Optimizing in vitro micropropagation strategies in Santalum album L. through different explants

M Gowsalya; S R Jude; K Hemaprabha; R Revathi; M Mathivanan; K T Parthiban; I Sekar; M Kiruba; P P Sujith; V Kabinesh

doi:10.14719/pst.5991

Authors

Gowsalya M Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam 641 301, Tamil Nadu, India https://orcid.org/0009-0002-4224-9042
Jude Sudhagar R Dryland Agricultural Research Station, Tamil Nadu Agricultural University, Chettinad 630 102, Tamil Nadu, India https://orcid.org/0000-0002-3916-3902
Hemaprabha K Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam 641 301, Tamil Nadu, India https://orcid.org/0000-0002-0677-0490
Revathi R Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam 641 301, Tamil Nadu, India https://orcid.org/0000-0002-4875-8748
Mathivanan M Anbil Dharmalingam Agricultural College and Research Institute, Tamil Nadu Agricultural University, Trichy 620 027, Tamil Nadu, India
Parthiban K T Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam 641 301, Tamil Nadu, India https://orcid.org/0000-0001-5135-5846
Sekar I Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam 641 301, Tamil Nadu, India https://orcid.org/0009-0008-1157-3038
Kiruba M Horticulture Research Station, Tamil Nadu Agricultural University, Yercaud 636 601, Tamil Nadu, India https://orcid.org/0000-0002-9329-2705
Sujith P P Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam 641 301, Tamil Nadu, India https://orcid.org/0009-0007-3292-9542
Kabinesh V Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam 641 301, Tamil Nadu, India https://orcid.org/0009-0003-7830-8507

DOI:

https://doi.org/10.14719/pst.5991

Keywords:

Indian sandalwood, explants disinfection, micropropagation, plant growth regulators, shoot induction

Abstract

This study focuses on optimization of in vitro propagation protocols for Santalum album L. (Indian sandalwood) to address the increasing global demand and to aid in conservation aspects. This investigation evaluated various micropropagation parameters including explant types, basal media, disinfection methods and plant growth regulators (PGR). Murashige and Skoog medium consistently outperformed other media, with shoot tips showing the highest morphogenetic response (66.66%). Surface disinfection with 4% NaOCl for 10 min was the most effective and resulted in the explant survival rate of 86%. For shoot induction, 2 mg/L kinetin in MS medium resulted in the highest number of shoots (3.81) and longest shoots (5.39 cm) after 60 days. An effect was observed when PGR was combined with 5.0 mg/L kinetin + 2.0 mg/L BAP, corresponding to a shoot induction rate of 65.75%. Root induction was recorded at 14.79% after 10 days with the best treatment (MS + 1 mg/L IBA). Callus culture showed limited success as only one treatment (MS + 1 mg/L BAP + 25% coconut water) showed a regenerative response of 7.14%. These results provide a foundation for micropropagation of Sandal while highlighting areas that require further optimization, particularly in root induction and acclimation phases.

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