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Plant Science Today (PST)

Research Articles

Vol. 13 No. sp1 (2026): Recent Advances in Agriculture

Chemical profiling of maize volatiles based on retention characteristics and dependent emission patterns of healthy, oviposition-induced and herbivore-induced plant volatiles affected by fall armyworm Spodoptera frugiperda (J.E. Smith)

DOI
https://doi.org/10.14719/pst.10056
Submitted
14 June 2025
Published
08-04-2026

Abstract

Maize (Zea mays L.) is a critically important global cereal crop, the productivity of which is increasingly threatened by the invasive fall armyworm, Spodoptera frugiperda. In response to herbivory, plants deploy indirect defences by emitting herbivore-induced plant volatiles (HIPVs), which can attract natural enemies of the pest. This study investigated the volatile organic compound (VOC) profile of maize plants under three conditions: healthy, oviposition-induced and herbivore-induced by S. frugiperda. Using gas chromatography mass spectrometry (GC-MS), we identified and quantified 11 key VOCs across 6 chemical classes. The results revealed significant qualitative and quantitative differences in the volatile profiles among the treatments. Key compounds included the aldehydes pentanal (2,2-dimethyl) and nonanal; the ketone 1-(3-ethyloxiranyl) ethanone; the alcohol 3-buten-2-ol; several esters, most prominently oxalic acid, cyclohexyl propyl ester; and the terpenes d-limonene and β-caryophyllene. The distinct emission patterns of these VOCs, particularly the significant changes in specific terpenes and green leaf volatiles (GLVs), provide crucial insights into the chemical ecology of maize defence against S. frugiperda. These findings suggest that induced volatile blends could play a role in tritrophic interactions and offer a foundation for developing sustainable pest management strategies based on plant signalling.

References

  1. 1. Hu XT, Dong BC. Herbivory and nitrogen availability affect performance of an invader Alternanthera philoxeroides and its native congener Alternanthera sessilis. Flora. 2019;257:151412. https://doi.org/10.1016/j.flora.2019.05.011
  2. 2. Sparks AN. A review of the biology of the fall armyworm. Florida Entomol. 1979;62(2):82–7. https://doi.org/10.2307/3494083
  3. 3. Montezano DG, Specht A, Sosa-Gómez DR, Roque-Specht VF, Sousa-Silva JC, Paula-Moraes SV, et al. Host plants of Spodoptera frugiperda (Lepidoptera: Noctuidae) in the Americas. Afr Entomol. 2018;26(2):286–300. https://doi.org/10.4001/003.026.0286
  4. 4. Goergen G, Kumar PL, Sankung SB, Togola A, Tamò M. First report of outbreaks of the fall armyworm Spodoptera frugiperda (J E Smith) (Lepidoptera, Noctuidae), a new alien invasive pest in west and central africa. PLoS One. 2016;11(10):e0165632. https://doi.org/10.1371/journal.pone.0165632
  5. 5. Hoballah MEF, Turlings TCJ. Experimental evidence that plants under caterpillar attack may benefit from attracting parasitoids. Evol Ecol Res. 2001;3:553–65.
  6. 6. Tholl D, Hossain O, Weinhold A, Röse USR, Wei Q. Trends and applications in plant volatile sampling and analysis. Plant J. 2021;106(2):314–25. https://doi.org/10.1111/tpj.15176
  7. 7. Bruce TJ, Pickett JA. Perception of plant volatile blends by herbivorous insects – Finding the right mix. Phytochemistry. 2011;72(13):1605–11 https://doi.org/10.1016/j.phytochem.2011.04.011
  8. 8. Dicke M, Van Loon JJA, Soler R. Chemical complexity of volatiles from plants induced by multiple attack. Nat Chem Biol. 2009;5:317–24. https://doi.org/10.1038/nchembio.169
  9. 9. Maurya AK. Application of plant volatile organic compounds (VOCs) in agriculture. In: Rakshit A, Singh H, Singh A, Singh U, Fraceto L, editors. New frontiers in stress management for durable agriculture. Singapore: Springer; 2020. p. 369–88. https://doi.org/10.1007/978-981-15-1322-0_21
  10. 10. Scutareanu P, Bruin J, Posthumus MA, Drukker B. Constitutive and herbivore-induced volatiles in pear, alder and hawthorn trees. Chemoecology. 2003;13:63–74. https://doi.org/10.1007/s00049-002-0228-7
  11. 11. Scott ER. Indirect and interactive effects of climate and herbivory on tea metabolites. PhD [dissertation]. Medford (MA): Tufts University; 2020.
  12. 12. Turlings TCJ, Lengwiler UB, Bernasconi ML, Wechsler D. Timing of induced volatile emissions in maize seedlings. Planta. 1998;207:146–52. https://doi.org/10.1007/s004250050466
  13. 13. Dudareva N, Klempien A, Muhlemann JK, Kaplan I. Biosynthesis, function and metabolic engineering of plant volatile organic compounds. New Phytol. 2013;198(1):16-32. https://doi.org/10.1111/nph.12145
  14. 14. Schnee C, Köllner TG, Held M, Turlings TCJ, Gershenzon J, Degenhardt J. The products of a single maize sesquiterpene synthase form a volatile defense signal that attracts natural enemies of maize herbivores. Proc Natl Acad Sci USA. 2006;103(4):1129-34. https://doi.org/10.1073/pnas.0508027103
  15. 15. Rojas JC, Kolomiets MV, Bernal JS. Nonsensical choices? Fall armyworm moths choose seemingly best or worst hosts for their larvae, but neonate larvae make their own choices. PLoS One. 2018;13(5):e0197628. https://doi.org/10.1371/journal.pone.0197628
  16. 16. Ortiz-Carreon FR, Rojas JC, Cisneros J, Malo EA. Herbivore-induced volatiles from maize plants attract Chelonus insularis, an egg-larval parasitoid of the fall armyworm. J Chem Ecol. 2019;45:326-37. https://doi.org/10.1007/s10886-019-01051-x
  17. 17. Kamala Jayanthi PD, Woodcock CM, Caulfield J, Birkett MA, Bruce TJA. Isolation and identification of host cues from mango, Mangifera indica, that attract gravid female oriental fruit fly, Bactrocera dorsalis. J Chem Ecol. 2012;38:361-9 https://doi.org/10.1007/s10886-012-0093-y
  18. 18. Souza Silva LR, Canale MC, Magalhães DM, Spotti Lopes JR, Simões Bento JM. Maize bushy stunt phytoplasma changes the emission of maize volatiles and the chemotaxis of non-infected Dalbulus maidis (Hemiptera: Cicadellidae). Arthropod-Plant Interactions. 2025;19:35. https://doi.org/10.1007/s11829-025-10144-2
  19. 19. Fabian B, Sachse S. Experience-dependent plasticity in the olfactory system of Drosophila melanogaster and other insects. Front Cell Neurosci. 2023;17:1130091. https://doi.org/10.3389/fncel.2023.1130091
  20. 20. Mahanta DK, Komal J, Samal I, Bhoi TK, Kumar PVD, Mohapatra S, et al. Plant defense responses to insect herbivores through molecular signaling, secondary metabolites, and associated epigenetic regulation. Plant Environ Interact. 2025;6(1):e70035. https://doi.org/10.1002/pei3.70035
  21. 21. Farmer EE. Surface-to-air signals. Nature. 2001;411:854-6. https://doi.org/10.1038/35081189
  22. 22. De Lange ES, Laplanche D, Guo H, Xu W, Vlimant M, Erb M, et al. Spodoptera frugiperda caterpillars suppress herbivore-induced volatile emissions in maize. J Chem Ecol. 2020;46:344-60. https://doi.org/10.1007/s10886-020-01153-x
  23. 23. Gargi C, Kennedy JS, Jayabal TD. Olfactory response of Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) to the volatiles of healthy and herbivore damaged maize plants and their profiling. J Pharm Innov. 2021; 10(10S): 1061-1067.
  24. 24. Wang J, Yi T, Wang M, Wei J, Yan W, Wen Y, et al. Herbivore-induced maize volatiles: dual functions in repelling fall armyworm and attracting natural enemies. Pest Manag Sci. 2025;81(7):3674-84. https://doi.org/10.1002/ps.8735
  25. 25. Ngumbi EN, Ugarte CM. Flooding and herbivory interact to alter volatile organic compound emissions in two maize hybrids. J Chem Ecol. 2021;47(7):707-18. https://doi.org/10.1007/s10886-021-01286-7
  26. 26. Leroy N, Martin C, Arguelles Arias A, Cornélis JT, Verheggen FJ. If all else fails: impact of silicon accumulation in maize leaves on volatile emissions and oviposition site selection of Spodoptera exigua Hübner. J Chem Ecol. 2022;48:841-9. https://doi.org/10.1007/s10886-022-01386-y

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