of many diseases and malignancies. Mesembryanthemum cordifolium L. f. is belongs to the Aizoaceae family and commonly known as the Heartleaf Ice Plant or Tiny Sun Rose. The plant was cultivated in the city of al Diwaniyah. No previous research has been conducted on this plant and its antiangiogenic properties. Hence, this research aimed to assess the antiangiogenic capacity of the Iraqi M. cordifolium. For this study, male albino rats aged 12 to 14 weeks were used. With a Soxhlet apparatus, the defatted plant components were extracted in 85 % methanol for 12 h. Subsequently, each fraction was fractionated using petroleum ether, chloroform and ethyl acetate and each fraction underwent phytochemical analysis. Rat aortic rings were infused with 100 ?g/mL of each plant component and placed in a growth medium. In addition, the dose-response relationship was assessed for the most active fraction at 6 different concentrations ranging from 6.25 ?g/mL to 100 ?g/mL. Compared to the negative control (1 % DMSO), the results demonstrated that fractions of M. cordifolium inhibit the growth of blood vessels in a dose-dependent manner; chloroform demonstrates the most potent effect. The results indicate that the chloroform fraction derived from M. cordifolium has a good ability to limit blood vessel development. This makes it a viable option for future investigation as an angiogenesis inhibitor.

Downloads

Download data is not yet available.

References

Lee JH, Nam SY. Comparison of growth and leaf color quality of Mesembryanthemum cordifolium f. variegata as affected by shading levels. Journal of People, Plants and Environment. 2023 Jun 1;26(3):207-17. https://doi.org/10.11628/ksppe.2023.26.3.207

Waltenberger J, Mayr U, Frank H, Hombach V. Suramin is a potent inhibitor of vascular endothelial growth factor. A contribution to the molecular basis of its antiangiogenic action. J Mol Cell Cardiol. 1996;28(7):1523-29. https://doi.org/10.1006/jmcc.1996.0142

Kreimeyer A, Müller G, Kassack M, Nickel P, Gagliardi ART. Suramin analogues with a 2-phenylbenzimidazole moiety as partial structure; potential anti HIV- and angiostatic drugs, 2: Sulfanilic acid, benzenedisulfonic acid and naphthalene trisulfonic acid analogues. Arch Pharm (Weinheim) [Internet]. 1998 Mar 1 [cited 2024 Feb 15];331(3):97-103. Available from: https://europepmc.org/article/MED/9557135

Qassim RH, Kadhem EJ. Phytochemical investigation and anti-angiogenic examination of Iraqi Vigna radiata L. seeds and sprouts. Iraqi Journal of Pharmaceutical Sciences. 2020 Dec 27;29(2):37-47. https://doi.org/10.31351/vol29iss2pp37-47

Carmeliet P. Angiogenesis in life, disease and medicine. Nature [Internet]. 2005 Dec 15 [cited 2024 Feb 15];438(7070):932-36. Available from: https://pubmed.ncbi.nlm.nih.gov/16355210/

Khamees A, Khadim E, Sahib H. Investigation of the possible anti-angiogenic activity of Iraqi Scabiosa palaestina L. using ex vivo rat aorta ring assay. Journal of Complementary Medicine Research. 2021;12(4):249. https://doi.org/10.5455/jcmr.2021.12.04.37

Haseeb Khamees A, Jawad Kadheem E, Bahaa Sahib H, Hussein Ahmed O. A review on medicinal plants with antiangiogenic activity available in Iraq. J Pharm Res Int. 2019 Nov 30;1-10. https://doi.org/10.9734/jpri/2019/v31i630331

Khamees AH, Abdulhussein AJ, Sahib HB, Fawzi HA. Anti-angiogenic and antioxidant activity of Iraqi Cyperus rotundus ethanol extract. International Journal of Pharmacology. 2018;14(4):546-52. https://doi.org/10.3923/ijp.2018.546.552

Petrovic P. Targeting angiogenesis in cancer treatments: Where do we stand ? Journal of Pharmacy and Pharmaceutical Sciences [Internet]. 2016 Jun 26 [cited 2024 Feb 16];19(2):226-38. Available from: https://pubmed.ncbi.nlm.nih.gov/27518172/

View of A pharmacognostic approach, including phytochemical and GC-MS analysis, targeted towards the authentication of Strobilanthes jomyi P. Biju, Josekutty, Rekha & J.R.I.Wood [Internet]. [cited 2024 Mar 4]. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2104/1916

View of phytochemical screening and gas chromatography-mass spectrometry analysis on Ischaemum pilosum Kleinex Willd. [Internet]. [cited 2024 Mar 4]. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2349/2422

View of investigation of phytochemical constituents, GC-MS, DPPH free radical scavenging assay and mineral contents of Glochidion sphaerogynum (Mull. Arg.) Kurz bark extract [Internet]. [cited 2024 Mar 4]. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2019/1879

Khamees AH, Kadhim EJ. Isolation, characterization and quantification of a pentacyclic triterpinoid compound ursolic acid in Scabiosa palaestina L. distributed in the North of Iraq. Plant Science Today [Internet]. 2022 Jan 1 [cited 2024 Feb 14];9(1):178-82. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1398

Brahma S, Mochahary B, Kalita M, Goyal AK. Pharmacognostic and physicochemical characterisation of potential plants for antidiabetic herbal formulations. Plant Science Today [Internet]. 2022 Aug 3 [cited 2024 Mar 4];9(sp2):1-7. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1704

View of phytochemicals analysis and antioxidant potential of hydroalcoholic extracts of fresh fruits of Pistacia atlantica and Pistacia khinjuk [Internet]. [cited 2024 Mar 4]. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2853/2776

Brown KJ, Maynes SF, Bezos A, Maguire DJ, Ford MD, Parish CR. A novel in vitro assay for human angiogenesis. Lab Invest [Internet]. 1996 Oct 1 [cited 2024 Feb 16];75(4):539-55. Available from: https://europepmc.org/article/MED/8874385

Zhu WH, Guo X, Villaschi S, Nicosia RF. Regulation of vascular growth and regression by matrix metalloproteinases in the rat aorta model of angiogenesis. Lab Invest [Internet]. 2000 [cited 2024 Feb 16];80(4):545-55. Available from: https://pubmed.ncbi.nlm.nih.gov/10780671/

Sahib HB. The anti-angiogenic and anti-proliferative activity of methyl hydroxychalcone. Asian Pacific Journal of Cancer Prevention. 2022;23(6):2071-77. https://doi.org/10.31557/APJCP.2022.23.6.2071

Nicosia RF, Lin YJ, Hazelton D, Qian XH. Endogenous regulation of angiogenesis in the rat aorta model. Role of vascular endothelial growth factor. Am J Pathol [Internet]. 1997 Nov 1 [cited 2024 Feb 16];151(5):1379-86. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/9358764/?tool=EBI

Ali ZK, Sahib HB. Antiangiogenic activity of sweet almond (Prunus dulcis) oil alone and in combination with aspirin in both in vivo and in vitro assays. Asian Pacific Journal of Cancer Prevention. 2022 Apr 1;23(4):1405-13. https://doi.org/10.31557/APJCP.2022.23.4.1405

Nasim N, Sandeep IS, Mohanty S. Plant-derived natural products for drug discovery: current approaches and prospects. The Nucleus [Internet]. 2022 Dec 1 [cited 2024 Mar 2];65(3):399. Available from: /pmc/articles/PMC9579558/

Alasvand M, Assadollahi V, Ambra R, Hedayati E, Kooti W, Peluso I. Antiangiogenic effect of alkaloids. Oxid Med Cell Longev. 2019;2019. https://doi.org/10.1155/2019/9475908

Thawabteh A, Juma S, Bader M, Karaman D, Scrano L, Bufo SA et al. The biological activity of natural alkaloids against herbivores, cancerous cells and pathogens. Toxins (Basel). 2019 Nov 11;11(11). https://doi.org/10.3390/toxins11110656

Meyers MO, Gagliardi AR, Flattmann GJ, Su JL, Wang YZ, Woltering EA. Suramin analogs inhibit human angiogenesis in vitro. Journal of Surgical Research. 2000 Jun 15;91(2):130-34. https://doi.org/10.1006/jsre.2000.5920

Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. J Ethnopharmacol. 2005 Aug 22;100(1–2):72-79. https://doi.org/10.1016/j.jep.2005.05.011

Wang S, Zheng Z, Weng Y, Yu Y, Zhang D, Fan W et al. Angiogenesis and anti-angiogenesis activity of Chinese medicinal herbal extracts. Life Sci. 2004 Apr 2;74(20):2467-78. https://doi.org/10.1016/j.lfs.2003.03.005

Sharifi-Rad J, Dey A, Koirala N, Shaheen S, El Omari N, Salehi B et al. Cinnamomum species: Bridging phytochemistry knowledge, pharmacological properties and toxicological safety for health benefits. Front Pharmacol [Internet]. 2021 May 11 [cited 2024 Mar 3];12:600139. https://doi.org/10.3389/fphar.2021.600139

Mondal A, Gandhi A, Fimognari C, Atanasov AG, Bishayee A. Alkaloids for cancer prevention and therapy: Current progress and future perspectives. Eur J Pharmacol. 2019 Sep 5;858:172472. https://doi.org/10.1016/j.ejphar.2019.172472

Said A, Attia E, Abdelmohsen U, A Fouad M. Natural products potential of the genus Aptenia. Journal of Advanced Biomedical and Pharmaceutical Sciences. 2019 Mar 5;0(0):0-0. https://doi.org/10.21608/jabps.2019.6908.1032

Waweru W, Wambugu FK, Mbabazi R. Evaluation of anti-inflammatory activity of Aptenia cordifolia leaves extract in wistar albino rats. J Pharmacogn Phytochem. 2017;

Tang J, Feng Y, Tsao S, Wang N, Curtain R, Wang Y. Berberine and coptidis rhizoma as novel antineoplastic agents: A review of traditional use and biomedical investigations. J Ethnopharmacol [Internet]. 2009 Oct 29 [cited 2024 Feb 16];126(1):5-17. Available from: https://pubmed.ncbi.nlm.nih.gov/19686830/