Cellular response of oxidative stress when sub1A QTL of rice receives water deficit stress
DOI:
https://doi.org/10.14719/pst.2018.5.3.387Keywords:
sub1A QTL, rice, antioxidants, water deficit stress, ROSAbstract
In this experiment, sub1A quantitative trait loci (sub1A QTL) of rice were evaluated for dehydration responses through different aspects of cellular responses. Through variations of dehydration exposure, rice seedlings recorded a significant increase in superoxide (O2.-) and hydrogen peroxide (H2O2), the former by 1.80 fold and the latter by 2.10 fold. Nicotinamide adenine dinucleotide phosphate oxidase activity fairly correlated with lipid peroxidation (r = 1.96). Both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) had similar IC50 values over the control at p ? 0.05. Reducing power of the extract had 1.31 fold increase. The antioxidant moieties like total phenolics and flavonoids were 1.04 and 1.23 fold upregulated under stress. On the other hand anthocyanin and glutathione (GSH) did not vary much under stress except at maximum duration of stress. Superoxide dismutase (SOD) was initially stable but maximized at 8 days by 1.30 fold increase. On the contrary, guaiacol peroxidase (GPX) was seen to be downregulated by 40.94% all through the days of stress. Catalase (CAT) activity followed a similar trend, but was not significant as compared to control.
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5. Sarkar B, De AK, Adak MK. Physiological characterization of SUB1 trait in rice under subsequent submergence and re-aeration with interaction of chemical elicitors. Plant Science Today. 2017 Nov 27;4(4):177-90. https://doi.org/10.14719/pst.2017.4.4.351
6. Ismail AM, Singh US, Singh S, Dar MH, Mackill DJ. The contribution of submergence-tolerant (Sub1) rice varieties to food security in flood-prone rainfed lowland areas in Asia. Field Crops Research. 2013 ;152:83-93. https://doi.org/10.1016/j.fcr.2013.01.007
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8. Elstner EF, Heupel A. Inhibition of nitrite formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase. Analytical biochemistry. 1976 Feb 1;70(2):616-20. https://doi.org/10.1016/0003-2697(76)90488-7
9. Ghosh N, Adak MK, Ghosh PD, Gupta S, Gupta DS, Mandal C. Differential responses of two rice varieties to salt stress. Plant Biotechnology Reports. 2011 Jan 1;5(1):89-103. https://doi.org/10.1007/s11816-010-0163-y
10. Ishida A, Ookubo K, Ono K. Formation of hydrogen peroxide by NAD (P) H oxidation with isolated cell wall-associated peroxidase from cultured liverwort cells, Marchantia polymorpha L. Plant and cell physiology. 1987 Jun 1;28(4):723-6.
11. Heath RL, Packer L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of biochemistry and biophysics. 1968 Apr 1;125(1):189-98. https://doi.org/10.1016/0003-9861(68)90654-1
12. Dey P, Chaudhuri D, Chaudhuri TK, Mandal N. Comparative assessment of the antioxidant activity and free radical scavenging potential of different parts of Nerium indicum. International Journal of Phytomedicine. 2012 Jan 1;4(1):54.
13. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine. 1999 May 1;26(9-10):1231-7. https://doi.org/10.1016/S0891-5849(98)00315-3
14. Almulaiky YQ, Alshawafi WM, Al-Talhi HA, Zeyadi M, Anwar F, Al-abbasi FA, Khan R, Kumar V. Evaluation of the Antioxidant Potential and Antioxidant Enzymes of Some Yemeni Grape Cultivars. Free Radicals & Antioxidants. 2017 Jan 1;7(1).
15. Ferreira IC, Baptista P, Vilas-Boas M, Barros L. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food chemistry. 2007 Jan 1;100(4):1511-6. https://doi.org/10.1016/j.foodchem.2005.11.043
16. Mohsen SM, Ammar AS. Total phenolic contents and antioxidant activity of corn tassel extracts. Food chemistry. 2009 Feb 1;112(3):595-8. https://doi.org/10.1016/j.foodchem.2008.06.014
17. Basu S, Roychoudhury A, Saha PP, Sengupta DN. Differential antioxidative responses of indica rice cultivars to drought stress. Plant Growth Regulation. 2010 Jan 1;60(1):51. https://doi.org/10.1007/s10725-009-9418-4
18. Giusti MM, Wrolstad RE. Characterization and measurement of anthocyanins by UV?visible spectroscopy. Current protocols in food analytical chemistry. 2001 Aug.
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20. Cakmak I, Marschner H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant physiology. 1992 Apr 1;98(4):1222-7. https://doi.org/10.1104/pp.98.4.1222
21. Verma S, Dubey RS. Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Science. 2003 Apr 30;164(4):645-55. https://doi.org/10.1016/S0168-9452(03)00022-0
22. Olk DC, Cassman KG, Randall EW, Kinchesh P, Sanger LJ, Anderson JM. Changes in chemical properties of organic matter with intensified rice cropping in tropical lowland soil. European Journal of Soil Science. 1996 Sep 1;47(3):293-303. https://doi.org/10.1111/j.1365-2389.1996.tb01403.x
23. Banerjee S, Ghosh N, Mandal C, Dey N, Adak MK. Physiological basis of submergence tolerance in rice genotypes with reference to carbohydrate metabolism. Plant Gene and Trait. 2015 Apr 30;6.
24. Banerjee S, Dey N, Adak MK. Assessment of some biomarkers under submergence stress in some rice cultivars varying in responses. American Journal of Plant Sciences. 2015 Jan 6;6(01):84. https://doi.org/10.4236/ajps.2015.61010
25. Cruz de Carvalho MH. Drought stress and reactive oxygen species: production, scavenging and signaling. Plant Signaling & Behavior. 2008 Mar 1;3(3):156-65. https://doi.org/10.4161/psb.3.3.5536
26. Du H, Wang N, Cui F, Li X, Xiao J, Xiong L. Characterization of the ?-carotene hydroxylase gene DSM2 conferring drought and oxidative stress resistance by increasing xanthophylls and abscisic acid synthesis in rice. Plant Physiology 2010;154(3):1304-18. https://doi.org/10.1104/pp.110.163741
27. Leopoldini M, Rondinelli F, Russo N, Toscano M. Pyranoanthocyanins: a theoretical investigation on their antioxidant activity. Journal of agricultural and food chemistry. 2010 Jul 13;58(15):8862-71. https://doi.org/10.1021/jf101693k
28. Soga T, Baran R, Suematsu M, Ueno Y, Ikeda S, Sakurakawa T, Kakazu Y, Ishikawa T, Robert M, Nishioka T, Tomita M. Differential metabolomics reveals ophthalmic acid as an oxidative stress biomarker indicating hepatic glutathione consumption. Journal of Biological Chemistry. 2006 Jun 16;281(24):16768-76. https://doi.org/10.1074/jbc.M601876200
29. Arbona V, Manzi M, Zandalinas SI, Vives-Peris V, Pérez-Clemente RM, Gómez-Cadenas A. Physiological, metabolic, and molecular responses of plants to abiotic stress. InStress Signaling in Plants: Genomics and Proteomics Perspective, Volume 2 2017 (pp. 1-35). Springer, Cham.
30. Ghosh N, Das SP, Mandal C, Gupta S, Das K, Dey N, Adak MK. Variations of antioxidative responses in two rice cultivars with polyamine treatment under salinity stress. Physiology and Molecular Biology of Plants. 2012 Oct 1;18(4):301-13. https://doi.org/10.1007/s12298-012-0124-8
31. Lopez-Martinez LX, Oliart-Ros RM, Valerio-Alfaro G, Lee CH, Parkin KL, Garcia HS. Antioxidant activity, phenolic compounds and anthocyanins content of eighteen strains of Mexican maize. LWT-Food Science and Technology. 2009 Jul 31;42(6):1187-92. https://doi.org/10.1016/j.lwt.2008.10.010
32. Sarkar RK, Bhattacharjee B. Rice genotypes with SUB1 QTL differ in submergence tolerance, elongation ability during submergence and re-generation growth at re-emergence. Rice. 2011 Dec 1;5(1):7. https://doi.org/10.1007/s12284-011-9065-z
33. Lu Y, Li Y, Zhang J, Xiao Y, Yue Y, Duan L, Zhang M, Li Z. Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.). PLoS One. 2013 Jan 10;8(1):e52126. https://doi.org/10.1371/journal.pone.0052126
34. Schmitz AJ, Folsom JJ, Jikamaru Y, Ronald P, Walia H. SUB1A?mediated submergence tolerance response in rice involves differential regulation of the brassinosteroid pathway. New Phytologist. 2013 Jun 1;198(4):1060-70. https://doi.org/10.1111/nph.12202
35. Noctor G, Mhamdi A, Foyer CH. Oxidative stress and antioxidative systems: recipes for successful data collection and interpretation. Plant, cell & environment. 2016 May 1;39(5):1140-60. https://doi.org/10.1111/pce.12726
36. Nahar K, Hasanuzzaman M, Ahamed KU, Hakeem KR, Ozturk M, Fujita M. Plant responses and tolerance to high temperature stress: Role of exogenous phytoprotectants. InCrop production and global environmental issues 2015 (pp. 385-435). Springer, Cham.
37. Sarkar RK, Ray A. Submergence-tolerant rice withstands complete submergence even in saline water: Probing through chlorophyll a fluorescence induction OJIP transients. Photosynthetica 2016 Jun 1;54(2):275-87. https://doi.org/10.1007/s11099-016-0082-4
2. Bona C, de Chiara Moço MC, Mastroberti AA. Cytological aspects during the stretching of collapsed cells in the root aerenchyma of Potamogeton polygonus Cham. & Schltdl. (Potamogetonaceae). Flora. 2017 Nov 11.
3. Gautam P, Lal B, Tripathi R, Baig MJ, Shahid M, Maharana S, Bihari P, Nayak AK. Impact of Seedling Age and Nitrogen Application on Submergence Tolerance of Sub1 and Non-Sub1 Cultivars of Rice (Oryza sativa L.). Journal of Plant Growth Regulation. 2017:1-4. https://doi.org/10.1007/s00344-016-9661-7
4. Praba ML, Cairns JE, Babu RC, Lafitte HR. Identification of physiological traits underlying cultivar differences in drought tolerance in rice and wheat. Journal of Agronomy and Crop Science. 2009 Feb 1;195(1):30-46. https://doi.org/10.1111/j.1439-037X.2008.00341.x
5. Sarkar B, De AK, Adak MK. Physiological characterization of SUB1 trait in rice under subsequent submergence and re-aeration with interaction of chemical elicitors. Plant Science Today. 2017 Nov 27;4(4):177-90. https://doi.org/10.14719/pst.2017.4.4.351
6. Ismail AM, Singh US, Singh S, Dar MH, Mackill DJ. The contribution of submergence-tolerant (Sub1) rice varieties to food security in flood-prone rainfed lowland areas in Asia. Field Crops Research. 2013 ;152:83-93. https://doi.org/10.1016/j.fcr.2013.01.007
7. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum. 1962 Jul 1;15(3):473-97. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
8. Elstner EF, Heupel A. Inhibition of nitrite formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase. Analytical biochemistry. 1976 Feb 1;70(2):616-20. https://doi.org/10.1016/0003-2697(76)90488-7
9. Ghosh N, Adak MK, Ghosh PD, Gupta S, Gupta DS, Mandal C. Differential responses of two rice varieties to salt stress. Plant Biotechnology Reports. 2011 Jan 1;5(1):89-103. https://doi.org/10.1007/s11816-010-0163-y
10. Ishida A, Ookubo K, Ono K. Formation of hydrogen peroxide by NAD (P) H oxidation with isolated cell wall-associated peroxidase from cultured liverwort cells, Marchantia polymorpha L. Plant and cell physiology. 1987 Jun 1;28(4):723-6.
11. Heath RL, Packer L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of biochemistry and biophysics. 1968 Apr 1;125(1):189-98. https://doi.org/10.1016/0003-9861(68)90654-1
12. Dey P, Chaudhuri D, Chaudhuri TK, Mandal N. Comparative assessment of the antioxidant activity and free radical scavenging potential of different parts of Nerium indicum. International Journal of Phytomedicine. 2012 Jan 1;4(1):54.
13. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine. 1999 May 1;26(9-10):1231-7. https://doi.org/10.1016/S0891-5849(98)00315-3
14. Almulaiky YQ, Alshawafi WM, Al-Talhi HA, Zeyadi M, Anwar F, Al-abbasi FA, Khan R, Kumar V. Evaluation of the Antioxidant Potential and Antioxidant Enzymes of Some Yemeni Grape Cultivars. Free Radicals & Antioxidants. 2017 Jan 1;7(1).
15. Ferreira IC, Baptista P, Vilas-Boas M, Barros L. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food chemistry. 2007 Jan 1;100(4):1511-6. https://doi.org/10.1016/j.foodchem.2005.11.043
16. Mohsen SM, Ammar AS. Total phenolic contents and antioxidant activity of corn tassel extracts. Food chemistry. 2009 Feb 1;112(3):595-8. https://doi.org/10.1016/j.foodchem.2008.06.014
17. Basu S, Roychoudhury A, Saha PP, Sengupta DN. Differential antioxidative responses of indica rice cultivars to drought stress. Plant Growth Regulation. 2010 Jan 1;60(1):51. https://doi.org/10.1007/s10725-009-9418-4
18. Giusti MM, Wrolstad RE. Characterization and measurement of anthocyanins by UV?visible spectroscopy. Current protocols in food analytical chemistry. 2001 Aug.
19. Li F, Wang J, Ma C, Zhao Y, Wang Y, Hasi A, Qi Z. Glutamate receptor-like channel3. 3 is involved in mediating glutathione-triggered cytosolic calcium transients, transcriptional changes, and innate immunity responses in Arabidopsis. Plant physiology. 2013 Jul 1;162(3):1497-509. https://doi.org/10.1104/pp.113.217208
20. Cakmak I, Marschner H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant physiology. 1992 Apr 1;98(4):1222-7. https://doi.org/10.1104/pp.98.4.1222
21. Verma S, Dubey RS. Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Science. 2003 Apr 30;164(4):645-55. https://doi.org/10.1016/S0168-9452(03)00022-0
22. Olk DC, Cassman KG, Randall EW, Kinchesh P, Sanger LJ, Anderson JM. Changes in chemical properties of organic matter with intensified rice cropping in tropical lowland soil. European Journal of Soil Science. 1996 Sep 1;47(3):293-303. https://doi.org/10.1111/j.1365-2389.1996.tb01403.x
23. Banerjee S, Ghosh N, Mandal C, Dey N, Adak MK. Physiological basis of submergence tolerance in rice genotypes with reference to carbohydrate metabolism. Plant Gene and Trait. 2015 Apr 30;6.
24. Banerjee S, Dey N, Adak MK. Assessment of some biomarkers under submergence stress in some rice cultivars varying in responses. American Journal of Plant Sciences. 2015 Jan 6;6(01):84. https://doi.org/10.4236/ajps.2015.61010
25. Cruz de Carvalho MH. Drought stress and reactive oxygen species: production, scavenging and signaling. Plant Signaling & Behavior. 2008 Mar 1;3(3):156-65. https://doi.org/10.4161/psb.3.3.5536
26. Du H, Wang N, Cui F, Li X, Xiao J, Xiong L. Characterization of the ?-carotene hydroxylase gene DSM2 conferring drought and oxidative stress resistance by increasing xanthophylls and abscisic acid synthesis in rice. Plant Physiology 2010;154(3):1304-18. https://doi.org/10.1104/pp.110.163741
27. Leopoldini M, Rondinelli F, Russo N, Toscano M. Pyranoanthocyanins: a theoretical investigation on their antioxidant activity. Journal of agricultural and food chemistry. 2010 Jul 13;58(15):8862-71. https://doi.org/10.1021/jf101693k
28. Soga T, Baran R, Suematsu M, Ueno Y, Ikeda S, Sakurakawa T, Kakazu Y, Ishikawa T, Robert M, Nishioka T, Tomita M. Differential metabolomics reveals ophthalmic acid as an oxidative stress biomarker indicating hepatic glutathione consumption. Journal of Biological Chemistry. 2006 Jun 16;281(24):16768-76. https://doi.org/10.1074/jbc.M601876200
29. Arbona V, Manzi M, Zandalinas SI, Vives-Peris V, Pérez-Clemente RM, Gómez-Cadenas A. Physiological, metabolic, and molecular responses of plants to abiotic stress. InStress Signaling in Plants: Genomics and Proteomics Perspective, Volume 2 2017 (pp. 1-35). Springer, Cham.
30. Ghosh N, Das SP, Mandal C, Gupta S, Das K, Dey N, Adak MK. Variations of antioxidative responses in two rice cultivars with polyamine treatment under salinity stress. Physiology and Molecular Biology of Plants. 2012 Oct 1;18(4):301-13. https://doi.org/10.1007/s12298-012-0124-8
31. Lopez-Martinez LX, Oliart-Ros RM, Valerio-Alfaro G, Lee CH, Parkin KL, Garcia HS. Antioxidant activity, phenolic compounds and anthocyanins content of eighteen strains of Mexican maize. LWT-Food Science and Technology. 2009 Jul 31;42(6):1187-92. https://doi.org/10.1016/j.lwt.2008.10.010
32. Sarkar RK, Bhattacharjee B. Rice genotypes with SUB1 QTL differ in submergence tolerance, elongation ability during submergence and re-generation growth at re-emergence. Rice. 2011 Dec 1;5(1):7. https://doi.org/10.1007/s12284-011-9065-z
33. Lu Y, Li Y, Zhang J, Xiao Y, Yue Y, Duan L, Zhang M, Li Z. Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.). PLoS One. 2013 Jan 10;8(1):e52126. https://doi.org/10.1371/journal.pone.0052126
34. Schmitz AJ, Folsom JJ, Jikamaru Y, Ronald P, Walia H. SUB1A?mediated submergence tolerance response in rice involves differential regulation of the brassinosteroid pathway. New Phytologist. 2013 Jun 1;198(4):1060-70. https://doi.org/10.1111/nph.12202
35. Noctor G, Mhamdi A, Foyer CH. Oxidative stress and antioxidative systems: recipes for successful data collection and interpretation. Plant, cell & environment. 2016 May 1;39(5):1140-60. https://doi.org/10.1111/pce.12726
36. Nahar K, Hasanuzzaman M, Ahamed KU, Hakeem KR, Ozturk M, Fujita M. Plant responses and tolerance to high temperature stress: Role of exogenous phytoprotectants. InCrop production and global environmental issues 2015 (pp. 385-435). Springer, Cham.
37. Sarkar RK, Ray A. Submergence-tolerant rice withstands complete submergence even in saline water: Probing through chlorophyll a fluorescence induction OJIP transients. Photosynthetica 2016 Jun 1;54(2):275-87. https://doi.org/10.1007/s11099-016-0082-4
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01-07-2018
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Saha I, De AK, Sarkar B, Ghosh A, Dey N, Adak MK. Cellular response of oxidative stress when sub1A QTL of rice receives water deficit stress. Plant Sci. Today [Internet]. 2018 Jul. 1 [cited 2024 Apr. 28];5(3):84-9. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/387
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