Research on the structuring of water clusters in Chlorella vulgaris water suspension

Christos Drossinakis; Sadek Baiti; Stoil D. Karadzhov; Nedyalka Valcheva; Ivan Angushev; Mariana Angelcheva; Fabio Huether; Irina Gotova; Zhechko Dimitrov; Alexander I. Ignatov; Ignat Ignatov; Mila  D. Kaleva; Toshka E. Petrova; Mario T. Iliev; Nikolai Neshev; Ralitsa Bankova; Veronika  Deleva; Tanya  Toshkova-Yotova; Teodora P. Popova

doi:10.14719/pst.2493

Authors

Christos Drossinakis IAWG- INTERNATIONALE Akademie für Wissenschaftliche Geistheilung, Frankfurt, Germany; Hauptplatz 44, 2293 Marchegg 2293, Austria https://orcid.org/0000-0002-6055-3040
Sadek Baiti Nature Bioresearch, Fougeres 35300, France https://orcid.org/0000-0001-7074-4193
Stoil D. Karadzhov Bulgarian Association of Activated Waters, Sofia 1619, Bulgaria https://orcid.org/0000-0002-9472-9227
Nedyalka Valcheva Trakia University, Department of Biological Sciences, Stara Zagora 6015, Bulgaria https://orcid.org/0000-0001-9038-5562
Ivan Angushev Medical University-Sofia, Sofia 1606, Bulgaria https://orcid.org/0000-0002-2933-8051
Mariana Angelcheva Department of Physiotherapy and Rehabilitation", Faculty of Public Health, Healthcare and Tourism, National Sports Academy "V. Levski”, Sofia 1700, Bulgaria https://orcid.org/0000-0002-9697-6088
Fabio Huether EVODROP AG, Zurich, Brüttisellen 8306, Switzerland https://orcid.org/0000-0002-2087-5678
Irina Gotova LB Bulgaricum PLCLB Bulgaricum Plc, R&D Center, Sofia 1113, Bulgaria https://orcid.org/0000-0002-9068-1481
Zhechko Dimitrov LB Bulgaricum Plc, R&D Center, Sofia 1113, Bulgaria https://orcid.org/0000-0001-6945-4025
Alexander I. Ignatov Scientific Research Center of Medical Biophysics (SRCMB), Sofia 1111, Bulgaria https://orcid.org/0000-0001-6578-2523
Ignat Ignatov Scientific Research Center of Medical Biophysics (SRCMB), Sofia 1111, Bulgaria https://orcid.org/0000-0002-4761-2982
Mila D. Kaleva The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Akad. G. Bonchev Str., 1113 Soﬁa, Bulgaria https://orcid.org/0000-0002-1740-5296
Toshka E. Petrova University of Forestry, Faculty of Veterinary Medicine, 10 Kl. Ohridski Blvd., Sofia 1756, Bulgaria https://orcid.org/0000-0002-4761-4929
Mario T. Iliev Faculty of Physics, Sofia University "St. Kliment Ohridski", Sofia 1000, Bulgaria https://orcid.org/0000-0003-4738-4550
Nikolai Neshev Faculty of Physics, Sofia University "St. Kliment Ohridski", Sofia 1000, Bulgaria https://orcid.org/0000-0002-8596-9392
Ralitsa Bankova Department of Internal Noncommunicable Diseases, Pathology and Pharmacology, Faculty of Veterinary Medicine, University of Forestry, 10 Kl. Ohridski Blvd., Sofia 1756, Bulgaria https://orcid.org/0000-0001-7326-1920
Veronika Deleva Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria https://orcid.org/0009-0009-0514-3063
Tanya Toshkova-Yotova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria https://orcid.org/0000-0002-2394-2282
Teodora P. Popova University of Forestry, Faculty of Veterinary Medicine, 10 Kl. Ohridski Blvd., Sofia 1756, Bulgaria https://orcid.org/0000-0003-4330-7996

DOI:

https://doi.org/10.14719/pst.2493

Keywords:

Chlorella vulgaris, Non-equilibrium energy spectrum analysis, Differential non-equilibrium energy spectrum analysis, cluster structuring

Abstract

Many bioactive compounds of natural origin have beneficial effects on human health and are used to treat different diseases. Chlorella is a genus of green algae with a high potential for producing biologically active substances. Exposure to extreme conditions can enhance its antioxidant activity and the production of concrete metabolites. C. vulgaris is cultivated in plantations. It is accessible in pharmacies and drugstores. The Health Act of 2005 in Bulgaria allows the therapeutic and prophylactic use of herbs, both independently by patients and as prescribed by a doctor. This study performed comparative spectral analyses of C. vulgaris using a 1% suspension of C. vulgaris in deionized water (v/v) by the methods of Non-equilibrium energy spectrum (NES) and Differential non-equilibrium energy spectrum (DNES). The research was performed in order to make indirect studies of the biological effects of C. vulgaris, which are connected with calcium conductivity and anti-inflammatory and anti-tumor effects. The effects of structuring of water clusters by C. vulgaris were examined. The data from spectral analyses, connected with a peak at (E =-0.1312 eV)(?=9.45 ?m) (?=1058 cm-1), revealed anti-inflammatory effects. The anti-oxidant and anti-tumor effects of C. vulgaris were shown at (E=-0.1387 eV)(?=8.95 ?m)(?=1117 cm-1). The results showed effects of improvement of calcium conductivity and anti-inflammatory, antioxidant and antitumor effects of C. vulgaris on human health.

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