Effects of electromagnetic waves on parameters, hydration and in vitro antimicrobial activity of the Brassica oleracea L. var. italica Plenck. and water

Authors

DOI:

https://doi.org/10.14719/pst.2987

Keywords:

Electromagnetic waves, osmosis, antimicrobial action, Brassica oleracea L. var. italica Plenck

Abstract

Our team has developed a device capable of emitting electromagnetic waves via a solenoid. Building on this breakthrough, our research endeavors to explore the effects of these waves on the weight and osmotic parameters of Brassica oleracea L. var. italic Plenck, specifically broccoli sprouts. These sprouts were subjected to various types of activated water infused with electromagnetic waves, with their weight measured in grams and their percentage composition analyzed. This study aims to elucidate the potential impact of electromagnetic waves on agricultural practices and nutritional outcomes. The findings present valuable insights into optimizing crop growth and enhancing nutritional value, thus holding promise for agricultural applications. In addition to nutritional aspects, contamination by pathogenic microorganisms poses a significant challenge in fruit and vegetable cultivation, particularly when organic fertilizers are employed. Mitigating microbial contamination in plant-based foods is paramount for preventing gastrointestinal and other infections, thereby safeguarding consumer health. In vitro studies were conducted to investigate the antimicrobial effects of electromagnetic waves across different frequencies. Water suspensions containing a density of 105 cells per milliliter of three microbial ATCC strains—Escherichia coli, Staphylococcus aureus, and Candida albicans—were utilized. These suspensions were exposed to electromagnetic radiation within the frequency ranges of 350-600 Hz (designated as frequencies F1) and 20-40 kHz (designated as frequencies F2), as well as combinations of both F1 and F2 frequencies, each lasting 15 minutes. Results indicated that the tested strains exhibited heightened sensitivity to exposures at 350-600 Hz and 20-40 kHz, leading to a decrease in cell viability by approximately 70% compared to untreated controls.

Downloads

Download data is not yet available.

References

Acikgoz FE. Influence of different sowing times on mineral composition and vitamin C of some broccoli (Brassica oleracea var.italica) cultivars. Scientific Research and Essays. 2011;6(4):760-65. https://DOI: 10.5897/SRE10.594

Montaner C, Mallor C, Laguna S,Zufiaurre R. Bioactive compounds, antioxidant activity and mineral content of bróquil: A traditional Brassica oleracea var. italica crop. Front Nutr Sec Food Chemistry. 2023;9(1006012):1-14. https://doi: 10.3389/fnut. 2022; 1006012

Li H, Xia Y, Liu H-Y et al. Nutritional values, beneficial effects and food applications of broccoli (Brassica oleracea var. italica). Trends in Food Science and Technology. 2022;119:288-308. https://doi.org/10.1016/j.tifs.2021.12.015

Gramatikov P, Antonov A, Gramatikova MA. Study of water systems' properties and structure variations under the stimulus of outside influences. Fresenius JAnalChem. 1992;343:134-35.https://doi.org/10.1007/BF00332070

Todorova L, Antonov A. Note on the drop evaporation method for study of water hydrogen bond distribution: I. An application for filtration. Comptes Rendus de l'Académie Bulgare des Sciences. 2000;53:43-46.

Ignatov I, Iliev MT, Gramatikov PS. Education program onphysics and chemistry for non-equilibrium processes at the interfaces between solid-liquid-gaseous media. European Journal of Contemporary Education. 2023;12(3):862-73.https://doi.org/10.13187/ejced.2023.3.862

Iliev MT, Huether F, Ignatov I, Gramatikov PS. Education of students on physics and chemistry with effects of water filtration. Modeling of water clusters and hexagonal structures.European Journal of Contemporary Education. 2023;12(4): pp. 1546-60.https://doi.org/10.13187/ejced.2023.4.1546

Ignatov I, Huether F, Neshev N et al. Research of water molecules cluster structuring during Haberlea rhodopensis Friv. hydration. Plants. 2022;11:2655. https://doi.org/10.3390/plants11192655

Ignatov I, Popova T. Applications of Moringa oleifera Lam., Urtica dioica L., Malva sylvestris L. and Plantago major L. containing potassium for recovery. Plant Cell Biotechnol Mol Biol J. 2021;22(7-8):93-103.

Ignatov I, Popova TP, Bankova R, Neshev N. Spectral analyses of fresh and dry Hypericum perforatum L. Effects with colloidal nano silver 30 ppm. Plant Science Today. 2022;9:41-47.https://doi.org/10.14719/pst.1429

Ignatov I, Neshev N, Popova TP et al. Theoretical analysis of hydrogen bonds, energy distribution and information in a 1 % Rosa damascena Mill oil solution. Plant Science Today. 2022;9:760-65.https://doi.org/10.14719/pst.1645

Ignatov I, Balabanski V, Angelcheva M. Application of infrared spectral analyses for medicinal plants containing Calcium (Ca2+). Plant Science Today. 2022;9:1066-73.https://doi.org/10.14719/pst.1738

Bera K, Dutta P, Sadhukan S. Seed priming with non-ionizing physical agents: Plant responses and underlying physiological mechanisms. Plant Cell Rep. 2022;41:53-73. https://doi.org/10.1007/s00299-021-02798-y

Ignatov I, Mosin OV, Niggli H et al. Evaluating of possible methods and approaches for registering of electromagnetic waves emitted from the human body. Advances in Physics Theories and Applications. 2015;30:15-33.

Ayrapetyan S. The role of cell hydration in realizing biological effects of non-ionizing radiation. Electromagn Biol Med. 2015;34(3):197-210. https://doi.org/10.3109/15368378.2015.1076443

Sanders GJ, Arndt SK. Osmotic adjustment under drought conditions. Plant Responses to Drought Stress. 2012;199-229. https://doi.org/10.1007/978-3-642-32653-0_8

Mehandjiev D, Ignatov I, Neshev N et al. History-dependent hydrogen bonds energy distributions in NaCl aqueous solutions undergoing osmosis and diffusion through a ceramic barrier.JChemTechnolMetall. 2023;58(2):40-346.https://doi.org/10.59957/jctm.v58i2.59

Popova TP. Microbiology. University of Forestry. 2016;1-299. [in Bulgarian]

Popova TP, Ignatov I. In vitro antimicrobial activity of colloidal nano silver. BulgJVetMed. 2023;26(2):168-81. https://doi.org/10.15547/bjvm.2411

Felhofer M, Mayr K, Lutz-Meindl U et al. Raman imaging of Micrasterias: New insights into shape formation. Protoplasma. 2021;258:1323-34. https://doi.org/10.1007/s00709-021-01685-3

Inhan-Garip A, Aksu B, Akan Z et al. Effect of extremely low-frequency electromagnetic fields on growth rate and morphology of bacteria. Int J Radiat Biol. 2011;87(12):1155-61.https://doi.org/10.3109/09553002.2011.560992

Kitahara Y, Van Teeffelen S. Bacterial growth – from physical principles to autolysis. Current Opinion in Microbiology. 2023;74:102326. https://DOI: 10.1016/j.mib.2023.102326

Garuba EO, Ajunwa OM, Ibrahim-King AN. Evaluation of the effects of sound exposure and low field electromagnetism on growth and antibiotics susceptibility of some microorganisms. Bull Natl Res Cent. 2021;45:216. https://doi.org/10.1186/s42269-021-00674-z

Taheri M, Moradi M, Mortazavi S et al. Evaluation of the 900 MHz radiofrequency radiation effects on the antimicrobial susceptibility and growth rate of Klebsiella pneumoniae. Shiraz E-Med J. 2017;18(3):e44946. https://doi.org/10.17795/semj44946

Yepes-Molina L, Carbajal M, Martinez-Ballesta MC. Detergent resistant membrane domains in broccoli plasma membrane associated to the response to salinity stress. Int J Mol Sci. 2020;21(20):7694. https://doi.org/10.3390/ijms21207694

Ignatov I. Review of different types of mountain springs and mineral waters from Bulgaria based on their natural origin and health benefits. MedicniPerspektivi. 2023;51(4):199-206.https://doi.org/10.26641/2307-0404.2023.4.294236

Published

22-04-2024 — Updated on 20-05-2024

Versions

How to Cite

1.
Ignatov I, Huether F, P. Popova T, I. Ignatov A, T. Iliev M, Stoyanov C. Effects of electromagnetic waves on parameters, hydration and in vitro antimicrobial activity of the Brassica oleracea L. var. italica Plenck. and water. Plant Sci. Today [Internet]. 2024 May 20 [cited 2024 Nov. 21];11(2). Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2987

Issue

Section

Research Articles

Most read articles by the same author(s)