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Plant Science Today (PST)

Research Articles

Vol. 12 No. 2 (2025)

Effect of different concentrations of 2,4-D and BAP on callus and shoots induction of Thai Cannabis (Cannabis sativa L.) cv. Hang Kra Rog Phu Phan

DOI
https://doi.org/10.14719/pst.3687
Submitted
10 April 2024
Published
15-04-2025 — Updated on 28-04-2025
Versions

Abstract

The Thai Cannabis cultivar (Cannabis sativa L.) cv. Hang Kra Rog Phu Phan is highly demanded in the pharmaceutical, cosmetic and various industries. This study investigates the effects of different concentrations of 2,4-D on callogenesis and BAP on shoot multiplication to enhance the propagation of this valuable cultivar. The results show that the MS medium supplemented with 3 mg/L of 2,4-D induces the highest callogenesis, while the MS medium supplemented with 3 mg/L BAP promotes the maximum shoot multiplication. These findings have the potential to significantly impact the cultivation of the Thai Cannabis cultivar "Hang Kra Rog Phu Phan," ensuring a sufficient supply to meet the increasing demand in various applications.

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