Skip to main navigation menu Skip to main content Skip to site footer
Plant Science Today (PST)

Research Articles

Vol. 11 No. 4 (2024)

Combating tobacco caterpillar, Spodoptera litura (Fab.) through isolated strains of Streptomyces from the soils of Kerala and Tamil Nadu

DOI
https://doi.org/10.14719/pst.3748
Submitted
22 April 2024
Published
21-12-2024 — Updated on 23-12-2024
Versions

Abstract

The tobacco caterpillar, Spodoptera litura (Fab.) is a polyphagous pest on agricultural and horticultural crops. Biological control is an effective and sustainable alternative to insecticides for insect pest management. Now-a-days, microbial compounds are used for the management of insect pests and they are the good alternative to inorganic pesticides. The various microbial control agents are obtained from the different microorganisms, actinomycetes specifically the filamentous Streptomyces produce metabolites possessing insecticidal activity. The Streptomyces is a Gram-positive multicellular bacteria and they possess numerous secondary active metabolites. The soil samples were collected from different locations in Kerala and Tamil Nadu states, India. Isolation of Streptomyces was carried out by serial dilution of 10-4 and pour plating method. The Streptomyces can be cultured in International Streptomyces Project 2 fermentation broth. The Streptomyces sp. such as S. katrae (ST 1), S. acidiscabies (ST 3), S. andamanensis (ST 5) and S. cerasinus (ST 7) were isolated from the soils of Kerala and Tamil Nadu, India by 16S rDNA sequencing and matched with Streptomyces sp. using NCBI BLAST program and were screened against 1st and 2nd instar larvae of S. litura. The strain S. katrae (ST 1) and a consortium of all the strains (ST 1 + ST 3 + ST 5 + ST 7) exhibited 66 - 70 % mortality of 1st instar larvae and 70-74 % mortality of 2nd instar larvae of S. litura. The presence of secondary active metabolites in the ISP 2 fermentation broth exhibited strong larvicidal activities and these findings indicate that the ISP 2 fermentation broth of S. katrae and the consortium possess the ability to control the pest populations at the desirable level.

References

  1. Ashok K, Pavithran S. Biology and morphometrics of Spodoptera litura (Fab.) on castor. 2022;456-58. https://doi.org/10.55446/IJE.2021.261.
  2. Armes NJ, Wightman JA, Jadhav DR, Ranga Rao GV. Status of insecticide resistance in Spodoptera litura in Andhra Pradesh, India. Pesticide Science. 1997;50(3):240-48. https://doi.org/10.1002/(SICI)10969063(199707)50:3%3C240::AID
  3. Yadav D, Kamte A, Jadhav R. Bio-efficacy of cyantraniliprole, a new molecule against Scelodonta strigicollis Motschulsky and Spodoptera litura Fabricius in grapes. Pest Management in Horticultural Ecosystems. 2012;18(2):128-34. https://api.semanticscholar.org/CorpusID:76651763.
  4. Chari M, Bharpoda T, Patel A. Bioefficacy of fluvalinate against Spodoptera litura in tobacco nursery. Pestology. 1986;10(1):21-24. https://www.researchgate.net/publication/328419688.
  5. Patil, Ranjeet A, Deepak M, Mehta, Babu Lal Jat. Studies on life fecundity tables of Spodoptera litura Fabricius on tobacco Nicotiana tabacum Linnaeus. Entomol Ornithol Herpetol. 2014;3:1000118. https://doi.org/10.4172/2161-0983.1000118.
  6. Latha M, Shivanna B, Manjunatha M, Kumaraswamy M. Biology of Spodoptera litura on chewing tobacco in vitro. Journal of Eco-friendly Agriculture. 2014;9(1):43-47. https://doi.org/10.14719/pst.3078.
  7. Sharma HC. Climate change effects on insects: implications for crop protection and food security. Journal of Crop Improvement. 2014;28(2):229-59. https://www.researchgate.net/publication/263611092.
  8. Kutovaya OA, Watson SB. Development and application of a molecular assay to detect and monitor geosmin-producing cyanobacteria and actinomycetes in the Great Lakes. Journal of Great Lakes Research. 2014;40(2):404-14. https://www.researchgate.net/publication/261998971.
  9. Hwang BK, Ahn SJ, Moon SS. Production, purification and antifungal activity of the antibiotic nucleoside, tubercidin produced by Streptomyces violaceoniger. Canadian Journal of Botany. 1994;72(4):480-85. https://www.researchgate.net/publication/237165270.
  10. Arasu MV, Al-Dhabi NA, Saritha V, Duraipandiyan V, Muthukumar C, Kim SJ. Antifeedant, larvicidal and growth inhibitory bioactivities of novel polyketide metabolite isolated from Streptomyces sp. AP-123 against Helicoverpa armigera and Spodoptera litura. BMC Microbiology. 2013;131:6. https://www.researchgate.net/publication/236738655.
  11. Montesinos E. Development, registration and commercialization of microbial pesticides for plant protection. International Microbiology. 2003;6:245-52. https://www.researchgate.net/publication/10582115.
  12. Saljoqi AUR, Khan J, Ali G. Rearing of Spodoptera litura (Fabricius) on different artificial diets and its parasitization with Trichogramma chilonis (Ishii). Pakistan Journal of Zoology. 2015;47(1). https://cabidigitallibrary.orgby2409:4073:310:c362:38b9:7118:c993:8ee5.
  13. Vijayabharathi R, Kumari BR, Sathya A, Srinivas V, Abhishek R, Sharma HC, Gopalakrishnan S. Biological activity of entomopathogenic actinomycetes against lepidopteran insects (Noctuidae: Lepidoptera). Canadian Journal of Plant Science. 2014;94(4):759-69. https://doi.org/10.4141/cjps2013-298.
  14. Abbott WS. A method of computing the effectiveness of an insecticide. The Journal of Economic Entomology. 1925;18(2):265-67. https://pubmed.ncbi.nlm.nih.gov/3333059/.
  15. Gomez KA, Gomez AA. Statistical procedures for agricultural research: John Wiley and Sons. 1984.
  16. Kaur T, Manhas RK. Antifungal, insecticidal and plant growth promoting potential of Streptomyces hydrogenans DH16. Journal of Basic Microbiology. 2014;54(11):1175-85. https://www.researchgate.net/publication/237822910.
  17. Valanarasu M, Kannan P, Ezhilvendan S, Ganesan G, Ignacimuthu S, Agastian P. Antifungal and antifeedant activities of extracellular product of Streptomyces spp. ERI-04 isolated from Western Ghats of Tamil Nadu. Journal de Mycologie Medicale. 2010;20(4):290-97. https://www.researchgate.net/publication/260291397.
  18. Sathya A, Vijayabharathi R, Kumari BR, Srinivas V, Sharma HC, Sathyadevi P, Gopalakrishnan S. Assessment of a diketopiperazine, cyclo (Trp-Phe) from Streptomyces griseoplanus SAI-25 against cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae). Applied Entomology and Zoology. 2016;51:11-20. http://dx.doi.org/10.1007/s13355-015-0366-3.
  19. Dhar A. Plant protection practices by using botanicals for sustainable agriculture. Plant Archives. 2020;20(2):3741-46. https://doi.org/10.4081/ija.2021.1851.
  20. Prakash VA, Sermalatha G, Selvarathinam T. Extraction of bioactive compounds from Streptomyces avermitilis and Azadirachta indica and evaluation against Spodoptera litura: A green approach. Journal of Entomology and Zoology Studies. 2022;10(1):143-52. https://www.researchgate.net/publication/358118270.

Downloads

Download data is not yet available.