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Plant Science Today (PST)

Research Articles

Vol. 13 No. sp1 (2026): Recent Advances in Agriculture

Unveiling the therapeutic and insecticidal potential of dangling coneflower: A comprehensive study

DOI
https://doi.org/10.14719/pst.12978
Submitted
28 November 2025
Published
13-02-2026

Abstract

Newly described plant species represent valuable yet underexplored sources of bioactive compounds. Strobilanthes jomyi (Dangling coneflower) is an endemic species of the Western Ghats, Kerala, India. The current study sought to assess the anthelmintic, insecticidal, antimicrobial and cytotoxic properties of a methanolic extract of S. jomyi leaves, stem and root. Extracts were prepared using Soxhlet extraction and screened using standard in vitro bioassays. All vegetative parts exhibited significant anthelmintic activity, with the leaf extract showing the shortest death time (91.66 ± 2.08 min), followed by root (115 ± 5 min) and stem (115 ± 5 min) extracts. Insecticidal activity was observed only in the leaf extract (100 % at 25 mg/mL). Anti-microbial activity of all vegetative parts of S. jomyi showed mild activity at higher concentrations (10 mg/mL). Cytotoxic evaluation against HeLa, MCF-7 and HT-29 cell lines demonstrated dose-dependent activity, with IC₅₀ values within the range of 10–100 µg/mL. GC-MS results proved the presence of bioactive compounds like n-hexadecenoic acid, stigmasterol, phytol, neophytadiene, tris(2,4-di-tert-butylphenyl) phosphate, Squalene, 1-Eicosanol, lupeol and gamma-sitosterol, which in turn could be responsible for the anthelmintic, insecticidal, anti-microbial and cytotoxic activity of S. jomyi. Taken together, the findings suggest that S. jomyi represents a promising source of bioactive compounds with potential pharmacological and agricultural relevance, warranting further isolation and mechanistic studies.

References

  1. 1. Oyebode O, Kandala NB, Chilton PJ, Lilford RJ. Use of traditional medicine in middle-income countries: a WHO-SAGE study. Health Policy Plan. 2016;31(8):984–91. https://doi.org/10.1093/heapol/czw022
  2. 2. Katiyar C, Gupta A, Kanjilal S, Katiyar S. Drug discovery from plant sources: an integrated approach. Ayu. 2012;33(1):10. https://doi.org/10.4103/0974-8520.100295
  3. 3. Shelar DB, Shirote PJ. Natural product in drug discovery: back to future. Biomed Pharmacol J. 2011;4(1):141. https://doi.org/10.13005/bpj/272
  4. 4. Athanasiadou S, Githiori J, Kyriazakis I. Medicinal plants for helminth parasite control: facts and fiction. Animal. 2007;1(9):1392–400. https://doi.org/10.1017/S1751731107000730
  5. 5. Okwute SK. Plants as potential sources of pesticidal agents: a review. Pesticides. 2012;10:208–32.
  6. 6. Mathur G, Nain S, Sharma PK. Cancer: an overview. Acad J Cancer Res. 2015;8(1):1-9.
  7. 7. Benelli G, Pavela R, Drenaggi E, Desneux N, Maggi F. Phytol, (E)-nerolidol and spathulenol from Stevia rebaudiana leaf essential oil as botanical insecticides against Metopolophium dirhodum. Ind Crops Prod. 2020;155:112844. https://doi.org/10.1016/j.indcrop.2020.112844
  8. 8. Punnakot B, Josekutty EJ, Damodaran R, Wood JR. Strobilanthes jomyi, a new species from South India. Phytotaxa. 2017;332(1):75–80.
  9. 9. Zhu XL, Xu Y, Sun DJ, Li H, Chen LX. The genus Strobilanthes: phytochemistry and pharmacology. TMR Mod Herb Med. 2022;5(3):15. https://doi.org/10.53388/mhm2022B0701001
  10. 10. Sundaram V, Sadhasivam S, Chandrasekaran S, Nanjian R, Pandian A. Strobilanthes heyneanus root extract as a source of antioxidant and antimicrobial activity. Future J Pharm Sci. 2021;7(1):1–2. https://doi.org/10.1186/s43094-021-00242-2
  11. 11. Lim V, Seng Yap C, Wen Chong H, Saleem Abdul Shukkoor M, Priya M. Antimicrobial evaluation and GC-MS analysis of Strobilanthes crispus ethanolic leaf extract. Euro J Med Plants. 2015;10(4):1-8.
  12. 12. Prasad S, Chaurasiya A, Singh RP. A review on anthelmintic synthetic drugs and medicinal plants. SGVU J Pharm Res Educ. 2018;3(2):330–7.
  13. 13. Gangaram S, Naidoo Y, Dewir YH, El-Hendawy S. Phytochemicals and biological activities of Barleria. Plants. 2021;11(1):82. https://doi.org/10.3390/plants11010082
  14. 14. Kshirsagar AD, Ingale KG, Vyawahare NS, Thorve VS. Hygrophila spinosa: a comprehensive review. Pharmacogn Rev. 2010;4(8):167. https://doi.org/10.4103/0973-7847.70912
  15. 15. Govindarajan M. Evaluation of Andrographis paniculata extracts against Culex quinquefasciatus and Aedes aegypti. Asian Pac J Trop Med. 2011;4(3):176–81. https://doi.org/10.1016/S1995-7645(11)60064-3
  16. 16. Sharma A, Patel S. Preliminary phytochemical screening and quantitative analysis of secondary metabolites of Mentha arvensis and Azadirachta indica. Int J Adv Res Dev. 2018;3(1):114–8.
  17. 17. Redfern J, Kinninmonth M, Burdass D, Verran J. Soxhlet ethanol extraction to test antimicrobial properties of plant material. J Microbiol Biol Educ. 2014;15(1):45–6. https://doi.org/10.1128/jmbe.v15i1.656
  18. 18. Irawan C, Sulistiawaty L, Sukiman M. GC-MS analysis and antioxidant activity of Archidendron bubalinum husk. Pharmacogn J. 2018;10(1). https://doi.org/10.5530/pj.2018.1.17
  19. 19. Bhinge SD, Desai P, Magdum CS. In vitro anthelmintic activity of Adhatoda vasica leaf extracts. Dhaka Univ J Pharm Sci. 2015;14(2):153–5. https://doi.org/10.3329/dujps.v14i2.28504
  20. 20. Yenisetti SC, Hegde SN. Size-related mating success in Phorticella striata. Zool Stud. 2003;42(1):203–10.
  21. 21. Khan S, Taning CNT, Bonneure E, Mangelinckx S, Smagghe G, Shah MM. Insecticidal activity of plant-derived extracts against pest insects. Phytoparasitica. 2017;45(1):113–24. https://doi.org/10.1007/s12600-017-0569-y
  22. 22. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluation of antimicrobial activity. J Pharm Anal. 2016;6(2):71–9. https://doi.org/10.1016/j.jpha.2015.11.005
  23. 23. Deniz UG, Gunes H, Gunes F, Mammadov R. Cytotoxic activities of medicinal plants on cancer cell lines. Turk J Pharm Sci. 2017;14(3):222. https://doi.org/10.4274/tjps.80299
  24. 24. Aparna V, Dileep KV, Mandal PK, Karthe P, Sadasivan C, Haridas M. Anti-inflammatory property of n-hexadecanoic acid. Chem Biol Drug Des. 2012;80(3):434–9. https://doi.org/10.1111/j.1747-0285.2012.01418.x
  25. 25. Kaur N, Chaudhary J, Jain A, Kishore L. Stigmasterol: a comprehensive review. Int J Pharm Sci. 2011;2(9):2259.
  26. 26. de Moraes J, de Oliveira RN, Costa JP, Junior AL, de Sousa DP, Freitas RM, et al. Phytol as a drug against Schistosoma mansoni. PLoS Negl Trop Dis. 2014;8(1):e2617. https://doi.org/10.1371/journal.pntd.0002617
  27. 27. Pratama OA, Tunjung WA, Sutikno S, Daryono BS. Bioactive compounds of Cucumis melo leaf extract infected by downy mildew. Biodiversitas. 2019;20(11). https://doi.org/10.13057/biodiv/d201143
  28. 28. Vinuchakkaravarthy T, Sangeetha CK, Velmurugan D. Tris(2,4-di-tert-butylphenyl) phosphate. Acta Crystallogr E Struct Rep Online. 2010;66(9):o2207–8. https://doi.org/10.1107/S1600536810029673
  29. 29. Huang ZR, Lin YK, Fang JY. Biological and pharmacological activities of squalene. Molecules. 2009;14(1):540–54. https://doi.org/10.3390/molecules14010540
  30. 30. Chatterjee S, Karmakar A, Azmi SA, Barik A. Antibacterial activity of long-chain alcohols from Solena amplexicaulis. Proc Zool Soc. 2018;71:313–9. https://doi.org/10.1007/s12595-017-0208-0
  31. 31. Patil P, Soujanya B, Kiran KA. A review on lupeol from horticulture crops. Int J Chem Stud. 2018;6(3):3301–5.
  32. 32. Sundarraj S, Thangam R, Sreevani V, Kaveri K, Gunasekaran P, Achiraman S, et al. γ-Sitosterol induces apoptosis in MCF-7 and A549 cells. J Ethnopharmacol. 2012;141(3):803–9. https://doi.org/10.1016/j.jep.2012.03.014
  33. 33. Varsha KK, Devendra L, Shilpa G, Priya S, Pandey A, Nampoothiri KM. 2,4-Di-tert-butyl phenol as antifungal bioactive. Int J Food Microbiol. 2015;211:44–50. https://doi.org/10.1016/j.ijfoodmicro.2015.06.025
  34. 34. Cheong BE, Zakaria NA, Cheng AY, Teoh PL. GC-MS analysis of Strobilanthes crispus plants and callus. Trans Sci Technol. 2016;3(1–2):155–61.
  35. 35. Abdullah RR. Insecticidal activity of fungal secondary metabolites against cotton pests. J Plant Prot Pathol. 2019;10(12):647–53. https://doi.org/10.21608/jppp.2019.79456
  36. 36. Ayalew AA. Chromatographic determination of Lantana camara leaf oil. Int J Med Res. 2020;48(10):0300060520962344. https://doi.org/10.1177/0300060520962344
  37. 37. Ajaib M, Ishtiaq M, Shafi F, Bhatti KH, Zahid MT. Antimicrobial and antioxidant analysis of Strobilanthes glutinous. Biosci Res. 2020;17(2):1521–34.
  38. 38. Siswadi S, Saragih GS. Phytochemical analysis of Sterculia quadrifida extract. AIP Conf Proc. 2021;2353:030098. https://doi.org/10.1063/5.0053057
  39. 39. Kant K, Lal UR, Ghosh M. Antibacterial activity and GC-MS studies of Arisaema tortuosum extracts. Indian J Pharm Educ Res. 2019;53:S280–7. https://doi.org/10.5530/ijper.53.3s.98
  40. 40. Pu ZH, Zhang YQ, Yin ZQ, Xu J, Jia RY, Yang LU, et al. Antibacterial activity of fatty acid ester from neem oil. Agric Sci China. 2010;9(8):1236–40. https://doi.org/10.1016/S1671-2927(09)60212-1
  41. 41. Dordab T, Sourinejad I, Nazemi M. Antibacterial effect of squalene from Carcharhinus sorrah liver. Fish Sci Technol. 2021;10(2):251–8.
  42. 42. Femi-Adepoju A, Fatoba PO, Adepoju A, Oluyori AP. Phytochemical and antimicrobial analysis of Gleichenia pectinata. Int J Biomed. 2018;9(12):400–6.
  43. 43. Mailafiya MM, Yusuf AJ, Abdullahi MI, Aleku GA, Ibrahim IA, Yahaya M, et al. Antimicrobial activity of stigmasterol from Neocarya macrophylla. J Med Plants Econ Dev. 2018;2(1):1–5.
  44. 44. Liu K, Zhang X, Xie L, Deng M, Chen H, Song J, et al. Lupeol and derivatives as anticancer agents. Pharmacol Res. 2021;164:105373. https://doi.org/10.1016/j.phrs.2020.105373
  45. 45. Ghasemi M, Turnbull T, Sebastian S, Kempson I. The MTT assay: utility and limitations. Int J Mol Sci. 2021;22(23):12827. https://doi.org/10.3390/ijms222312827
  46. 46. Balasubramaniam G, Sekar M, Badami S. Antioxidant and cytotoxic properties of Strobilanthes kunthianus. Res J Pharm Technol. 2021;14(5):2522–8. https://doi.org/10.52711/0974-360X.2021.00444
  47. 47. Chong HZ, Rahmat A, Yeap SK, Md Akim A, Alitheen NB, Othman F, et al. Cytotoxicity of Strobilanthes crispus extract on MCF-7 cells. BMC Complement Altern Med. 2012;12:35. https://doi.org/10.1186/1472-6882-12-35
  48. 48. Ekowati N, Mumpuni A, Ratnaningtyas NI, Maharning AR. Anticancer activity of Schizophyllum commune extracts. Biodiversitas. 2020;21(12). https://doi.org/10.13057/biodiv/d211251
  49. 49. Ferdi OG, Pulat CC, Ilhan S, Atmaca H. GC-MS analysis and apoptotic effect of Paliurus spina-christi extracts. Sak Univ J Sci. 2022;26(2):357–64. https://doi.org/10.16984/saufenbilder.1029351

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