Skip to main navigation menu Skip to main content Skip to site footer
Plant Science Today (PST)

Research Articles

Vol. 11 No. 4 (2024)

Marker-assisted introgression of a major broad spectrum blast resistance gene Pi54 into a popular rice variety Warangal Samba (WGL-14)

DOI
https://doi.org/10.14719/pst.4066
Submitted
7 June 2024
Published
29-11-2024 — Updated on 04-12-2024
Versions

Abstract

Warangal Samba (WGL-14) is a widely known medium slender-grain rice variety that matures within 135-140 days. This variety has been extensively cultivated in Telangana, India, due to its high yield potential of 7-7.5 t/ha and its favourable cooking qualities. However, it is highly susceptible to rice blast disease caused by the fungus Magnaporthe oryzae. This research sought to improve the blast resistance of WGL-14 by incorporating the Pi54 gene, a widely recognized blast resistance gene, using (MABB) along with selection based on phenotypic traits. NLR-145, a high-yielding rice variety containing the Pi54 gene, was used as the donor parent and crossed with WGL-14 to produce F1 plants. The Pi54MAS marker, which is specific to the Pi54 gene, was employed for foreground selection in F1, BC1F1, BC2F1 and BC3F1 generations, while background selection involved 80 parentals polymorphic SSR markers and phenotypic traits observed in each backcross generation. At BC3F5 generation, 28 lines were selected for their strong resistance to blast, high yields, plant type, grain characteristics and other traits comparable to the recurrent parent. These 28 lines were tested in yield trials during the wet seasons from 2018 to 2020 at the Regional Agricultural Research Station (RARS) in Warangal. At Advanced Varietal Trials (AVT), 3 lines namely WGL-1467, WGL-1472 and WGL-1473 were identified as having strong blast resistance and superior agronomic traits, closely resembling the original WGL-14.

References

  1. Gupta P. A study of trends in rice production in India: Post millennium. International Journal of Science and Research (USR). 2024;13(7):349-53. https://doi.org/10.21275/ SR24705171353
  2. Bhatnagar DVK, Khan DS. Rice matters: From household love to global trade. IIUM Journal of Case Studies in Management. 2024;15(1). https://doi.org/10.31436/ijcsm.v15i1.222
  3. Mondal B, Bisen J, Jambhulkar NN, Tripathi R. Rice supply, demand and exportable surplus in India: Present vis-à-vis thirty years ahead. Oryza. 2022;59(4):504-11. https://doi.org/10.35709/ ory.2022.59.4.13
  4. Khush GS, Jena KK. Current status and future prospects for research on blast resistance in rice (Oryza sativa L.). Conference Paper in Advances in Genetics, Genomics and Control of Rice Blast Disease. 2009;1-10. https://doi.org/10.1007/978-1-4020-9500-9_1
  5. Babujee L, Gnanamanickam SS. Molecular tools for characterization of rice blast pathogen (Magnaporthe grisea) population and molecular marker-assisted breeding for disease resistance. Current Science. 2000;78:248-57.
  6. Ashkani S, Rafii MY, Shabanimofrad M, Miah G, et al. Molecular breeding strategy and challenges towards improvement of blast disease resistance in rice crop. Frontiers in Plant Science. 2015;6:88. https://doi.org/10.3389/fpls.2015.00886
  7. Padmanabhan SY. Studies on forecasting outbreaks of blast disease of rice: I. Influence of meteorological factors on blast incidence at Cuttack. In: Proceedings/Indian Academy of Sciences New Delhi: Springer India; 1965.62(3):117-29. https:// doi.org/10.1007/BF03051084
  8. Sharma TR, Rai AK, Gupta SK, Vijayan J, Devanna BN, Ray S. Rice blast management through host-plant resistance: Retrospect and prospects. Agricultural Research. 2012;1:37-52. https:// doi.org/10.1007/s40003-011-0003-5
  9. Shanmugam A, Suresh R, Ramanathan A, Anandhi P, et al. Characterization of traditional rice varieties for leaf blast-resistant genes Pi5, Pi54, Pi9 and Pi2 using gene-specific markers. Research Biotica. 2023;5(4):158-61. https://doi.org/10.54083/ ResBio/5.4.2023/158-161
  10. Soujanya T, Madamsetty SP, Sandeep G, Hemalatha V, Yamini KN, Bandela E, et al. Improving hybrid rice parental lines for blast resistance by introgression of broad-spectrum resistance genes Pi54 and Pi9 by marker-assisted selection. Plant Breeding. 2023;142(3):300-11. https://doi.org/10.1111/pbr.13093
  11. Zhang M, Wang S, Yuan M. An update on the molecular mechanism of disease resistance genes and their application for the genetic improvement of rice. Molecular Breeding. 2019;39: 154. https://doi.org/10.1007/s11032-019-1056-6
  12. Yin JJ, Zou LJ, Zhu XB, Cao YY, He M, Chen XW. Fighting the enemy: How rice survives the blast pathogens attack. The Crop Journal. 2021;9:543-52. https://doi.org/10.1016/j.cj.2021.03.009
  13. Rai AK, Kumar SP, Gupta SK, Gautam N, Singh NK, Sharma TR. Functional complementation of rice blast resistance gene Pi-k h (Pi54) conferring resistance to diverse strains of Magnaporthe oryzae. Journal of Plant Biochemistry and Biotechnology. 2011;20:55-65. https://doi.org/10.1007/s13562-010-0026-1
  14. Sharma TR, Shanker P, Singh BK, Jana TK, Madhav MS, et al. Molecular mapping of rice blast resistance gene Pi-kh in rice variety Tetep. Journal of Plant Biochemistry and Biotechnology. 2005;14:127-33. https://doi.org/10.1007/BF03263240
  15. Ramkumar G, Srinivasarao K, Mohan KM, Sudarshan I, et al. Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54 (Pikh). Molecular Breeding. 2011;27:129-35. https://doi.org/10.1007/ s11032-010-9538-6
  16. Hittalmani S, Parco A, Mew TV, Zeigler RS, Huang N. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theoretical and Applied Genetics. 2000;100:1121-28. https://doi.org/10.1007/s001220051395
  17. Hari Y, Srinivasarao K, Viraktamath BC, Hari Prasad AS, Laha GS, Ahmed M, et al. Marker-assisted introgression of bacterial blight and blast resistance into IR 58025B, an elite maintainer line of rice. Plant Breeding. 2013;132(6):586-94. https://doi.org/10.1111/ pbr.12056
  18. Balachiranjeevi CH, Bhaskar NS, Abhilash V, Akanksha S, Viraktamath BC, et al. Marker-assisted introgression of bacterial blight and blast resistance into DRR17B, an elite, fine-grain type maintainer line of rice. Molecular Breeding. 2015;35:1-2. https:// doi.org/10.1007/s11032-015-0348-8
  19. Ellur RK, Khanna A, Yadav A, Pathania S, Rajashekara H, Singh VK, et al. Improvement of basmati rice varieties for resistance to blast and bacterial blight diseases using marker-assisted backcross breeding. Plant Science. 2016;242:330-41. https:// doi.org/10.1016/j.plantsci.2015.08.020
  20. Kumar VA, Balachiranjeevi CH, Naik SB, Rambabu R, Rekha G, Harika G, et al. Development of gene pyramid lines of the elite restorer line, RPHR-1005, possessing durable bacterial blight and blast resistance. Frontiers in Plant Science. 2016;7:1195. https://doi.org/10.3389/fpls.2016.01195
  21. Jamaloddin M, Durga Rani CV, Swathi G, Anuradha C, Vanisri S, Rajan CPD, et al. Marker-assisted gene pyramiding (MAGP) for bacterial blight and blast resistance in mega rice variety "Tellahamsa". PLoS One. 2020;15(6). https://doi.org/10.1371/ journal.pone.0234088
  22. Zheng K, Subudhi PK, Domingo J, Maopanty G, Huang N. Rapid DNA isolation for marker-assisted selection in rice breeding. Rice Genet Newsl. 1995;12:255-58. https://doi.org/10.1007/ s40003-014-0095-9
  23. IRRI. Standard evaluation system for rice, IRRI. INGER, 4th Edition, Pd-5. 1996.
  24. Wei T, Simko V, Levy M. Package 'corrplot'. Statistician. 2017;56 (316):24.
  25. Madhu B, Sivakumar S, Manickam S, Murugan M, et al. Improvising cotton (Gossypium hirsutum L.) genotypes for compact plant architecture traits suitable for mechanical harvesting. Indian Journal of Genetics and Plant Breeding. 2023;83(03):398-96. https://doi.org/10.31742/ISGPB.83.3.12
  26. Banoth M, Nunavath US, Bhimireddy S, Konne D, et al. Conventional and molecular breeding strategies for improvement of drought tolerance cultivars in rice: Recent approaches and outlooks. Environment Conservation Journal. 2023;24(4):367-81. https://doi.org/10.36953/ECJ.18202550
  27. Khush GS. What it will take to feed 5.0 billion rice consumers by 2030. Plant Molecular Biology. 2005;59(1):1-6. https:// doi.org/10.1007/s11103-005-2159-5
  28. Ramalingam J, Raveendra C, Savitha P, Vidya V, Chaithra TL, et al. Gene pyramiding for achieving enhanced resistance to bacterial blight, blast and sheath blight diseases in rice. Frontiers in Plant Science. 2020;11. https://doi.org/10.3389/ fpls.2020.591457
  29. Singh R, Sunder S, Dodan DS. Estimation of losses in grain yield due to bacterial blight and neck blast of rice in Haryana. Indian Phytopath. 2013;66(3):249-51.
  30. Arunakanthi B, Srinivasprasad M, Madhanmohan K, et al. Introgression of major blast resistance genes Pi-1, Pi-2 and Pi-kh in indica rice cultivars Samba Mahsuri and Swarna. Journal of Mycology and Plant Pathology. 2008;38:625-30.
  31. Thakur S, Singh PK, Das A, Rathour R, Variar M, Prashanthi SK, et al. Extensive sequence variation in rice blast resistance gene Pi54 makes it broad spectrum in nature. Frontiers in Plant Science. 2015;6:345. https://doi.org/10.3389/fpls.2015.00345
  32. Narayanan NN, Baisakh N, Vera Cruz CM, Gnanamanickam SS, et al. Molecular breeding for the development of blast and bacterial blight resistance in rice cv. IR50. Crop Science. 2002;42 (6):2072-79. https://doi.org/10.2135/cropsci2002.2072
  33. Hospital F, Charcosset A. Marker-assisted introgression of quantitative trait loci. Genetics. 1997;147(3):1469-85. https:// doi.org/10.1093/genetics/147.3.1469
  34. Tanweer FA, Rafii MY, Sijam K, Rahim HA, Ahmed F, Ashkani S, et al. Introgression of blast resistance genes (putative Pi-b and Pi-kh) into elite rice cultivar MR219 through marker-assisted selection. Frontiers in Plant Science. 2015;6:1002. https:// doi.org/10.3389/fpls.2015.01002
  35. Sreewongchai T, Toojinda T, Thanintorn N, Kosawang C, et al. Development of elite indica rice lines with wide spectrum of resistance to Thai blast isolates by pyramiding multiple resistance QTLs. Plant Breeding. 2010;129(2):176-80. https:// doi.org/10.1111/j.1439-0523.2009.01669.x
  36. Gopalakrishnan S, Sharma RK, Anand Rajkumar K, Joseph M, Singh VP, et al. Integrating marker-assisted background analysis with foreground selection for identification of superior bacterial blightresistant recombinants in Basmati rice. Plant Breeding. 2008;127 (2):131-39. https://doi.org/10.1111/j.1439-0523.2007.01458.x
  37. Basavaraj SH, Singh VK, Singh A, Singh A, Singh A, Anand D, et al. Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid. Molecular Breeding. 2010;26:293-305. https:// doi.org/10.1007/s11032-010-9407-3
  38. Hospital F. Size of donor chromosome segments around introgressed loci and reduction of linkage drag in markerassisted backcross programs. Genetics. 2001;158(3):1363-79. https://doi.org/10.1093/genetics/158.3.1363
  39. Aljumaili SJ, Rafii MY, Sakimin SZ, Ahmad K, et al. Backcrossing with marker assistance to introduce broad-spectrum bacterial leaf blight resistance in the Malaysian elite variety MR297. Agriculture. 2023;13(2):372. https://doi.org/10.3390/ agriculture13020372
  40. Singh AK, Ponnuswamy R, Srinivas Prasad M, Sundaram RM, Hari Prasad AS, et al. Improving blast resistance of maintainer line DRR 9B by transferring broad-spectrum resistance gene Pi2 by marker-assisted selection in rice. Physiology and Molecular Biology of Plants. 2023;29(2):253-62. https://doi.org/10.1007/ s12298-023-01291-y
  41. Harshraj Salunkhe AK, Harmeet Singh Janeja BK, Talekar N, et al. Genetic variability, correlation and path-coefficient analysis for yield and yield attributing traits in aerobic rice (Oryza sativa L.). Electronic Journal of Plant Breeding. 2024;15(1):226-32. https://doi.org/10.37992/2024.1501.026

Downloads

Download data is not yet available.