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Plant Science Today (PST)

Review Articles

Vol. 13 No. sp1 (2026): Recent Advances in Agriculture

Bio-fortification in crops, recent approaches, challenges and future strategies: A review

DOI
https://doi.org/10.14719/pst.10511
Submitted
8 July 2025
Published
26-02-2026

Abstract

Micronutrient deficiencies such as folic acid, carotenoids, selenium (Se), zinc (Zn), iron (Fe) and iodine (I) affect individuals globally, posing significant challenges to human health and development and so become a major concern for the future. Involvement of modern practices such as plant breeding and genetic engineering in biofortification has become a sustainable answer for these deficiencies. This strategy is particularly beneficial for the health of low-income people with limited access to commercially fortified foods. With a one-time investment and the ability for farmers to propagate seeds at minimal cost, biofortification offers a promising avenue for long-term nutritional improvement. Recent advancements have seen the introduction of biofortified crop varieties through targeting various macro and micronutrients, antioxidants and other bioavailable components. Despite its potential, biofortified crops encounter obstacles related to development, distribution and consumer acceptance. Overcoming these challenges is crucial for optimizing the utilization of biofortified foods and achieving widespread impact in combating malnutrition and starvation worldwide.

References

  1. 1. Von Grebmer K, Saltzman A, Birol E, Wiesman D, Prasai N, Yin S, et al. Synopsis: 2014 global hunger index: The challenge of hidden hunger. Washington, DC: Intl Food Policy Res Inst; 2014. (Vol. 83). https://doi.org/10.2499/9780896299580
  2. 2. Vlaic RA, Mureșan CC, Muste S, Mureșan V, Pop A, Mureșan GPA. Boletus edulis mushroom flour-based wheat bread as innovative fortified bakery product. Bull UASVM Food Sci Technol. 2019;76(1):52–62. https://doi.org/10.15835/buasvmcn-fst:2018.0022
  3. 3. Velu G, Crespo Herrera L, Guzman C, Huerta J, Payne T, Singh RP. Assessing genetic diversity to breed competitive biofortified wheat with enhanced grain Zn and Fe concentrations. Front Plant Sci. 2019;9:1971. https://doi.org/10.3389/fpls.2018.01971
  4. 4. Kromann P, Valverde F, Alvarado S, Vélez R, Pisuña J, Potosí B, et al. Can Andean potatoes be agronomically biofortified with iron and zinc fertilizers? Plant Soil. 2017;411(1-2):1–18. https://doi.org/10.1007/s11104-016-3065-0
  5. 5. Buturi CV, Mauro RP, Fogliano V, Leonardi C, Giuffrida F. Mineral biofortification of vegetables as a tool to improve human diet. Foods. 2021;10(2):223. https://doi.org/10.3390/foods10020223
  6. 6. De Souza JZ, De Mello Prado R, Silva SL, Farias TP, Neto JG, Junior JS. Silicon leaf fertilization promotes biofortification and increases dry matter, ascorbate content, and decreases post-harvest leaf water loss of chard and kale. Commun Soil Sci Plant Anal. 2019;50(2):1–9. https://doi.org/10.1080/00103624.2018.1556288
  7. 7. Timpanaro G, Bellia C, Foti VT, Scuderi A. Consumer behaviour of purchasing biofortified food products. Sustainability. 2020;12(16):6297. https://doi.org/10.3390/su12166297
  8. 8. HarvestPlus. Disseminating orange-fleshed sweet potato: Findings from a HarvestPlus project in Mozambique and Uganda. Washington, DC: HarvestPlus; 2010.
  9. 9. White PJ, Broadley MR. Biofortifying crops with essential mineral elements. Trends Plant Sci. 2005;10(12):586–93. https://doi.org/10.1016/j.tplants.2005.10.001
  10. 10. Siwela M, Pillay K, Govender L, Lottering S, Mudau FN, Modi AT, et al. Biofortified crops for combating hidden hunger in South Africa: Availability, acceptability, micronutrient retention and bioavailability. Foods. 2020;9(6):815. https://doi.org/10.3390/foods9060815
  11. 11. Tang T, Xie H, Wang Y, Lü B, Liang J. The effect of sucrose and abscisic acid interaction on sucrose synthase and its relationship to grain filling of rice (Oryza sativa L.). J Exp Bot. 2009;60(9):2641–52. https://doi.org/10.1093/jxb/erp114
  12. 12. Fanzo J, Bellows AL, Spiker ML, Thorne-Lyman AL, Bloem MW. The importance of food systems and the environment for nutrition. Am J Clin Nutr. 2021;113(1):7–16. https://doi.org/10.1093/ajcn/nqaa313
  13. 13. Obrador A, Gonzalez D, Alvarez JM. Effect of inorganic and organic copper fertilizers on copper nutrition in Spinacia oleracea and on labile copper in soil. J Agric Food Chem. 2013;61(20):4692–701. https://doi.org/10.1021/jf305473f
  14. 14. Waters BM, Grusak MA. Quantitative trait locus mapping for seed mineral concentrations in two Arabidopsis thaliana recombinant inbred populations. New Phytol. 2008;179(4):1033–47. https://doi.org/10.1111/j.1469-8137.2008.02544.x
  15. 15. Chavez AL, Sánchez T, Jaramillo G, Bedoya JM, Echeverry J, Bolaños EA, et al. Variation of quality traits in cassava roots evaluated in landraces and improved clones. Euphytica. 2005;143(1):125–33. https://doi.org/10.1007/s10681-005-3057-2
  16. 16. Bouis HE, Hotz C, McClafferty B, Meenakshi JV, Pfeiffer WH. Biofortification: A new tool to reduce micronutrient malnutrition. Food Nutr Bull. 2011;32(Suppl 1):S31–40. https://doi.org/10.1177/15648265110321S105
  17. 17. Bouis HE, Saltzman A. Improving nutrition through biofortification: A review of evidence from HarvestPlus, 2003 through. Glob Food Secur. 2017;12:49–58. https://doi.org/10.1016/j.gfs.2017.01.009
  18. 18. Bouis HE, Welch RM. Biofortification: A sustainable agricultural strategy for reducing micronutrient malnutrition in the Global South. Crop Sci. 2010;50:20–32. https://doi.org/10.2135/cropsci2009.09.0531
  19. 19. Pérez-Massot E, Banakar R, Gómez-Galera S, Zorrilla-López U, Sanahuja G, Arjó G, et al. The contribution of transgenic plants to better health through improved nutrition: Opportunities and constraints. Genes Nutr. 2013;8:29–41.
  20. https://doi.org/10.1007/s12263-012-0315-5
  21. 20. Christou P, Twyman RM. The potential of genetically enhanced plants to address food insecurity. Nutr Res Rev. 2004;17(1):23–42. https://doi.org/10.1079/NRR200373
  22. 21. Kenz M, Stangoulis JCR. Calcium biofortification of crops - Challenges and projected benefits. Front Plant Sci. 2021;12. https://doi.org/10.3389/fpls.2021.669053
  23. 22. Siddique KH, Li X, Gruber K. Rediscovering Asia's forgotten crops to fight chronic and hidden hunger. Nat Plants. 2021;7(2):116-22. https://doi.org/10.1038/s41477-021-00850-z
  24. 23. da Silva DF, Cipriano PE, de Souza RR, Júnior MS, da Silva RF, Faquin V, et al. Anatomical and physiological characteristics of Raphanus sativus L. submitted to different selenium sources and forms application. Sci Hortic. 2020;260:108839. https://doi.org/10.1016/j.scienta.2019.108839
  25. 24. Barrameda-Medina Y, Leon MBB, Lentini M, Esposito S, Baenas N, Moreno DA, et al. Biofortification improves phytochemicals and amino-acidic profile in Brassica oleracea cv Bronco. Plant Sci. 2017;258:45-51. https://doi.org/10.1016/j.plantsci.2017.02.004
  26. 25. Pingali P, Sunder N. Transitioning toward nutrition-sensitive food systems in developing countries. Annu Rev Resour Econ. 2017;9:439-59. https://doi.org/10.1146/annurev-resource-100516-053552
  27. 26. D'Imperio M, Renna M, Cardinali A, Buttaro D, Serio F, Santamaria P. Biofortification and bioaccessibility in soilless "baby leaf" vegetable production. Food Chem. 2016;213:149-56. https://doi.org/10.1016/j.foodchem.2016.06.071
  28. 27. International Food Policy Research Institute. Analysis of CGIAR 2018 policy contributions: Overview and country-level insights. https://www.ifpri.org/publication/analysis-cgiar-2018-policy-contributions-overview-and-country-level-insights
  29. 28. Liang J, Li Z, Tsuji K, Nakano K, Nout MR, Hamer RJ. Milling characteristics and distribution of phytic acid and zinc in long-, medium- and short-grain rice. J Cereal Sci. 2008;48(1):83-91. https://doi.org/10.1016/j.jcs.2007.08.003
  30. 29. Calayugan MIC, Swamy BM, Nha CT, Palanog AD, Biswas PS, Descalsota-Empleo GIL, et al. Zinc-biofortified rice: a sustainable food-based product for fighting zinc malnutrition. In: Rice improvement: physiological, molecular breeding and genetic perspectives. Cham: Springer; 2021. p. 449-70. https://doi.org/10.1007/978-3-030-66530-2_13
  31. 30. De Sousa Lima F, Nascimento CWA, Da Silva Sousa C. Zinc fertilization as an alternative to increase the concentration of micronutrients in edible parts of vegetables. Rev Bras Cienc Agrar. 2015;10(3):403-8. https://doi.org/10.5039/agraria.v10i3a5132
  32. 31. McDowell LR. Minerals in animal and human nutrition. 2nd ed. Amsterdam: Elsevier Science BV; 2003.
  33. https://doi.org/10.1016/B978-0-444-51367-0.X5001-6
  34. 32. Natesan S, Duraisamy T, Pukalenthy B, Chandran S, Nallathambi J, Adhimoolam K, et al. Enhancing β-carotene concentration in parental lines of CO6 maize hybrid through marker-assisted backcross breeding (MABB). Front Nutr. 2020;7:134. https://doi.org/10.3389/fnut.2020.00134
  35. 33. Kyriacou MC, Rouphael Y. Towards a new definition of quality for fresh fruits and vegetables. Sci Hortic. 2018;234:463-9. https://doi.org/10.1016/j.scienta.2017.09.046
  36. 34. Hurrell RM. Factors associated with regular exercise. Percept Mot Skills. 1997;84(3):871-4. https://doi.org/10.2466/pms.1997.84.3.871
  37. 35. Roohani N, Hurrell R, Kelishadi R, Schulin R. Zinc and its importance for human health: An integrative review. J Res Med Sci. 2013;18(2):144.
  38. 36. Al-Babili S, Beyer P. Golden Rice-five years on the road-five years to go. Trends Plant Sci. 2005;10(12):565-73. https://doi.org/10.1016/j.tplants.2005.10.006
  39. 37. Skeaff SA. Iodine deficiency in pregnancy: The effect on neurodevelopment in the child. Nutrients. 2011;3(2):265-73. https://doi.org/10.3390/nu3020265
  40. 38. De Valença AW, Bake A, Brouwer ID, Giller KE. Agronomic biofortification of crops to fight hidden hunger in sub-Saharan Africa. Glob Food Secur. 2017;12:8-14. https://doi.org/10.1016/j.gfs.2016.12.001
  41. 39. Saltzman A, Birol E, Bouis HE, Boy E, De Moura FF, Islam Y, et al. Biofortification: Progress toward a more nourishing future. Glob Food Secur. 2013;2(1):9-17. https://doi.org/10.1016/j.gfs.2012.12.003
  42. 40. Diretto G, Tavazza R, Welsch R, Pizzichini D, Mourgues F, Papacchioli V, et al. Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase. BMC Plant Biol. 2006;6:1-11. https://doi.org/10.1186/1471-2229-6-13
  43. 41. Batis C, Marron-Ponce JA, Stern D, Vandevijvere S, Barquera S, Rivera JA. Adoption of healthy and sustainable diets in Mexico does not imply higher expenditure on food. Nat Food. 2021;2(10):792-801. https://doi.org/10.1038/s43016-021-00359-w
  44. 42. Bouis HE, Saltzman A, Birol E. Improving nutrition through biofortification. Agric Impro Nutr Seizing Momentum. 2019;47. https://doi.org/10.1079/9781786399311.0047
  45. 43. Juliano BO. Factors affecting nutritional properties of rice protein. Trans Natl Acad Sci Technol. 1985;7:205-16.
  46. 44. van Ginkel M, Cherfas J. What is wrong with biofortification. Glob Food Secur. 2023;37:100689. https://doi.org/10.1016/j.gfs.2023.100689
  47. 45. Buturi CV, Leonardi VFC, Giuffrida F. Mineral biofortification of vegetables as a tool to improve human diet. Foods. 2021;10(2):223. https://doi.org/10.3390/foods10020223
  48. 46. Castillo-Godina RG, Foroughbakhch-Pournavab R, Benavides-Mendoza A. Effect of selenium on elemental concentration and antioxidant enzymatic activity of tomato plants. J Agric Sci Technol. 2016;18:233-44.
  49. 47. White PJ, Broadley MR. Biofortification of crops with seven mineral elements often lacking in human diets: Iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytol. 2009;182(1):49-84. https://doi.org/10.1111/j.1469-8137.2008.02738.x
  50. 48. Montesano FF, D'Imperio M, Parente A, Cardinali A, Renna M, Serio F. Green bean biofortification for Si through soilless cultivation: Plant response and Si bioaccessibility in pods. Sci Rep. 2016;6:1-9. https://doi.org/10.1038/srep31662
  51. 49. Haas D, Défago G. Biological control of soil-borne pathogens by fluorescent pseudomonads. Nat Rev Microbiol. 2005;3(4):307-19. https://doi.org/10.1038/nrmicro1129
  52. 50. HarvestPlus. Biofortification: the evidence. A summary of research informing scaling up of biofortification to improve nutrition and health globally. Washington, DC: HarvestPlus; 2019.
  53. 51. Gómez-Galera S, Rojas E, Sudhakar D, Zhu C, Pelacho AM, Capell T, et al. Critical evaluation of strategies for mineral fortification of staple food crops. Transgenic Res. 2010;19:165-80. https://doi.org/10.1007/s11248-009-9311-y
  54. 52. Gregorio GB, Senadhira D, Htut H, Graham RD. Breeding for trace mineral density in rice. Food Nutr Bull. 2000;21(4):382-6. https://doi.org/10.1177/156482650002100407

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