Production of poly hydroxy butyrate (PHB) from Eichhornia crassipes through microbial fermentation process
DOI:
https://doi.org/10.14719/pst.2019.6.sp1.673Keywords:
Bacteria, Bioplastic, Polyhydroxybutyrate, Optimum conditionAbstract
Polyhydroxybutyrate (PHB) is one of the highly biodegradable and biologically acceptable thermoplastics synthesized by many microorganisms collectively called polyhydroxyalkanoates (PHAs). All available biopolymers are viewed as perfect answers for the resolution of natural contamination issue by supplanting ordinary plastic business. They are likewise utilized as osteosclerotic stimulants attributable to their piezoelectric properties, in bone plates, during operations as suture material and vein substitutions. Synthesis of PHB is found in a wide range of Gram’s negative and gram’s positive bacteria belonging to distinct genera. Optimum culture condition for the PHB producing microbes are provided, including restricted centralization of nitrogen, sulfur, phosphorus, or the trace elements and maximum convergence of carbon source Indeed, to market PHAs, significant exertion has been dedicated towards a decline in the production cost through the improvement of bacterial strains and enhancing effectiveness of recovery/fermentation procedure. This is being done considering the fact that substrate prices show the greatest impact on PHA's manufacturing cost. The price of the substrate used has the most significant influence on the production cost of PHA. In this research, a potential bacterial strain was isolated from the soil and tested for its PHB producing ability. The use of cheaper substrate for lowering the cost is prerequisite. For PHB production, water hyacinth was used as a carbon source. Bacterial growth was optimized for maximum PHB production. The optimum condition was found to be 30 °C, 8% substrate concentration and 72 h of incubation time.
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2. Pathak S, Sneha CLR, Mathew BB. Bioplastics: its timeline based scenario & challenges. J Polym Biopolym Physics Chem. 2014;2:84-90. https://doi.org/10.12691/jpbpc-2-4-5
3. Chen YJ. Bioplastics and their role in achieving global sustainability. J Chem Pharm Res. 2014;6:226-31.
4. Nehra K, Jaglan A, Shaheen A, Yadav J, Lathwal P. Production of Poly-?-Hydroxybutyrate (PHB) by bacteria isolated from rhizospheric soils. Int J Microbial Resource Technol 2015; 2: 38-48.
5. Getachew A, Woldesenbet F. Production of biodegradable plastic by polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material. BMC Res Notes. 2016;12:509. https://doi.org/10.1186/s13104-016-2321-y
6. Law JH, Slepecky RA. Assay of poly-?-hydroxybutyric acid. J Bacteriol. 1961;82:33-36.
7. Poirier Y, Nawrath C, Somerville C. Production of polyhydroxyalkanoates, a family of biodegradable plastics and elastomers in bacteria and plants. Biotechnol. 1995;13:142-50. https://doi.org/10.1038/nbt0295-142
8. Kumar S, Mudaliar MSN, Reddy KMK, Chakrabarti J. Production of biodegradable plastics from activated sludge generated from a food processing industrial waste water. Bioresource Technol. 2004;95:327-30. https://doi.org/10.1016/j.biortech.2004.02.019
9. Singh G, Mittal A, Kumari A, Goel V, Aggarwal NK, Yadav A. Optimization of poly-B-hydroxybutyrate production from Bacillus species. Europ J Biol Sc. 2011;3:112-16.
10. Soam A, Singh AK, Singh R, Shahi SK. Optimization of culture conditions for bio-polymer producing Bacillus mycoides (WSS2) bacteria from sewage. Curr Discover. 2012;1:27-32.
11. Gregersen T. (1978). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol. 1978;5:123-27. https://doi.org/10.1007/BF00498806
12. Indira M, Karlapudi AP, Venkateswarulu TC, Babu DJ, Nath SB, Kodali VP. Isolation, screening and extraction of polyhydroxybutyrate (PHB) producing bacteria from sewage sample. Int J PharmTech Res. 2014;6:850-57.
13. Campos MI, Figueiredo TVB, Sousa LS, Druzian JI. The influence of crude glycerin and nitrogen concentrations on the production of PHA by Cupriavidus necator using a response surface methodology and its characterizations. Ind Crop Prod. 2014;52:338-46. https://doi.org/10.1016/j.indcrop.2013.11.008
14. Bengtsson S, Pisco AR, Johansson P, Lemos PC, Reis MAM. Molecular weight and thermal properties of polyhydroxyalkanoates produced from fermented sugar molasses by open mixed cultures. J Biotechnol. 2010;147:172-79. https://doi.org/10.1016/j.jbiotec.2010.03.022
15. Alarfaj AA, Arshad M, Sholkamy EN, Munusamy MA. Extraction and Characterization of Polyhydroxybutyrates (PHB) from Bacillus thuringiensis KSADL127 Isolated from Mangrove Environments of Saudi Arabia. Braz Arch Biol Technol. 2015;58:781-88. http://dx.doi.org/10.1590/S1516-891320150500003
16. Hu S, McDonald AG, Coats ER. Characterization of polyhydroxybutyrate biosynthesized from crude glycerol waste using mixed microbial consortia. J Appl Polym Sci. 2013;129:1314-21. https://doi.org/10.1002/app.38820
17. Devi AB, Nachiyar CV, Kaviyarasi T, Samrot AV. Characterization of polyhydroxybutyrate synthesized by Bacillus cereus. Int J Pharm Pharm Sci. 2015;7:140-44. https://innovareacademics.in/journals/index.php/ijpps/article/view/4378
18. Altaee N, Fahdil A, Yousif E, Sudesh K. Recovery and subsequent characterization of polyhydroxybutyrate from Rhodococcus equi cells grown on crude palm kernel oil. J Taibah Univ Sci. 2016;10:543-50. https://doi.org/10.1016/j.jtusci.2015.09.003
19. Pradhan S, Borah AJ, Poddar MK, Dikshit PK, Rohidas L, Moholkar VS. Microbial production, ultrasound-assisted extraction and characterization of biopolymer polyhydroxybutyrate (PHB) from terrestrial (P. hysterophorus) and aquatic (E. crassipes) invasive weeds. Bioresource Technol. 2017;242:304-10. https://doi.org/10.1016/j.biortech.2017.03.117
20. Peter HY, Chua H, Huang AL, Ho KP. Conversion of industrial food wastes by Alcaligenes latus into polyhydroxyalkanoates. In: Twentieth Symposium on Biotechnology for Fuels and Chemicals. Humana Press, Totowa, NJ. 1999;445-54.
21. Omar S, Rayes A, Eqaab A, Voß I, Steinbüchel A. Optimization of cell growth and poly (3-hydroxybutyrate) accumulation on date syrup by a Bacillus megaterium strain. Biotechnol lett. 2001;23:1119-23. doi: https://doi.org/10.1023/A:1010559800535
22. Chee JY, Tan Y, Samian MR, Sudesh K. Isolation and characterization of a Burkholderia sp. USM (JCM15050) capable of producing polyhydroxyalkanoate (PHA) from triglycerides, fatty acids and glycerols. J Polym Environ. 2010;18:584-92. doi: https://doi.org/10.1007/s10924-010-0204-1
23. Thakor N, Trivedi U, Patel KC. Biosynthesis of medium chain length poly (3-hydroxyalkanoates)(mcl-PHAs) by Comamonas testosteroni during cultivation on vegetable oils. Bioresource Technol. 2005;96:1843-50. doi: 10.1016/j.biortech.2005.01.030
24. Yu J, Stahl H. Microbial utilization and biopolyester synthesis of bagasse hydrolysates. Bioresource Technol. 2008;99:8042-48. https://doi.org/10.1016/j.biortech.2008.03.071
25. Lee WH, Loo CY, Nomura CT, Sudesh K. Biosynthesis of polyhydroxyalkanoate copolymers from mixtures of plant oils and 3-hydroxyvalerate precursors. Bioresource Technol. 2008;99:6844-51. https://doi.org/10.1016/j.biortech.2008.01.051
26. Cavalheiro JMBT, de-Almeida MCMD, Grandfils C, da-Fonseca MMR. Poly (3-hydroxybutyrate) production by Cupriavidus necator using waste glycerol. Proc Biochem. 2009;44:509-15. https://doi.org/10.1016/j.procbio.2009.01.008
27. Bhuwal AK, Singh G, Aggarwal NK, Goyal V, Yadav A. Isolation and screening of polyhydroxyalkanoates producing bacteria from pulp, paper, and cardboard industfigry wastes. Int J Biomat. 2013;1-10. http://dx.doi.org/10.1155/2013/752821
28. Preethi K, Umesh VM. Water hyacinth: a potential substrate for bioplastic (PHA) production using Pseudomonas aeruginosa. Int J Appl Res. 2015;1:349-54
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