Efficient synthesis of plant-mediated silver nanoparticles and their screening for antimicrobial activity
DOI:
https://doi.org/10.14719/pst.2017.4.3.328Keywords:
Nanobiotechnology, Antimicrobial activity, Plant extract, Silver nano particlesAbstract
Now days, the development of safe, cost effective, reliable and eco-friendly processes for the synthesis of nanoparticles is an important aspect of nanotechnology. Among the various agents, plants show immense potential for the synthesis of nanoparticles. The bio-molecules found in plants induce reduction of Ag+ ions from silver nitrate to silver nanoparticles (AgNPs); therefore, in the present work, the aqueous leaves extract of the plant was used as reducing agent for the synthesis of silver nanoparticles. We synthesized extracellular silver nanoparticles using extract of the leaves of four different medicinal plants which act as a reducing agent at room temperature. The characteristic color change was observed on addition of plant extract to the silver nitrate solution due to their specific properties (Surface Plasmon Resonance). UV-Vis spectroscopy was used for the characterization of the silver nanoparticles. Green synthesized nanoparticles are evaluated for their antimicrobial activity against the Gram-positive and Gram-negative bacteria as well as two pathogenic fungi Aspergillus fumigatus and Curvularia lunata. The silver nanoparticles (SNPs) of selected plant parts have shown more toxicity towards bacterial species than that of the fungal species. Comparing with simple plant extracts, the SNPs exhibited greater antimicrobial efficacy and advantage over conventional antibiotics to which these microorganisms usually impart resistance.
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References
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28. Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, Fatma A. Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids Surf B. 2010; 81: 81-6. https://doi.org/10.1016/j.colsurfb.2010.06.029
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31. Jain D, Kumar D, Kachhwaha S, Kothari S. Synthesis of plant mediated silver nanoparticles using Papaya Fruit Extract and Evaluation of their Antimicrobial Activities. Digest. Journal of Nanomaterials and Biostructures. 2009; 4:557-63.
32. Rai A, Singh A, Sastry M. Role of halide ions and temperature on the morphology of biologically synthesized gold nanotriangles. Langmuir. 2006; 22:736-41. https://doi.org/10.1021/la052055q
33. Noginov MA, Zhu G, Bahuoura M, Adegoke J, Ritzo BA. The effect of gain and absorption on surface Plasmon in metal nanoparticles. Appl. Phys. B. 2006; 86:455-60. https://doi.org/10.1007/s00340-006-2401-0
34. Roopan S, Rohit M, Rahuman A,. Kamaraj C, Bharathi A. Low-cost and ecofriendly phyto-synthesis of silver nanoparticles using Cocos nucifera coir extract and its larvicidal activity. Industrial Crops and Products. 2013; 43:631-5. https://doi.org/10.1016/j.indcrop.2012.08.013
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44. Vivek M, Kumar PS, Steffi S, Sudha S. Biogenic silver nanoparticles by Gelidiella acerosa extract and their antifungal effects. Avicenna J. Med. Biotechnol. 2011; 3:143.
45. Dibrov P, Dzioba J, Gosink KK, Hase CC. Chemiosmotic mechanism of antimicrobial activity of Ag (+) in Vibrio cholerae. Antimicrob. Agents Chemother. 2002; 46: 2668-70. https://doi.org/10.1128/AAC.46.8.2668-2670.2002
46. Kumar KM, Mandal BK, Sinha M, Krishnakumar V. Terminalia chebula mediated green and rapid synthesis of gold nanoparticles. Spectrochim. Acta A: Mol. Biomol. Spectrosc. 2012; 86:490-494. https://doi.org/10.1016/j.saa.2011.11.001
47. Udayakumar R, Velmurugan K, Srinivasan D, Krishna RR. Phytochemical and antimicrobial studies of extracts of Solanum Xanthocarpum. Ancient Science of Life. 2003; 23:1-7.
48. Saha S, Dhanasekaran D, Chandraleka S, Panneerselvam A. Synthesis, characterization and antimicrobial activity of cobalt metal complex against multi drug resistant bacterial and fungal pathogens. Facta Universitatis Series Phys. Chem. Technol. 2009; 7:73-80. https://doi.org/10.2298/FUPCT0901073S
2. Konishi Y, Ohno K, Saitoh N. Bioreductive deposition of platinum nanoparticles on the bacterium Shewanella algae. Journal of Biotechnology. 2007;128:648-53. https://doi.org/10.1016/j.jbiotec.2006.11.014
3. Willner I, Baron R, Willner B. Growing metal nanoparticles by enzymes. Advanced Materials. 2006; 18:1109-20. https://doi.org/10.1002/adma.200501865
4. Shankar SS, Rai A, Ankamwar B, Singh A, Ahmad A, Sastry M. Biological synthesis of triangular gold nanoprisms. Nature Materials. 2004; 3: 482-8. https://doi.org/10.1038/nmat1152
5. Veerasamy R, Xin TZ, Gunasagaran S. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. Journal of Saudi Chemical Society. 2011;15: 113-20. https://doi.org/10.1016/j.jscs.2010.06.004
6. Dubey SP, Lahtinen M, Sillanpaa M. Tansy fruit mediated greener synthesis of silver and gold nanoparticles. Process Biochemistry. 2010; 45: 1065-71. https://doi.org/10.1016/j.procbio.2010.03.024
7. Smitha SL, Philip D, Gopchandran KG. Green synthesis of gold nanoparticles using Cinnamomum zeylanicum leaf broth. Spectrochimica Acta Part A. 2009; 74: 735-9. https://doi.org/10.1016/j.saa.2009.08.007
8. Knoll B, Keilmann F. Near-field probing of vibrational absorption for chemical microscopy. Nature. 1999; 399: 134-7. https://doi.org/10.1038/20154
9. Sengupta S, Eavarone D, Capila I, Zhao G L, Watson N, Kiziltepe T. Temporal targeting of tumour cells and neovasculature with a nanoscale delivery system. Nature. 2005; 436: 568-72. https://doi.org/10.1038/nature03794
10. Wiley BJ, Sun Y, Xia Y. Synthesis of silver nanostructures with controlled shapes and properties, Acc Chem Res. 2007; 40: 1067-76. https://doi.org/10.1021/ar7000974
11. Kumar V, Yadav SK. Plant-mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol. 2009; 84: 151-7. https://doi.org/10.1002/jctb.2023
12. Lee HJ, Yeo SY, Jeong SH. Antibacterial effect of nanosized silver colloidal solution on textile fabrics. J. Mater. Sci. 2003; 38: 2199-2204. https://doi.org/10.1023/A:1023736416361
13. Raffi M, Hussain F, Bhatti TM, Akhter JI, Hameed A, Hasan, MM. Antibacterial characterization of silver nanoparticles against E. coli ATCC-15224, J. Mater. Sci. Technol. 2008; 24: 192-196.
14. Ahmad MB, Shameli K, Darroudi M, Yunus WMZW, Ibrahim NA, Hamid AA, Zargar M. Antibacterial activity of silver/clay/chitosan bionanocomposites. Res. J. Biol Sci. 2009; 4:1156-61.
15. Jiang H, Manolache S, Wong ACL, Denes FS. Plasma-enhanced deposition of silver nanoparticles on to polymer and metal surfaces for the generation of antimicrobial characteristics. Journal of Applied Polymer Science. 2004; 93:1411-22. https://doi.org/10.1002/app.20561
16. Parikh DV, Fink T, Rajasekharan K. Antimicrobial silver/sodium carboxymethyl cotton dressings for burn wounds. Textile Research Journal. 2005; 75:134-8. https://doi.org/10.1177/004051750507500208
17. Gosheger G, Hardes J, Ahrens H. Silver-coated megaendoprostheses in a rabbit model-an analysis of the infection rate and toxicological side effects. Biomaterials. 2004; 25:5547-56. https://doi.org/10.1016/j.biomaterials.2004.01.008
18. Rupp ME, Fitzgerald T, Marion N. Effect of silver-coated urinary catheters: efficacy, cost effectiveness, and antimicrobial resistance. American Journal of Infection Control. 2004, 32: 445-450. https://doi.org/10.1016/j.ajic.2004.05.002
19. Ohashi S, Saku S, Yamamoto K. Antibacterial activity of silver inorganic agent YDA filler. Journal of Oral Rehabilitation. 2004; 31: 364-7. https://doi.org/10.1111/j.1365-2842.2004.01200.x
20. Bosetti M, Masse A, Tobin E, Cannas M. Silver coated materials for external fixation devices: in vitro biocompatibility and genotoxicity. Biomaterials. 2002; 23: 887-892. https://doi.org/10.1016/S0142-9612(01)00198-3
21. Lee HJ, Yeo SY, Jeong SH. Antibacterial effect of nanosized silver colloidal solution on textile fabrics. J. Mater. Sci. 2003; 38: 2199-2204. https://doi.org/10.1023/A:1023736416361
22. Sun Y, Xia Y. Shape-Controlled Synthesis of Gold and Silver Nanoparticles. Science. 2002; 298: 2176-9. https://doi.org/10.1126/science.1077229
23. Zargar M, Hamid AA, Bakar FA, Shamsudin MN, Shameli K, Jahanshiri F, Farahani F. Green synthesis and antibacterial effect of silver nanoparticles using Vitex negundo L. Molecules. 2011; 16: 6667-76. https://doi.org/10.3390/molecules16086667
24. Verma A, Mehata MS. Controllable synthesis of silver nanoparticles using Neem leaves and their antimicrobial activity. Journal of Radiation Research and Applied Sciences 2016; 9: 109-115. https://doi.org/10.1016/j.jrras.2015.11.001
25. Yan-yu R, Huia Y, Tao W, Chuang W. Green synthesis and antimicrobial activity of monodisperse silver nanoparticles synthesized using Ginkgo Bilobaleaf extractPhysics Letters A 2016; 380: 3773-3777.
26. Perez C, Pauli M, Bazerque P. An antibiotic assay by agar-well diffusion method. Acta Biologiae et Medicinal Experimentalis. 1990; 15:113-5.
27. Talibi I, Askarne L, Boubaker H, Boudyach EH, Msanda F, Saadi B. Antifungal activity of some Moroccan plants against Geotrichum candidum, causal agent of postharvest citrus sour rot. Crop Prot. 2012; 35:41-6. https://doi.org/10.1016/j.cropro.2011.12.016
28. Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, Fatma A. Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids Surf B. 2010; 81: 81-6. https://doi.org/10.1016/j.colsurfb.2010.06.029
29. Parsons JG, Peralta-Videa JR, Gardea-Torresdey JL. Use of plants in biotechnology: Synthesis of metal nanoparticles by inactivated plant tissues, plant extract, and living plants. In: Sarkar D, Datta R, Hannigan R editors. Developments in Environmental Science 2005; 5: p. 463-85.
30. Kumar V, Yadav SC, Yadav SK. Syzygium cumini leaf and seed extract mediated biosynthesis of silver nanoparticles and their characterization. J Chem Technol Biotechnol. 2010; 85:1301-9. https://doi.org/10.1002/jctb.2427
31. Jain D, Kumar D, Kachhwaha S, Kothari S. Synthesis of plant mediated silver nanoparticles using Papaya Fruit Extract and Evaluation of their Antimicrobial Activities. Digest. Journal of Nanomaterials and Biostructures. 2009; 4:557-63.
32. Rai A, Singh A, Sastry M. Role of halide ions and temperature on the morphology of biologically synthesized gold nanotriangles. Langmuir. 2006; 22:736-41. https://doi.org/10.1021/la052055q
33. Noginov MA, Zhu G, Bahuoura M, Adegoke J, Ritzo BA. The effect of gain and absorption on surface Plasmon in metal nanoparticles. Appl. Phys. B. 2006; 86:455-60. https://doi.org/10.1007/s00340-006-2401-0
34. Roopan S, Rohit M, Rahuman A,. Kamaraj C, Bharathi A. Low-cost and ecofriendly phyto-synthesis of silver nanoparticles using Cocos nucifera coir extract and its larvicidal activity. Industrial Crops and Products. 2013; 43:631-5. https://doi.org/10.1016/j.indcrop.2012.08.013
35. Kelly KL, Coronado E, Zhao LL, Schatz GC. The optical properties of metal nanoparticles: The influence of size, shape and dielectric environment. J. Phys. Chem. B. 2003; 107:668-77. https://doi.org/10.1021/jp026731y
36. Kreibig U, Vollmer M. Optical Properties of Metal Clusters; Springer: Berlin, Germany. 1995; p.14-41. https://doi.org/10.1007/978-3-662-09109-8
37. Rastogi L, Arunachalam J. Sunlight based irradiation strategy for rapid green synthesis of highly stable silver nanoparticles using aqueous garlic (Allium sativum) extract and their antibacterial potential. Mater. Chem. Phys. 2011; 129:558-63. https://doi.org/10.1016/j.matchemphys.2011.04.068
38. Suman TY, Rajasree SRR, Kanchana A, Elizabeth SB. Biosynthesis, characterization and cytotoxic effect of plant mediated silver nanoparticles using Morinda citrifolia root extract. Colloids Surf. B Biointerfaces. 2013; 106:74-8. https://doi.org/10.1016/j.colsurfb.2013.01.037
39. Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M. Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant Extract. Biotechnology progress. 2006; 22: 577-83. https://doi.org/10.1021/bp0501423
40. Morones JR, Elechiguerra JL, Camacho A. The bactericidal effect of silver nanoparticles. Nanotechnology. 2005; 16: 2346-53. https://doi.org/10.1088/0957-4484/16/10/059
41. Reddy NJ, Vali DN, Rani M, Rani SS. Evaluation of antioxidant, antibacterial and cytotoxic effects of green synthesized silver nanoparticles by Piper longum fruit. Mater. Sci. Eng. C. 2014; 34:115-22. https://doi.org/10.1016/j.msec.2013.08.039
42. Lee HY, Park HK, Lee YM, Kim K, Park SB. Silver nanoparticles and its antibacterial evaluation for biomedical applications. Chem. Comm. 2007; 28: 2885.
43. Pal S, Tak YK, Song JM. Does the antibacterial activity of silver nanoparticles depend on the shape of the Nanoparticle? A study of the gram-negative bacterium Escherichia coli. Appl. Environ. Microbiol. 2007; 73:1712-20. https://doi.org/10.1128/AEM.02218-06
44. Vivek M, Kumar PS, Steffi S, Sudha S. Biogenic silver nanoparticles by Gelidiella acerosa extract and their antifungal effects. Avicenna J. Med. Biotechnol. 2011; 3:143.
45. Dibrov P, Dzioba J, Gosink KK, Hase CC. Chemiosmotic mechanism of antimicrobial activity of Ag (+) in Vibrio cholerae. Antimicrob. Agents Chemother. 2002; 46: 2668-70. https://doi.org/10.1128/AAC.46.8.2668-2670.2002
46. Kumar KM, Mandal BK, Sinha M, Krishnakumar V. Terminalia chebula mediated green and rapid synthesis of gold nanoparticles. Spectrochim. Acta A: Mol. Biomol. Spectrosc. 2012; 86:490-494. https://doi.org/10.1016/j.saa.2011.11.001
47. Udayakumar R, Velmurugan K, Srinivasan D, Krishna RR. Phytochemical and antimicrobial studies of extracts of Solanum Xanthocarpum. Ancient Science of Life. 2003; 23:1-7.
48. Saha S, Dhanasekaran D, Chandraleka S, Panneerselvam A. Synthesis, characterization and antimicrobial activity of cobalt metal complex against multi drug resistant bacterial and fungal pathogens. Facta Universitatis Series Phys. Chem. Technol. 2009; 7:73-80. https://doi.org/10.2298/FUPCT0901073S
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11-09-2017
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Shukla K, Giri B, Dwivede R. Efficient synthesis of plant-mediated silver nanoparticles and their screening for antimicrobial activity. Plant Sci. Today [Internet]. 2017 Sep. 11 [cited 2024 Nov. 21];4(3):143-50. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/328
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