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Plant Science Today (PST)

Research Articles

Vol. 13 No. sp1 (2026): Recent Advances in Agriculture

Molecular characterisation of ToLCNDV isolates causing yellow mosaic disease in gourds of Karnataka

DOI
https://doi.org/10.14719/pst.11155
Submitted
6 August 2025
Published
13-02-2026

Abstract

Yellow mosaic disease (YMD) is a major constraint in cucurbit production in India. The present study was undertaken to detect and characterise begomoviruses associated with YMD in ridge gourd (Luffa acutangula (L.) Roxb.), bitter gourd (Momordica charantia L.), ivy gourd (Coccinia grandis (L.) Voigt) and bottle gourd (Lagenaria siceraria (Molina) Standl.) from Chikmagalur, Davanagere and Shivamogga districts of Karnataka in the year 2021. Polymerase chain reaction (PCR) amplification using degenerate primers DengA/DengB and PAL1v/PAR1c confirmed the presence of begomovirus, amplifying expected fragments of ~560 bp and ~1.2–1.3 kb, respectively. Sequencing and BLASTn analysis confirm the presence of Tomato leaf curl New Delhi virus (ToLCNDV). Phylogenetic analysis showed clustering of isolates based on host and region. The ridge gourd (MZ664277), bitter gourd (MZ664278) and ivy gourd (MZ664279) isolates grouped closely, while the bottle gourd (MZ664281) formed a distinct cluster. Identity analyses confirmed 96–100 % sequence similarity with known ToLCNDV strains. The study establishes ToLCNDV as the predominant etiological agent of YMD in these cucurbits in Karnataka.

References

  1. 1. Nath P. Vegetables for the tropical region. New Delhi: Publications and Information Division, ICAR; 1994. p. 194.
  2. 2. Nagarajaiah SB, Prakash J. Chemical composition and bioactive potential of dehydrated peels of Benincasa hispida, Luffa acutangula and Sechium edule. J Herbs Spices Med Plants. 2015;21:193–202. https://doi.org/10.1080/10496475.2014.940437
  3. 3. Yadav NK, Kumar D, Nain J, Beniwal J. Occurrence and severity of cotton leaf curl disease in Haryana. Int J Agric Sci. 2016;8:2450–2.
  4. 4. Nachimuthu S. Development and evaluation of anti-inflammatory ointment using aqueous extract of Coccinia grandis L. Int J Green Pharm. 2018;12(3):589–96. https://doi.org/10.22377/ijgp.v12i03.2025
  5. 5. Sohrab SS, Mandal B, Ali A, Varma A. Chlorotic Curly Stunt: A severe begomovirus disease of bottle gourd in northern India. Indian J Virol. 2010;21:56–63. https://doi.org/10.1007/s13337-010-0002-3
  6. 6. Gopalkrishnan TR. Vegetable crops. Horticulture science series - 4. New Delhi: New India Publishing Agency; 2007.
  7. 7. Zaidi SSEA, Martin DP, Amin I, Farooq M, Mansoor S. Tomato leaf curl New Delhi virus: a widespread bipartite begomovirus in the territory of monopartite begomoviruses. Mol Plant Pathol. 2017;18:901–11. https://doi.org/10.1111/mpp.12481
  8. 8. Rajeshwari. Diagnosis and molecular characterization of Begomovirus infecting Cucurbits [PhD thesis]. Bengaluru: University of Agricultural Sciences; 2016.
  9. 9. Venkataravanappa V, Lakshminarayana CR, Narasimha R, Sujoy S, Shankarappa K, Subbanna, et al. Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India. Arch Biol Sci. 2018;70(2):339–47. https://doi.org/10.2298/ABS170616051V
  10. 10. Nagendran K, Karthika C, Parthasarathy S, Kumar M, Karthikeyan G. Molecular evidence for the occurrence of Tomato leaf curl New Delhi virus on bottle gourd in Tamil Nadu, India. J Veg Sci. 2016;43(1):73–7.
  11. 11. Manjunath SH, Rangaswamy KT, Shweta K. Virus - vector relationships of Yellow Mosaic Virus and whitefly (Bemisia tabaci) in ridge gourd. J Pure Appl Microbiol. 2016;10(4):2883–8. https://doi.org/10.22207/JPAM.10.4.51
  12. 12. Tamilselvan S, Perumal R, Suganthy M, Sankari A, Rajagopal B. Molecular characterization of tomato leaf curl New Delhi virus and its transmission by whitefly Bemisia tabaci in bitter gourd. Pharma Innov J. 2023;12(10):1649–53.
  13. 13. Kushvaha RP, Kushwaha C, Snehi SK. Begomovirus disease of pumpkin crop in India and its management strategies possibility: a review. J Sci Agric. 2024;8:69–78. https://doi.org/10.25081/jsa.2024.v8.9053
  14. 14. Padidam M, Beachy RN, Fauquet CM. Tomato leaf curl geminivirus from India has a bipartite genome and coat protein is not essential for infectivity. J Gen Virol. 1995;76:25–35. https://doi.org/10.1099/0022-1317-76-1-25.
  15. 15. Tiwari AK, Snehi SK, Singh R, Raj SK, Rao GP, Sharm PK. Molecular identification and genetic diversity among six Begomovirus isolates affecting cultivation of cucurbitaceous crops in Uttar Pradesh, India. Arch Phytopathol Pflanzenschutz. 2012;45:62–72. https://doi.org/10.1080/03235400903458803
  16. 16. Patil CV, Ramdas SV, Premch U, Shankarappa KS. Survey, symptomatology, transmission, host range and characterization of begomovirus associated with yellow mosaic disease of ridge gourd in southern India. Virusdisease. 2017;28:146–55. https://doi.org/10.1007/s13337-017-0376-6
  17. 17. Tiwari AK, Sharma PK, Khan SMS, Snehi K, Raj SK, Rao GP. Molecular detection and identification of tomato leaf curl New Delhi virus isolate causing yellow mosaic disease in bitter gourd (Momordica charantia), a medicinally important plant in India. Med Plants. 2010;2:211–21. https://doi.org/10.5958/j.0975-4261.2.2.018
  18. 18. Nagendran K, Satya VK, Mohankumar S, Karthikeyan G. Molecular characterization of a distinct bipartite Begomovirus species infecting ivy gourd (Coccinia grandis L.) in Tamil Nadu, India. Virus Genes. 2014;52:146–51. https://doi.org/10.1007/s11262-015-1278-6
  19. 19. Lodhi MA, Ye GN, Weeden NF, Reisch B. A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Mol Biol Rep. 1994;2:6–13. https://doi.org/10.1007/BF02668658
  20. 20. Maruthi MN, Colvin J, Seal S, Gibson G, Cooper J. Co-adaptation between cassava mosaic geminiviruses and their local vector populations. Virus Res. 2002;86:71–85. https://doi.org/10.1016/s0168-1702(02)00051-5
  21. 21. Deng D, Mcgrath PF, Robinson DJ, Harrison BD. Detection and differentiation of whitefly-transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. Ann Appl Biol. 1994;125:327–36. https://doi.org/10.1111/j.1744-7348.1994.tb04973.x
  22. 22. Rojas MR, Gilbertson RL, Maxwell DP. Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Dis. 1993;77:340–7. https://doi.org/10.1094/PD-77-0340
  23. 23. Fauquet CM, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X, et al. Recommendations for the classification and nomenclature of the DNA-β satellites of begomoviruses. Arch Virol. 2008;153:763–81. https://doi.org/10.1007/s00705-007-0013-6
  24. 24. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30:2725–9. https://doi.org/10.1093/molbev/mst197
  25. 25. Jyothsna P, Haq QMI, Singh P, Sumiya KV, Praveen S, Rawat R, et al. Infection of tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus with betasatellites, results in enhanced level of helper virus components and antagonistic interaction between DNA B and betasatellites. Appl Microbiol Biotechnol. 2013;97:5457–71. https://doi.org/10.1007/s00253-012-4685-9
  26. 26. Seal S, Vandenbosch E, Jeger MJ. Factors influencing begomovirus evolution and their increasing global significance: implications for sustainable control. Crit Rev Plant Sci. 2006;25(1):23–46. https://doi.org/10.1080/07352680500365257
  27. 27. Venkataravanappa V, Lakshminarayana Reddy CN, Salil J, Briddon RW, Krishna Reddy M. Molecular identification and biological characterisation of a begomovirus associated with okra enation leaf curl disease in India. Eur J Plant Pathol. 2015;141:217–35. https://doi.org/10.1007/s10658-014-0463-0

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