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Plant Science Today (PST)

Research Articles

Vol. 13 No. sp1 (2026): Recent Advances in Agriculture

Residue dissipation kinetics, safety evaluation and consumer risk assessment of fipronil in cowpea and the soil

DOI
https://doi.org/10.14719/pst.11861
Submitted
19 September 2025
Published
11-03-2026

Abstract

Fipronil is extensively used for insect pest management in cowpea; however, its residue dynamics under field conditions require a reliable analytical assessment to ensure food safety. In the present study, a sensitive and validated gas chromatography–micro electron capture detector (GC-µECD) method was developed for the determination of fipronil residues in cowpea pods, leaves and soil and its dissipation behaviour was evaluated under field conditions. Field experiments were conducted during the kharif season (2023–24) at ICAR–Indian Institute of Vegetable Research, Varanasi, India, using cowpea (Vigna unguiculata L.) variety Kashi Kanchan in a randomised block design with three replications. Fipronil 5 % SC was applied at the recommended dose (50 g a.i. ha-1) and double the recommended dose using a knapsack sprayer. Samples of pods, leaves and soil were collected at different intervals (0, 1, 2, 5, 7 and 10 days) after the final spray. Residues were extracted and cleaned using a modified QuEChERS method and quantified by GC-µECD. The method showed good linearity over the concentration range of 0.01–0.1 µg mL-1, satisfactory recoveries and a limit of quantification (LOQ) of 0.01 mg kg-1 for all matrices. Fipronil residues dissipated progressively with time, following first-order kinetics and declined below detectable levels within the recommended pre-harvest interval. Therefore, when used at recommended doses, fipronil presents a low risk profile in cowpea cultivation.

References

  1. 1. Gonçalves A, Goufo P, Barros A, Domínguez-Perles R, Trindade H, Rosa EA et al. Cowpea (Vigna unguiculata L. Walp), a renewed multipurpose crop for a more sustainable agri-food system: nutritional advantages and constraints. J Sci Food Agric. 2016;96(9):2941–51. https://doi.org/10.1002/jsfa.7649
  2. 2. Narayan P, Kumar S. Constraints of growth in area production and productivity of pulses in India: an analytical approach to major pulses. Indian J Agric Res. 2015;49(2):114–24.
  3. 3. Muhammad A, Malgwi AM, Nahunnaro H, Adamu RS, Tamo M, Dannon E et al. Effect of sowing dates, intra-row spacings and pesticides on cowpea pod borer, Maruca vitrata (Fab.) populations on cowpea in Katsina, Sudan savanna. Int J Trop Insect Sci. 2022;42:1–9. https://doi.org/10.1007/s42690-022-00671-6
  4. 4. Divekar PA, Halder J, Srivastava K, Sridhar V. Emerging insect pests of vegetable crops under changing climate scenario. Veg Sci. 2024;51:66–76.
  5. 5. Divekar P, Kumar P, Suby SB. Oviposition preference of pink stem borer, Sesamia inferens (Walker) in maize germplasm. J Entomol Zool Stud. 2019;7:1115–9.
  6. 6. Divekar PA, Kumar P, Suby S, Sharma RK, Soumia PS, Pandi G. Screening of maize germplasm for resistance against shoot fly. Indian J Entomol. 2018;80(4):1181–6.
  7. 7. Divekar PA, Mishra A, Singh AK. Plant secondary metabolites for defense against herbivores. In: Mérillon JM, Ramawat KG, editors. Plant specialized metabolites: phytochemistry, ecology and biotechnology. Cham: Springer Nature; 2025. p. 605–16. https://doi.org/10.1007/978-3-031-XXXX-X_28
  8. 8. Divekar PA, Majumder S, Halder J, Kedar SC, Singh V. Sustainable pest management in cabbage using botanicals: characterization, effectiveness and economic appraisal. J Plant Dis Protect. 2024;131(1):113–30. https://doi.org/10.1007/s41348-023-00786-5
  9. 9. Divekar P, Majumder S, Halder J, Dukare A, Singh J. Trilogy: plants-proteases-insects. In: Plant Protection: Present Developments and Future Strategies. 2022. p. 143–157.
  10. 10. Divekar PA, Rani V, Majumder S, Karkute SG, Molla KA, Pandey KK et al. Protease inhibitors: an induced plant defense mechanism against herbivores. J Plant Growth Regul. 2023;42(10):6057–73. https://doi.org/10.1007/s00344-023-10827-6
  11. 11. Sreekanth M, Lakshmi MSM, Rao YK. Efficacy and economics of certain new generation insecticides against legume pod borer, Maruca vitrata on pigeonpea. J Appl Biol Biotechnol. 2015;3(3):7–10. https://doi.org/10.7324/JABB.2015.3302
  12. 12. Kodandaram MH, Divekar PA, Wangi N, Mohite NR, Rai AB. Optimizing sucking pest control in okra: an analysis of flupyradifurone 200 SL effectiveness, phytotoxicity and safety. J Plant Dis Protect. 2024;131(3):757–71. https://doi.org/10.1007/s41348-024-00859-7
  13. 13. Grigolli JFJ, Lourenção ALF, Ávila CJ. Field efficacy of chemical pesticides against Maruca vitrata infesting soybean in Brazil. Am J Plant Sci. 2015;6(4):537–44. https://doi.org/10.4236/ajps.2015.64058
  14. 14. Divekar PA, Patel SK, Manimurugan C, Singh V, Singh J. Spinetoram, a selective novel insecticide against key lepidopteran pests in cabbage ecosystem. Pak J Zool. 2024;56(2):743–51. https://doi.org/10.17582/journal.pjz/20220623070611
  15. 15. Divekar PA, Singh K, Verma CK, Rai AB, Singh B, Yadav S et al. Assessment of bee flora and development of a floral calendar in Eastern Uttar Pradesh, India. Ann Phytomed. 2023;12(1):844–855.
  16. 16. Divekar PA, Mishra A, Kumar R. Bee pollination in vegetables: current status, challenges and prospects. Circ Agric Syst. 2024;4(1):1–12.
  17. 17. Mahalle RM, Taggar GK. Insecticides against Maruca vitrata on pigeonpea. Pestic Res J. 2018;30(2):235–40.
  18. 18. Ministry of Law and Justice. The Insecticides Act, 1968. New Delhi: Government of India; 1968 [updated 2023; cited 2026 Jan 24].
  19. 19. Jiries AG, Al Nasir FM, Beese F. Pesticide and heavy metals residue in wastewater, soil and plants near Al-Lajoun Valley, Jordan. Water Air Soil Pollut. 2002;133:97–107. https://doi.org/10.1023/A:1012981029183
  20. 20. Yu Y, Zhou QX. Adsorption characteristics of methamidophos and glyphosate by two soils. Chemosphere. 2005;58(6):811–6. https://doi.org/10.1016/j.chemosphere.2004.09.033
  21. 21. Li JZ, Wu X, Hu JY. Determination of kresoxim-methyl residues in cucumber and soil by gas chromatography. J Environ Sci Health B. 2006;41(4):427–36. https://doi.org/10.1080/03601230600701667
  22. 22. Majumder S, Mandal S, Majumder B, Paul A, Paul T, Sahana N et al. Determination of acetamiprid and buprofezin residues and dissipation kinetics in paddy matrices. Environ Sci Pollut Res. 2022;29:1401–12. https://doi.org/10.1007/s11356-021-16029-3
  23. 23. Majumder S, Pandey J, Divekar PA, Ali EO, Pandey KK, Behera TK. Dissipation kinetics, food safety evaluation and decontamination of chlorantraniliprole in cowpea. J Environ Sci Health B. 2023;58:1–10. https://doi.org/10.1080/03601234.2023.2216125
  24. 24. Majumder S, Rani AT, Divekar PA, Halder J, Pandey KK, Behera TK. Field bioefficacy and residue dynamics of chlorantraniliprole in okra. Indian J Agric Sci. 2023;93(3):314–317.
  25. 25. European Commission. Analytical quality control and method validation procedures for pesticide residues analysis in food and feed. Brussels: Directorate-General for Health and Food Safety (SANTE); 2021.
  26. 26. Majumder S, Singh P, Pandey J, Abhinay A, Sharma S, Divekar PA et al. Residue dissipation, safety evaluation and decontamination of deltamethrin in tomato. Trends Hortic. 2024;7(1):1–0.
  27. 27. Hingmire S, Oulkar DP, Utture SC, Shabeer TA, Banerjee K. Residue analysis of fipronil and difenoconazole in okra by LC-MS/MS and food safety evaluation. Food Chem. 2015;176:145–51. https://doi.org/10.1016/j.foodchem.2014.12.054
  28. 28. National Sample Survey Office. Household consumption of various goods and services in India. New Delhi: Ministry of Statistics and Programme Implementation, Government of India; 2014 [updated 2025; cited 2026 Jan 24]. Available from: https://www.pib.gov.in/PressReleasePage.aspx?PRID=2111770&reg=3&lang=2
  29. 29. Majumder S, Singh S, Divekar PA, Pandey KK, Behera TK. Residue dissipation kinetics and safety evaluation of hexaconazole in green chilli. Int J Environ Anal Chem. 2022;102:1–13. https://doi.org/10.1080/03067319.2022.2034196

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