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Research Articles

Vol. 13 No. sp1 (2026): Recent Advances in Agriculture

Seed germination dynamics of Carum carvi L. under different temperature regimes and growth regulators

DOI
https://doi.org/10.14719/pst.13099
Submitted
6 December 2025
Published
08-04-2026

Abstract

Seed germination is a crucial stage in the crop life cycle. Laboratory experiments were carried out to assess the germination performance of Carum carvi L. seeds and to evaluate the effects of different temperatures and plant growth regulators on their germination behaviour. The present investigation consisted of fifteen treatment combinations, including 3 temperature levels (15, 20 and 25 °C) and 4 concentrations (25, 50, 75 and 100 ppm) of 3 chemical treatments, i.e., Gibberellic Acid (GA₃), Naphthalene Acetic Acid (NAA) and thiourea, arranged in a factorial completely randomized design (CRD). Various germination and growth parameters were recorded during the study and the results revealed that among the different treatment combinations, 20 °C combined with thiourea at 100 ppm was the most effective in enhancing all germination-related traits. The temperature of 15 °C was also found suitable, showing germination performance nearly comparable to that observed at 20 °C. Initial germination was observed after 3 days, with a mean daily germination (MDG) rate of  0.43 seeds day-1, total germination of 65.33 %, germination energy (GE) of 22 %, speed of germination of 1.16 seeds day-1, shoot length (SL) of 5.21 cm, root length (RL) of 3.50 cm and a correspondingly higher seedling vigour index (SVI). However, further studies are required to determine whether increasing thiourea concentration would exert a positive or negative influence on germination. Overall, the treatment combination of 20 °C × thiourea at the rate of 100 ppm was the most effective in enhancing seed germination performance of C. carvi.

References

  1. 1. Medani RA, Taha RS. Improving growth and yield of caraway (Carum carvi L.) plants by decapitation and/or active dry yeast application. Int J Curr Microbiol Appl Sci. 2015;4(9):47–60.
  2. 2. Dev S. Carum carvi. In: Prime Ayurvedic Plant Drugs. Cham: Springer; 2023. p. 212–17. https://doi.org/10.1007/978-3-031-22075-3_28
  3. 3. Sachan AK, Das DR, Kumar M. Carum carvi – An important medicinal plant. J Chem Pharm Res. 2016;8(3):529–33.
  4. 4. Malhotra SK. Caraway. In: Peter KV, editor. Handbook of herbs and spices. 2nd ed. Cambridge: Woodhead Publishing; 2012. p. 225–48. https://doi.org/10.1533/9780857095688.225
  5. 5. Bradley PR, editor. British herbal compendium: a handbook of scientific information on widely used plant drugs. Vol. 2. Bournemouth: British Herbal Medicine Association; 1992.
  6. 6. Laribi B, Bettaieb I, Kouki K, Sahli A, Mougou A, Marzouk B. Water deficit effects on caraway (Carum carvi L.) growth, essential oil and fatty acid composition. Ind Crops Prod. 2009;30(3):372–9. https://doi.org/10.1016/j.indcrop.2009.07.005
  7. 7. Bisht AS, Sati M, Rana S, Chauhan RS. Effect of light vs dark on seed germination of Hedychium spicatum Smith. Int J Med Plants Nat Prod. 2015;1(1):29–30.
  8. 8. Samojlik I, Lakic N, Mimica-Dukic N, Djakovic-Svajcer K, Bozin B. Antioxidant and Hepatoprotective Potential of Essential Oils of Coriander (Coriandrum sativum L.) and Caraway (Carum carvi L.) (Apiaceae). J Agric Food Chem. 2010;58(15):8848–53. https://doi.org/10.1021/jf101645n
  9. 9. Gupta A, Dubey M, Parmar M, Mahajan S, Sharma R. Evaluation of antimicrobial activity of Carum carvi seed extract against Escherichia coli and Aspergillus niger. Drug Invent Today. 2011;3:211–3.
  10. 10. Șanli A, Karadoan T, Tonguç M, Baydar H. Effects of caraway (Carum carvi L.) seed on sprouting of potato (Solanum tuberosum L.) tubers under different temperature conditions. Turk J Field Crops. 2010;15(1):54–8.
  11. 11. Wêglarz Z. Production of biennial caraway for seed and essential oil. In: Németh E, editor. Caraway: the genus Carum. London: CRC Press; 1999.
  12. 12. Tzortzakis NG. Effect of pre-sowing treatment on seed germination and seedling vigour in endive and chicory. Hortic Sci (Prague). 2009;36(3):117–25. https://doi.org/10.17221/28/2008-HORTSCI
  13. 13. Galambosi B, Peura P. Agrobotanical features and oil content of wild and cultivated forms of caraway (Carum carvi L.). J Essent Oil Res. 1996;8(4):389–97. https://doi.org/10.1080/10412905.1996.9700646
  14. 14. Nyachiro JM, Clarke FR, DePauw RM, Knox RE, Armstrong KC. Temperature effects on seed germination and expression of seed dormancy in wheat. Euphytica. 2002;126:123–7. https://doi.org/10.1023/A:1019694800066
  15. 15. Oliveira AKM, Ribeiro JWF, Pereira KCL, Silva CAA. Effects of temperature on the germination of Diptychandra aurantiaca (Fabaceae) seeds. Acta Sci Agron. 2013;35(2):203–8. https://doi.org/10.4025/actasciagron.v35i2.15977
  16. 16. Francis DV, Subhan A, Mourad AH, Abdalla AK, Ahmed ZFR. Optimizing germination conditions of ghaf seed using ZnO nanoparticle priming through Taguchi method analysis. Sci Rep. 2024;14(1):15946. https://doi.org/10.1038/s41598-024-67025-6
  17. 17. Trivedi V, Rao K, Sasidharan N, Patel DA. Influence on seed quality parameters under different temperature and artificial ageing treatment in cumin (Cuminum cyminum). Indian J Agric Sci. 2023;88(1):121–4. https://doi.org/10.56093/ijas.v88i1.79637
  18. 18. Hassanpouraghdam M, Hajisamadi AB, Khalighi A. Gibberellic acid foliar application influences growth, volatile oil and some physiological characteristics of coriander (Coriandrum sativum L.). Rom Biotechnol Lett. 2011;16(4): 6322–27.
  19. 19. Hassan FA, Alshamsi ASM, Alyafei MAS, Kurup S, Al Busaidi N, Ahmed ZFR. Enhancing germination of ghaf seeds (Prosopis cineraria L.) using sulfuric acid scarification and cytokinin. Acta Hortic. 2023;1365:39–44. https://doi.org/10.17660/ActaHortic.2023.1365.5
  20. 20. Ali HMH, Sallam DAHM. Inducing the growth and flowering of caraway (Carum carvi L.) plant. J Appl Biol Biotechnol. 2022;10(4):86–91. https://doi.org/10.7324/JABB.2022.100412
  21. 21. Synge PM. Dictionary of gardening: a practical and scientific encyclopedia of horticulture. 2nd ed. Oxford: Clarendon Press; 1974.
  22. 22. Czabator FJ. Germination value: an index combining speed and completeness of pine seed germination. For Sci. 1962;8(4):386–96. https://doi.org/10.1093/forestscience/8.4.386
  23. 23. Abdul-Baki AA, Anderson JD. Vigor determination in soybean seed by multiple criteria. Crop Sci. 1973;13(6):630–3. https://doi.org/10.2135/cropsci1973.0011183X001300060013x
  24. 24. Gomez KA, Gomez AA. Statistical procedures for agricultural research. 2nd ed. New York: John Wiley and Sons; 1984.
  25. 25. Belwal T, Bisht A, Bhatt ID, Rawal RS. Influence of seed priming and storage time on germination and enzymatic activity of selected Berberis species. Plant Growth Regul. 2015;77:189–99. https://doi.org/10.1007/s10725-015-0051-0
  26. 26. Kumar S, Malik TP, Mor VS, Kumar P. Effect of gibberellic acid on seed quality of coriander (Coriandrum sativum L.). J Pharmacogn Phytochem. 2018;7:830–2. https://doi.org/10.20546/ijcmas.2018.709.318
  27. 27. Shah SH. Physiological effects of pre-sowing seed treatment with gibberellic acid on Nigella sativa L. Acta Bot Croat. 2007;66(1):67–73.
  28. 28. Ghaseemi M, Puteh AB, Sinniah UR, Wahab ZB. Effect of different temperature regimes on seed germination in Bunium persicum (black zira or black cumin) ecotypes. Int J Agric Res Rev. 2012;2(3):240–6.
  29. 29. Verma SK, Kumar B, Ram G, Singh HP, Lal RK. Varietal effect on germination parameters at controlled and uncontrolled temperature in palmarosa (Cymbopogon martini). Ind Crops Prod. 2010;32(3):696–9. https://doi.org/10.1016/j.indcrop.2010.07.015
  30. 30. Aldasoro JJ, Matilla A, Nicolas G. Effect of ABA, fusicoccin and thiourea on germination and K+ and glucose uptake in chickpea seeds at different temperatures. Plant Physiol. 1981;53(2):139–45. https://doi.org/10.1111/j.1399-3054.1981.tb04123.x
  31. 31. El-Keblawy A, Gairola S. Dormancy regulating chemicals alleviate innate seed dormancy and promote germination of desert annuals. J Plant Growth Regul. 2016;36:300–11. https://doi.org/10.1007/s00344-016-9640-z
  32. 32. Pourreza J, Bahrani A. Estimating cardinal temperatures of milk thistle (Silybum marianum) seed germination. Am Eurasian J Agric Environ Sci. 2012;12(11):1485–9.
  33. 33. Bhardwaj AK, Kapoor S, Naryal A, Bhardwaj P, Warghat AR, Kumar B, et al. Effect of various dormancy breaking treatments on seed germination, seedling growth and seed vigour of medicinal plants. Trop Plant Res. 2016;3(3):508–16. https://doi.org/10.22271/tpr.2016.v3.i3.067
  34. 34. Ünver MC, Tilki F. Salinity, germination promoting chemicals, temperature and light effects on seed germination of Anethum graveolens L. Bulg J Agric Sci. 2012;18(6):974–80.
  35. 35. Nerson H. Seed production and germinability of cucurbit crops. Seed Sci Biotechnol. 2007;1(1):1–10.
  36. 36. Pandey H, Nandi SK, Nadeem M, Palni LMS. Chemical stimulation of seed germination in Aconitum heterophyllum Wall. and Aconitum balfouri Stapf. Seed Sci Technol. 2000;28(1):39–48.
  37. 37. Bhatt R, Shetty GR, Rajasekharan PE, Pooja DA, Ganapathi M, Nadukeri S. Effect of pre-sowing seed treatments on Aristolochia tagala Cham. Int J Curr Microbiol Appl Sci. 2020;9(10):1803–8. https://doi.org/10.20546/ijcmas.2020.910.219
  38. 38. Elhindi KM, Dewir YH, Asrar AW, Abdel-Salam E, El-Din AS, Ali M. Improvement of seed germination in three medicinal plant species by plant growth regulators. HortScience. 2016;51(7):887–91. https://doi.org/10.21273/HORTSCI.51.7.887
  39. 39. Anandhi S, Rajamani K. Studies on seed germination and growth in Gloriosa superba L. Global J Res Med Plants & Indigen Med. 2012;1(10):524–28.
  40. 40. Sharma RK, Sharma S, Sharma SS. Seed germination behavior of some medicinal plants of Lahaul and Spiti cold desert (Himachal Pradesh): implications for conservation and cultivation. Curr Sci. 2006;90(8):1113–8.
  41. 41. Dhankar DS, Singh M. Seed germination and seedling growth in aonla (Phyllanthus emblica Linn.) as influenced by gibberellic acid and thiourea. Crop Res Hisar. 1996;12(3):363–6.
  42. 42. Singh DK, Bhattacharya B, Mondal K. Role of pre-sowing seed treatments with different chemicals on germination behavior and seedling growth of jackfruit (Artocarpus heterophyllus Lam.). Environ Ecol. 2002;20(3):741–3.
  43. 43. Alipoor Z, Mahmodi S. Effect of different temperature on germination properties of fennel (Foeniculum vulgare Mill.), cannabis (Cannabis sativa L.) and sesame (Sesamum indicum L.). Iran J Seed Res. 2015;2(1):37–50.
  44. 44. Thakur A, Thakur PS, Dutt V, Thakur CL. Conservation of Podophyllum hexandrum: an endangered medicinal herb through seeds. Indian J Plant Physiol. 2010;15(2):110–6.
  45. 45. Abdalla ST, Mckelvie AD. The interaction of chilling and gibberellic acid on the germination of seeds of ornamental plants. Seed Sci Technol. 1980;8:139–44.
  46. 46. Yadav P, Srivastava S. Effect of thiourea application on root, old leaf and young leaf of two contrasting rice varieties (Oryza sativa L.) grown in arsenic contaminated soil. Environ Technol Innov. 2021;21:101368. https://doi.org/10.1016/j.eti.2021.101368
  47. 47. Thakur A, Sayal N. Effects of seed invigoration treatments on seed germination, seedling vigour and physio-biochemical characteristics of Angelica glauca Edgew. Int J Bioresour Stress Manag. 2018;9(3):353–8. https://doi.org/10.23910/IJBSM/2018.9.3.3C0640
  48. 48. Butola JS, Pant S, Samant SS. Effect of pre-sowing seed treatments in Hypericum perforatum L. Seed Res. 2007;35(2):205–209.
  49. 49. Bisht AS, Nautiyal BP, Bhatt AB. Propagation and growth performance of Hedychium spicatum Smith under different hormonal and soil treatments. Indian Forester. 2009;135(1):60–6.

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