A total of 18 lichen species representing 13 genera and 9 families were identified as first report from the Nambor-Doigrung and Garampani Wildlife Sanctuaries of Golaghat district, Assam. This study presents 3 additional lichen taxa as new records from the state, including Herpothallon antillarum, Herpothallon minutum and Ramalina nervulosa. Specimens were examined using standard taxonomic keys to corroborate the morphological and anatomical features for identifying the species. Results highlight the significance of Assam as an important biodiversity hotspot for lichenised fungi, underscoring the considerable potential for further floristic exploration in the region, which may substantially enhance the understanding of its lichen diversity. Furthermore, the study stresses the necessity of implementing appropriate conservation strategies to safeguard lichen communities within this ecologically unique landscape. A comprehensive systematic account of the newly recorded taxa is provided in this report.