Exogenous gibberellin improves the yield and quality of basil (Ocimum basilicum L.) and chervil (Anthriscus cerefolium L.) plants grown under salinity stress conditions
DOI:
https://doi.org/10.14719/pst.2666Keywords:
Basil, chervil, phytohormone, salinity, osmoprotectantAbstract
Gibberellins play a crucial role as plant hormones in the regulation of various aspects of plant growth and development. They are involved in processes such as seed germination, breaking plant and bud dormancy, and counteracting the effects of auxin. Additionally, gibberellins promote leaf expansion, stimulate stem elongation, and contribute to flower development and fruit set. The objective of this study was to investigate the effects of gibberellic acid (GA3) treatments (T0: 0 ppm, T1: 1 ppm, and T2: 10 ppm) on the growth regulation and physiological parameters of basil and chervil plants under salinity stress conditions (150 mM NaCl). The study explored various growth outcomes and biochemical parameters, including chlorophyll, proteins, soluble sugars, proline, and nitrate. The results indicate that the application of gibberellic acid alleviated the adverse effects of high salinity and resulted in enhanced biomass production. In comparison to the control treatment, foliar surface values for basil and chervil increased by 15% and 35%, respectively, in T2. Moreover, root lengths of basil and chervil reached their highest values in T2, showing a 16% increase for basil and a 33% increase for chervil. Carotenoid levels were positively influenced by GA3 treatments, reaching high concentrations in T2, exceeding T0 levels by 41% for basil and 83% for chervil. Additionally, under T2 treatment, protein and glucose levels increased by factors of 2.7 and 1.7, respectively, in basil plants and by factors of 2.1 and 1.7, respectively, in chervil plants. The application of gibberellic acid led to a 33% reduction in proline content for basil and a 27% reduction for chervil compared to the T0 treatment.
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Baghour M, Akodad M, Dariouche A, Maach M, Haddaji HE, Mou-men A et al. Gibberellic acid and indole acetic acid improves salt tolerance in transgenic tomato plants overexpressing LeNHX4 antiporter. Gesunde Pflanz. 2023 Jun 1;75(3):687-93. https://doi.org/10.1007/s10343-022-00734-y
Baghour M, Gálvez FJ, Sánchez ME, Aranda MN, Venema K, Rodríguez-Rosales MP. Overexpression of LeNHX2 and SlSOS2 increases salt tolerance and fruit production in double trans-genic tomato plants. Plant Physiol Biochem. 2019 Feb 1;135:77-86. https://doi.org/10.1016/j.plaphy.2018.11.028
Maach M, Rodríguez-Rosales MP, Venema K, Akodad M, Moumen A, Skalli A et al. Improved yield, fruit quality and salt resistance in tomato co-overexpressing LeNHX2 and SlSOS2 genes. Physiol Mol Biol Plants. 2021 Apr 1;27(4):703-12. https://doi.org/10.1007/s12298-021-00974-8
Maach M, Baghour M, Akodad M, Gálvez FJ, Sánchez ME, Aranda MN et al. Overexpression of LeNHX4 improved yield, fruit quality and salt tolerance in tomato plants (Solanum lycopersicum L.). Mol Biol Rep. 2020 Jun 1;47(6):4145-53. https://doi.org/10.1007/s11033-020-05499-z
Haddaji HE, Akodad M, Skalli A, Moumen A, Baghour M. Effect of salinity on germination, growth and osmoprotectants in dill plants Anethum graveolens. PLANT CELL Biotechnol Mol Biol. 2021 Aug 26;86-95.
Raza A, Charagh S, Salehi H, Abbas S, Saeed F, Poinern GEJ et al. Nano-enabled stress-smart agriculture: Can nanotechnology deliver drought and salinity-smart crops? J Sustain Agric Envi-ron. 2023;2(3):189-214. https://doi.org/10.1002/sae2.12061
Oumara NGA, El Youssfi L. Salinization of soils and aquifers in Morocco and the alternatives of response. Environ Sci Proc. 2022;16(1):65. https://doi.org/10.3390/environsciproc2022016065
Raza A, Tabassum J, Fakhar AZ, Sharif R, Chen H, Zhang C et al. Smart reprograming of plants against salinity stress using mod-ern biotechnological tools. Crit Rev Biotechnol. 2022;0(0):1-28.
Muluneh MG. Impact of climate change on biodiversity and food security: A global perspective—a review article. Agric Food Se-cur. 2021 Sep 6;10(1):36. https://doi.org/10.1186/s40066-021-00318-5
Maroušek J, Maroušková A, Periakaruppan R, Gokul GM, Anbukumaran A, Bohatá A et al. Silica nanoparticles from coir pith synthesized by acidic sol-gel method improve germination economics. Polymers. 2022 Jan;14(2):266. https://doi.org/10.3390/polym14020266
Hedden P. Gibberellin biosynthesis in higher plants. In: Annual Plant Reviews. [Internet]. John Wiley & Sons, Ltd. 2016 [cited 2023 Sep 23];49:p. 37-72. https://doi.org/10.1002/9781119210436.ch2
Hussain S, Hafeez MB, Azam R, Mehmood K, Aziz M, Ercisli S et al. Deciphering the role of phytohormones and osmolytes in plant tolerance against salt stress: Implications, possible cross-talk and prospects. J Plant Growth Regul. 2023 Jul 15;1-22. https://doi.org/10.1007/s00344-023-11070-4
Samad A, Shaukat K, Ansari MUR, Nizar M, Zahra N, Naz A et al. Role of foliar spray of plant growth regulators in improving pho-tosynthetic pigments and metabolites in Plantago ovata (Psyllium) under salt stress – A field appraisal. Biocell. 2023;47(3):523-32. https://doi.org/10.32604/biocell.2023.023704
Ullah I, Dawar K, Tariq M, Sharif M, Fahad S, Adnan M et al. Gib-berellic acid and urease inhibitor optimize nitrogen uptake and yield of maize at varying nitrogen levels under changing climate. Environ Sci Pollut Res. 2022 Jan 1;29(5):6568-77. https://doi.org/10.1007/s11356-021-16049-w
Bhattacharyya R, Ghosh BN, Mishra PK, Mandal B, Rao CS, Sarkar D et al. Soil degradation in India: Challenges and poten-tial solutions. Sustainability. 2015 Apr;7(4):3528-70. https://doi.org/10.3390/su7043528
Eyidogan F, Oz MT, Yucel M, Oktem HA. Signal transduction of phytohormones under abiotic stresses. In: Phytohormones and abiotic stress tolerance in plants [Internet]. Springer, Berlin, Heidelberg. 2012 [cited 2023 Sep 23]; p. 1-48. https://doi.org/10.1007/978-3-642-25829-9_1
Wang YH, Zhang G, Chen Y, Gao J, Sun YR, Sun MF et al. Exoge-nous application of gibberellic acid and ascorbic acid improved tolerance of okra seedlings to NaCl stress. Acta Physiol Plant. 2019 Jun 1;41(6):1-10. https://doi.org/10.1007/s11738-019-2869-y
Hoang HT, Moon JY, Lee YC. Natural antioxidants from plant extracts in skincare cosmetics: Recent applications, challenges and perspectives. Cosmetics. 2021 Dec;8(4):106. https://doi.org/10.3390/cosmetics8040106
Salmerón-Manzano E, Garrido-Cardenas JA, Manzano-Agugliaro F. Worldwide research trends on medicinal plants. Int J Environ Res Public Health. 2020 Jan;17(10):3376. https://doi.org/10.3390/ijerph17103376
Srivastava N, Ranjana, Singh S, Gupta AC, Shanker K, Bawankule DU et al. Aromatic ginger (Kaempferia galanga L.) extracts with ameliorative and protective potential as a functional food, be-yond its flavor and nutritional benefits. Toxicol Rep. 2019 Jan 1;6:521-28. https://doi.org/10.1016/j.toxrep.2019.05.014
Prinsloo G, Nogemane N, Street R. The use of plants containing genotoxic carcinogens as foods and medicine. Food Chem Toxi-col. 2018 Jun 1;116:27-39. https://doi.org/10.1016/j.fct.2018.04.009
Beltrán-Noboa A, Proaño-Ojeda J, Guevara M, Gallo B, Berrueta LA, Giampieri F et al. Metabolomic profile and computational analysis for the identification of the potential anti-inflammatory mechanisms of action of the traditional medicinal plants Oci-mum basilicum and Ocimum tenuiflorum. Food Chem Toxicol. 2022 Jun 1;164:113039. https://doi.org/10.1016/j.fct.2022.113039
Liopa-Tsakalidi A, Barouchas PE. Salinity, chitin and GA3 effects on seed germination of chervil (Anthriscus cerefolium). Aust J Crop Sci. 2011;5(8):973-78.
Pavolová H, Tomáš, Kyše?a K, Klimek M, Hajduová Z, Zawada M. The analysis of investment into industries based on portfolio managers. Acta Montan Slovaca. 2021 May 20;(26):161-70. https://doi.org/10.46544/AMS.v26i1.14
Maroušek J, Minofar B, Maroušková A, Strunecký O, Gavurová B. Environmental and economic advantages of production and application of digestate biochar. Environ Technol Innov. 2023 May 1;30:103109. https://doi.org/10.1016/j.eti.2023.103109
Lichtenthaler HK. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. In: Methods in enzymology [Internet]. Academic Press. 1987 [cited 2022 Apr 19];p. 350-82. (Plant Cell Membranes; vol. 148). Available from: https://www.sciencedirect.com/science/article/pii/0076687987480361
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72(1):248-54. https://doi.org/10.1006/abio.1976.9999
Irigoyen JJ, Einerich DW, Sánchez-Díaz M. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol Plant. 1992;84(1):55-60. https://doi.org/10.1111/j.1399-3054.1992.tb08764.x
Paquin R, Lechasseur P. Observations sur une méthode de dos-age de la proline libre dans les extraits de plantes. Can J Bot. 1979 Sep 15;57(18):1851-54. https://doi.org/10.1139/b79-233
Khan MIR, Iqbal N, Masood A, Khan NA. Variation in salt toler-ance of wheat cultivars: Role of glycinebetaine and ethylene. Pedosphere. 2012 Dec 1;22(6):746-54. https://doi.org/10.1016/S1002-0160(12)60060-5
Misra N, Gupta AK. Effect of salt stress on proline metabolism in two high yielding genotypes of green gram. Plant Sci. 2005 Aug 1;169(2):331-39. https://doi.org/10.1016/j.plantsci.2005.02.013
Vishal B, Kumar PP. Regulation of seed germination and abiotic stresses by gibberellins and abscisic acid. Front Plant Sci [Internet]. 2018 [cited 2023 Sep 23];9. https://doi.org/10.3389/fpls.2018.00838
Achard P, Gong F, Cheminant S, Alioua M, Hedden P, Genschik P. The cold-inducible CBF1 factor–dependent signaling pathway modulates the accumulation of the growth-repressing DELLA proteins via its effect on gibberellin metabolism. Plant Cell. 2008 Aug 1;20(8):2117-29. https://doi.org/10.1105/tpc.108.058941
Colebrook EH, Thomas SG, Phillips AL, Hedden P. The role of gibberellin signalling in plant responses to abiotic stress. J Exp Biol. 2014 Jan 1;217(1):67-75. https://doi.org/10.1242/jeb.089938
Khan AL, Waqas M, Lee IJ. Resilience of Penicillium resedanum LK6 and exogenous gibberellin in improving Capsicum annuum growth under abiotic stresses. J Plant Res. 2015 Mar 1;128(2):259-68. https://doi.org/10.1007/s10265-014-0688-1
Matos FS, Freitas IAS, Pereira VLG, Pires WKL. Effect of gibberel-lin on growth and development of Spondias tuberosa seedlings. Rev Caatinga. 2020 Nov 23;33:1124-30. https://doi.org/10.1590/1983-21252020v33n427rc
Taïz L, Zeiger E. Fisiologia végétale. 5 éd. Porto AlegreA : rtmed. 2013;954p.
Amaro, Camila Lariane, Cunha, Stephany Diolino, Grupioni, Pedro Henrique França et al. Análise do crescimento de mudas de Eucalyptus sp. submetidas a diferentes doses de giberelina. Agri-environmental Sciences. 2017;3(1):24-29. https://doi.org/10.36725/agries.v3i1.452
Raza Gurmani A, Wang X, Rafique M, Jawad M, Raza Khan A, Ullah Khan Q et al. Exogenous application of gibberellic acid and silicon to promote salinity tolerance in pea (Pisum sativum L.) through Na+ exclusion. Saudi J Biol Sci. 2022 Jun 1;29(6):103305. https://doi.org/10.1016/j.sjbs.2022.103305
Ahmad P. Growth and antioxidant responses in mustard (Brassica juncea L.) plants subjected to combined effect of gib-berellic acid and salinity. Arch Agron Soil Sci. 2010 Oct 1;56(5):575-88. https://doi.org/10.1080/03650340903164231
Sun T, Rao S, Zhou X, Li L. Plant carotenoids: Recent advances and future perspectives. Mol Hortic. 2022 Dec;2(1):1-21. https://doi.org/10.1186/s43897-022-00023-2
XiaoBo G, YuanNong L, YaDan D, GuoJun W, ChangMing Z, QuanMao R et al. Compensative impact of winter oilseed rape (Brassica napus L.) affected by water stress at re-greening stage under different nitrogen rates. Zhongguo Shengtai Nongye Xuebao Chin J Eco-Agric. 2016;24(5):572-81.
Shahzad K, Hussain S, Arfan M, Hussain S, Waraich EA, Zamir S et al. Exogenously applied gibberellic acid enhances growth and salinity stress tolerance of maize through modulating the mor-pho-physiological, biochemical and molecular attributes. Bio-molecules. 2021 Jul;11(7):1005. https://doi.org/10.3390/biom11071005
Du X, Su M, Jiao Y, Xu S, Song J, Wang H et al. A transcription factor SlNAC10 gene of Suaeda liaotungensis regulates proline synthesis and enhances salt and drought tolerance. Int J Mol Sci. 2022 Jan;23(17):9625. https://doi.org/10.3390/ijms23179625
Gharsallah C, Fakhfakh H, Grubb D, Gorsane F. Effect of salt stress on ion concentration, proline content, antioxidant en-zyme activities and gene expression in tomato cultivars. AoB PLANTS. 2016 Jan 1;8:plw055. https://doi.org/10.1093/aobpla/plw055
Silva-Ortega CO, Ochoa-Alfaro AE, Reyes-Agüero JA, Aguado-Santacruz GA, Jiménez-Bremont JF. Salt stress increases the expression of p5cs gene and induces proline accumulation in cactus pear. Plant Physiol Biochem. 2008;1(46):82-92. https://doi.org/10.1016/j.plaphy.2007.10.011
Fougère F, Le Rudulier D, Streeter JG. Effects of salt stress on amino acid, organic acid and carbohydrate composition of roots, bacteroids and cytosol of Alfalfa (Medicago sativa L.) 1. Plant Physiol. 1991 Aug 1;96(4):1228-36. https://doi.org/10.1104/pp.96.4.1228
Gangopadhyay G, Basu S, Mukherjee BB, Gupta S. Effects of salt and osmotic shocks on unadapted and adapted callus lines of tobacco. Plant Cell Tissue Organ Cult. 1997 Apr 1;49(1):45-52. https://doi.org/10.1023/A:1005860718585
Madan S, Nainawatee HS, Jain RK, Chowdhury JB. Proline and proline metabolising enzymes in in-vitro selected NaCl-tolerant Brassica juncea L. under salt stress. Ann Bot. 1995 Jul 1;76(1):51-77. https://doi.org/10.1006/anbo.1995.1077
Angrish R, Kumar B, Datta KS. Effect of gibberellic acid and ki-netin on nitrogen content and nitrate reducatase activity in wheat under saline conditions. Indian J Plant Physiol. 2001;6(2):172-77.
Wang Y, Yao Q, Zhang Y, Zhang Y, Xing J, Yang B et al. The role of gibberellins in regulation of nitrogen uptake and physiological traits in maize responding to nitrogen availability. Int J Mol Sci. 2020 Jan;21(5):1824.
Vetrano F, Moncada A, Miceli A. Use of gibberellic acid to in-crease the salt tolerance of leaf lettuce and rocket grown in a floating system. Agronomy. 2020 Apr;10(4):505. https://doi.org/10.3390/agronomy10040505
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Copyright (c) 2022 Hamza El haddaji, Mustapha Akoudad, Ali Skalli, Abdelmajid Moumen, Said Bellahcen, Mostapha Maach, Abderrahmane rahhou, Mourad Baghour
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