Occurrence of thermophilic fungal communities and its growth rate on different media and temperatures from available natural substrates

Authors

  • Ganesh Mallikarjun Birajdar Research Laboratory, Department of Botany, Arts, Science and Commerce College, Naldurg, Osmanabad 413 602, Maharashtra, India
  • Vivekanand Ramchandra Kumbhar Research Laboratory, Department of Botany, Arts, Science and Commerce College, Naldurg, Osmanabad 413 602, Maharashtra, India
  • Kalyan K Kadam Department of Botany, K.K.M. College, Manwat, Parbhani 431 505, Maharashtra, India
  • Udhav Narba Bhale Research Laboratory, Department of Botany, Arts, Science and Commerce College, Naldurg, Osmanabad 413 602, Maharashtra, India

DOI:

https://doi.org/10.14719/pst.2020.7.2.719

Keywords:

Organic substrates, Thermophilic fungi, Culture media

Abstract

Thermophilic fungi are the chief components of the microflora that develops in heaped masses of plant materials, piles of agricultural and forestry products and other accumulation of organic matter. In this investigation, survey was conducted and collected different compost samples from eight localities of Osmanabad district. Among these localities, Naldurg and Dhoki sites showed highest number of organic substrates, while least in Itkal. Physicochemial properties i.e. nitrogen, phosphorus, potassium, total organic matter, total carbon and moisture were analysed from available composts and among them nitrogen was highest in molasses. Isolation of fungi was made from substrates and among five substrates, vermicompost and Farm Yard Manure (FYM) were recorded for highest species richness and % incidence while less in molasses. Among isolated thermophilic fungi, Aspergillus niger, A. flavus, Rhizoctonia solani, A. fumigatus and Rhizopus sp. found abundant in all the substrates and temperature ranges. Fungal species A. niger, Mucor mucedo, A. flavus and Pythium sp. Highest growth on all three media viz. Potato dextrose agar (PDA), Czapek’s Dox Agar (CZA) and Martins Rose Bengal (MBR) were recorded. A. niger and Rhizopus sp. were found dominant in tested media and temperature. Generally, the fungi show very little growth at 65 °C. Every temperature gradient showed the growth incidence in all substrates but room temperature (RT) and 35 °C showed richness of fungal incidence. Even some species showed the growth on some substrates at higher temperate (65 °C) to some extent.

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References

1. Dix NJ, Webster J. Fungal Ecology. Chapman & Hall, London, 1995; p. 549

2. Naik PS. Studies on microbial consortia for production and Enrichment of bio-compost from grapevine residues. Master of Science (Agriculture) Thesis submitted to the Department of Agricultural Microbiology College of Agriculture, Dharwad University of Agricultural Sciences, Dharwad 580 005. 2017

3. Asghar HN, Ishaq M, Zahir ZA, Khalid M, Arshad M. Response of radish to integrated use of nitrogen fertilizer and recycled organic waste. Pak J Bot. 2006;38:691-700. https://pdfs.semanticscholar.org

4. Singleton P, Sambury D. Dictionary of Microorganisms. 4th ed., John Willey and Sons Press, New York; 1998; p.1017

5. Tilman D, Cassman KG, Matson, PA. Naylor R, Polasky S. Agricultural sustainability and intensive production practices. Nature. 2002;418:671-77. https://doi.org/10.1038/nature01014

6. Goyal S, Dhull SK, Kapoor KK. Chemical and biological changes during composting of different organic wastes and assessment of compost maturity. Bioresour Technol. 2005;96,1584-91. https://doi.org/10.1016/j.biortech.2004.12.012

7. Zmora-Nahum S, O Markovitch, J Tarchitzky, Chen Y. Dissolved organic carbon (DOC) as a parameter of compost maturity. Soil Biol Biochem. 2005;37:2109-16. https://doi.org/10.1016/j.soilbio.2005.03.013

8. Hernandez T, G Masciandaro JI, Moreno, Garcia C. Changes in organic matter composition during composting of two digested sewage sludges. Waste Manage. 2006;26:1370-76. https://doi.org/10.1016/j.wasman.2005.10.006

9. Anastasi A, GC, Varese, Marchisio VF. Isolation and identification of fungal communities in compost and vermicompost. Mycologia. 2005;97(1):33-44. https://doi.org/10.1080/15572536.2006.11832836

10. Trautmann N. The Science and Engineering of Composting, Cornell Composting. Cornell University Press, Ithaca, New York, 1992

11. Subbiah BV, Asija GL. A rapid procedure for determination of available nitrogen in soils. Curr Sci. 1956;259-60

12. Sahilemedhin S, Bekele T. Procedures for soil and plant analysis. National soil research center Ethiopian Agricultural Research Organization. Addis Ababa, Ethiopia; 2000

13. Olsen SR, CV Cole, FS Watanabe, Dean LA. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. USDA Circular No. 939. 1954

14. Bray RH, Kurtz LT. Determination of total, organic and available forms of phosphorus in soils. Soil Sci. 1945;59:30-45

15. Milner BA, Whiteside PJ. Introduction to Atomic Absorption Spectrophotometry. 3rd Ed. Pye Unicam Ltd, York Street, Cambridge, England. 1984; p. 52

16. Hanway JJ, Heidel H. Soil analysis methods as used in Iowa State College Soil Testing Laboratory. Iowa Agri. 1952;57:1-31

17. Apinis AE. Thermophilous fungi of coastal grasslands in soil organisms. Proceedings of the colloquium on soil fanna, soil microflora and their relationships by J Doeksen, J Van der Drift (eds). North Holland, Amsterdam. 1963; pp. 427-38

18. Waksman SA, Umbreit WW, Cordon TC. Thermophilic actinomycetes and fungi in soils and in composts. Soil Sci. 1939;47:37-62

19. Girisham S. Studies on mycotoxin producing fungi associated with pearl millet (Pennisetum americanum L.). Ph.D thesis. Kakatiya University, Warangal. 1986

20. Subramanian CV. Hypomycetes:an account of Indian species except cercosporae. Indian Council of Agricultural Research, New Delhi. 1971; p. 463

21. Barnett HL, Hunter BB. Illustrated Genera of Imperfecti fungi. Burgess Publishing Company, Minneeapolis, Minnesota. 1972; p. 95

22. Mukadam DS. The Illustrated kingdom of fungi. Akshar Ganga Prakashan, Aurangabad. 1997; pp. 66-91

23. Kumar RR, Sreelatha B, Girisham S, Reddy SM. Incidence of thermophilic fungi from different substrates in Andhra Pradesh (India). International Journal of Pharma and Bio sciences. 2010;1(3):BS33. http://www.ijpbs.net/issue-3/34.pdf

24. Salar RK, Aneja KR. Thermophilic Fungi: Taxonomy and Biogeography. Journal of Agricultural Technology. 2007; 3(1):77-107

25. Sikora LJ, Szmidt RAK. Nitrogen sources, mineralization rates, and nitrogen nutrition benefits to plants from composts. In: Compost utilization in horticultural cropping systems. Ed. PJ Stoffella, BA Kahn. Boca Raton: Lewis Publishers. 2001; pp. 287–320

26. Malinger FB Gotze, P Dreher, J Geszti, Weisstein C. Nitrogen in biowaste and yard waste compost: Dynamics of mobilisation and availability - A review. European Journal of Soil Biology, 2003;39:107–16

27. Yadav A, R Gupta, VK Garg. Organic manure production from cow dung and biogas plant slurry by vermicomposting under field conditions. International Journal of Recycling of Organic Waste in Agriculture. 2013;2,21. https://doi.org/10.1186/2251-7715-2-21

28. Benito M, A Massaguer, A Molinera, De Antonio R, De Antonio. Use of pruning waste compost as a component in soil less growing media. Bioresour. Technol. 2006; 97:2071–76

29. Rosen CJ, TR Halbach, Swanson BT. Horticultural uses of municipal solid waste components. Hortic. Technol. 1993;3:167–73

30. Batjes NH. Total carbon and nitrogen in the soils of the world. Eur. J. Soil Sci. 1996;47:151–63. https://doi.org/10.1111/ejss.12115

31. Blom BD, Emerson R. Studies on thermophily in fungi with particular reference to a new thermophilic Penicillium. Amer J Bot. 1962;49:665

32. Cooney DG. The thermophilic and thermotolerentmolds and Actinomycetes of mushroom compost during peak heating. Mycologia. 1964;56:267-84

33. Crisan EV, Fergus CL. Isolation and culture of thermophilic fungi. Boyce Thompson Inst. 1964;22:291

34. Cooney DG, Emerson R. Thermophilic fungi San. Francisco, W H Freeman, 1964; p.27

35. Ramesh CH, Anil K. Studies on thermophilic fungi from different substrates of Dharwad, Karnataka. In: Proceeding of the National conference on Mycology plant pathology and Microbial Biotechnology, Department of Botany, Osmania University (Bagynarayana et al. Eds). Hyderabad. BS Publiations, Hyderabad. 2005; pp.96-103

36. Maheshwari R, Kamalam PT, Balasubrmanyam DV. The biogeography of thermophilic fungi. Curr Sci. 1987; 56:151-55

37. Cowan, MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews, 1999;12 (4):564-82

38. Ashraf R, Faiza S, Tasneem AA. Association of fungi, bacteria and actinomycetes with different composts. Pak J Bot. 2007;39(6):2141-51

39. Kurlekar SL. Effect of variable temperature on the growth of thermophilic fungi. Ad Plant Sci. 2014; 27(II):503-04

40. Bhale UN, VS Sawant, PP Sarwade, Rajkonda JN. Thermophilic fungi from different substrates of Osmanabad District. Bioinfolet. 2008;5(3):248-51

41. Wouters IM, S Spaan, J Douwes, G Doekes, Heederik D. Overview of personal occupational exposure levels to inhale dust, endotoxin, ? (1-3)-glucan and fungal extracellular polysaccharides in the waste management chain. Ann Occup Hyg. 2005;47:1-15. https://doi.org/10.1093/annhyg/mei047

42. Iranzo M, JV Canizares, L Roca-Perez, I Sainz-Pardo, S Mormeneo, Boluda R. Characteristic of rice straw and sewage sludge as composting materials in Valencia (Spain). Bioresource Technology. 2004;95(1):107-12. https://doi.org/10.1016/j.foodchem.2015.04.083

43. Pathak AK, MM Singh, V Kumar, S Arya, Trivedi AK. Assessment of physico chemical properties and microbial community during composting of municipal solid waste (viz. KItchen waste) at Jhansi City, U. P. (India). Recent Research in Science and Technology. 2012;4(4):10-14

Published

01-04-2020

How to Cite

1.
Birajdar GM, Kumbhar VR, Kadam KK, Bhale UN. Occurrence of thermophilic fungal communities and its growth rate on different media and temperatures from available natural substrates. Plant Sci. Today [Internet]. 2020 Apr. 1 [cited 2024 Nov. 4];7(2):172-7. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/719

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Research Articles