Growth promoting properties of Mycobacterium and Bacillus on rice plants under induced drought

Authors

  • Joydip Karmakar Rice Biotechnology laboratory, Department of Biotechnology, Visva–Bharati, Santiniketan– 731 235, West Bengal, India http://orcid.org/0000-0002-8264-7654
  • Sayani Goswami Rice Biotechnology laboratory, Department of Biotechnology, Visva–Bharati, Santiniketan– 731 235, West Bengal, India
  • Krishnendu Pramanik Department of Botany, Visva-Bharati, Santiniketan– 731 235, West Bengal, India
  • Tushar Kanti Maiti Department of Botany, The University of Burdwan, Burdwan– 713 104, West Bengal, India
  • Rup Kumar Kar Department of Botany, Visva-Bharati, Santiniketan– 731 235, West Bengal, India
  • Narottam Dey Rice Biotechnology laboratory, Department of Biotechnology, Visva–Bharati, Santiniketan– 731 235, West Bengal, India

DOI:

https://doi.org/10.14719/pst.2021.8.1.965

Keywords:

Rice, Drought, PGPR, 16S rDNA, Mycobacterium sp., Bacillus sp.

Abstract

Management of drought stress through application of plant growth promoting rhizobacteria (PGPR) is now considered as an effective strategy in the present scenario of altered environmental conditions of the world. The aims and objectives of the present investigation was isolation, characterization and identification of some potential microbial resources (PGPR) from drought-affected upland rice fields of South Bengal followed by experimentation on the effect of isolated PGPR on drought induced rice seedlings. Selected isolates were isolated from rice field rhizospheric soils and tested for their PGPR activity, through phosphate solubilization, nitrogen fixation, IAA production and ACC deaminase activity. Then, the selected isolates were identified through 16S rDNA sequencing and phylogenetic analysis. Among the screened isolates two (isolate 1 and isolate 6) showing plant growth promoting traits, was applied on studied germplasm (IR64) to find out the influence of the applied organisms on rice growth and development under induced drought. Both organisms showed a positive influence (through increase in germination percentage, root growth, shoot growth, fresh weight and dry weight) on the studied rice growth and development under induced drought. Isolate 1 and 6 identified as species of Mycobacterium sp and Bacillus sp respectively through molecular taxonomy. Hence, these two isolates are expected to alleviate drought stress in the rice field for their nature of plant growth promotion under drought stress.

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Author Biographies

Joydip Karmakar, Rice Biotechnology laboratory, Department of Biotechnology, Visva–Bharati, Santiniketan– 731 235, West Bengal, India

Dr. Joydip Karmakar is a young researcher doing his job efficiently on Molecular Biology, Rice Genetics, Microbiology, Abiotic stress management etc.

Sayani Goswami, Rice Biotechnology laboratory, Department of Biotechnology, Visva–Bharati, Santiniketan– 731 235, West Bengal, India

Dr. Sayani Goswami is a young researcher doing her job efficiently on Molecular Biology, Rice Genetics, PGPR, Abiotic stress management etc.

Krishnendu Pramanik, Department of Botany, Visva-Bharati, Santiniketan– 731 235, West Bengal, India

Dr. Krishnendu Pramanik is a young researcher doing his job efficiently on PGPR, Microbiology, Abiotic stress management etc.

Tushar Kanti Maiti, Department of Botany, The University of Burdwan, Burdwan– 713 104, West Bengal, India

Prof. Tushar Kanti Maiti is a renowned worker in his field of research (Microbiology, PGPR, Molecular Biology etc).

Rup Kumar Kar, Department of Botany, Visva-Bharati, Santiniketan– 731 235, West Bengal, India

Dr. Narottam Dey, passionately working on Rice Genetics, PGPR, Plant Molecular Biology, Bioinformatics, Abiotic Stress Management, Rice Hybridization, QTL Mapping etc.

Narottam Dey, Rice Biotechnology laboratory, Department of Biotechnology, Visva–Bharati, Santiniketan– 731 235, West Bengal, India

Dr. Narottam Dey, passionately working on Rice Genetics, PGPR, Plant Molecular Biology, Bioinformatics, Abiotic Stress Management, Rice Hybridization, QTL Mapping etc.

References

Vinocur B, Altman A. Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Current Opinion Biotechnol. 2005;16(2):12–132. https://doi.org/10:1016/j.copbio.2005.02.001

Satterthwaite D, McGranahan G, Tacoli C. Urbanization and its implications for food and farming. Philos Trans R Soc Lond B Biol.Sci.2010;365(1554):2809–20. https://doi.org/10.1098/rstb.2010.0136

Laxa M, Liebthal M, Telman W, Chibani K, Dietz KJ. The role of the plant antioxidant system in drought tolerance. Antioxidants. 2019;8(4):94. https://doi.org/10.3390/antiox8040094

Oladosu Y, Rafii MY, Samuel C, Fatai A, Magaji U, Kareem I, Kamarudin ZS, Muhammad I, Kolapo K. Drought resistance in rice from conventional to molecular breeding: A Review. Int J Mol Sci. 2019;20(14):3519. https://doi.org/10.3390/ijms20143519

Sahebi M, Hanafi MM, Rafi MY, Mahmud TMM, Azizi P, Osman M, Abiri R, Taheri S, Kalhori N, Shabanimofrad M, Miah G, Atabaki N. Improvement of drought tolerance in rice (Oryza sativa L.): genetics, genomic tools, and the WRKY gene family. Biomed Res Int. 2018;2018:3158474. https://doi.org/10.1155/2018/3158474

Dixit S, Singh A, Kumar A. Rice breeding for high grain yield under drought: a strategic solution to a complex problem. Int J Agron. 2014, Article ID 863683. https://doi.org/10.1155/2014/863683

Vurukonda SSKP, Vardharajula S, Shrivastava M, Ali SkZ. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiol Res. 2016;184:13–24.

Hanks JH, Weintraub RL. The pure culture isolation of ammoniaoxidizing bacteria. Department of Bacteriology, School of Medicine and Department of Botany, The George Washington University, Washington, D. C. 1936.

Pikovskaya RI. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiologiya. 1948;17:362–70.

Premono EM, Moawad AM, Vlek PLG. Effect of phosphate-solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indones J Crop Sci. 1996;11:13–23.

Subba–Rao NS. Soil microorganisms and plant growth, 4th ed. In: Science Publishers Inc., USA; 1999.

Davis BD, Mingioli ES. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950;60:17–28.

Gordon SA, Weber RP. Colorimetric estimation of indoleaceticacid. Plant Physiol. 1951;26:192–97.

Glick BR, Karaturovic D, Newell P. A novel procedure for rapid isolation of plant growth promoting rhizobacteria. Can J Microbiol. 1995;41:533–36.

Dworkin M, Foster JW. Experiments with some microorganisms which utilize ethane and hydrogen. J Bacteriol. 1958;75:592–601.

Honma M, Shimomura T. Metabolism of 1-aminocyclopropane-1 carboxylic acid. Agric Biol Chem. 1978;42:1825–31.

Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem. 1976;72:248–54.

Galkiewicz JP, Kellogg CA. Cross–kingdom amplification using bacteria–specific primers: complications for studies of coral microbial echology. Appl Environ Microbiol. 2008;74:7828–31. https://doi.org/10.1128/AEM.01303–08

Jukes TH, Cantor CR. Evolution of protein molecules. In: Munro HN, editor. Mammalian protein metabolism. New York: Academic Press, 1969. p. 21–132.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol Biol Evol. 2013;30:2725–29.

Money NP. Osmotic pressure of aqueous polyethylene glycols: Relationship between molecular weight and vapor pressure deficit. Plant Physiol. 1989;91:766–69.

Duncan DB. Multiple range and multiple F tests. Biometrics. 1995;11:1–42.

Glick BR, Penrose DM and Li J. A model for lowering plant ethylene concentration by plant growth promoting rhizobacteria. J Theo Biol. 1998;190:63–68.

Vessey JK Plant growth promoting rhizobacteria as biofertilizers. Plant Soil. 2003;255:571–86.

Zahir AZ, Arshad M and Frankenberger WT Jr. Plant growth promoting rhizobacteria: application and perspectives in agriculture. Adv Agron. 2004;81:97–168.

Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA and Young CC Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl Soil Ecol. 2006;34:33–41.

Kim Y, Chung YS, Lee E, Tripathi P, Heo S, Kim KH. Root response to drought stress in rice (Oryza sativa L.). Int J Mol Sci. 2020;21:1–22.

Published

01-01-2021

How to Cite

1.
Karmakar J, Goswami S, Pramanik K, Maiti TK, Kar RK, Dey N. Growth promoting properties of Mycobacterium and Bacillus on rice plants under induced drought. Plant Sci. Today [Internet]. 2021 Jan. 1 [cited 2024 Nov. 21];8(1):49-57. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/965

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Section

Research Articles