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Plant Science Today (PST)

Research Articles

Vol. 8 No. 2 (2021)

Antibacterial, antioxidant and cytotoxic activities of different fractions of acetone extract from flowers of Dipterocarpus intricatus Dyer (Dipterocarpaceae)

DOI
https://doi.org/10.14719/pst.2021.8.2.1086
Submitted
5 January 2021
Published
01-04-2021

Abstract

This study has shown for the first time the antimicrobial, antioxidant and cytotoxicity of 3 fractions of acetone extract, including hexane, chloroform and ethyl acetate from flowers of Dipterocarpus intricatus. Antibacterial test using disc diffusion method showed that the chloroform and ethyl acetate fractions inhibited the growth of all the tested bacteria, including Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium, Bacillus cereus and Staphylococcus aureus while the hexane fraction showed the antibacterial activity against B. cereus and S. enteritidis. Antioxidant activity and cancer cell resistance of those extracts were conducted using DPPH and MTT methods respectively. As a result, the DPPH radical scavenging activity of the hexane, chloroform and ethyl acetate fractions were determined with the IC50 values of 0.508, 0.22 and 0.075 mg/mL respectively while the cytotoxicity to HepG2 cell line of those fractions was 163.3 ppm, 106.7 ppm and 459.3 ppm. These results suggested the potential application of these fractions isolated from D. intricatus flowers as the natural antimicrobial, antioxidant and cytotoxic agents for medicine.

References

  1. Ashton P. Dipterocarpus baudii, The IUCN red list of threatened species. Available from: www. iucnredlist. Org, 2014.
  2. Pham HH, Cây c? Vi?t Nam: An Illustrated Flora of Vietnam. Youth Publishing House, Ho Chi Minh City, Vietnam, 2000.
  3. Wibowo A, Ahmat N, Hamzah AS, Ismail NH, Ahmad R, Jaafar FM. Resveratrol oligomers from the stem bark of Dryobalanops aromatica. Biochemical Systematics and Ecology. 2012;40:62-64. https://doi.org/10.1016/j.bse.2011.09.013
  4. Pauline DYP. Plants used in Cambodia. Self-published, printed by Imprimerie Olympic, Phnom Penh, 2000.
  5. Aslam MS, Ahmad MS, Mamat AS. A phytochemical, ethnomedicinal and pharmacological review of genus Dipterocarpus. International Journal of Pharmacy and Pharmaceutical Sciences. 2015;7(4):27-38.
  6. Appanah S, Turnbull JM. A Review of Dipterocarpus: Taxonomy, ecology and silviculture. Center for International Forestry Research, Bogor, 1998.
  7. Seo C, Ahn EK, Lee JA, Kang JS, Byun HW, Hong SS. Phenolic contituents of the stems of Dipterocarpus intricatus. Chemistry of Natural Compounds. 2020;56:920-22. https://doi.org/10.1007/s10600-020-03187-9
  8. Bobinaite R, Viskelis P, Sarkinas A, Venskutonis PR. Phytochemical composition, antioxidant and antimicrobial properties of raspberry fruit, pulp, and marc extract. Journal of Food.2013;11:334-42. https://doi.org/10.1080/19476337.2013.766265
  9. Clinical and Laboratory Standards Institute. Methods for Antimicrobial Dilution and Disk Susceptibility of Infrequently Isolated or Fastidious Bacteria, Approved Guideline, 2nd. ed., CLSI document M45-A2. Clinical and Laboratory Standards Institute, 950 West Valley Roadn Suite 2500,Wayne, Pennsylvania 19087, USA, 2010.
  10. Thaipong K, Boonprakob U, Crosby K, Cisneros ZL, Hawkins BD. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis. 2006;19:669-75. https://doi.org/10.1016/j.jfca.2006.01.003
  11. Hussain RF, Nouri AME, Oliver RTD. A new approach for measurement of cytotoxicity using colorimetric assay. Journal of Immunological Methods. 1993;160(1):89-96. https://doi.org/10.1016/0022-1759(93)90012-v
  12. Gupta AS, Dev S. Studies in sesquiterpenes—XLVI: Sesquiterpenes from the oleoresin of Dipterocarpus pilosus: Humulene epoxide-III, caryophyllenol-I and caryophyllenol-II. Tetrahedron. 1971;27(3):635-44. https://doi.org/10.1016/S0040-4020(01)90731-8
  13. Khiev P, Kwon OK, Song HH, Oh SR, Ahn KS, Lee HK. Cytotoxic terpenes from the stems of Dipterocarpus obtusifolius collected in Cambodia. Biological and Pharmaceutical Bulletin. 2012;60(8):955-61. https://doi.org/10.1248/cpb.c12-00012
  14. Barry AL, Coyle MB. Methods of measuring zones of inhibition with the Bauer-Kirby disk susceptibility test. Journal of Clinical Microbiology. 1979;10:8858-99.
  15. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199-1200.
  16. Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry. 1998;46:4113-4117. https://doi.org/10.1021/jf9801973
  17. Osawa T, Namiki M. A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves. Agricultural and Biological Chemistry. 1981;45(3):735-39. https://doi.org/10.1080/00021369.1981.10864583
  18. Chatuphonprasert W, Tatiya-aphiradee N, Thammawat S, Yongram C, Puthongking P, Jarukamjorn K. Antibacterial and wound healing activity of Dipterocarpus alatus Crude extract against methicillin-resistant Staphylococcus aureus-induced superficial skin infection in mice. Journal of Skin and Stem Cell. 6(1);e99579. https://doi.org/10.5812/jssc.99579.
  19. Biswas D, Mahalakshmi B, Samelu B, Devadas M, Suresehkumar SV. Antimicrobial activity of ethanol extracts and isolated fractions Shorea robusta Gaertn f and Dipterocarpus turbinatus Gaertn f. Pharmacognosy Communications. 2020;10(2):102-07. https://doi.org/ 10.5530/pc.2020.2.19
  20. Yongram C, Sungthong B, Puthongking P, Weerapreeyakul N. Chemical composition, antioxidant and cytotoxicity activities of leaves, bark, twigs and oleo-resin of Dipterocarpus alatus. Molecules. 2019;24(27):3083. https://doi.org/10.3390/molecules24173083
  21. Zain WZWM, Ahmat N, Osman CP. Antioxidant activities of oligostilbenoids from the stem bark of Dipterocarpus verrucosus, Dipterocarpus crinitus and Dipterocarpus cornutus. International Journal of Engineering & Technology. 2018;7(4): 409-14. http://dx.doi.org/10.14419/ijet.v7i4.18.21979
  22. Yongram C, Tadtong S, Weerapreeyakul N, Puthongking P. The preliminary study of Yang Na (Dipterocarpus alatus Roxb. ex G.Don) extracts on anticancer and antifungal activities and the HPLC analysis of the phenolic compounds. Journal of Thai Traditional and Alternative Medicine. 2019;17(3):437-46.
  23. Muhtadi, Euis H. Hakim, Juliawaty Ld, Syah YM, Achmad SA, Latip J, Ghisalberti EL. Cytotoxic resveratrol oligomers from the tree bark of Dipterocarpus hasseltii. Fitoterapia. 2006;77(7-8)550-55. https://doi.org/0.1016/j.fitote.2006.07.004

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