Research Articles
Vol. 12 No. sp4 (2025): Recent Advances in Agriculture by Young Minds - III
Formulation of calcium-based nutrition for mitigating bacterial wilt (Ralstonia solanacearum) in tomato
Department of Soil Science and Agricultural Chemistry, University of Agricultural Sciences, Bangalore 560 065, Karnataka, India
Department of Soil Science and Agricultural Chemistry, University of Agricultural Sciences, Bangalore 560 065, Karnataka, India
Department of Plant Pathology, College of Sericulture, Chintamani 563 125, Karnataka, India
College of Sericulture, Chintamani 563 125, Karnataka, India
ICAR - Krishi Vigyan Kendra, Doddaaballapura, University of Agricultural Sciences, Bangalore 560 065, Karnataka, India
Abstract
Bacterial wilt, caused by Ralstonia solanacearum, is a major constraint in tomato (Solanum lycopersicum L.) production, especially in tropical and subtropical regions where favourable soil and climatic conditions support pathogen survival and spread. Conventional management practices have shown limited effectiveness, emphasizing the need for sustainable alternatives. Calcium is an essential nutrient that strengthens cell walls, stabilizes membranes and enhances plant defenses against soil-borne pathogens. This study evaluated calcium-based nutrient formulations for their effectiveness in suppressing bacterial wilt and improving tomato growth and fruit quality as part of a sustainable disease management strategy. In vitro assays using a virulent R. solanacearum isolate from the Eastern Dry Zone of Karnataka showed strong antagonistic activity, producing inhibition zones of 26.00–29.30 mm after 48 hr. The most effective formulation was further tested in a pot experiment arranged in a completely randomized design with six treatments and four replications, using two tomato varieties: Baaho (wilt-susceptible) and Abhinava (wilt-resistant). The calcium-enriched formulation containing calcium, magnesium, sulfur and potassium significantly improved plant growth, yield and fruit quality in wilt-susceptible Baaho while delaying wilt onset up to 60 days after transplanting. At harvest, calcium-based nutrition to plants recorded a height of 84.61 cm, chlorophyll content of 34.52 (SPAD), 42 fruits plant-1, fruit weight of 71.49 g, diameter of 4.14 cm, firmness of 1.44 kg cm-2 and a shelf life of 10.75 days. Abhinava remained resistant and performed better across all treatments. These results demonstrate that calcium-enriched nutrient formulations can effectively suppress bacterial wilt while enhancing yield and fruit quality in tomato.
References
- 1. Jiang G, Zhang Y, Gan G, Li W, Wan W, Jiang Y, et al. Exploring rhizo-microbiome transplants as a tool for protective plant-microbiome manipulation. ISME J. 2022;2:10. https://doi.org/10.1038/s43705-022-00094-8
- 2. Tessema GL, Seid HE. Potato bacterial wilt in Ethiopia: History, current status and future perspectives. PeerJ. 2023;11:e14661. https://doi.org/10.7717/peerj.14661
- 3. Genin S, Denny TP. Pathogenomics of the Ralstonia solanacearum species complex. Annu Rev Phytopathol. 2012;50:67-89. https://doi.org/10.1146/annurev-phyto-081211-173000
- 4. Vanitha SC, Niranjana SR, Mortensen CN, Umesha S. Bacterial wilt of tomato in Karnataka and its management by Pseudomonas fluorescens. Biocontrol. 2009;54(5):685-95. https://doi.org/10.1007/s10526-009-9217-x
- 5. Nion YA, Toyota K. Recent trends in control methods for bacterial wilt diseases caused by Ralstonia solanacearum. Microbes Environ. 2015;ME14144.
- 6. Huber DM, Graham RD. The role of nutrition in crop resistance and tolerance to disease. In: Rengel Z, editor. Mineral Nutrition of Crops: Fundamental Mechanisms and Implications. New York (NY): CRC Press; 1999. p.205-26.
- 7. Agrios GN. Plant Pathology. 5th ed. Amsterdam: Elsevier; 2005.
- 8. Bush DS. Calcium regulation in plant cells and its role in signaling. Annu Rev Plant Physiol Plant Mol Biol. 1995;46:95-122. https://doi.org/10.1146/annurev.pp.46.060195.000523
- 9. Yang T, Poovaiah BW. Hydrogen peroxide homeostasis: Activation of plant catalase by calcium/calmodulin. Proc Natl Acad Sci USA. 2002;99:4097-102. https://doi.org/10.1073/pnas.052564899
- 10. Taylor MD, Locascio SJ. Blossom-end rot: A calcium deficiency. J Plant Nutr. 2004;27:123-39. https://doi.org/10.1081/PLN-120027551
- 11. Yamazaki H, Kikuchi S, Hoshina T, Kimura T. Effect of calcium concentration in nutrient solution on development of bacterial wilt and population of its pathogen Ralstonia solanacearum in grafted tomato seedlings. J Soil Sci Plant Nutr. 2000;46:535-39.
- 12. Ustun N, Altunlu H, Yokaş I, Saygili H. Influence of potassium and calcium levels on severity of tomato pith necrosis and yield of greenhouse tomatoes. Acta Hortic. 2009;808:347-50. https://doi.org/10.17660/ActaHortic.2009.808.56
- 13. Akhtar ME, Khan MZ, Rashis MT, Ahsan Z, Ahmad S. Effect of potash application on yield and quality of tomato (Lycopersicon esculentum Mill.). Pak J Bot. 2010;42(3):1695-702.
- 14. Ahmed N, Zhang B, Bozdar B, Chachar S, Rai M, Li J, et al. The power of magnesium: Unlocking the potential for increased yield, quality and stress tolerance of horticultural crops. Front Plant Sci. 2023;14:1285512. https://doi.org/10.3389/fpls.2023.1285512
- 15. Tian G, Qin H, Liu C, Xing Y, Feng Z, Xu X, et al. Magnesium improved fruit quality by regulating photosynthetic nitrogen use efficiency, carbon-nitrogen metabolism and anthocyanin biosynthesis in 'Red Fuji' apple. Front Plant Sci. 2023;14:1136179. https://doi.org/10.3389/fpls.2023.1136179
- 16. Kunstler A, Gullner G, Ádám AL, Kolozsváriné Nagy J, Király L. The versatile roles of sulfur-containing biomolecules in plant defense. Plants. 2020;9(12):1705. https://doi.org/10.3390/plants9121705
- 17. Tripathi R, Tewari R, Singh KP, Keswani C, Minkina T, Srivastava AK, et al. Plant mineral nutrition and disease resistance: A significant linkage for sustainable crop protection. Front Plant Sci. 2022;13:883970. https://doi.org/10.3389/fpls.2022.883970
- 18. Bayındır Ü, Küçükyumuk Z. The effects of potassium on plant nutrient concentration, plant development and Rhizoctonia rot in pepper. Horticulturae. 2025;11(5):516. https://doi.org/10.3390/horticulturae11050516
- 19. Kelman A. The relationship of pathogenicity of Pseudomonas solanacearum to colony appearance on a tetrazolium medium. Phytopathology. 1954;44:693-5. https://doi.org/10.1094/Phyto-44-693
- 20. Vincent JM, Humphrey B. Gram staining. In: Norris JR, editor. Methods in Microbiology. Vol.3B. London: Academic Press; 1970. p.143-5.
- 21. Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966;45(4):493-96. https://doi.org/10.1093/ajcp/45.4_ts.493
- 22. Cappuccino JG, Sherman N. Microbiology: A Laboratory Manual. 7th ed. San Francisco (CA): Pearson Education; 2005.
- 23. Jackson ML. Soil Chemical Analysis. New Delhi: Prentice Hall of India; 1973.
- 24. Ziedan EHE. Studies on Fusarium wilt diseases of sesame (Sesamum indicum) in A.R.E. [MSc thesis]. Cairo: Ain Shams Univ; 1993.
- 25. Sheoran OP, Tonk DS, Kaushik LS, Hasija RC, Pannu RS. Statistical software package for agricultural research workers. Hisar (India): CCS HAU; 1998.
- 26. He K, Yang SY, Li H, Wang H, Li ZL. Effects of calcium carbonate on the survival of Ralstonia solanacearum in soil and control of tobacco bacterial wilt. Eur J Plant Pathol. 2014;140(4):665-75. https://doi.org/10.1007/s10658-014-0496-4
- 27. Ferreira MV, Naranjo E, Denis N, Cobine P, De La Fuente L, Siri MI. Calcium modulation of bacterial wilt disease on potato. Appl Environ Microbiol. 2024;90(6):e00242-24. https://doi.org/10.1128/aem.00242-24
- 28. Xu W, Huang W. Calcium-dependent protein kinases in phytohormone signaling pathways. Int J Mol Sci. 2017;18(11):2436. https://doi.org/10.3390/ijms18112436
- 29. Yuan P, Tanaka K, Poovaiah BW. Calcium/calmodulin-mediated defense signaling: AtSR1/CAMTA3-mediated signaling in plant immunity. Front Plant Sci. 2022;12:795353. https://doi.org/10.3389/fpls.2021.795353
- 30. Bleau JR, Spoel SH. Selective redox signaling shapes plant-pathogen interactions. Plant Physiol. 2021;186(1):53-65. https://doi.org/10.1093/plphys/kiaa088
- 31. Aldon D, Mbengue M, Mazars C, Galaud JP. Calcium signalling in plant biotic interactions. Int J Mol Sci. 2018;19(3):665. https://doi.org/10.3390/ijms19030665
- 32. Negi NP, Prakash G, Narwal P, Panwar R, Kumar D, Chaudhry B, et al. The calcium connection: Exploring calcium signaling in plant-microbe interactions. Front Plant Sci. 2023;14:1248648. https://doi.org/10.3389/fpls.2023.1248648
- 33. Wang Y, Dai X, Xu G, Dai Z, Chen P, Zhang T, et al. The Ca2+-CaM signaling pathway mediates potassium uptake under low-K+ stress in tobacco roots. Front Plant Sci. 2021;12:658609. https://doi.org/10.3389/fpls.2021.658609
- 34. Rosyidah A, Murwani I, Siswadi B. Effect of potassium fertilizer on resistance and growth of tomato to bacterial wilt caused by Ralstonia solanacearum. Int J Environ Agric Biotechnol. 2017;2:2265-69. https://doi.org/10.22161/ijeab/2.4.89
- 35. Koesrini W. Effect of ameliorant application on plant growth and yield of three snap bean varieties on acid sulphate soil. J Agron Indones. 2009;37:34-39.
- 36. Oliveira IPD, Asher CJ, Edwards DG, Santos RSM. Magnesium sulphate and development of common bean cultivated in an Ultisol. Sci Agric. 2000;57:153-57. https://doi.org/10.1590/S0103-90162000000100025
- 37. Turan MA, Taban S, Katkat AV, Kucukyumuk Z. Evaluation of elemental sulfur and gypsum effect on soil pH and EC. J Food Agric Environ. 2013;11:572-75.
- 38. Theresa K, Shanmugasundaram R, Kennedy JS. Effect of varied NPK levels on soil reaction and electrical conductivity. Int J Chem Stud. 2020;8:2632-36. https://doi.org/10.22271/chemi.2020.v8.i1an.8669
- 39. Sharma SP, Subehia SK, Sharma PK. Research bulletin on long-term effects of chemical fertilizers on soil quality, crop productivity and sustainability. Palampur: Dept Soil Sci, CSK HPKV; 2002.
- 40. Adekiya AO, Alori ET, Aboyeji CM, Dunsin O, Adegbite KA, Aremu CO, et al. MgO fertilizer effects on soil properties and turmeric quality in a tropical Alfisol. Sci World J. 2019;2019:8140276. https://doi.org/10.1155/2019/8140276
- 41. Liu Y, Zhang Z, Wang X, Wu R, Ding S, Wang S, et al. Gypsum application increases microbial activity and organic carbon mineralization in saline paddy soils. Appl Soil Ecol. 2025;208:106004. https://doi.org/10.1016/j.apsoil.2025.106004
- 42. Gokila B, Manimaran G, Jayanthi D, Sivakumar K, Sridevi G, Thenmozhi S, et al. Long-term fertilization effects on sulphur distribution and SOC in an Inceptisol. Sci Rep. 2024;14:9758. https://doi.org/10.1038/s41598-024-60357-3
- 43. Kaewu N. Effects of agricultural potassium fertilizer application on soil carbon cycle. Acad J Sci Technol. 2024;11(2):122-25. https://doi.org/10.54097/fy0zmz82
- 44. Mir L, Sheikh YA, Kumar R, Shahnaz E, Ahmed S, Butt IA, et al. Effects of calcium nitrate application on soil properties in apple plantations. J Sci Res Rep. 2025;31:265-73. https://doi.org/10.9734/jsrr/2025/v31i22845
- 45. Syamsiyah J, Hartati S, Rahmanisa L, Maro'ah S, Herdiansyah G. Effect of balanced N, P, K, Ca, Mg fertilizer on soil and leaf nutrients and corn yield (Zea mays L.). J Aridland Agric. 2024;10:58-64. https://doi.org/10.25081/jaa.2024.v10.8646
- 46. Talukdar L, Dutta S, Dutta P, Hussain J. Nitrogen and sulphur interaction on nutrient use efficiency in field crops. J Pharm Innov. 2022;11:1372-76.
- 47. Tadesse T, Dechassa N, Bayu W, Gebeyehu S. Effects of farmyard manure and inorganic fertilizer on soil properties and nutrient balance in rain-fed rice. Am J Plant Sci. 2013;4:309-16. https://doi.org/10.4236/ajps.2013.42041
- 48. Hemmaty S, Dilmaghani MR, Naseri L. Effects of sulfur application on soil pH and uptake of P, Fe and Zn in apple trees. J Plant Physiol Breed. 2012;2(1):1-10.
- 49. Rakotoson T, Tsujimoto Y. Effect of farmyard manure on P availability to rice in low C and low pH paddy soils. Plant Prod Sci. 2020;23:314-21. https://doi.org/10.1080/1343943X.2020.1740601
- 50. Sindhu GP, Reddy KS, Gunasekar J, Vamshi M. Effect of MOP and SOP on growth of green gram (Vigna radiata L.). Int J Curr Microbiol Appl Sci. 2019;8:2577-92. https://doi.org/10.20546/ijcmas.2019.802.300
- 51. Bossolani JW, Crusciol CAC, Garcia A, Moretti LG, Portugal JR, Rodrigues VA, et al. Long-term lime and phosphogypsum amended soils alleviate drought by improving soil fertility and root growth. Front Plant Sci. 2021;12:650296. https://doi.org/10.3389/fpls.2021.650296
- 52. Zhang Q, Tang D, Yang X, Geng S, He Y, Chen Y, et al. Plant availability of magnesium in tea plantation soils. Front Plant Sci. 2021;12:641501. https://doi.org/10.3389/fpls.2021.641501
- 53. Radwan S, Abouhussien EHE, Tantawy M, Hassan S. Effect of elemental sulfur and gypsum on growth and nutrient content of barley in saline soils. Menoufia J Soil Sci. 2024;9:17-29. https://doi.org/10.21608/mjss.2024.272076.1024
- 54. Mondal S, Ghosh GK, Mandal J. Effect of graded sulphur as magnesium sulphate on yield and quality of onion in red soils. Int J Curr Microbiol Appl Sci. 2020;9(1):1-9. https://doi.org/10.20546/ijcmas.2020.904.335
- 55. Birgin O, Akhoundnejad Y, Dasgan HY. Effect of foliar calcium application in tomato under drought stress. Appl Ecol Environ Res. 2021;19(4):2971-82. https://doi.org/10.15666/aeer/1904_29712982
- 56. Chauhan S, Deependra Y, Kumar S, Kumar R, Kumar A. Effect of calcium on growth and yield of tomato (Solanum lycopersicum L.). Biol Forum Int J. 2023;15(2):1162-6.
- 57. Alrashidi AA, Alhaithloul HAS, Soliman MH, Attia MS, Elsayed SM, Sadek AM, et al. Role of calcium and magnesium on physiological and anatomical responses in tomato plants. Not Bot Horti Agrobo. 2022;50(1):12614. https://doi.org/10.15835/nbha50112614
- 58. Narayan OP, Kumar P, Yadav B, Dua M, Johri AK. Sulfur nutrition and its role in plant growth and development. Plant Signal Behav. 2023;18:2030082. https://doi.org/10.1080/15592324.2022.2030082
- 59. Ddamulira G, Idd R, Namazzi S, Kalali F, Mundingotto J, Maphosa M. Nitrogen and potassium fertilizers increase cherry tomato height and yield. J Agric Sci. 2019;11(10):207. https://doi.org/10.5539/jas.v11n13p48
- 60. Naciri R, Lahrir M, Benadis C, Chtouki M, Oukarroum A. Interactive effect of potassium and cadmium on growth and chlorophyll fluorescence in tomato. Sci Rep. 2021;11:5384. https://doi.org/10.1038/s41598-021-84990-4
- 61. Tongali NC, Ashoka KR, Ananthakumar MA, Kumar NK. Effect of calcium sources and levels on growth and yield of tomato in Alfisols. Plant Arch. 2024;24. https://doi.org/10.51470/PLANTARCHIVES.2024.v24.no.2.066
- 62. Santhosh SS, Chitdeshwari T, Jegadeeswari D, Kavitha C. Effect of calcium levels and sources on post-harvest physiology of hybrid tomatoes. J Appl Nat Sci. 2021;13(4):1357-64. https://doi.org/10.31018/jans.v13i4.3058
- 63. Kasinath BL, Ganeshamurthy AN, Nagegowda NS. Effect of magnesium on growth, dry matter and yield in tomato (Lycopersicon esculentum L.). J Hortic Sci. 2015;10:190-3. https://doi.org/10.24154/jhs.v10i2.128
- 64. Rashid M. Effect of sulphur and boron fertilizer on growth and yield of Bari tomato-2 (Lycopersicon esculentum). [Thesis]. Sher-e-Bangla Agricultural University, Dhaka. 2021.
- 65. Ahmad N, Sarfraz M, Farooq U, Arfan-ul-Haq M, Mushtaq MZ, Ali MA. Effect of potassium and its time of application on yield and quality of tomato. Int J Sci Res Publ. 2015;5(3):1-4.
- 66. Polwaththa KDM, Amarasinghe AY. Optimizing calcium application strategies to enhance fruit quality in tomato (Lycopersicon esculentum L.). Int J Sci Res Arch. 2024;13(02):596–602. https://doi.org/10.30574/ijsra.2024.13.2.2188
- 67. Tian G, Qin H, Liu C, Xing Y, Feng Z, Xu X, et al. Magnesium improved fruit quality by regulating nitrogen use efficiency in apple. Front Plant Sci. 2023;14:1136179. https://doi.org/10.3389/fpls.2023.1136179
- 68. Timileyin OA, Olajide BT, Temitayo IA, Faith OO, Arem WT, Nwafor IP. Effect of potassium fertilizer rate on growth, fruit yield and nutritional quality of tomato varieties. Int J Res Publ Rev. 2025;6(4):14752-772. https://doi.org/10.55248/gengpi.6.0425.16148
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