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Plant Science Today (PST)

Research Articles

Early Access

Comprehensive phytochemical profiling of Curcuma caesia Roxb. rhizome using HRLC-MS and GC-MS

DOI
https://doi.org/10.14719/pst.12673
Submitted
10 November 2025
Published
14-04-2026

Abstract

Curcuma caesia Roxb., also called black turmeric, belonging to the Zingiberaceae family, is a rhizomatous herb and is widely known for its medicinal importance, such as for its antibacterial, anti-inflammatory and antioxidant properties. Despite its medicinal properties, the characterisation of secondary metabolites remains scarce. Total phenolic and flavonoid content were evaluated to validate the therapeutic value of the plant. This research also focuses on the profiling of the methanolic extract of C. caesia rhizomes using high-resolution liquid chromatography-mass spectrometry (HR LC-MS) and gas chromatography-mass spectrometry (GC-MS). Through the metabolite profiling, the study revealed more than 50 phytochemical compounds, including phenols, flavonoids, terpenoids, alkaloids and fatty acids. Extracts also showed elevated peak intensities, like curcumene, valine, cinnamic acid and (+)-ar-turmerone. The medicinal importance of C. caesia is highlighted by its unique phytochemicals, found via GC-MS and LC-MS analysis, which are also present in other Curcuma species as reported in the literature. These results bridge the gap between previous research studies and contemporary analytical science, suggesting the inclusion of this plant in cosmetic, nutraceutical and pharmaceutical advancements for high-value product development.

References

  1. 1. Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis. Springer Science and Business Media; 1998. https://doi.org/10.1007/978-94-009-5921-7
  2. 2. Ballester P, Cerdá B, Arcusa R, García-Muñoz AM, Marhuenda J, Zafrilla P. Antioxidant activity in extracts from Zingiberaceae family: cardamom, turmeric and ginger. Molecules. 2023;28(10):4024. https://doi.org/10.3390/molecules28104024
  3. 3. Razak AM, Tan JK, Mohd Said M, Makpol S. Modulating effects of Zingiberaceae phenolic compounds on neurotrophic factors and their potential as neuroprotectants in brain disorders and age-associated neurodegenerative disorders: A review. Nutrients. 2023;15(11):2564. https://doi.org/10.3390/nu15112564
  4. 4. Xu Y, Chen G, Guo Q. Advances in mass spectrometry for natural products research: developments and applications. Anal Chim Acta. 2017;1033:1–13. https://doi.org/10.1016/j.aca.2017.04.004
  5. 5. Mohan S, Nair P, Rao S. Phytochemical constituents and pharmacological activities of Curcuma species. J Herbal Med. 2019;17:100–12.
  6. 6. Punia H, Yadav P, Singh R. Advanced techniques in phytochemical analysis of medicinal plants. Curr Anal Chem. 2020;16(1):2–35.
  7. 7. Ravindra N, Verma S, Jain A. Ethnomedicinal and therapeutic potential of Curcuma caesia Roxb.: A review. J Ethnopharmacol. 2018;224:77–89. https://doi.org/10.1016/j.jep.2018.05.022
  8. 8. Daimary M, Islary P, Daimari R. Phytochemical, proximate analysis and antioxidant activity of the rhizome of Alpinia nigra (Gaertn.) BL Burtt (Zingiberaceae) in Tamulpur district, Assam. Plant Sci. Today. 2024;11(3):172–82. https://doi.org/10.14719/pst.2929
  9. 9. Singh PK, Singh J, Medhi T, Kumar A. Phytochemical screening, quantification, FT-IR analysis and in silico characterization of potential bio-active compounds identified in HR-LC/MS analysis of the polyherbal formulation from Northeast India. ACS Omega. 2022;7(37):33067–78. https://doi.org/10.1021/acsomega.2c03117
  10. 10. Cushnie TPT, Cushnie B, Lamb AJ. Alkaloids: An overview of their antibacterial, antibiotic-enhancing and antivirulence activities. Int J Antimicrob Agents. 2014;44(5):377–86. https://doi.org/10.1016/j.ijantimicag.2014.06.001
  11. 11. Sonibare MA, Isola AO, Akinmurele OJ. Pharmacognostic standardisation of the leaves of Costus afer Ker Gawl. (Zingiberaceae) and Palisota hirsuta (Thunb.) K Schum. (Commelinaceae). Future J Pharm Sci. 2023;9(1):19. https://doi.org/10.1186/s43094-023-00469-1
  12. 12. Hucklenbroich J, Klein R, Neumaier B, Graf R, Fink GR, Schroeter M, et al. Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo. Stem Cell Res Ther. 2014;5(4):100. https://doi.org/10.1186/scrt500
  13. 13. Mohan S, Nair V, Rao LJM. Comparative analysis of curcuminoids in different Curcuma species: implications for quality assessment and standardization. Food Chem. 2019;299:125141. https://doi.org/10.1016/j.foodchem.2019.125141
  14. 14. Punia S, Sandhu KS, Siroha AK, Dhull SB. Omega 3-metabolism, absorption, bioavailability and health benefits: A review. PharmaNutrition. 2020;10:100162. https://doi.org/10.1016/j.phanu.2019.100162
  15. 15. Ravindra P, Bhowmik D, Duraivel S, Harish G. Traditional and medicinal uses of Curcuma caesia Roxb. J Med Plants Res. 2018;12(15):190–5.
  16. 16. Shimomura Y, Murakami T, Nakai N, Nagasaki M, Harris RA. Exercise promotes BCAA catabolism: effects of BCAA supplementation on skeletal muscle during exercise. J Nutr. 2004;134(6):1583S–87S. https://doi.org/10.1093/jn/134.6.1583S
  17. 17. Singh A, Pandey R, Gupta S. Conservation genetics and sustainable utilization of medicinal plants: A case study of Curcuma species. Genet Resour Crop Evol. 2021;68(4):1421–35. https://doi.org/10.1007/s10722-020-01089-x
  18. 18. Sova M. Antioxidant and antimicrobial activities of cinnamic acid derivatives. Mini Rev Med Chem. 2012;12(8):749–67. https://doi.org/10.2174/138955712801264792
  19. 19. Suman S, Dahiya B. Phytochemical analysis and therapeutic potential of Curcuma caesia Roxb.: A comprehensive review. Asian J Pharm Clin Res. 2018;11(8):45–52.
  20. 20. Wagner H, Ulrich-Merzenich G. Synergy research: Approaching a new generation of phytopharmaceuticals. Phytomedicine. 2009;16(2-3):97–110. https://doi.org/10.1016/j.phymed.2008.12.018
  21. 21. Wolfender JL, Marti G, Thomas A, Bertrand S. Current approaches and challenges for the metabolite profiling of complex natural extracts. J Chromatogr A. 2015;1382:136–64. https://doi.org/10.1016/j.chroma.2014.10.091
  22. 22. Weiß HF. Metabolomic profiling of some medicinal plants. In: CRC Press eBooks. 2023. p. 181–209. https://doi.org/10.1201/9781003179139-10
  23. 23. Fernández-Marín R, Fernandes SC, Andrés MA, Labidi J. Microwave-assisted extraction of Curcuma longa L. oil: optimization, chemical structure and composition, antioxidant activity and comparison with conventional soxhlet extraction. Molecules. 2021;26(6):1516. https://doi.org/10.3390/molecules26061516
  24. 24. Benya A, Mohanty S, Hota S, Das AP, Rath CC, Achary KG, et al. Endangered Curcuma caesia Roxb.: qualitative and quantitative analysis for identification of industrially important elite genotypes. Ind Crops Prod. 2023;195:116363. https://doi.org/10.1016/j.indcrop.2023.116363
  25. 25. Nisar T, Iqbal M, Raza A, Safdar M, Iftikhar F, Waheed M. Estimation of total phenolics and free radical scavenging of turmeric (Curcuma longa). Environ Sci. 2015;15(7):1272–77.
  26. 26. Alafiatayo Akinola A, Ahmad S, Maziah M. Total antioxidant capacity, total phenolic compounds and the effects of solvent concentration on flavonoid content in Curcuma longa and Curcuma xanthorhhiza rhizomes. J Med Aromat Plants. 2014;3(156):2167–412. https://doi.org/10.4172/2167-0412.1000156
  27. 27. Yang FQ, Li SP, Zhao J, Lao SC, Wang YT. Optimization of GC-MS conditions based on resolution and stability of analytes for simultaneous determination of nine sesquiterpenoids in three species of Curcuma rhizomes. J Pharm Biomed Anal. 2007;43(1):73–82. https://doi.org/10.1016/j.jpba.2006.06.014
  28. 28. Herebian D, Choi JH, Abd El-Aty AM, Shim JH, Spiteller M. Metabolite analysis in Curcuma domestica using various GC-MS and LC-MS separation and detection techniques. Biomed Chromatogr. 2009;23(9):951–65. https://doi.org/10.1002/bmc.1207
  29. 29. Zhang L, Fang Y, Cheng X, Lian Y, Xu H. Curcumin derivative and its nanoformulation: recent progress in chemotherapy. Pharmacol Res. 2019;147:104341. https://doi.org/10.1016/j.phrs.2019.104341
  30. 30. Musdalipah M, Tee SA, Karmilah K, Sahidin S, Fristiohady A, Yodha AWM. Total phenolic and flavonoid content, antioxidant and toxicity test with BSLT of Meistera chinensis fruit fraction from Southeast Sulawesi. Borneo J Pharm. 2021;4(1):6–15. https://doi.org/10.33084/bjop.v4i1.1686
  31. 31. Moise G, Jîjie AR, Moacă EA, Predescu IA, Dehelean CA, Hegheș A, et al. Plants impact on the human brain-exploring the neuroprotective and neurotoxic potential of plants. Pharmaceuticals. 2024;17(10):1339. https://doi.org/10.3390/ph17101339
  32. 32. Mohd Sairazi NS, Sirajudeen KNS. Natural products and their bioactive compounds: neuroprotective potentials against neurodegenerative diseases. Evid Based Complement Alternat Med. 2020;2020(1):6565396. https://doi.org/10.1155/2020/6565396
  33. 33. Akinola AA, Ahmad S, Maziah M. Total anti-oxidant capacity, flavonoid, phenolic acid and polyphenol content in ten selected species of Zingiberaceae rhizomes. Afr J Tradit Complement Altern Med. 2014;11(3):7–13. https://doi.org/10.4314/ajtcam.v11i3.2
  34. 34. Mokhtar N, Nordin MFM, Morad NA. Total phenolic content, total flavonoid content and radical scavenging activity from Zingiber zerumbet rhizome using subcritical water extraction. Int J Eng. 2018;31(8):1421–9. https://doi.org/10.5829/ije.2018.31.08b.34
  35. 35. Williams CA, Harborne JB. The leaf flavonoids of the Zingiberales. Biochem Syst Ecol. 1977;5(3):221–9. https://doi.org/10.1016/0305-1978(77)90008-4
  36. 36. Mutakin, Saptarini NM, Amalia R, Sumiwi SA, Megantara S, Saputri FA, et al. Molecular docking simulation of phenolics towards tyrosinase, phenolic content and radical scavenging activity of some Zingiberaceae plant extracts. Cosmetics. 2023;10(6):149. https://doi.org/10.3390/cosmetics10060149
  37. 37. Ghasemzadeh A, Jaafar HZ, Rahmat A, Wahab PEM, Halim MRA. Effect of different light intensities on total phenolics and flavonoids synthesis and anti-oxidant activities in young ginger varieties (Zingiber officinale Roscoe). Int J Mol Sci. 2010;11(10):3885–97. https://doi.org/10.3390/ijms11103885
  38. 38. Wardana AP, Aminah NS, Kristanti AN, Fahmi MZ, Abdjan MI, Sucipto TH. Antioxidant, anti-inflammatory, antiviral and anticancer potentials of Zingiberaceae species used as herbal medicine in Indonesia. Trop J Nat Prod Res. 2024;8(9). https://doi.org/10.26538/tjnpr/v8i9.22
  39. 39. Supartiningsih S, Mairani F, Putri AD, Panggabean IE, Situmorang ISO, Erni E, et al. Isolation of secondary metabolites of Zingiberaceae rhizomes by spectrofotometry and chromatography: A review. NSMRJ Nusant Sci Med Res J. 2025;3(02):39–45.
  40. 40. Muflihah YM, Gollavelli G, Ling YC. Correlation study of antioxidant activity with phenolic and flavonoid compounds in 12 Indonesian indigenous herbs. Antioxidants. 2021;10(10):1530. https://doi.org/10.3390/antiox10101530
  41. 41. Tunnisa F, Faridah DN, Afriyanti A, Rosalina D, Syabana MA, Darmawan N, et al. Antioxidant and antidiabetic compounds identification in several Indonesian underutilized Zingiberaceae spices using SPME-GC/MS-based volatilomics and in silico methods. Food Chem X. 2022;14:100285. https://doi.org/10.1016/j.fochx.2022.100285
  42. 42. Mendez NP, Elbit MGD, Villafranca-Tuba AR, Lagunday NE, Mendez RA. Antioxidant activity, phenolic content and flavonoid content of genus Hedychium (Hedychieae, Zingiberaceae) in the Philippines. Philipp J Sci. 2023;152. https://doi.org/10.56899/152.6A.11

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