Exploitation of IAA Producing PGPR on mustard (Brassica nigra L.) seedling growth under cadmium stress condition in comparison with exogenous IAA application
DOI:
https://doi.org/10.14719/pst.2019.6.1.440Keywords:
Cadmium stress, IAA, plant growth promoting rhizobacteria, plant growthAbstract
Soil pollution by cadmium (Cd) is a global threat for plants and animals. Exogenous auxin application reduces the Cd stress on plants. Moreover, IAA production by rhizospheric bacteria can play a key role in plant growth and development by promoting cell division, cell elongation, cell differentiation, flowering and lateral root formation. The present study was to evaluate the efficiency of IAA producing, Cd tolerant plant growth promoting rhizobacteria for plant (Brassica nigra L.) growth under Cd stressed condition comparing with the external synthetic auxin application. Lysinibacillus varians and Pseudomonas putida were isolated previously as IAA producing PGPR and selected in this study for their exploitation in plant growth development under Cd stressed condition. The impact of external synthetic IAA application significantly increased the plant growth under Cd amended soil. Whereas, PGPR inoculation also showed significant (p<0.05) elevation in germination percentage, root and shoot length, chlorophyll content and other growth parameters of Cd stressed Brassica plants which were comparative to synthetic IAA application. So, these selected PGPRs (L. varians and P. putida) can be used as biofertilizer which ameliorate the adverse effect of cadmium.
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References
1. Paroda RS, Malik SS. Rice genetic resources, its conservation and use in India. Oryza 1990;27(4):361-9.
2. Swaminathan MS. Enlarging the basis of food security: the role of underutilized species. In: International workshop held at the MS Swaminathan Research Foundation 1999 Feb 19 (pp. 17-19).
3. Zhang YF, He LY, Chen ZJ, Zhang WH, Wang QY, Qian M, Sheng XF. Characterization of lead-resistant and ACC deaminase-producing endophytic bacteria and their potential in promoting lead accumulation of rape. Journal of hazardous materials 2011 Feb 28;186 (2-3):1720-5. https://doi.org/10.1016/j.jhazmat.2010.12.069
4. Kabata-Pendias A, Pendias H. Trace elements in soilsand plants, 3rd edn CRC Press. Boca Raton, FL, USA. 2001.
5. Singh UP, Sarma BK, Singh DP. Effect of plant growth-promoting rhizobacteria and culture filtrate of Sclerotium rolfsii on phenolic and salicylic acid contents in chickpea (Cicer arietinum). Current Microbiology 2003;46 (2):0131-40.
6. Krantev A, Yordanova R, Janda T, Szalai G, Popova L. Treatment with salicylic acid decreases the effect of cadmium on photosynthesis in maize plants. Journal of plant physiology 2008;165(9):920-31. https://doi.org/10.1016/j.jplph.2006.11.014
7. Popova LP, Maslenkova LT, Yordanova RY, Ivanova AP, Krantev AP, Szalai G, Janda T. Exogenous treatment with salicylic acid attenuates cadmium toxicity in pea seedlings. Plant Physiology and Biochemistry 2009;47(3):224-31. https://doi.org/10.1016/j.plaphy.2008.11.007
8. Pal AK, Sengupta C. Effect of plant growth promoting rhizobacteria on early growth of Rice plant (Oryza sativa L.) under Cadmium (Cd) and Lead (Pb) stress condition. International Journal of BioSciences & Technology 2016;9(12).
9. Pal AK, Chakraborty A, Sengupta C. Differential effects of plant growth promoting rhizobacteria on chilli (Capsicum annuum L.) seedling under cadmium and lead stress. Plant Science Today 2018;5(4):1-9. https://doi.org/10.14719/pst.2018.5.4.419
10. Yuan HM, Huang X. Inhibition of root meristem growth by cadmium involves nitric oxide?mediated repression of auxin accumulation and signalling in Arabidopsis. Plant, cell & environment 2016;39(1):120-35. https://doi.org/10.1111/pce.12597
11. Li K, Kamiya T, Fujiwara T. Differential roles of PIN1 and PIN2 in root meristem maintenance under low-B conditions in Arabidopsis thaliana. Plant and Cell Physiology 2015;56(6):1205-14. https://doi.org/10.1093/pcp/pcv047
12. Yuan HM, Xu HH, Liu WC, Lu YT. Copper regulates primary root elongation through PIN1-mediated auxin redistribution. Plant and Cell Physiology 2013;54(5):766-78. https://doi.org/10.1093/pcp/pct030
13. Zelinová V, Alemayehu A, Bo?ová B, Huttová J, Tamás L. Cadmium-induced reactive oxygen species generation, changes in morphogenic responses and activity of some enzymes in barley root tip are regulated by auxin. Biologia 2015;70(3):356-64. https://doi.org/10.1515/biolog-2015-0035
14. Besson-Bard A, Gravot A, Richaud P, Auroy P, Duc C, Gaymard F, Taconnat L, Renou JP, Pugin A, Wendehenne D. Nitric oxide contributes to cadmium toxicity in Arabidopsis by promoting cadmium accumulation in roots and by up-regulating genes related to iron uptake. Plant Physiology 2009;149(3):1302-15. https://doi.org/10.1104/pp.108.133348
15. Farooq H, Asghar HN, Khan MY, Saleem M, Zahir ZA. Auxin-mediated growth of rice in cadmium-contaminated soil. Turkish Journal of Agriculture and Forestry 2015;39(2):272-6. https://doi.org/10.3906/tar-1405-54
16. Ahmad F, Ahmad I, Khan MS. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiological research 2008;163(2):173-81. https://doi.org/10.1016/j.micres.2006.04.001
17. Gordon SA, Weber RP. Colorimetric estimation of indoleacetic acid. Plant physiology 1951;26(1):192. https://doi.org/10.1104/pp.26.1.192
18. Pikovskaya RI. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya 1948;17:362-70.
19. Dye DW. The inadequacy of the usual determinative tests for the identification of Xanthomonas spp. New Zealand Journal of Science 1962;5(4)
20. Bakker AW, Schippers B. Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp-mediated plant growth-stimulation. Soil Biology and Biochemistry 1987;19(4):451-7. https://doi.org/10.1016/0038-0717(87)90037-X
21. Jensen HL. Nitrogen fixation in leguminous plants. II. Is symbiotic nitrogen fixation influenced by Azotobacter. In: Proc. Linn. Soc. NSW 1942;67:205-212.
22. Schwyn B, Neilands JB. Universal chemical assay for the detection and determination of siderophores. Analytical biochemistry 1987;160(1):47-56. https://doi.org/10.1016/0003-2697(87)90612-9
23. Porra RJ, Thompson WA, Kriedemann PE. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta (BBA). Bioenergetics 1989;975(3):384-94. https://doi.org/10.1016/S0005-2728(89)80347-0
24. Abdul-Baki AA, Anderson JD. Vigor determination in soybean seed by multiple criteria 1. Crop science 1973;13(6):630-3. https://doi.org/10.2135/cropsci1973.0011183X001300060013x
25. Shah SS, Mohammad FI, Shafi M, BAKHT J, ZHOU W. Effects of cadmium and salinity on growth and photosynthesis parameters of Brassica species. Pakistan Journal of Botany 2011;43(1):333-40.
26. Aydinalp C, Marinova S. The effects of heavy metals on seed germination and plant growth on alfalfa plant (Medicago sativa). Bulgarian Journal of Agricultural Science 2009;15(4):347-50.
27. Rajkumar M, Freitas H. Effects of inoculation of plant-growth promoting bacteria on Ni uptake by Indian mustard. Bioresource Technology 2008;99(9):3491-8. https://doi.org/10.1016/j.biortech.2007.07.046
28. Kamran MA, Mufti R, Mubariz N, Syed JH, Bano A, Javed MT, Munis MF, Tan Z, Chaudhary HJ. The potential of the flora from different regions of Pakistan in phytoremediation: a review. Environmental Science and Pollution Research 2014;21(2):801-12. https://doi.org/10.1007/s11356-013-2187-7
29. Treesubsuntorn C, Dhurakit P, Khaksar G, Thiravetyan P. Effect of microorganisms on reducing cadmium uptake and toxicity in rice (Oryza sativa L.). Environmental Science and Pollution Research 2018;25(26):25690-701. https://doi.org/10.1007/s11356-017-9058-6
30. Belimov AA, Dietz KJ. Effect of associative bacteria on element composition of barley seedlings grown in solution culture at toxic cadmium concentrations. Microbiological research 2000;155(2):113-21. https://doi.org/10.1016/S0944-5013(00)80046-4
31. Pramanik K, Mitra S, Sarkar A, Soren T, Maiti TK. Characterization of cadmium-resistant Klebsiella pneumoniae MCC 3091 promoted rice seedling growth by alleviating phytotoxicity of cadmium. Environmental Science and Pollution Research 2017;24(31):24419-37. https://doi.org/10.1007/s11356-017-0033-z
32. Pishchik VN, Vorobyev NI, Chernyaeva II, Timofeeva SV, Kozhemyakov AP, Alexeev YV, Lukin SM. Experimental and mathematical simulation of plant growth promoting rhizobacteria and plant interaction under cadmium stress. Plant and Soil 2002;243(2):173-86. https://doi.org/10.1023/A:1019941525758
33. Abbass Z, Okon Y. Plant growth promotion by Azotobacter paspali in the rhizosphere. Soil Biology and Biochemistry 1993;25(8):1075-83. https://doi.org/10.1016/0038-0717(93)90156-6
34. Öncel I, Kele? Y, Üstün AS. Interactive effects of temperature and heavy metal stress on the growth and some biochemical compounds in wheat seedlings. Environmental pollution. 2000;107(3):315-20. https://doi.org/10.1016/S0269-7491(99)00177-3
35. Somashekaraiah BV, Padmaja K, Prasad AR. Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorphyll degradation. Physiologia Plantarum 1992;85(1):85-9. https://doi.org/10.1034/j.1399-3054.1992.850113.x
36. Pätsikkä E, Kairavuo M, Šeršen F, Aro EM, Tyystjärvi E. Excess copper predisposes photosystem II to photoinhibition in vivo by outcompeting iron and causing decrease in leaf chlorophyll. Plant physiology 2002;129 (3):1359-67. https://doi.org/10.1104/pp.004788
2. Swaminathan MS. Enlarging the basis of food security: the role of underutilized species. In: International workshop held at the MS Swaminathan Research Foundation 1999 Feb 19 (pp. 17-19).
3. Zhang YF, He LY, Chen ZJ, Zhang WH, Wang QY, Qian M, Sheng XF. Characterization of lead-resistant and ACC deaminase-producing endophytic bacteria and their potential in promoting lead accumulation of rape. Journal of hazardous materials 2011 Feb 28;186 (2-3):1720-5. https://doi.org/10.1016/j.jhazmat.2010.12.069
4. Kabata-Pendias A, Pendias H. Trace elements in soilsand plants, 3rd edn CRC Press. Boca Raton, FL, USA. 2001.
5. Singh UP, Sarma BK, Singh DP. Effect of plant growth-promoting rhizobacteria and culture filtrate of Sclerotium rolfsii on phenolic and salicylic acid contents in chickpea (Cicer arietinum). Current Microbiology 2003;46 (2):0131-40.
6. Krantev A, Yordanova R, Janda T, Szalai G, Popova L. Treatment with salicylic acid decreases the effect of cadmium on photosynthesis in maize plants. Journal of plant physiology 2008;165(9):920-31. https://doi.org/10.1016/j.jplph.2006.11.014
7. Popova LP, Maslenkova LT, Yordanova RY, Ivanova AP, Krantev AP, Szalai G, Janda T. Exogenous treatment with salicylic acid attenuates cadmium toxicity in pea seedlings. Plant Physiology and Biochemistry 2009;47(3):224-31. https://doi.org/10.1016/j.plaphy.2008.11.007
8. Pal AK, Sengupta C. Effect of plant growth promoting rhizobacteria on early growth of Rice plant (Oryza sativa L.) under Cadmium (Cd) and Lead (Pb) stress condition. International Journal of BioSciences & Technology 2016;9(12).
9. Pal AK, Chakraborty A, Sengupta C. Differential effects of plant growth promoting rhizobacteria on chilli (Capsicum annuum L.) seedling under cadmium and lead stress. Plant Science Today 2018;5(4):1-9. https://doi.org/10.14719/pst.2018.5.4.419
10. Yuan HM, Huang X. Inhibition of root meristem growth by cadmium involves nitric oxide?mediated repression of auxin accumulation and signalling in Arabidopsis. Plant, cell & environment 2016;39(1):120-35. https://doi.org/10.1111/pce.12597
11. Li K, Kamiya T, Fujiwara T. Differential roles of PIN1 and PIN2 in root meristem maintenance under low-B conditions in Arabidopsis thaliana. Plant and Cell Physiology 2015;56(6):1205-14. https://doi.org/10.1093/pcp/pcv047
12. Yuan HM, Xu HH, Liu WC, Lu YT. Copper regulates primary root elongation through PIN1-mediated auxin redistribution. Plant and Cell Physiology 2013;54(5):766-78. https://doi.org/10.1093/pcp/pct030
13. Zelinová V, Alemayehu A, Bo?ová B, Huttová J, Tamás L. Cadmium-induced reactive oxygen species generation, changes in morphogenic responses and activity of some enzymes in barley root tip are regulated by auxin. Biologia 2015;70(3):356-64. https://doi.org/10.1515/biolog-2015-0035
14. Besson-Bard A, Gravot A, Richaud P, Auroy P, Duc C, Gaymard F, Taconnat L, Renou JP, Pugin A, Wendehenne D. Nitric oxide contributes to cadmium toxicity in Arabidopsis by promoting cadmium accumulation in roots and by up-regulating genes related to iron uptake. Plant Physiology 2009;149(3):1302-15. https://doi.org/10.1104/pp.108.133348
15. Farooq H, Asghar HN, Khan MY, Saleem M, Zahir ZA. Auxin-mediated growth of rice in cadmium-contaminated soil. Turkish Journal of Agriculture and Forestry 2015;39(2):272-6. https://doi.org/10.3906/tar-1405-54
16. Ahmad F, Ahmad I, Khan MS. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiological research 2008;163(2):173-81. https://doi.org/10.1016/j.micres.2006.04.001
17. Gordon SA, Weber RP. Colorimetric estimation of indoleacetic acid. Plant physiology 1951;26(1):192. https://doi.org/10.1104/pp.26.1.192
18. Pikovskaya RI. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya 1948;17:362-70.
19. Dye DW. The inadequacy of the usual determinative tests for the identification of Xanthomonas spp. New Zealand Journal of Science 1962;5(4)
20. Bakker AW, Schippers B. Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp-mediated plant growth-stimulation. Soil Biology and Biochemistry 1987;19(4):451-7. https://doi.org/10.1016/0038-0717(87)90037-X
21. Jensen HL. Nitrogen fixation in leguminous plants. II. Is symbiotic nitrogen fixation influenced by Azotobacter. In: Proc. Linn. Soc. NSW 1942;67:205-212.
22. Schwyn B, Neilands JB. Universal chemical assay for the detection and determination of siderophores. Analytical biochemistry 1987;160(1):47-56. https://doi.org/10.1016/0003-2697(87)90612-9
23. Porra RJ, Thompson WA, Kriedemann PE. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta (BBA). Bioenergetics 1989;975(3):384-94. https://doi.org/10.1016/S0005-2728(89)80347-0
24. Abdul-Baki AA, Anderson JD. Vigor determination in soybean seed by multiple criteria 1. Crop science 1973;13(6):630-3. https://doi.org/10.2135/cropsci1973.0011183X001300060013x
25. Shah SS, Mohammad FI, Shafi M, BAKHT J, ZHOU W. Effects of cadmium and salinity on growth and photosynthesis parameters of Brassica species. Pakistan Journal of Botany 2011;43(1):333-40.
26. Aydinalp C, Marinova S. The effects of heavy metals on seed germination and plant growth on alfalfa plant (Medicago sativa). Bulgarian Journal of Agricultural Science 2009;15(4):347-50.
27. Rajkumar M, Freitas H. Effects of inoculation of plant-growth promoting bacteria on Ni uptake by Indian mustard. Bioresource Technology 2008;99(9):3491-8. https://doi.org/10.1016/j.biortech.2007.07.046
28. Kamran MA, Mufti R, Mubariz N, Syed JH, Bano A, Javed MT, Munis MF, Tan Z, Chaudhary HJ. The potential of the flora from different regions of Pakistan in phytoremediation: a review. Environmental Science and Pollution Research 2014;21(2):801-12. https://doi.org/10.1007/s11356-013-2187-7
29. Treesubsuntorn C, Dhurakit P, Khaksar G, Thiravetyan P. Effect of microorganisms on reducing cadmium uptake and toxicity in rice (Oryza sativa L.). Environmental Science and Pollution Research 2018;25(26):25690-701. https://doi.org/10.1007/s11356-017-9058-6
30. Belimov AA, Dietz KJ. Effect of associative bacteria on element composition of barley seedlings grown in solution culture at toxic cadmium concentrations. Microbiological research 2000;155(2):113-21. https://doi.org/10.1016/S0944-5013(00)80046-4
31. Pramanik K, Mitra S, Sarkar A, Soren T, Maiti TK. Characterization of cadmium-resistant Klebsiella pneumoniae MCC 3091 promoted rice seedling growth by alleviating phytotoxicity of cadmium. Environmental Science and Pollution Research 2017;24(31):24419-37. https://doi.org/10.1007/s11356-017-0033-z
32. Pishchik VN, Vorobyev NI, Chernyaeva II, Timofeeva SV, Kozhemyakov AP, Alexeev YV, Lukin SM. Experimental and mathematical simulation of plant growth promoting rhizobacteria and plant interaction under cadmium stress. Plant and Soil 2002;243(2):173-86. https://doi.org/10.1023/A:1019941525758
33. Abbass Z, Okon Y. Plant growth promotion by Azotobacter paspali in the rhizosphere. Soil Biology and Biochemistry 1993;25(8):1075-83. https://doi.org/10.1016/0038-0717(93)90156-6
34. Öncel I, Kele? Y, Üstün AS. Interactive effects of temperature and heavy metal stress on the growth and some biochemical compounds in wheat seedlings. Environmental pollution. 2000;107(3):315-20. https://doi.org/10.1016/S0269-7491(99)00177-3
35. Somashekaraiah BV, Padmaja K, Prasad AR. Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorphyll degradation. Physiologia Plantarum 1992;85(1):85-9. https://doi.org/10.1034/j.1399-3054.1992.850113.x
36. Pätsikkä E, Kairavuo M, Šeršen F, Aro EM, Tyystjärvi E. Excess copper predisposes photosystem II to photoinhibition in vivo by outcompeting iron and causing decrease in leaf chlorophyll. Plant physiology 2002;129 (3):1359-67. https://doi.org/10.1104/pp.004788
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12-01-2019
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Pal AK, Mandal S, Sengupta C. Exploitation of IAA Producing PGPR on mustard (Brassica nigra L.) seedling growth under cadmium stress condition in comparison with exogenous IAA application. Plant Sci. Today [Internet]. 2019 Jan. 12 [cited 2024 Apr. 29];6(1):22-30. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/440
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