Management of Rhizoctonia blight of groundnut using antagonistic effects of bioagents and organic amendments

Pranaballava S; Sweta Shikta Mahapatra; Debashis Dash; Gayatri Biswal; Mihira Kumar Mishra; Shyama Sundar Mahapatra; Abhiram Dash; Jayanta Kumar Mahalik; Kartik Chandra Pradhan

doi:10.14719/pst.4472

Authors

Pranaballava S Dept. of Plant Pathology, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha - 751 003, India https://orcid.org/0009-0002-6038-3712
Sweta Shikta Mahapatra Dept. of Agriculture and Allied sciences, C V Raman Global University, Bhubaneswar, Odisha - 752 054, India https://orcid.org/0000-0002-2401-9887
Debashis Dash Dept. of Agriculture and Allied sciences, C V Raman Global University, Bhubaneswar, Odisha - 752 054, India https://orcid.org/0000-0003-3605-0002
Gayatri Biswal Dept. of Plant Pathology, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha - 751 003, India https://orcid.org/0000-0002-3844-0867
Mihira Kumar Mishra Dept. of Plant Pathology, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha - 751 003, India https://orcid.org/0000-0001-9245-0241
Shyama Sundar Mahapatra Dept. of Plant Pathology, Institute of Agricultural Sciences, Siksha 'O' Anusandhan (Deemed to be University), Bhubaneswar, Odisha -751 030, India https://orcid.org/0009-0004-6719-8020
Abhiram Dash Dept. of Agricultural Statistics, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha - 751 003, India
Jayanta Kumar Mahalik AICRP on Nematodes, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha - 751 003, India https://orcid.org/0000-0001-5246-2336
Kartik Chandra Pradhan AICRP on Groundnut, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha - 751 003, India https://orcid.org/0009-0001-2449-8240

DOI:

https://doi.org/10.14719/pst.4472

Keywords:

Rhizoctonia solani, Trichoderma asperellum, neem seed cake, chlorophyll, phenol

Abstract

Rhizoctonia blight disease caused by Rhizoctonia solani in groundnut crop is one of the most devastating diseases occurring worldwide. The disease affects the morphological and physiological parameters of the crop leading to reduction in pod yield as well as oil yield. The pathogen was isolated locally and identified as Rhizoctonia solani based on molecular characterization. The efficacy of different bioagents in reducing the radial growth of pathogen was tested in vitro and highest mycelia growth inhibition was recorded by Trichoderma asperellum (89.07 %). Among the fungicides tested, 100 % mycelial growth inhibition was observed by use of Carbendazim 50 WP, Tebuconazole 25.9 % EC, Hexaconazole 5 % suspension concentrate (SC) and Tebuconazole 50 % + Trifloxystrobin 25 % water-dispersible granule (WG). Among different organic substances tested in vitro, neem seed cake achieved maximum mycelial growth inhibition of 50.74 % and 54.08 % at 10 % and 20 % concentrations respectively. In the field experiment, treatment with application of neem seed cake to the soil at 500 kg/ha + application of mustard seed cake to the soil at 500 kg/ha + treatment of seeds with Tebuconazole at 1.5 g/kg of seed + treatment of seeds with T. asperellum at 10 g/kg of seed was found to be the best in enhancing plant health, growth promotion and oil yield. The combined treatment of bioagent, fungicide and organic amendment recorded maximum number of branches (14.00), number of leaves (668.33), plant dry weight (64.17 g), 100 pod weight (65.00 g) and oil yield (47.33 %) compared to the control and other treatments along with reduction of the disease (59.61 %). In the physiological parameters study, the same treatment also recorded maximum pigment contents viz. Chlorophyll a (1.843 mg/g), Chlorophyll b (0.555 mg/g), total chlorophyll (2.397 mg/g) and carotenoid content (0.084 mg/g) but with minimum phenol content (1.693 mg/g). Thus, it can be concluded that integration of selective inputs in the combined treatment of Neem seed cake, Mustard seed cake, Tebuconazole and T. asperellum could enhance the plant health, morphological growth and physiological parameters and increased the oil yield in groundnut along with reduction of the disease.

Downloads

Download data is not yet available.

References

Kumar PV. WMO/CAgM Guide to Agricultural Meteorological Practices (GAMP),

Agrometeorology and Groundnut Production. 2007;13.

Dubey SC. Biological management of web blight of groundnut (Rhizoctonia solani). Journal of Mycology and Plant Pathology. 2000;30(1):89-90.

Umbon C, Ao NT, Banik S, Neog P, Chakruno P. Effect of chemicals on yield losses in soybean due to Rhizoctonia aerial blight. Journal of Mycopathological Research. 2024;62(2):401-07.

Thiessen L. Improving upon the management of soilborne diseases of peanut inwest texas. M.Sc (Agri). Texas Tech University, Lubbock, USA. 2012.

Thiessen LD and Woodward JE. Diseases of peanut caused by soilborne pathogens in the Southwestern United States. International scholarly research notices. 2012;517905. https://doi.org/10.5402/2012/517905

Rawal P, Sharma P, Dodiya NS, Joshi A. Evaluation of fungicides, neem bioformulations and biocontrol agent for the management of root rot of Safed Musli caused by Rhizoctonia solani. Journal of Mycology and Plant Pathology. 2013;43(3):297-305.

Jadon KS, Thirumalaisamy PP, Kumar V, Koradia VG, Padavi RD. Management of soil borne diseases of groundnut through seed dressing fungicides. Crop Protection. 2015;78:198-203. https://doi.org/10.1016/j.cropro.2015.08.021

Choudhary A, Ashraf S. Utilizing the combined antifungal potential of Trichoderma spp. and organic amendments against dry root rot of mungbean. Egyptian Journal of Biological Pest Control. 2019;29:83. https://doi.org/10.1186/s41938-019-0187-8

Kühn JG. Die Krankheiten der Kulturgewächse: ihreUrsachen und ihreVerhütung. G. Vosselmann. 1858.

Rangaswami G, Mahadevan A. (Eds). Disease of crop plants in India, Prentice-Hall of India Private Limited Publisher, New Delhi, India. 2004;507 p.

Dennis C, Webster J. Antagonistic properties of species-groups of Trichoderma: II. Production of volatile antibioticsm. Transactions of the British Mycological Society. 1971;57:363-69. https://doi.org/10.1016/S0007-1536(71)80050-5

Arora DK, Upadhyay RK. Effect of fungal staling growth substances on colony interaction. Plant and Soil. 1978;49:685-90. https://doi.org/10.1007/BF02183297

Borum DF, Sinclair JB. Evidence of systemic fungicides protection against R. solani with vitavax in cotton seedlings. Phytopathology. 1968; 58:976-80.

Nene VL, Thapliyal PN. Fungicides in plant disease control, Oxford & IBH Publ. Co. Pvt. Limited, New Delhi, India. 1993;507.

Ramamurthi J, Raju SM, Shubhada PB. Analysis of oil content of groundnuts by nuclear magnetic resonance spectrometry. Journal of the Science of Food and Agriculture. 1985;36:162-66. https://doi.org/10.1002/jsfa.2740360306.

Aron D. Copper enzymes isolated chloroplasts, polyphenoloxidase in Beta vulgaris. Plant Physiology. 1949;24:1-15. https://doi.org/10.1104/pp.24.1.1

Lichtenthaler LK, Wellburn AR. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans. 1983;11(5):591-92. https://doi.org/10.1042/bst0110591

Bray HG, Thorpe WV. Analysis of phenolic compounds of interest in metabolism. J Meth Biochem Anal. 1954;1:27-52. https://doi.org/10.1002/9780470110171.ch2

Gomez KA, Gomez AA. Statistical procedures for agricultural research, 2nd edition, John wiley Sons, Singapore. 1984;683.

Trillas MI, Casanova E, Cotxarrera L, Ordovas J, Borrero C, Aviles M. Composts from agricultural waste and the Trichoderma asperellum strain T-34 suppress Rhizoctonia solani in cucumber seedlings. Biological Control. 2006;39(1):32-38. https://doi.org/10.1016/j.biocontrol.2006.05.007

Almaghasla MI, El-Ganainy SM, Ismail AM. Biological activity of four Trichoderma species confers protection against Rhizoctonia solani, the causal agent of cucumber damping-off and root rot diseases. Sustainability. 2023;15:7250. https://doi.org/10.3390/su15097250

Lal R, Basayya AA, Sobita S. Management of seedling rot of chilli (Capsicum annuum L.) using Trichoderma spp. and Pseudomonas fluorescens. Annals of Plant Protection Sciences. 2013;21(2):387-90.

Barakat RM, Al-Mahareeq F, Ali-Shtayeh MS, AL-Masri MI. Biological control of Rhizoctonia solani by indigenous Trichoderma spp. isolates from Palestine. Hebron University Research Journal. 2007;1(3):1-15.

Usendi PN, Giri GK, Kabade SH. Evaluation of fungicides, bioagents and botanicals against Rhizoctonia spp. incitant of sheath blight of rice. International Journal of Current Microbiology and Applied Sciences. 2020;9(08):3039-46. https://doi.org/10.20546/ijcmas.2020.908.343

Tetarwal JP. Integrated disease management root rot of soybean. M.Sc. (Ag.) Thesis, MPUAT, Udaipur (Raj); 2011.

Bag MK. Efficacy of a new fungicide 'Trifloxystrobin 25 % + Tebuconazole 50 %' 75WG against sheath blight (Rhizoctonia solani Kuhn.) of rice. Journal of Crop and Weed. 2009;5:224-26.

Surulirajan M, Kandhari J. Screening of Trichoderma viride and fungicides against Rhizoctonia solani. Annals of Plant Protection Sciences. 2003;11(2):283-84.

Meena B, Muthusamy M. Effect of organic soil amendments against rice sheath blight. Indian Phytopathology. 1999;52(1):92-93.

Shivran M, Ghasolia RP, Bajaya T. Evaluation of bio-control agents and organic amendments for managing root rot (Rhizoctonia solani) of cluster bean (Cyamopsis tetragonoloba). Indian Journal of Agricultural Research. 2023;57(5):691-96.

Lenka S, Kun KB. In vitro effect of organic amendment through oil cakes on Rhizoctonia solani Kuhn causing sheath blight disease in Rice. Journal of Plant Protection and Environment. 2014;11(2):88-90.

Kabdwal BC, Sharma R, Kumar A, Kumar S, Singh KP, Srivastava RM. Efficacy of different combinations of microbial biocontrol agents against sheath blight of rice caused by Rhizoctonia solani. Egyptian Journal of Biological Pest Control. 2023;33:29. https://doi.org/10.1186/s41938-023-00671-6

Srivastava R, Khalid A, Singh US, Sharma AK. Evaluation of arbuscular mycorrhizal fungus Pseudomonas fluorescens and Trichoderma harzianum formulation against Fusarium oxysporum f.sp. lycopersici for the management of tomato wilt. Biol Cont. 2010;53:24-31. https://doi.org/10.1016/j.biocontrol.2009.11.012

Upmanyu S, Gupta SK, Shyam KR. Innovative approaches for the management of root rot and web blight (Rhizoctonia solani) of French bean. Journal of Mycology and Plant Pathology. 2002;32(3):317-31.

Mithen R. Leaf glucosinolate profiles and their relationship to pest and disease resistance in oilseed rape. Euphytica. 1992;63:71-83. https://doi.org/10.1007/978-94-017-0954-5_6

Kirkegaard JA, Wong PTW, Desmarchelier JM. In vitro suppression of fungal root pathogens of cereals by Brassica tissues. Plant Pathol. 1996;45:593-03. https://doi.org/10.1046/j.1365-3059.1996.d01-143.x

Grichar WJ, Jaks AJ, Woodward J. Using prothioconazole plus tebuconazole

for foliar and soilborne disease control in Texas peanut. Crop Manag. 2010. https://doi.org/10.1094/CM-2010-0405-02-RS.

Akhter W, Bhuiyan MKA, Sultana F, Hossain MM. Integrated effect of microbial antagonist, organic amendment and fungicide in controlling seedling mortality (Rhizoctonia solani) and improving yield in pea (Pisum sativum L.). Comptes Rendus Biologies. 2015;338(1):21-28. https://doi.org/10.1016/j.crvi.2014.10.003

Hossain MM, Hossain N, Sultana F, Islam SMN, Islam MT, Bhuiyan MKA. Integrated management of Fusarium wilt of chickpea (Cicer arietinum L.) caused by Fusarium oxysporum f. sp. ciceris with microbial antagonist, botanical extract and fungicide. Afr J Biotechnol. 2013;12:4699-706. https://doi.org/10.5897/AJB2013.12503

Sultana N, Ghaffar A. Effect of fungicides, microbial antagonists and oilcakes in the control of Fusarium oxysporum, the cause of seed rot and root infection of bottle gourd and cucumber. Pak J Bot. 2013;45:2149-156.

Benhamou N, Gagne S, Quere DL, Dehbi L. Bacterial-mediated induced resistance in cucumber: beneficial effect of the endophytic bacterium Serratia plymuthica on the protection against infection by Pythium ultimum. Phytopathol. 2000;90:45-56. https://doi.org/10.1094/PHYTO.2000.90.1.45

Atwa M. Combination of biocontrol agents for controlling soybean damping-off caused by Rhizoctonia solani. Egyptian Journal of Phytopathology. 2018;46(2):15-38. https://doi.org/10.21608/ejp.2018.91702

Karthikeyan V, Sankaralingam A, Nakkeeran S. Management of groundnut root rot with biocontrol agents and organic amendments. Archives of Phytopathology and Plant Protection. 2006;39(3):215-23. https://doi.org/10.1080/03235400500094225

Sharma P, Sain SK. Use of biotic agents and abiotic compounds against damping off of cauliflower caused by Pythium aphanidermatum. Indian Phytopathology. 2005;58(4):395.

Basco MJ, Bisen K, Keswani C, Singh HB. Biological management of Fusarium wilt of tomato using biofortified vermicompost. Mycosphere. 2017;8(3):467-83. https://doi.org/10.5943/mycosphere/8/3/8

Jamil A, Musheer N, Ashraf S. Antagonistic potential of Trichoderma harzianum and Azadirachta indica against Fusarium oxysporum f. sp. capsici for the management of chilli wilt. Journal of Plant Diseases and Protection. 2021;128:161-72. https://doi.org/10.1007/s41348-020-00383-1

Rizvi R, Singh G, Safiuddin ARA, Tiyagi SA, Mahmood I. Sustainable management of root-knot disease of tomato by neem cake and Glomus fasciculatum. Cogent Food Agric. 2015;1:1008859. https ://doi.org/10.1080/23311 932.2015.10088 59

Rahdari P, Tavakoli S, Hosseini SM. Studying of salinity stress effect on germination, proline, sugar, protein, lipid and chlorophyll content in purslane (Portulaca oleracea L.) leaves. J Stress Physiol Biochem. 2012;8:182-93.