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Effect of salinity on DNA methylation and antioxidant phenolic compounds of wild watercress (Rorippa nasturtium aquaticum)

Authors

  • Marcela Verónica Gutiérrez-Velázquez Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional unidad Durango, Instituto Politécnico Nacional, Durango 34220, México https://orcid.org/0000-0001-7587-320X
  • Norma Almaraz-Abarca Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional unidad Durango, Instituto Politécnico Nacional, Durango 34220, México https://orcid.org/0000-0003-1603-4865
  • José Antonio Ávila-Reyes Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional unidad Durango, Instituto Politécnico Nacional, Durango 34220, México https://orcid.org/0000-0001-9552-957X
  • Eli Amanda Delgado-Alvarado Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional unidad Durango, Instituto Politécnico Nacional, Durango 34220, México https://orcid.org/0000-0003-3835-9572
  • Laura Silvia González-Valdez Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional unidad Durango, Instituto Politécnico Nacional, Durango 34220, México https://orcid.org/0000-0002-2644-2435
  • Rene Torres-Ricario Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional unidad Durango, Instituto Politécnico Nacional, Durango 34220, México https://orcid.org/0000-0002-2523-6699
  • Hugo Manuel Monreal-García Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional unidad Durango, Instituto Politécnico Nacional, Durango 34220, México https://orcid.org/0000-0001-9001-8930
  • Dante Yamid Rojas-Barboza Family and Consumer Science, New Mexico State University, Las Cruces 88003, United States https://orcid.org/0000-0002-4218-1925
  • Andrés Vasavilbazo-Saucedo Facultad de Ciencias del Mar, Universidad Autónoma de Sinaloa, Mazatlán 82000, México https://orcid.org/0009-0006-1720-9654

DOI:

https://doi.org/10.14719/pst.2577

Keywords:

watercress, methylation, metabolites, stress, phenolics, antioxidant

Abstract

Epigenetic changes are involved in plant responses to stress. Cytosine methylation is one of the most important epigenetic changes, regulating gene expression. In this paper, the MSAP (methylation-sensitive amplification polymorphism) method was used to find out how the watercress (Rorippa nasturtium aquaticum) genome changed in response to 0, 60, 80, and 100 mM NaCl and how that affected phenylalanine ammonium lyase (PAL) activity, phenolic content, and antioxidant capacity. The results showed an inverse correlation between methylation levels and PAL activity and the contents of total phenolics and flavonoids, indicating salt stress-induced reprogramming of the methylation pattern of watercress, which has a negative effect on the synthesis of phenolics. The results revealed a significant decrease in phenolic contents and antioxidant activity under low and moderate salinity compared to control and an increase under strong salinity compared to moderate salinity. The findings of this study contribute to our understanding of the reprogramming of DNA methylation under salinity and its effect on watercress phenolic metabolism.

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Published

20-10-2023

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1.
Gutiérrez-Velázquez MV, Almaraz-Abarca N, Ávila-Reyes JA, Delgado-Alvarado EA, González-Valdez LS, Torres-Ricario R, Monreal-García HM, Rojas-Barboza DY, Vasavilbazo-Saucedo A. Effect of salinity on DNA methylation and antioxidant phenolic compounds of wild watercress (Rorippa nasturtium aquaticum). Plant Sci. Today [Internet]. 2023 Oct. 20 [cited 2024 Nov. 21];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2577

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