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Plant Science Today (PST)

Research Articles

Vol. 13 No. sp1 (2026): Recent Advances in Agriculture

Impact of brassinosteroids on biochemical parameters of papaya (Carica papaya L. cv. Red Lady.) under salt stress

DOI
https://doi.org/10.14719/pst.10764
Submitted
20 July 2025
Published
13-02-2026

Abstract

In fruit orchards, the excessive use of NaCl caused significant plant physiological stress and a decline in soil health. In order to counteract the negative effects of salinity stress, the current study was carried out in 2024 to assess how foliar spraying with brassinosteroids (BRs) affected the rate of expansion and biochemical functioning of Carica papaya L. cv. Red Lady. In fruit orchards, brassinosteroid foliar sprays were applied at concentrations of 0.05, 0.1 and 0.2 mg L-1 in combination with salinity treatments of 150, 200 and 250 mM NaCl, along with an untreated control. A number of physiological and biochemical parameters were evaluated in the study, such as membrane stability index, injury index, anthocyanin and carotenoid contents, proline accumulation, catalase activity, total chlorophyll, chlorophyll a, chlorophyll b and chlorophyll a/b ratio. The application of brassinosteroid was found to significantly mitigate the adverse consequences of salinity stress, as evidenced by better pigment and osmolyte levels as well as increased antioxidant enzyme activity. Biochemical parameters improved most noticeably with 0.1 mg L-1 and 0.2 mg L-1 BR in combination with 200 mM NaCl among the treatments. According to the research, papaya’s ability to withstand salt can be successfully increased by foliar implementation of brassinosteroids, especially at 0.1 mg L-1, which improves antioxidative defence and preserves membrane stability.

References

  1. 1. Pandya YH, Bakshi M, Sharma A. Effect of calcium nitrate and calcium carbonate on plant growth, fruit quality and yield of Papaya Cv. Red Lady. Int J Agric Anim Prod. 2023;33(33):25-32. https://doi.org/10.55529/ijaap.33.25.32
  2. 2. Vyas D, Saket M. Effect of plant growth regulators on growth, yield and quality of Papaya cv. Red Lady under prayagrajagro climatic conditions. Int J Environ Clim Change. 2023;13(9):347-55.https://doi.org/10.9734/ijecc/2023/v13i92238
  3. 3. Yang Z, Liu Z, Ge X, Lu L, Qin W, Qanmber G, et al. Brassinosteroids regulate cotton fiber elongation by modulating very-long-chain fatty acid biosynthesis. Plant Cell. 2023;35(6):2114-31. https://doi.org/10.1093/plcell/koad060
  4. 4. Ren Y, Li X, Liang J, Wang S, Wang Z, Chen H, et al. Brassinosteroids and gibberellic acid actively regulate the zinc detoxification mechanism of Medicago sativa L. seedlings. BMC Plant Biol. 2023;23(1):75. https://doi.org/10.1186/s12870-023-04091-4
  5. 5. Gao M, Wang Z, Jia Z, Zhang H, Wang T. Brassinosteroids alleviate nanoplastic toxicity in edible plants by activating antioxidant defense systems and suppressing nanoplastic uptake. Environ Int. 2023;174:107901. https://doi.org/10.1016/j.envint.2023.107901
  6. 6. Liu J, Yang R, Jian N, Wei L, Ye L, Wang R, et al. Putrescine metabolism modulates the biphasic effects of brassinosteroids on canola and Arabidopsis salt tolerance. Plant Cell Environ. 2020;43(6):1348-59. https://doi.org/10.1111/pce.13757
  7. 7. Kumar P, Sharma PK. Soil salinity and food security in India. Front Sustain Food Syst. 2020;4:533781. https://doi.org/10.3389/fsufs.2020.533781
  8. 8. Vázquez MN, Guerrero YR, de la Noval WT, Gonzalez LM, Zullo MA. Advances on exogenous applications of brassinosteroids and their analogs to enhance plant tolerance to salinity: a review. Aust J Crop Sci. 2019;13(1):115-21. https://doi.org/10.21475/ajcs.19.13.01.p1404
  9. 9. Wu W, Zhang Q, Ervin EH, Yang Z, Zhang X. Physiological mechanism of enhancing salt stress tolerance of perennial ryegrass by 24-epibrassinolide. Front Plant Sci. 2017;8:1017. https://doi.org/10.3389/fpls.2017.01017
  10. 10. Yue J, You Y, Zhang L, Fu Z, Wang J, Zhang J, et al. Exogenous 24-epibrassinolide alleviates effects of salt stress on chloroplasts and photosynthesis in Robinia pseudoacacia L. seedlings. J Plant Growth Regul. 2019;38:669-82. https://doi.org/10.1007/s00344-018-9881-0
  11. 11. Ahanger MA, Mir RA, Alyemeni MN, Ahmad P. Combined effects of brassinosteroid and kinetin mitigates salinity stress in tomato through the modulation of antioxidant and osmolyte metabolism. Plant Physiol Biochem. 2020;147:31-42. https://doi.org/10.1016/j.plaphy.2019.12.007
  12. 12. Kutay ZA, Venkatesan A. Mitigation of salinity induced effects by foliar application of brassinosteroids in Vigna radiata L. Co (Gg) 7 variety. Indian J Nat Sci. 2022;12(70):38748-57. https://www.researchgate.net/publication/365705171
  13. 13. Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil. 1973;39(1):205-7. https://doi.org/10.1007/BF00018060
  14. 14. Aebi H. Catalase in vitro. In: Methods Enzymol. Vol 105. Academic Press. 1984. p. 121-6. https://doi.org/10.1016/S0076-6879(84)05016-3
  15. 15. Arnon DI. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 1949;24(1):1-15. https://doi.org/10.1104/pp.24.1.1
  16. 16. Mancinelli AL. Photoregulation of anthocyanin synthesis: VIII. Effect of light pretreatments. Plant Physiol. 1984;75(2):447-53. https://doi.org/10.1104/pp.75.2.447
  17. 17. Marková H, Tarkowská D, Čečetka P, Kočová M, Rothová O, Holá D. Contents of endogenous brassinosteroids and the response to drought and/or exogenously applied 24-epi brassinolide in two different maize leaves. Front Plant Sci. 2023;14:1139162. https://doi.org/10.3389/fpls.2023.1139162
  18. 18. Ali M, Kamran M, Abbasi GH, Saleem MH, Ahmad S, Parveen A, et al. Melatonin-induced salinity tolerance by ameliorating osmotic and oxidative stress in the seedlings of two tomato (Solanum lycopersicum L.) cultivars. J Plant Growth Regul. 2021;40:2236-48. https://doi.org/10.1007/s00344-020-10273-3
  19. 19. Rossatto T, do Amaral MN, Benitez LC, Vighi IL, Braga EJ, de Magalhaes Júnior AM, et al. Gene expression and activity of antioxidant enzymes in rice plants, cv. BRS AG, under saline stress. Physiol Mol Biol Plants. 2017;23:865-75. https://doi.org/10.1007/s12298-017-0467-2
  20. 20. Liu X, Zhu Q, Liu W, Zhang J. Exogenous brassinosteroid enhances zinc tolerance by activating the phenylpropanoid biosynthesis pathway in Citrullus lanatus L. Plant Signal Behav. 2023;18(1):2186640. https://doi.org/10.1080/15592324.2023.2186640
  21. 21. Tanveer M. Role of 24-Epibrassinolide in inducing thermo-tolerance in plants. J Plant Growth Regul. 2019;38(3):945-55. https://doi.org/10.1007/s00344-018-9904-x
  22. 22. Ghoname AA, AbdelMotlb NA, Abdel-Al FS, Abu El-Azm NA, Abd Elhady SA, Merah O, et al. Brassinosteroids or proline can alleviate yield inhibition under salt stress via modulating physio-biochemical activities and antioxidant systems in snap bean. J Hortic Sci Biotechnol. 2023;98(4):526-39. https://doi.org/10.1080/14620316.2022.2151518
  23. 23. de Oliveira VP, Lima MD, da Silva BR, Batista BL, da Silva Lobato AK. Brassinosteroids confer tolerance to salt stress in Eucalyptus urophylla plants enhancing homeostasis, antioxidant metabolism and leaf anatomy. J Plant Growth Regul. 2019;38:557-73. https://doi.org/10.1007/s00344-018-9870-3
  24. 24. Shafi A, Zahoor I, Mushtaq U. Proline accumulation and oxidative stress: Diverse roles and mechanism of tolerance and adaptation under salinity stress. In: Salt stress, microbes and plant interactions: mechanisms and molecular approaches. Vol 2. 2019. p. 269-300. https://doi.org/10.1007/978-981-13-8805-7_13
  25. 25. Ahmad P, Ahanger MA, Egamberdieva D, Alam P, Alyemeni MN, Ashraf M. Modification of osmolytes and antioxidant enzymes by 24-epibrassinolide in chickpea seedlings under mercury (Hg) toxicity. J Plant Growth Regul. 2018;37:309-22. https://doi.org/10.1007/s00344-017-9730-6
  26. 26. Ghosh UK, Islam MN, Siddiqui MN, Cao X, Khan MA. Proline, a multifaceted signalling molecule in plant responses to abiotic stress: understanding the physiological mechanisms. Plant Biol. 2022;24(2):227-39. https://doi.org/10.1111/plb.13363
  27. 27. Gupta S, Schillaci M, Walker R, Smith PM, Watt M, Roessner U. Alleviation of salinity stress in plants by endophytic plant-fungal symbiosis: Current knowledge, perspectives and future directions. Plant Soil. 2021;461:219-44. https://doi.org/10.1007/s11104-020-04618-w
  28. 28. Zhou X, Zhang N, Yang J, Tang X, Wen Y, Si H. Functional analysis of StDWF4 gene in response to salt stress in potato. Plant Physiol Biochem. 2018;125:63-73. https://doi.org/10.1016/j.plaphy.2018.01.027
  29. 29. Su Q, Zheng X, Tian Y, Wang C. Exogenous brassinolide alleviates salt stress in Malus hupehensis Rehd. by regulating the transcription of NHX-Type Na+ (K+)/H+ antiporters. Front Plant Sci. 2020;11:38. https://doi.org/10.3389/fpls.2020.00038
  30. 30. El-Banna MF, Al-Huqail AA, Farouk S, Belal BE, El-Kenawy MA, Abd El-Khalek AF. Morpho-physiological and anatomical alterations of salt-affected thompson seedless grapevine (Vitis vinifera L.) to brassinolide spraying. Horticulturae. 202;8(7):568. https://doi.org/10.3390/horticulturae8070568
  31. 31. Dong NQ, Lin HX. Contribution of phenylpropanoid metabolism to plant development and plant–environment interactions. J Integr Plant Biol. 2021;63(1):180-209. https://doi.org/10.1111/jipb.13054
  32. 32. Hassan MU, Guoqin H, Nawaz M, Shah AN, Khan TA, Haq MI, et al. The role of brassinosteroids in plant physiological and molecular responses to counter salt stress and ensure food security: A review and future perspectives. Turk J Agric For. 2025;49(1):1-23. https://doi.org/10.55730/1300-011X.3245
  33. 33. Song J, Sun B, Chen C, Ning Z, Zhang S, Cai Y, et al. An R-R-type MYB transcription factor promotes non-climacteric pepper fruit carotenoid pigment biosynthesis. Plant J. 2023;115(3):724-41. https://doi.org/10.1111/tpj.16257
  34. 34. Huang JJ, Lin S, Xu W, Cheung PC. Occurrence and biosynthesis of carotenoids in phytoplankton. Biotechnol Adv. 2017;35(5):597-618. https://doi.org/10.1016/j.biotechadv.2017.05.001

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