Evaluation of effective protein extraction procedure to profile petiole of Moringa oleifera

Authors

  • Zetty Amirah Zulkifli Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor-Bahru, Malaysia
  • Zaidah Rahmat 1) Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor-Bahru, Malaysia; 2) Chemical Management Unit, University Laboratory Management Centre (PPMU),Universiti Teknologi Malaysia, 81310 Johor-Bahru, Malaysia; 3) Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 Johor-Bahru, Malaysia

DOI:

https://doi.org/10.14719/pst.2020.7.2.730

Keywords:

protein profiling, 1D SDS-PAGE

Abstract

Moringa oleifera is widely known as multipurpose tree since all of its parts confer multiple functions. The leaf is highly favourable among consumers while the petiole is mostly wasted. There are numerous studies on the flavonoid and antioxidant property of the stem and twig. However, study on the petiole has never been done. There-upon, this study was conducted to develop protein profiling of the petiole. In this study, 6 different protein extraction methods were tested on the fresh petiole before its protein quantity and quality were checked via Bradford assay and Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) respectively. The in-solution digestion was then done prior to LC-MS/MS analysis. The protein electrophoretic pattern from the SDS-PAGE proves that method 6 using Tris HCl buffer with incorporation of dithiothreitol (DTT) and phenylmethylsulfonyl fluoride (PMSF) confers the best quality of protein. It produced the highest number of visible individual bands compared to other methods. Meanwhile, 93 proteins were successfully identified via LCMS analysis where the protein, signal response and carbohydrate metabolism categories confer the highest percentage. High quality and content of the protein extracted from the petiole including the antioxidant, anticancer and antidiabetic protein identified suggested that consuming this part of the plant could enhance nutrients of human body.

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Author Biography

Zaidah Rahmat, 1) Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor-Bahru, Malaysia; 2) Chemical Management Unit, University Laboratory Management Centre (PPMU),Universiti Teknologi Malaysia, 81310 Johor-Bahru, Malaysia; 3) Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 Johor-Bahru, Malaysia

Education:

  • PhD (Plant Molecular Biology & Proteomics), Imperial College London, UK (2012)
  • MSc (Plant Biotechnology), Universiti Putra Malaysia, Malaysia (2001)
  • BSc (Hons Biotechnology), Universiti Putra Malaysia, Malaysia (1999)

Google scholar ID: http://scholar.google.com/citations?user=Xz7Ki5YAAAAJ&hl=en

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Published

19-04-2020

How to Cite

1.
Zulkifli ZA, Rahmat Z. Evaluation of effective protein extraction procedure to profile petiole of Moringa oleifera. Plant Sci. Today [Internet]. 2020 Apr. 19 [cited 2024 Nov. 21];7(2):214-8. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/730

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Section

Research Articles